Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00780004
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 1860nts
Open reading frame: 273 to 2132
GACACGCGAGGTCAGGAAGAAGCCGCTTATAAATTACCGCTTCCTTCGCGCCGCCGCCAA
CGCCGAGCCCCGAGGACCGCAAGCCCAGAGGACAAGCTGCGCCAAGAGGGAGTGCGGAGC
GTTCACCCAGCGGGTCAGAGAGCGAGCGGGCAGGCAGCCCCCGGCCGGCGGAACCCGGCA
CAGCCGAGCAGAGCGCGGGCGGCGCCGCAGCCACCCCAGATCCAGAACCAGAACCACAGC
CCTTCTGAGGAGCTCCCAAACAAAGCAAGGAGATGGCCACCAAGGAGAAGCTGCAGTGTC
TGAAAGATTTCCACAAGGACATCCTGAAGCCCTCACCAGGGAAGAGCCCAGGCACGCGGC
CTGAGGACGAGGCTGAGGGAAAACCTCCGCAGAGGGAGAAGTGGTCTAGCAAGATCGACT
TTGTGCTCTCTGTGGCTGGCGGCTTCGTGGGCTTGGGCAACGTCTGGCGCTTCCCGTACC
TCTGCTACAAGAATGGTGGAGGTGCGTTTCTCATACCGTATTTTATTTTCCTGTTTGGGA
GCGGCCTGCCTGTGTTTTTCTTGGAGATCATCATAGGCCAGTACACCTCTGAAGGGGGCA
TCACCTGCTGGGAAAAGATCTGCCCCTTGTTCTCTGGTATCGGCTATGCCTCCGTTGTAA
TTGTGTCCCTCCTGAATGTCTACTACATCGTCATCCTGGCCTGGGCCACATACTACCTGT
TCCAGTCCTTCCAGAAGGAGCTGCCCTGGGCACACTGCAACCACAGCTGGAACACACCTC
ACTGCATGGAGGACACCATGCGCAAGAACAAGAGTGTCTGGATCACCATCAGCTCCACCA
ACTTCACCTCCCCTGTCATCGAGTTCTGGGAGCGCAACGTGCTGAGCTTGTCCCCTGGAA
TCGACCACCCAGGCTCTCTGAAATGGGACCTCGCTCTCTGCCTTCTTTTAGTCTGGCTAG
TGTGTTTCTTCTGCATCTGGAAGGGCGTCAGGTCCACTGGGAAGGTCGTCTACTTCACAG
CCACTTTTCCATTCGCCATGCTCCTGGTGCTGCTGGTCCGAGGGCTGACGCTGCCGGGCG
CGGGCGCAGGCATCAAGTTCTATCTGTATCCTGACATCACCCGCCTTGAGGACCCACAGG
TGTGGATTGACGCTGGGACTCAGATATTCTTCTCTTATGCCATCTGCCTGGGGGCTATGA
CCTCGCTGGGGAGCTACAACAAGTACAAGTATAACTCGTACAGGGACTGTATGCTGCTGG
GATGCCTGAACAGTGGTACCAGTTTTGTGTCTGGCTTCGCAATTTTTTCCATCCTGGGCT
TCATGGCACAAGAGCAAGGGGTGGACATTGCTGATGTGGCTGAGTCAGGTCCTGGCCTGG
CCTTCATTGCCTACCCAAAAGCTGTGACAATGATGCCGCTGCCCACATTTTGGTCCATTC
TTTTTTTTATTATGCTTCTCTTGCTTGGACTGGATAGCCAGTTTGTTGAAGTTGAAGGAC
AGATCACATCCTTGGTTGATCTTTACCCATCCTTCCTAAGGAAGGGTTATCGTCGGGAAA
TCTTCATCGCCTTCGTGTGTAGCATCAGCTACCTGCTGGGGCTGACGATGGTGACGGAGG
GTGGCATGTATGTGTTTCAGCTCTTTGACTACTATGCAGCTAGCGGTGTATGCCTTTTGT
GGGTTGCATTCTTTGAATGTTTTGTTATTGCCTGGATATATGGAGGTGATAACCTTTATG
ATGGTATTGAGGACATGATTGGCTATCGGCCCGGGCCCTGGATGAAGTACAGCTGGGCTG
TGATCACTCCAGTTCTCTGTGTTGGATGTTTCATCTTCTCGCTCGTCAAGTACGTACCCC
TGACCTACAACAAAACATACGTGTACCCCAACTGGGCCATTGGGCTGGGCTGGAGCCTGG
CCCTTTCCTCCATGCTCTGCGTTCCCTTGGTCATCGTCATCCGCCTCTGCCAGACTGAGG
GGCCGTTCCTTGTGAGAGTCAAGTACCTGCTGACCCCAAGGGAACCCAACCGCTGGGCTG
TGGAGCGCGAGGGAGCCACACCTTACAACTCTCGCACCGTCATGAACGGCGCTCTCGTGA
AACCGACCCACATCATTGTGGAGACCATGATGTGAGCTCTCTCGGGTCGACGGGGCCGGC
GGCTTTCCTGCTGTTTACTAACATTAGATTCTCATAGGACCAGGTTTACAGAGCTTTATA
TTTGCACTAGGATTTTTTTTTTTTTGTAATTGTCACAGAAAATGTAATTGTGGGTATGTG
TGCGTGCGTGTGTGTGTGTGTGTGTGTATCGTGTGTGTGTGTTTTGTTTTGATTTGGGGG
ATATTTTGTACAAAAAGAAAACCCACGGGAAGATGTCCGTGGAGAGGCAGAGCTTTCATA
CTGAATTAGATGTATTTTATGGGAATTTGGTAAATTTTTCTTTGTATTTTTTTTTTTACA
TATAAGTATATATACACTTAGAGATTGTCATATACTTTTACCACTTGAATTGATCTTCTT
GCCAGCAATAGATCTCATTTTCAAAAGCAATTCTTCGGTGCTGTGTAGCTGGCAGAAAGT
TCTGTCCAGTAAACGCAGGATGGAATTTTCCTGGGACTCTACACCCATCTTAAGGTGGTA
TACCTTCCAAATCCTGGTTCAGATGGAAGAAATAGCAGGAGAGAGGACCCATTAGCTGGC
AGACCCAGGGGGAAGAAAGGAGGGCTGTGAGGAGATACCTCATTAAACTTGGCTTAGTGA
AGAAGAGAGATGCCAAAGGAATGAACCAACCCTTCACATAAAGGAGACTGGCTGAAGCTG
AATGAGGAGGCCCTATAGCAGAAGTCTGATTCTAAGAGCAGTAGAAACTTGTACCAGAAG
CAAAATCCCACTTTTAATTTTGAGATGGTGAGTGGATAGTCAGTAGACCGTCAGAACCAC
TGGCCAGAGAGGGAGCTGCTAGAGATCCAAGAAGGCTGGCAGGAATGAGGCTCACAACTC
AGCCTCGCAAGAGGTGGCAGAGGCACAGGAGGCCACAGTCCTTCCTGGGGCATTCCAGGC
AGAGAAGGAGCAGAGGCTCTCCCGGCAGGAGCTGGGGTCTCAGGGCTCAGATGAGTCTGT
TGCATTTGAATGGGGTCATAGCAGGTTCTGGTCATTCCCCAAGCAACATCTCAGCATCTC
TTAAAGTTGCCTGCAGGAATGAAGCATGACATACCTGTTGAGGGACTAGGGGAGTGGTGG
GGAGGTGAGTGGACCAAAGGATATAGGCCCCAGGCATGCAGATGGGCCCGGTGTCGGGGA
GGGGTGCTTTCTTTCCTCATCTCCCCACTCCCCACTCTCAGCCTGGGAGACTCCTGCCAA
GCCCTCATTAAAGATGCCACCCTGGGCTGCCCTGGCACCTAGCAAGGCACACCAAGAACA
GCTTTTGAGTCTGTATCCTCCACTGGGGGAAGTGCTCCCAGTTCAGAACAAGGGCAGCCC
GTGGTGCTGACCTAGGATATAACAAAGCTCTTCACTTCAAAACCCCTGCAATAGCTGGGT
TTACAGACATTTACCACCTGCGGACCCAAAAGAGAAGGCCTAGGAGAGTTTTCTAGAAGG
TTGGGATTGTCAGGGTCCTGGCCCCTCAGAACTGGCTTGATCAAGGGCCTTATGTGGAGC
AGAGGTTGTCTCTGAACCAGGAGAGAAGGTACTATACCTTTCAAATCCCCAGGGCAGACA
CACCCCCACCCAGCCCCTATTTGGACCTAAACTGTGCCATTTGAACAGTCACTTCCAAGC
TCAGTCTAAATGAAACCGAAACGTGACCACGCACAAAGGCAGTCACTGCCTCGAGGGGTG
CAGACCGCAGAATTTTCACAGCAGGGGCTCTTGGAACCCTGGAAACCCCCTTCTTAAATT
TGGGAGGAGGAGTATGCCTTTGGTGTCCCCCTCCCAAGGGGCAATTCTGAACCCCATCTT
TGGCAGGCATACATATTTCACTGTTTCCAAAGCTATCTACTCTGCCAAACAACACCCAGT
CCTATTCCAAACTCTCAACAATTCTATCTTGTTCCTGTTTTTCTATGTATTTATGGTTGC
CATTTGTGTCTGATTTGATTTTACTGTTTTTTCCCTGATTTTATGGAGTAGCATTGTGAC
CTGTTTTCCTTTGTCTTATATAACTTTAGTAAACTAACCACTGTCAATGATTGAGGGCAG
GTGGCACGTGGGGAAGAGGGGACTTGGCACGCAGTGGCTACCTGGGCATTTGTGGTCATT
TCAGTTTCCATCTCCCCAGCGGGGGCTCCCTGGGTGAAAGGCCACAGTATTTTGGGTTGG
TAGGCAAATTGCAACATTCTGGACATGGCCTGAGGAAGGCCTCTTCTTATAAGATTCTCA
GACCAAATTCTAGACCAAAGACACATGCAGACCAAGTCCCCAGGCCCCGCCTGGAAGGAA
GTCGTTCCTCAACTCTCCCCAAGGCACCTGTCTCCAATCAGAGCCCTCTCGCCCAGCCAG
CCCTGGCTCTGTGTGCAGAGCA
Protein sequence predicted based on nucleotide sequence
MATKEKLQCLKDFHKDILKPSPGKSPGTRPEDEAEGKPPQREKWSSKIDFVLSVAGGFVG LGNVWRFPYLCYKNGGGAFLIPYFIFLFGSGLPVFFLEIIIGQYTSEGGITCWEKICPLF SGIGYASVVIVSLLNVYYIVILAWATYYLFQSFQKELPWAHCNHSWNTPHCMEDTMRKNK SVWITISSTNFTSPVIEFWERNVLSLSPGIDHPGSLKWDLALCLLLVWLVCFFCIWKGVR STGKVVYFTATFPFAMLLVLLVRGLTLPGAGAGIKFYLYPDITRLEDPQVWIDAGTQIFF SYAICLGAMTSLGSYNKYKYNSYRDCMLLGCLNSGTSFVSGFAIFSILGFMAQEQGVDIA DVAESGPGLAFIAYPKAVTMMPLPTFWSILFFIMLLLLGLDSQFVEVEGQITSLVDLYPS FLRKGYRREIFIAFVCSISYLLGLTMVTEGGMYVFQLFDYYAASGVCLLWVAFFECFVIA WIYGGDNLYDGIEDMIGYRPGPWMKYSWAVITPVLCVGCFIFSLVKYVPLTYNKTYVYPN WAIGLGWSLALSSMLCVPLVIVIRLCQTEGPFLVRVKYLLTPREPNRWAVEREGATPYNS RTVMNGALVKPTHIIVETMM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
