Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00779217
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 7698nts
Open reading frame: 1 to 7698
ATGGCCATCTCATCACGCCTCGCCCTGTGGGAGCAGAAGATTCGGGAAGAGGACAAGAGC
CCTCCACCATCCTCGCCCCCTCCTCTTTTCTCTGTCATCCCAGGGGGCTTCATTAAGCAA
CTGGTCCGGGGGACTGAAAAAGAGGCCAAGGAAGCGAGACAGAGGAAGCAGTTAGCTGTC
GCCTCTCCAGAACGAGAGATCCCAGAAATTTCCATCAGCCAACCCAACAGCAAGTCCAGC
AGTGGCACCAGATCTGGAAGCCAGCAGATCTCTCAGGACGACCAGTCAAGCTCTCCTGGG
AGCTCAGACATTCTGGGCAAGGAGAGCGAGGGGTCCCGCAGCCCCGACCCTGAGCAGATG
ACAAGCATCAATGGTGAGAAGGCCCAGGAGCTGGGCTCCAGTGCGACACCAACCAAAAAG
ACTGTCCCCTTCAAGAGGGGCGTGAGGAGGGGTGATGTGTTGTTGATGGTGGCCAAGCTG
GACCCGGACTCAGCCAAGCCAGAGAAGACTCATCCCCATGACGCCCCCCCTTGCAAGACC
TCTCCCCCCGCCACAGATACTGGAAAGGAAAAGAAAGGGGAGACCTCTAGGACTCCTTGT
GGCTCCCAGGCCAGCACCGAGATCTTGGCCCCGAAAGCTGAGAAGACCCGGACTGGGGGT
CTTGGGGACCCAGGCCAAGGAACTGTGGCACTGAAAAAAGGCGAGGAGGGTCAAAGCATA
GTGGGGAAGGGGCTTGGGACCCCCAAGACCACAGAGCTGAAAGAGGCTGAGCCCCAGGGC
AAAGACAGGCAGGGGACCAGGCCCCAAGCCCAAGGGCCCGGCGAGGGGGTGCGACCAGGG
AAAGCAGAGAAGGAGGGAGCAGAGCCCACAAACACGGTGGAAAAGGGGAATGTCTCTAAG
GACGTAGGGAGTGAAGGGAAGCACGTAAGGCCCCAAATCCCTGGGAGAAAGTGGGGAGGT
TTCCTGGGAAGAAGGAGTAAGTGGGACGGTCCCCAGAATAAGAAGGACAAAGAAGGGGTG
CTCTTAAGTAAGGCAGAGAAGACAGGTGAGCCTCAGACCCAGATGGAGAAGACAAGCCAA
GTGCAGGGCGAGTTGGGGGACGATCTGAGAATGGGGGAGAAAGCAGGTGAGCTTCGGAGC
ACGACTGGGAAGGCAGGTGAGTCCTGGGATAAGAAGGAAAAGATGGGGCAACCCCAGGGT
AAGTCCGGGAACGCAGGTGAAGCTCGGAGTCAGACAGAGAAGGGCTGTGAAGCCCCAAAG
GAGGTGAGCACAATGGTGGAGTCGCCAGCAGCTCCTGGGAAGGGAGGCTGGCCAGGAAGC
CGTGGGCAGGAAGCAGAGGAGCCCTGCTCAAGAGCAGGTGATGGGGCTGGTGCCCTGGAG
ACAGAGCTGGAAGGACCCAGCCAGCCTGCTCTGGAGAAGGATGCAGAAAGGCCTCGGATA
CGGAAGGAGAACCAAGACGGGCCAGCCCCGCAGGAGGAGGGCAAAGGAGGCCAGAGCAGA
GACTCAGACCAGGCTCCTGAGGACAGATGGTATGAGGCAGAGAAAGTCTGGCTGGCTCAG
AAGGATGGATTTACTCTTGCTACGGTGCTAAAGCCAGATGAGGGAACAGCAGACCTGCCA
GCAGGAAGGGTGAGACTTTGCATTGATGCTGACAAAACCATCACTGAGGTGGATGAGGAG
CATGTCCATCGGGCCAACCCTCCTGAGCTGGACCAGGTCGAGGACCTGGCCTCTCTCATC
AGTGTCAACGAATCCAGTGTCCTGAACACGCTTCTGCAGCGCTACAAAGCTCAGCTGCTG
CACACCTGCACAGGGCCTGATCTGATTGTCCTCCAGCCCCGGGGGCCCTCGGTGCCTTCT
GCAGGGAAGGTGCCCAAGGGCCGCCGGGATGGCCTGCCTGCCCACATTGGCTCCATGGCA
CAGCGGGCATACTGGGCGCTGCTGAACCAGCGGAGAGACCAGAGCATTGTGGCCCTGGGC
CGGAGTGGCGCTGGGAAGACCACCTGCTGTGAGCAGGTCCTGGAACACCTGGTGGGGATG
GCAGGCAGTGTGGATGGCAGGGTCTCAGTGGAGAAGATCCGAGCCACCTTCACTGTCCTC
CGGGCCTTCGGCTCTGTGTCCATGGCCCACAGCCGCAGTGCCACCCGGTTCTCCATGGTG
ATGTCGCTGGACTTCAACGCTACAGGCCGCATCACAGCTGCTCAGCTCCAGACAATGCTT
TTGGAGAAGAGCCGCGTGGCACGGCAGCCGGAAGGGGAAAGTAACTTCCTGGTTTTCTCC
CAGATGCTGGCTGGATTGGACTTGGATCTCAGGACGGAGCTGAACCTGCACCAGATGGCA
GATAGCAGCTCCTTTGGCATGGGCGTGTGGTCCAAGCCTGAAGATAAACAGAAGGCGGCA
GCTGCCTTTGCCCAGCTCCAGGGTGCCATGGAGATGCTGGGCATCTCAGAGAGCGAGCAG
CGGGCTGTTTGGCGGGTCCTGACAGCCATCTACCACCTGGGCGCGGCGGGGGCCTGCAAA
GTGGGTCGGAAGCAGTTCATGAGGTTTGAGTGGGCAAACTACGCAGCTGAGGCCCTGGGC
TGCGAGTATGAGGAGCTGAACACGGCCACCTTCAAGCACCACCTTCGACAGATCATCCAG
CAAATGACGTTTGGGCCAAGCCGATGGGGCCTCGAGGATGAGGAAACCAGCTCAGGGCTC
AAGATGACAGGAGTGGACTGTGTGGAGGGGATGGCCTCGGGCCTGTACCAGGAACTCTTT
GCGGCTGTGGTCTCACTCATCAACAGATCCTTTTCCTCCCACCATCTCTCCATGGCCTCC
ATCATGGTGGTGGACTCTCCAGGCTTCCAGAACCCCCGGCACCAGGGCAAGGACCGGGCG
GCCACCTTTGAGGAGCTGTGCCACAACTACGCCCATGAGCGCCTGCAGCTGCTGTTCTAC
CAGCGGACCTTTGTCTCCACGCTACAGCGATATCAAGAGGAAGGTGTTCCTGTGCAGTTT
GACCTCCCGGACCCCTCCCCAGGGACCACCGTGGCTGTTGTGGATCAAAATCCCTCTCAG
GTCCGCTTACCAGCTGGAGGAGGTGCCCAGGATGCCAGAGGCCTTTTCTGGGTCTTAGAT
GAGGAAGTCCATGTAGAGGGCTCCAGTGACAGTGTGGTGCTCGAGCGTCTGTGTGCTGCT
TTCGAGAAGAAAGGAGCTGGGACTGAAGGGTCCTCTGCCCTGCGGACCTGTGAGCAGCCC
CTCCAGTGTGAGATTTTCCACCAGTTGGGATGGGACCCTGTGCGGTACGACCTCACGGGC
TGGCTCCACAGAGCCAAGCCCAACCTCTCGGCCCTGGATGCACCCCAGGTCCTGCAACAG
TCAAAAAGAGAGGAGCTGCGGAGTCTATTCCAGGCCCGGGCCAAGCTGCCTCCTGTGTGC
CGGGCTGTGGCAGGCCTGGAGGGCACCTCCCAGCAGGCCCTGCAGAGGAGCCGCATGGTG
AGGAGGACCTTTGCCAGCAGCCTTGCCGCGGTGAGGAGGAAAGCCCCGTGCTCCCAGATC
AAGCTGCAGATGGATGCGCTGACCAGCATGATCAAAAGGTCCCGGCTGCACTTTATCCAC
TGCCTGGTACCAAACCCTGTGGTGGAAAGCAGGAGTGGGCAGGAATCTCCACCACCACCG
CAGCCTGGTAGAGACAAGCCTGGGGCAGGTGGACCTCTGGCCCTGGATATCCCAGCACTG
AGGGTCCAGCTTGCTGGGTTCCACATCCTGGAGGCTCTGCGTCTGCATAGGACAGGCTAT
GCTGACCACATGGGACTCACTCGCTTCCGCCGGCAATTCCAGGTGCTGGACGCTCCACTC
CTGAAGAAGCTCATGTCGACCTCCGAGGGAATAGATGAAAGGAAGGCCGTGGAGGAGCTC
CTGGAGACCCTGGATCTGGAAAAGAAGGCGGTGGCTGTGGGGCACAGCCAAGTTTTTCTC
AAGGCAGGTGTGATCTCCAGGCTTGAGAAGCAGCGAGAGAAGCTGGTATCTCAGAGCATC
GTTCTCTTCCAGGCGGCTTGCAAGGGCTTTCTGTCTCGCCAGGAATTCAAGAAGCTGAAG
ATTCGCCGACTGGCTGCACAGTGCATCCAGAAGAATGTGGCTGTGTTCCTCGCAGTCAAG
GACTGGCCATGGTGGCAGCTGCTTGGTTCCCTCCAGCCTCTACTTAGTGCCACCATTGGA
ACTGAGCAGCTCCGAGCCAAGGAGGAGGAGCTTACAACGCTAAGACGGAAGCTAGAAAAA
TCAGAGAAGTTGCGGAATGAACTCCGGCAGAACACAGATCTGCTAGAAAGCAAGATTGCT
GACTTGACCTCTGACCTTGCCGATGAGCGCTTCAAAGGTGATGTGGCCTGCCAGGTGCTG
GAGAGTGAGCGGGCAGAGCGGCTACAGGCCTTCCGGGAGGTCCAGGAGCTCAAGAGCAAG
CATGAACAAGTCCAGAAAAAACTGGGAGATGTGAATAAACAGTTGGAAGAAGCCCAGCAG
AAAATTCAGTTGAATGACTTGGAAAGGAATCCCACTGGAGGAGACGAGTGGCAGATGCGC
TTCGACTGTGCTCAGATGGAGAACGAGTTCCTCAGAAAGCGTCTGCAGCAATGCGAGGAG
AGGCTGGACTCGGAGCTGACAGCCAGGAAAGAGCTGGAGCAAAAGCTTGGGGAGTTGCAA
AGTGCTTATGACGGGGCCAAGAAGATGGCTCACCAACTGAAGAGGAAGTGCCACCATCTT
ACCTGTGACCTTGAGGATACCTGCGTCCTGCTAGAGAACCAACAAAGTCGAAACCATGAG
CTGGAGAAGAAGCAGAAGAAGTTTGACCTGCAGCTGGCCCAGGCCCTAGGTGAGTCAGTG
TTTGAGAAGGGTCTCCGTGAGAAAGTGACCCAGGAGAACACCAGTGTCCGGTGGGAGCTA
GGCCAGCTTCAGCAGCAGCTGAAGCAAAAGGAGCAGGAAGCCTCACAGCTGAAGCAGCAG
GTGGAGATGCTACAGGACCATAAACGGGAGCTGCTGGGGTCACCCTCTCTGGGGGAAAAT
TGCGTTGCTGGCTTGAAGGAGAGGCTCTGGAAGTTGGAATCCAGCGCCCTTGAGCAACAG
AAAATCCAGAGCCAGCAGGAAAACACCATCAAGCAGCTGGAGCAGCTCCGCCAGCGGTTT
GAGCTGGAGATCGAGCGGATGAAGCAGATGCACCAGAAGGACCGTGAGGACCAGGAGGAG
GAACTGGAGGATGTCCGTCAGTCCTGCCAGAAGCGGCTTCATCAGCTGGAAATGCAGCTG
GAGCAAGAGTATGAAGAGAAGCAGATGGTCCTCCATGAGAAGCAAGATTTGGAAGGCTTG
ATCGGAACCCTCTGTGACCAGATTGGCCATCGGGACTTTGATGTGGAGAAGCGACTTCGG
AGAGACCTCAGGAGGACACATGCACTGTTGTCAGACGTGCAGCTCCTTCTGGGCACCATG
GAGGATGGCAAGACATCAGTCAGCAAGGAGGAGCTGGAGAAAGTGCACAGCCAGCTGGAG
CAGAGTGAAGCCAAGTGTGAGGAGGCCTTGAAGACGCAGAAGGTGCTCACAGCGGACCTG
GAGAGTATGCACAGCGAGCTGGAGAACATGACGCGGAACAAGAGCCTGGTGGATGAGCAG
CTGTACAGGCTGCAGTTTGAGAAGGCGGACCTCCTGAAGCGCATCGATGAGGACCAGGAT
GACCTGAATGAGCTGATGCAGAAGCACAAGGACCTCATTGCTCAGTCTGCTGCTGACATT
GGGCAGATCCAAGAACTGCAGCTGCAGCTGGAGGAAGCCAAGAAGGAGAAGCACAAGCTA
CAAGAACAACTGCAGGTGGCTCAGATGCGCATCGAGTACCTGGAACAGTCCACCGTGGAT
CGAGCCATCGTCAGCAGGCAGGAGGCGGTCATCTGTGACCTAGAGAACAAGACAGAGTTC
CAGAAGGTGCAGATTAAGAGATTTGAGGTCCTGGTGATCCGGCTTCGGGACAGCCTGATC
AAGATGGGGGAGGAGCTTTCACAGGCGGCCACCTCCGAGTCCCAGCAGCGGGAGAGCAGC
CAGTACTACCAGCGGCGCCTGGAAGAGCTGAAGGCCGACATGGAAGAGCTGGTGCAGCGG
GAGGCAGAGGCCAGCCGGCGGTGCATGGAGCTGGAGAAGTACGTGGAGGAACTTGCAGCA
GTGAGGCAAACCCTCCAGACAGACCTGGAGACATCCATTCGGCGGATTGCCGACCTGCAG
GCTGCCTTGGAAGAAGTGGCATCCAGTGACAGTGATACTGAGAGTGTCCAGACGGCAGTG
GATTGTGGCAGCAGCGGCCGAAAAGAGATGGATAACGTCTCCATCCTCAGCTCCCAGCCA
GAGGGCAGCCTGCAGTCCTGGTTGAGCTGTACTCTGTCCCTGGCCACAGATACTATGAGG
ACTCCTTCTCGACAGTCAGCCACCAGCAGCCGCATCCTCAGCCCCAGGATAAACGAAGAG
GCTGGGGACACTGAGAGGACCCAGTCGGCATTGGCACTGAGCAGAGCCCGGTCCACCAAT
GTCCACAGCAAGACCTCAGGAGACAAGCCTGTTTCTCCCCACTTTGTCCGCCGGCAAAAG
TACTGTCATTTTGGGGACGGCGAAGTGCTTGCCGTCCAGAGAAAGTCCACAGAGAGATTA
GAACCTGCTTCCTCTCCCCTGGCTTCTCGGAGTACAAATACATCCCCGCTGTCGAGGGAA
AAGCTGCCCAGTCCTTCAGCGGCCCTCTCGGAGTTCGTGGAAGGGCTCCGGAGGAAGAGA
GCCCAGAGAGGCCAGGGGTCCACGCTGGGCCTAGAGGACTGGCCCACTCTCCCCATTTAC
CAGACGACTGGGGCCTCCACACTAAGGAGGGGCAGGGCTGGCAGTGACGAGGGAAACCTC
TCGCTGAGGGTTGGGGCAAAGTCACCCCTGGAAATCGAAGGGGCCGCTGGTGGTCTCTTG
AGGTCCACCAGCCTCAAATGCATCTCTTCAGACGGTGTTGGGGGCACAACCCTACTCCCC
GAAAAGTCGAAAACCCGATTCAGTTCCTGCGAGTCCCTCTTAGAATCCAGACCGAGCATG
GGGAGAAAACTGAGCTCTCCGACCACACCCAGGGACATGCTGTTGTCGCCCACACTGCGT
CCTCGGAGGCGGTGTCTGGAGTCCTCTGTGGACGATGCGGGCTGTCCAGACCTTGGAAAG
GAGCCGCTTGTTTTCCAGAACCGCCAGTTTGCCCACCTGATGGAGGAACCTCTAGGCAGT
GACCCATTCAGCTGGAAACTCCCAAGCCTCGACTACGAACGCAAGACCAAAGTGGACTTC
GATGACTTCCTCCCAGCTATCCGGAAGCCCCAGACACCTACCTCCTTGGCTGGATCAGCC
AAAGGTGGGCAAGACGGTTCACAGCGTTCAAGCATCCACTTTGAAACGGAAGAGGCTAAC
CGTTCCTTTCTCTCGGGGATCAAGACCATTTTGAAGAAGAGCCCGGAGCCCAAGGAGGAT
CCCGCTCACCTGTCTGACTCGTCCTCATCCTCCGGCTCCATCGTGTCCTTCAAAAGTGCT
GACAGCATCAAAAGTCGACCAGGAATCCCACGACTTGCGGGTGACGGTGGCGAGCGAACG
TCCCCCGAGCGGAGAGAGCCAGGGACGGGGAGGAAAGACGACGATGTTGCGAGCATAATG
AAGAAATACCTCCAGAAG
Protein sequence predicted based on nucleotide sequence
MAISSRLALWEQKIREEDKSPPPSSPPPLFSVIPGGFIKQLVRGTEKEAKEARQRKQLAV ASPEREIPEISISQPNSKSSSGTRSGSQQISQDDQSSSPGSSDILGKESEGSRSPDPEQM TSINGEKAQELGSSATPTKKTVPFKRGVRRGDVLLMVAKLDPDSAKPEKTHPHDAPPCKT SPPATDTGKEKKGETSRTPCGSQASTEILAPKAEKTRTGGLGDPGQGTVALKKGEEGQSI VGKGLGTPKTTELKEAEPQGKDRQGTRPQAQGPGEGVRPGKAEKEGAEPTNTVEKGNVSK DVGSEGKHVRPQIPGRKWGGFLGRRSKWDGPQNKKDKEGVLLSKAEKTGEPQTQMEKTSQ VQGELGDDLRMGEKAGELRSTTGKAGESWDKKEKMGQPQGKSGNAGEARSQTEKGCEAPK EVSTMVESPAAPGKGGWPGSRGQEAEEPCSRAGDGAGALETELEGPSQPALEKDAERPRI RKENQDGPAPQEEGKGGQSRDSDQAPEDRWYEAEKVWLAQKDGFTLATVLKPDEGTADLP AGRVRLCIDADKTITEVDEEHVHRANPPELDQVEDLASLISVNESSVLNTLLQRYKAQLL HTCTGPDLIVLQPRGPSVPSAGKVPKGRRDGLPAHIGSMAQRAYWALLNQRRDQSIVALG RSGAGKTTCCEQVLEHLVGMAGSVDGRVSVEKIRATFTVLRAFGSVSMAHSRSATRFSMV MSLDFNATGRITAAQLQTMLLEKSRVARQPEGESNFLVFSQMLAGLDLDLRTELNLHQMA DSSSFGMGVWSKPEDKQKAAAAFAQLQGAMEMLGISESEQRAVWRVLTAIYHLGAAGACK VGRKQFMRFEWANYAAEALGCEYEELNTATFKHHLRQIIQQMTFGPSRWGLEDEETSSGL KMTGVDCVEGMASGLYQELFAAVVSLINRSFSSHHLSMASIMVVDSPGFQNPRHQGKDRA ATFEELCHNYAHERLQLLFYQRTFVSTLQRYQEEGVPVQFDLPDPSPGTTVAVVDQNPSQ VRLPAGGGAQDARGLFWVLDEEVHVEGSSDSVVLERLCAAFEKKGAGTEGSSALRTCEQP LQCEIFHQLGWDPVRYDLTGWLHRAKPNLSALDAPQVLQQSKREELRSLFQARAKLPPVC RAVAGLEGTSQQALQRSRMVRRTFASSLAAVRRKAPCSQIKLQMDALTSMIKRSRLHFIH CLVPNPVVESRSGQESPPPPQPGRDKPGAGGPLALDIPALRVQLAGFHILEALRLHRTGY ADHMGLTRFRRQFQVLDAPLLKKLMSTSEGIDERKAVEELLETLDLEKKAVAVGHSQVFL KAGVISRLEKQREKLVSQSIVLFQAACKGFLSRQEFKKLKIRRLAAQCIQKNVAVFLAVK DWPWWQLLGSLQPLLSATIGTEQLRAKEEELTTLRRKLEKSEKLRNELRQNTDLLESKIA DLTSDLADERFKGDVACQVLESERAERLQAFREVQELKSKHEQVQKKLGDVNKQLEEAQQ KIQLNDLERNPTGGDEWQMRFDCAQMENEFLRKRLQQCEERLDSELTARKELEQKLGELQ SAYDGAKKMAHQLKRKCHHLTCDLEDTCVLLENQQSRNHELEKKQKKFDLQLAQALGESV FEKGLREKVTQENTSVRWELGQLQQQLKQKEQEASQLKQQVEMLQDHKRELLGSPSLGEN CVAGLKERLWKLESSALEQQKIQSQQENTIKQLEQLRQRFELEIERMKQMHQKDREDQEE ELEDVRQSCQKRLHQLEMQLEQEYEEKQMVLHEKQDLEGLIGTLCDQIGHRDFDVEKRLR RDLRRTHALLSDVQLLLGTMEDGKTSVSKEELEKVHSQLEQSEAKCEEALKTQKVLTADL ESMHSELENMTRNKSLVDEQLYRLQFEKADLLKRIDEDQDDLNELMQKHKDLIAQSAADI GQIQELQLQLEEAKKEKHKLQEQLQVAQMRIEYLEQSTVDRAIVSRQEAVICDLENKTEF QKVQIKRFEVLVIRLRDSLIKMGEELSQAATSESQQRESSQYYQRRLEELKADMEELVQR EAEASRRCMELEKYVEELAAVRQTLQTDLETSIRRIADLQAALEEVASSDSDTESVQTAV DCGSSGRKEMDNVSILSSQPEGSLQSWLSCTLSLATDTMRTPSRQSATSSRILSPRINEE AGDTERTQSALALSRARSTNVHSKTSGDKPVSPHFVRRQKYCHFGDGEVLAVQRKSTERL EPASSPLASRSTNTSPLSREKLPSPSAALSEFVEGLRRKRAQRGQGSTLGLEDWPTLPIY QTTGASTLRRGRAGSDEGNLSLRVGAKSPLEIEGAAGGLLRSTSLKCISSDGVGGTTLLP EKSKTRFSSCESLLESRPSMGRKLSSPTTPRDMLLSPTLRPRRRCLESSVDDAGCPDLGK EPLVFQNRQFAHLMEEPLGSDPFSWKLPSLDYERKTKVDFDDFLPAIRKPQTPTSLAGSA KGGQDGSQRSSIHFETEEANRSFLSGIKTILKKSPEPKEDPAHLSDSSSSSGSIVSFKSA DSIKSRPGIPRLAGDGGERTSPERREPGTGRKDDDVASIMKKYLQK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
