DNASU Plasmid | PSMD5 (H. sapiens )<br>In pANT7_cGST (GST-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: PSMD5

Gene Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5

Map: pANT7_cGST.doc

Original Clone ID:

FLH224949.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pANT7_cGST

Format:

FUSION

Source:

HIP

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HsCD00078068

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1512nts

Open reading frame: 1 to 1512

Reference Sequence Alignment

ATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGAG
GAGCTACGCGCGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCAA
GCGGCGGAGCTGCGCCTCGGCCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAAG
ACTACTTTGTGTGTATCCATTCTGGAGAGATTGCTCCAAGCTATGGAACCGGTTCACGTG
GCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTAATTCACCCTGATGATTCTGTAAAA
ATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGATGCTGTTACTGAGATT
CTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAATCTATCT
GTAGCAAAAGCGGCTATCAAATCCCTGTCAAGAATATCACTAACCCAAGCTGGACTGGAG
GCTTTATTTGAAAGCAATCTGCTGGATGATTTGAAAAGTGTAATGAAAACAAATGACATT
GTTCGATACAGGGTGTATGAGCTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTTA
AACTACTGTACCACAAGTGGATTGGTAACCCAGCTCCTGAGAGAGCTGACTGGTGAGGAT
GTGTTGGTCAGAGCCACCTGTATAGAAATGGTGACATCACTGGCATATACTCATCATGGG
CGACAATATCTTGCTCAAGAAGGAGTAATTGACCAAATTTCTAATATAATTGTTGGGGCA
GATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTCGTGAAGTTTTTTGGAAACCTG
GCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTTGTGGAAAAAGTC
TTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTTGGA
ATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGAA
CGCTTGCTTATGAGAATAGGACATCAATCAAAGAATGCCCCAGTGGAGCTAAAAATTAGA
TGTTTGGATGCAATTTCATCTCTTCTGTACTTACCACCTGAGCAGCAGACTGATGACCTT
CTGAGGATGACAGAATCCTGGTTTTCTTCTTTATCTCGGGATCCACTGGAGCTCTTCCGT
GGCATTAGTAGTCAGCCCTTCCCTGAACTACACTGTGCTGCCTTAAAAGTGTTTACGGCC
ATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGTCCAGGTTTTGTAGAATAT
GTGGTGGACCGGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATATGAACTAGTG
AAAGCACTTGCCAATTCCAAGACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAGG
CTCAGAACTTACCTGAGTGAAGGGCCATACTATGTGAAACCTGTTTCCACGACAGCAGTA
GAAGGAGCCGAA

Protein sequence predicted based on nucleotide sequence

MAAQALALLREVARLEAPLEELRALHSVLQAVPLNELRQQAAELRLGPLFSLLNENHREK
TTLCVSILERLLQAMEPVHVARNLRVDLQRGLIHPDDSVKILTLSQIGRIVENSDAVTEI
LNNAELLKQIVYCIGGENLSVAKAAIKSLSRISLTQAGLEALFESNLLDDLKSVMKTNDI
VRYRVYELIIEISSVSPESLNYCTTSGLVTQLLRELTGEDVLVRATCIEMVTSLAYTHHG
RQYLAQEGVIDQISNIIVGADSDPFSSFYLPGFVKFFGNLAVMDSPQQICERYPIFVEKV
FEMIESQDPTMIGVAVDTVGILGSNVEGKQVLQKTGTRFERLLMRIGHQSKNAPVELKIR
CLDAISSLLYLPPEQQTDDLLRMTESWFSSLSRDPLELFRGISSQPFPELHCAALKVFTA
IANQPWAQKLMFNSPGFVEYVVDRSVEHDKASKDAKYELVKALANSKTIAEIFGNPNYLR
LRTYLSEGPYYVKPVSTTAVEGAE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Insert was fully sequenced in parent vector

Reference Sequence Annotations:

End on reference sequence 1516

Start on reference sequence 2

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pANT7_cGST

Synonyms:

pANT7-cGST

Sequencing Primer:

Forward: NAP150

Reverse: NAP138R

Description:

with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial

Comments:

One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems

Size (bp):

5964

Parent Vector:

None

Properties:

Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker

Author Name:

Joshua LaBaer
Al Lau
Niroshan Ramachandran

Publications:

PMID: 15232106

Title: Self-assembling protein microarrays

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 bacterial origin of replication

3931

3249

death cassette

ccdB

ccdB death cassette present in 'empty vector' form only (replace with gene of interest)

1876

2085

primer

NAP138

NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?

2405

2384

primer

NAP150

Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?

388

408

promoter

T7 transcriptional start sequence

5940

5964

recombination site

attR1

AttR1 site for recombination in empty vector

549

565

recombination site

attR2

AttR2 site for recombination in empty vector

2236

2251

selectable marker

CmR

chloramphenicol resistance

782

1438

selectable marker

AmpR

ampicillin resistance gene

4688

4029

tag

GST

Glutathione transferase (GST) tag

2282

2950

Gene

Gene Symbol :

PSMD5

Symbol Nomenclature :

PSMD5

SYNONYM :

S5B

Designation :

26S protease subunit S5 basic|26S proteasome non-ATPase regulatory subunit 5|26S proteasome subunit S5B

Full Nomenclature :

proteasome (prosome, macropain) 26S subunit, non-ATPase, 5

GENEID :

5711

Locus Tag :

RP11-27I1.5

HGNC :

9563

MIM :

604452

Vega :

OTTHUMG00000020573

Target GenBank:

BC014478

Reference Sequence Alignment

  

HsCD00078068        ATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGAG
NM_005047.3         ATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGAG
NM_001270427.1      ATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGAG
                    ************************************************************

HsCD00078068        GAGCTACGCGCGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCAA
NM_005047.3         GAGCTACGCGCGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCAA
NM_001270427.1      GAGCTACGCGCGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCAA
                    ************************************************************

HsCD00078068        GCGGCGGAGCTGCGCCTCGGCCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAAG
NM_005047.3         GCGGCGGAGCTGCGCCTCGGCCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAAG
NM_001270427.1      GCGGCGGAGCTGCGCCTCGGCCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAAG
                    ************************************************************

HsCD00078068        ACTACTTTGTGTGTATCCATTCTGGAGAGATTGCTCCAAGCTATGGAACCGGTTCACGTG
NM_005047.3         ACTACTTTGTGTGTATCCATTCTGGAGAGATTGCTCCAAGCTATGGAACCGGTTCACGTG
NM_001270427.1      ACTACTTTGTGTGTATCCATTCTGGAGAGATTGCTCCAAGCTATGGAACCGGTTCACGTG
                    ************************************************************

HsCD00078068        GCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTAATTCACCCTGATGATTCTGTAAAA
NM_005047.3         GCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTAATTCACCCTGATGATTCTGTAAAA
NM_001270427.1      GCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTAATTCACCCTGATGATTCTGTAAAA
                    ************************************************************

HsCD00078068        ATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGATGCTGTTACTGAGATT
NM_005047.3         ATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGATGCTGTTACTGAGATT
NM_001270427.1      ATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGATGCTGTTACTGAGATT
                    ************************************************************

HsCD00078068        CTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAATCTATCT
NM_005047.3         CTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAATCTATCT
NM_001270427.1      CTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAATCTATCT
                    ************************************************************

HsCD00078068        GTAGCAAAAGCGGCTATCAAATCCCTGTCAAGAATATCACTAACCCAAGCTGGACTGGAG
NM_005047.3         GTAGCAAAAGCGGCTATCAAATCCCTGTCAAGAATATCACTAACCCAAGCTGGACTGGAG
NM_001270427.1      GTAGCAAAAGCG------------------------------------------------
                    ************                                                

HsCD00078068        GCTTTATTTGAAAGCAATCTGCTGGATGATTTGAAAAGTGTAATGAAAACAAATGACATT
NM_005047.3         GCTTTATTTGAAAGCAATCTGCTGGATGATTTGAAAAGTGTAATGAAAACAAATGACATT
NM_001270427.1      ------------------------------------------------------------
                                                                                

HsCD00078068        GTTCGATACAGGGTGTATGAGCTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTTA
NM_005047.3         GTTCGATACAGGGTGTATGAGCTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTTA
NM_001270427.1      ---------------------CTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTTA
                                         ***************************************

HsCD00078068        AACTACTGTACCACAAGTGGATTGGTAACCCAGCTCCTGAGAGAGCTGACTGGTGAGGAT
NM_005047.3         AACTACTGTACCACAAGTGGATTGGTAACCCAGCTCCTGAGAGAGCTGACTGGTGAGGAT
NM_001270427.1      AACTACTGTACCACAAGTGGATTGGTAACCCAGCTCCTGAGAGAGCTGACTGGTGAGGAT
                    ************************************************************

HsCD00078068        GTGTTGGTCAGAGCCACCTGTATAGAAATGGTGACATCACTGGCATATACTCATCATGGG
NM_005047.3         GTGTTGGTCAGAGCCACCTGTATAGAAATGGTGACATCACTGGCATATACTCATCATGGG
NM_001270427.1      GTGTTGGTCAGAGCCACCTGTATAGAAATGGTGACATCACTGGCATATACTCATCATGGG
                    ************************************************************

HsCD00078068        CGACAATATCTTGCTCAAGAAGGAGTAATTGACCAAATTTCTAATATAATTGTTGGGGCA
NM_005047.3         CGACAATATCTTGCTCAAGAAGGAGTAATTGACCAAATTTCTAATATAATTGTTGGGGCA
NM_001270427.1      CGACAATATCTTGCTCAAGAAGGAGTAATTGACCAAATTTCTAATATAATTGTTGGGGCA
                    ************************************************************

HsCD00078068        GATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTCGTGAAGTTTTTTGGAAACCTG
NM_005047.3         GATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTCGTGAAGTTTTTTGGAAACCTG
NM_001270427.1      GATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTCGTGAAGTTTTTTGGAAACCTG
                    ************************************************************

HsCD00078068        GCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTTGTGGAAAAAGTC
NM_005047.3         GCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTTGTGGAAAAAGTC
NM_001270427.1      GCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTTGTGGAAAAAGTC
                    ************************************************************

HsCD00078068        TTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTTGGA
NM_005047.3         TTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTTGGA
NM_001270427.1      TTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTTGGA
                    ************************************************************

HsCD00078068        ATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGAA
NM_005047.3         ATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGAA
NM_001270427.1      ATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGAA
                    ************************************************************

HsCD00078068        CGCTTGCTTATGAGAATAGGACATCAATCAAAGAATGCCCCAGTGGAGCTAAAAATTAGA
NM_005047.3         CGCTTGCTTATGAGAATAGGACATCAATCAAAGAATGCCCCAGTGGAGCTAAAAATTAGA
NM_001270427.1      CGCTTGCTTATGAGAATAGGACATCAATCAAAGAATGCCCCAGTGGAGCTAAAAATTAGA
                    ************************************************************

HsCD00078068        TGTTTGGATGCAATTTCATCTCTTCTGTACTTACCACCTGAGCAGCAGACTGATGACCTT
NM_005047.3         TGTTTGGATGCAATTTCATCTCTTCTGTACTTACCACCTGAGCAGCAGACTGATGACCTT
NM_001270427.1      TGTTTGGATGCAATTTCATCTCTTCTGTACTTACCACCTGAGCAGCAGACTGATGACCTT
                    ************************************************************

HsCD00078068        CTGAGGATGACAGAATCCTGGTTTTCTTCTTTATCTCGGGATCCACTGGAGCTCTTCCGT
NM_005047.3         CTGAGGATGACAGAATCCTGGTTTTCTTCTTTATCTCGGGATCCACTGGAGCTCTTCCGT
NM_001270427.1      CTGAGGATGACAGAATCCTGGTTTTCTTCTTTATCTCGGGATCCACTGGAGCTCTTCCGT
                    ************************************************************

HsCD00078068        GGCATTAGTAGTCAGCCCTTCCCTGAACTACACTGTGCTGCCTTAAAAGTGTTTACGGCC
NM_005047.3         GGCATTAGTAGTCAGCCCTTCCCTGAACTACACTGTGCTGCCTTAAAAGTGTTTACGGCC
NM_001270427.1      GGCATTAGTAGTCAGCCCTTCCCTGAACTACACTGTGCTGCCTTAAAAGTGTTTACGGCC
                    ************************************************************

HsCD00078068        ATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGTCCAGGTTTTGTAGAATAT
NM_005047.3         ATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGTCCAGGTTTTGTAGAATAT
NM_001270427.1      ATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGTCCAGGTTTTGTAGAATAT
                    ************************************************************

HsCD00078068        GTGGTGGACCGGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATATGAACTAGTG
NM_005047.3         GTGGTGGACCGGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATATGAACTAGTG
NM_001270427.1      GTGGTGGACCGGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATATGAACTAGTG
                    ************************************************************

HsCD00078068        AAAGCACTTGCCAATTCCAAGACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAGG
NM_005047.3         AAAGCACTTGCCAATTCCAAGACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAGG
NM_001270427.1      AAAGCACTTGCCAATTCCAAGACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAGG
                    ************************************************************

HsCD00078068        CTCAGAACTTACCTGAGTGAAGGGCCATACTATGTGAAACCTGTTTCCACGACAGCAGTA
NM_005047.3         CTCAGAACTTACCTGAGTGAAGGGCCATACTATGTGAAACCTGTTTCCACGACAGCAGTA
NM_001270427.1      CTCAGAACTTACCTGAGTGAAGGGCCATACTATGTGAAACCTGTTTCCACGACAGCAGTA
                    ************************************************************

HsCD00078068        GAAGGAGCCGAATTG
NM_005047.3         GAAGGAGCCGAATGA
NM_001270427.1      GAAGGAGCCGAATGA
                    ************* .

Pathways

Reactome :

Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

Activation of NF-kappaB in B cells

Reactome :

Adaptive Immune System

Reactome :

Antigen processing-Cross presentation

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

APC/C:Cdc20 mediated degradation of Securin

Reactome :

APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1

Reactome :

Apoptosis

Reactome :

Assembly of the pre-replicative complex

Reactome :

Asymmetric localization of PCP proteins

Reactome :

AUF1 (hnRNP D0) destabilizes mRNA

Reactome :

Autodegradation of Cdh1 by Cdh1:APC/C

Reactome :

Autodegradation of the E3 ubiquitin ligase COP1

Reactome :

beta-catenin independent WNT signaling

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

CDK-mediated phosphorylation and removal of Cdc6

Reactome :

CDT1 association with the CDC6:ORC:origin complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Cross-presentation of soluble exogenous antigens (endosomes)

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

degradation of AXIN

Reactome :

degradation of DVL

Reactome :

Disease

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

ER-Phagosome pathway

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

G1/S Transition

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

M Phase

Reactome :

M/G1 Transition

Reactome :

Metabolism

Reactome :

Metabolism of amino acids and derivatives

Reactome :

Metabolism of mRNA

Reactome :

Metabolism of RNA

Reactome :

Mitotic Anaphase

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Orc1 removal from chromatin

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

p53-Independent DNA Damage Response

Reactome :

p53-Independent G1/S DNA damage checkpoint

Reactome :

PCP/CE pathway

Reactome :

Regulation of activated PAK-2p34 by proteasome mediated degradation

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of Apoptosis

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Regulation of mRNA stability by proteins that bind AU-rich elements

Reactome :

Regulation of ornithine decarboxylase (ODC)

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

SCF-beta-TrCP mediated degradation of Emi1

Reactome :

Separation of Sister Chromatids

Reactome :

Signal Transduction

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signaling by Wnt

Reactome :

Stabilization of p53

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

Reactome :

TCF dependent signaling in response to WNT

Reactome :

Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Reactome :

Ubiquitin-dependent degradation of Cyclin D

Reactome :

Ubiquitin-dependent degradation of Cyclin D1

Reactome :

Vif-mediated degradation of APOBEC3G

Reactome :

Vpu mediated degradation of CD4

WikiPathway :

Proteasome Degradation

Protein

UniProt :

Q16401

HPRD :

05121

Clone

Cloning Information :

224949

HIP Master Clone ID :

159106

Original Clone ID :

FLH224949.01L

Organism

Species Specific ID:

5711

Genome

Chromosome :

Map Location :

9q33.2

Ensembl :

ENSG00000095261

Reagents

Labome :

PSMD5-antibody

PSMD5 (H. sapiens )In pANT7_cGST (GST-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

PSMD5 (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)