Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00779790
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3315nts
Open reading frame: 1 to 3315
GCCGCAGCGGCGGGCGGCGGCGGGCCGGGGACAGCGGTAGGCGCCACGGGCTCGGGGATT
GCGGCGGCAGCCGCAGGCCTAGCTGTTTATCGACGGAAGGATGGGGGCCCGGACACCAAG
TTTTGGGAGAGCCCGGAGACGGTGTCCCAGCTGGATTCGGTGCGGGTCTGGCTGGGCAAG
CACTACAAGAAGTATGTTCATGCGGATGCTCCTACCAATAAAACACTGGCTGGGCTGGTG
GTGCAGCTTCTTCAGTTCCAGGAAGATGCCTTTGGGAAGCATGTCACCAACCCGGCCTTC
ACCAAACTCCCTGCAAAGTGTTTCATGGATTTCAAAGCTGGAGGCGCCTTATGTCACATT
CTTGGGGCTGCTTACAAGTATAAAAATGAACAGGGATGGCGGAGGTTTGACCTACAGAAC
CCATCTCGAATGGATCGTAATGTGGAAATGTTTATGAACATTGAAAAAACATTGGTGCAG
AACAATTGTTTGACCAGACCCAACATCTACCTCATTCCAGACATTGATCTGAAGTTGGCT
AACAAATTGAAAGATATCATCAAACGACATCAGGGAACATTTACGGATGAGAAGTCAAAA
GCTTCCCACCACATTTACCCATATTCTTCCTCACAAGACGATGAAGAATGGTTGAGACCG
GTGATGAGAAAAGAGAAGCAAGTGTTAGTGCATTGGGGCTTTTACCCAGACAGCTATGAT
ACTTGGGTCCATAGTAATGATGTTGATGCTGAAATTGAAGATCCACCAATTCCAGAAAAA
CCATGGAAGGTTCATGTGAAATGGATTTTGGACACTGATATTTTCAATGAATGGATGAAT
GAGGAGGATTATGAGGTGGATGAAAATAGGAAGCCTGTGAGTTTTCGTCAGCGGATTTCA
ACCAAGAATGAAGAGCCAGTCAGAAGTCCAGAAAGAAGAGATAGAAAAGCATCAGCTAAT
GCTCGAAAGAGGAAACATTCGCCTTCGCCTCCCCCTCCGACACCAACAGAATCACGGAAG
AAGAGTGGGAAGAAAGGCCAAGCTAGCCTTTATGGGAAGCGCAGAAGTCAGAAAGAGGAA
GATGAGCAAGAAGATCTAACCAAGGATATGGAAGACCCAACACCTGTACCCAATATAGAA
GAAGTAGTACTTCCCAAAAATGTGAACCTAAAGAAAGATAGTGAAAATACACCTGTTAAA
GGAGGAACTGTAGCGGATCTAGATGAGCAGGATGAAGAAACAGTCACAGCAGGAGGAAAG
GAAGATGAAGATCCTGCCAAAGGTGATCAGAGTCGATCAGTTGACCTTGGGGAAGATAAT
GTGACAGAGCAGACCAATCACATTATTATTCCTAGTTATGCATCATGGTTTGATTATAAC
TGTATTCATGTGATTGAACGGCGTGCTCTTCCTGAGTTCTTCAATGGAAAAAACAAATCC
AAGACTCCAGAAATATACTTGGCATATCGAAATTTTATGATTGACACGTATCGTCTAAAC
CCCCAAGAGTATTTAACTAGCACTGCTTGTCGGAGGAACTTGACTGGAGATGTGTGTGCT
GTGATGAGGGTCCATGCCTTTTTAGAGCAGTGGGGACTCGTTAATTACCAAGTTGACCCG
GAAAGTAGACCCATGGCAATGGGACCTCCTCCTACTCCTCATTTTAATGTATTAGCTGAT
ACCCCCTCTGGGCTTGTGCCTCTGCATCTTCGATCACCTCAGGTTCCTGCTGCTCAACAG
ATGCTAAATTTTCCTGAGAAAAACAAGGAAAAACCAGTTGATTTGCAGAACTTTGGTCTC
CGTACTGACATTTACTCCAAGAAAACATTAGCAAAGAGTAAAGGTGCTAGTGCTGGAAGA
GAATGGACTGAACAGGAGACCCTTCTACTCCTGGAGGCCCTGGAGATGTACAAGGATGAT
TGGAACAAAGTGTCGGAACATGTTGGAAGTCGTACTCAGGATGAATGCATCCTCCACTTT
TTGAGACTTCCCATTGAGGACCCATACCTTGAGAATTCAGATGCTTCCCTTGGGCCTTTG
GCCTACCAGCCTGTCCCCTTCAGTCAGTCAGGAAATCCAGTTATGAGTACTGTTGCTTTT
TTGGCATCTGTGGTGGACCCTCGCGTGGCATCTGCTGCAGCAAAAGCGGCTTTGGAGGAG
TTTTCTCGGGTCCGGGAGGAGGTACCACTGGAATTGGTTGAAGCTCATGTCAAGAAAGTA
CAAGAAGCAGCACGAGCCTCTGGGAAAGTGGATCCCACCTACGGTCTGGAGAGCAGCTGC
ATTGCAGGCACAGGGCCCGATGAGCCAGAGAAGCTTGAAGGAGCTGAAGAGGAAAAAATG
GAAGCCGACCCTGATGGTCAGCAGCCTGAAAAGGCAGAAAATAAAGTGGAAAATGAAACG
GATGAAGGTGATAAAGCACAAGATGGAGAAAATGAAAAAAATAGTGAAAAGGAACAGGAT
AGTGAAGTGAGTGAGGATACCAAATCAGAAGAAAAGGAGACTGAAGAGAACAAAGAACTC
ACTGATACATGTAAAGAAAGAGAAAGTGATACTGGGAAGAAGAAAGTAGAACATGAAATT
TCCGAAGGAAATGTTGCCACAGCCGCAGCAGCTGCTCTTGCCTCAGCGGCTACCAAAGCC
AAGCACCTGGCTGCAGTGGAAGAAAGAAAGATCAAGTCCCTGGTAGCTCTCTTGGTTGAG
ACACAAATGAAGAAACTAGAGATCAAACTTCGACATTTTGAAGAGCTGGAAACTATCATG
GACAGAGAGAAAGAAGCTCTAGAACAACAGAGGCAGCAGTTGCTTACTGAACGCCAAAAC
TTCCACATGGAACAGCTGAAGTATGCTGAATTACGAGCACGACAGCAAATGGAACAGCAG
CAGCATGGCCAGAACCCTCAACAGGCACACCAGCACTCAGGAGGACCTGGCCTGGCCCCA
CTTGGAGCAGCAGGGCACCCTGGCATGATGCCTCATCAACAGCCCCCTCCCTACCCTCTG
ATGCACCACCAGATGCCACCACCTCATCCACCCCAGCCAGGTCAGATACCAGGCCCAGGT
TCCATGATGCCCGGGCAGCACATGCCAGGCCGCATGATTCCCACTGTTGCAGCCAACATC
CACCCCTCTGGGAGTGGCCCTACCCCTCCTGGCATGCCACCAATGCCAGGAAACATCTTA
GGACCCCGGGTACCCCTGACAGCACCTAACGGCATGTATCCCCCTCCACCACAGCAGCAG
CCACCGCCACCACCACCTGCAGATGGGGTCCCTCCGCCTCCTGCTCCTGGCCCGCCAGCC
TCAGCTGCTCCTTGG
Protein sequence predicted based on nucleotide sequence
AAAAGGGGPGTAVGATGSGIAAAAAGLAVYRRKDGGPDTKFWESPETVSQLDSVRVWLGK HYKKYVHADAPTNKTLAGLVVQLLQFQEDAFGKHVTNPAFTKLPAKCFMDFKAGGALCHI LGAAYKYKNEQGWRRFDLQNPSRMDRNVEMFMNIEKTLVQNNCLTRPNIYLIPDIDLKLA NKLKDIIKRHQGTFTDEKSKASHHIYPYSSSQDDEEWLRPVMRKEKQVLVHWGFYPDSYD TWVHSNDVDAEIEDPPIPEKPWKVHVKWILDTDIFNEWMNEEDYEVDENRKPVSFRQRIS TKNEEPVRSPERRDRKASANARKRKHSPSPPPPTPTESRKKSGKKGQASLYGKRRSQKEE DEQEDLTKDMEDPTPVPNIEEVVLPKNVNLKKDSENTPVKGGTVADLDEQDEETVTAGGK EDEDPAKGDQSRSVDLGEDNVTEQTNHIIIPSYASWFDYNCIHVIERRALPEFFNGKNKS KTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNLTGDVCAVMRVHAFLEQWGLVNYQVDP ESRPMAMGPPPTPHFNVLADTPSGLVPLHLRSPQVPAAQQMLNFPEKNKEKPVDLQNFGL RTDIYSKKTLAKSKGASAGREWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHF LRLPIEDPYLENSDASLGPLAYQPVPFSQSGNPVMSTVAFLASVVDPRVASAAAKAALEE FSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPEKLEGAEEEKM EADPDGQQPEKAENKVENETDEGDKAQDGENEKNSEKEQDSEVSEDTKSEEKETEENKEL TDTCKERESDTGKKKVEHEISEGNVATAAAAALASAATKAKHLAAVEERKIKSLVALLVE TQMKKLEIKLRHFEELETIMDREKEALEQQRQQLLTERQNFHMEQLKYAELRARQQMEQQ QHGQNPQQAHQHSGGPGLAPLGAAGHPGMMPHQQPPPYPLMHHQMPPPHPPQPGQIPGPG SMMPGQHMPGRMIPTVAANIHPSGSGPTPPGMPPMPGNILGPRVPLTAPNGMYPPPPQQQ PPPPPPADGVPPPPAPGPPASAAPW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
