Gene:
Gene Symbol:
DENND4A
Gene Name:
DENN/MADD domain containing 4A
Original Clone ID:
FLH258728.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00080045
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATTGAAGACAAGGGGCCTCGTGTTGCTGACTACTTTGTTGTAGCAGGATTAACTGAT
GTTTCAAAGCCTCTAGAAGAAGAAATTCACTTCAATGATGCTTGTCATAAAGTAGCTAAA
CCAAAAGAACCTATTACAGATGTTTCAGTTATTATCAAATCTCTTGGGGAGGAAGTCCCA
CAGGATTATATCTGTATTGATGTTACCCCAACTGGATTGTCAGCTGATCTTAATAATGGA
AGTCTTGTGGGGCCACAGATTTACCTTTGCTATAGAAGAGGAAGAGATAAGCCTCCACTT
ACAGATCTGGGGGTTTTATATGACTGGAAAGAAAGATTGAAACAGGGTTGTGAAATTATT
CAGAGTACTCCCTATGGGCGCCCCGCAAATATTAGTGGGAGTACCTCATCACAAAGAATT
TATATCACTTACCGAAGAGCCTCTGAAAACATGACTCAGAATACGTTGGCTGTCACTGAC
ATATGTATTATTATACCCAGTAAAGGAGAAAGCCCACCACACACGTTCTGCAAAGTCGAC
AAGAATCTCAATAACAGTATGTGGGGATCAGCAGTATACTTGTGTTATAAAAAATCGGTG
GCAAAGACGAACACTGTATCTTACAAAGCTGGCCTAATTTGTAGATATCCTCAAGAAGAT
TATGAGTCATTCTCACTACCGGAATCTGTTCCTCTATTTTGTTTACCAATGGGTGCAACC
ATTGAATGTTGGCCATCAAATAGCAAATACCCTCTGCCTGTATTTTCTACTTTTGTTTTA
ACTGGAGCCTCAGCTGAAAAGGTTTATGGTGCTGCTATTCAGTTTTATGAACCATACTCT
GAGGAGAATCCCACAGAAAAGCAGAGACTTCTTTTGGGTTTAACATCAGCAGATGGGAAG
TCTGATAGTTCCAAAACAATTCATACTAACAAATGCATCTGTCTTCTTTCTCACTGGCCT
TTTTTTGATGCATTCAGGAAGTTTCTGACTTTTCTGTATCGTTATTCCATCTCTGGGCCT
CATGTCCTTCCAATTGAGAAGCATATTTCTCATTTTATGCATAAAGTTCCTTTTCCATCT
CCTCAGAGACCACGGATTTTAGTTCAGTTATCACCACATGATAATCTGATTCTCAGTCAG
CCTGTGTCTTCACCTCTTCCACTAAGTGGTGGCAAGTTTTCAACACTACTGCAGAATTTA
GGCCCTGAAAATGCTGTGACACTACTGGTGTTTGCAGTAACAGAACATAAAATTCTTATC
CATTCCCTACGGCCATCCGTGCTTACTAGTGTGACAGAAGCATTAGTGTCTATGATTTTC
CCTTTCCACTGGCCATGCCCGTATGTTCCTCTCTGCCCACTGGCTTTAGCAGATGTCTTG
AGTGCACCATGTCCATTCATAGTAGGGATTGATTCAAGATACTTTGATCTCTATGATCCT
CCACCAGATGTCAGTTGTGTTGATGTAGATACCAACACTATTTCTCAAATTGGTGACAAG
AAAAATGTAGCCTGGAAGATACTTCCAAAGAAGCCATGTAAAAATCTGATGAACACACTG
AATAATTTGCATCAGCAATTGGCAAAATTGCAGCAGAGACCGAGAGATGATGGACTCATG
GATTTAGCAATAAATGACTATGATTTTAATTCTGGAAAGAGGTTGCACATGATAGATTTG
GAAATCCAAGAGGCATTTTTGTTTTTCATGGCCTCTATTTTAAAAGGTTACAGATCATAC
TTAAGGCCAATAACACAAGCCCCATCTGAGACAGCCACAGATGCAGCCTCTCTCTTTGCC
CTACAAGCTTTCTTAAGAAGCCGGGACCGGTCACATCAAAAATTCTATAACATGATGACC
AAAACACAAATGTTTATTCGCTTCATTGAAGAATGTTCTTTTGTTAGTGACAAAGATGCA
AGCCTGGCATTTTTTGATGATTGTGTAGATAAAGTGGATATGGACAAGAGCGGTGAAGTA
CGACTTATAGAACTGGATGAATCTTTCAAAAGTGAACACACTGTTTTTGTAACACCACCT
GAGATCCCCCACCTACCCAATGGTGAAGAACCCCCTTTACAGTACAGCTATAATGGATTT
CCAGTTCTTAGGAACAATTTATTTGAAAGACCTGAAGGATTTCTACAAGCAAAGAAGAAC
AAATTGCCTTCAAAATCAAGTAGTCCTAATAGCCCTTTGCCCATGTTCAGAAGAACAAAA
CAGGAAATTAAATCAGCCCATAAAATTGCAAAGAGGTACTCTTCCATCCCTCAGATGTGG
TCTAGGTGTCTACTGCGCCACTGTTATGGACTGTGGTTTATTTGTCTCCCAGCTTATGTG
AAAGTCTGTCATTCAAAAGTCAGGGCTCTGAAAACAGCATATGATGTGCTTAAAAAAATG
CAGTCAAAGAAGATGGATCCACCTGATGAGGTATGCTACCGCATTCTTATGCAACTCTGT
GGACAATATGATCAGCCAGTGCTTGCAGTTCGAGTGCTTTTTGAAATGCAGAAAGCTGGT
ATTGACCCCAATGCCATTACTTATGGTTATTATAATAAGGCTGTTTTGGAAAGTACCTGG
CCTTCAAGAAGTCGTAGTGGCTATTTTCTTTGGACAAAAGTAAGAAATGTTGTTTTAGGA
GTAACACAGTTCAAAAGAGCTTTAAAGAAGCATGCACACTTATCACAAACAACTCTCTCA
GGTGGCCAGTCTGATCTTGGATATAATTCATTATCTAAAGATGAAGTTAGAAGAGGGGAT
ACATCTACTGAAGACATTCAAGAAGAAAAAGATAAAAAAGGGAGTGATTGTAGTTCCTTG
TCAGAGAGTGAGAGTACAAAAGGAAGTGCTGATTGCCTTCCTAAGCTCAGTTATCAAAAT
TCTTCCAGTATCGTTCGCCTCACTGGTACAAGTAACAACAGTGCTGGTAAAATATCAGGA
GAAAGCATGGAAAGCACACCTGAGCTGCTCTTAATATCATCTCTTGAAGATACAAATGAA
ACACGAAACATTCAAAGTAGATGCTTCAGGAAAAGACATAAAAGTGACAATGAAACTAAT
TTGCAGCAGCAAGTGGTCTGGGGAAATAGAAACCGTAATCTTAGTGGAGGGGTACTGATG
GGATTTATGCTCAATAGAATTAACCAGGAAGCAACCCCTGGAGATATAGTTGAAAAATTG
GGAGCTGATGCAAAAATTCTTTCAAATGTTATCTCAAAAAGCACGAGACCAAATACTCTT
GATATTGGGAAGCCACCTTTAAGATCAAAAAGAGACAGTCTAGAAAAGGAATCTAGTGAT
GATGATACACCTTTTGATGGTTCTAACTATTTGGCAGATAAAGTGGATTCTCCTGTTATT
TTTGACTTAGAAGACTTAGACAGTGAAACAGATGTATCAAAAGCAGGATGTGTTGCTACA
CAAAATCCCAAGAGGATTCAACGTATGAACAGCAGCTTTTCAGTAAAACCTTTTGAAAAA
ACTGATGTCGCAACAGGATTTGATCCCCTCTCTCTTTTGGTTGCTGAGACTGAACAGCAG
CAAAAAGAAGAAGAGGAGGAGGATGAAGATGATAGCAAAAGCATTTCAACACCATCTGCT
AGGCGTGATTTAGCTGAAGAAATTGTGATGTATATGAATAACATGAGCAGTCCTTTGACA
AGTCGTACACCAAGCATTGATTTACAACGGGCATGTGATGATAAATTAAATAAGAAAAGT
CCACCATTGGTCAAGGCATGCAGAAGATCTAGTCTGCCTCCTAATTCTCCTAAGCCAGTG
AGACTTACCAAATCTAAAAGTTACACAAAAAGTGAGGAGAAACCAAGGGATAGACTTTGG
TCTTCTCCAGCCTTCTCACCTACTTGCCCATTTAGGGAAGAATCTCAGGATACATTAACC
CACTCATCACCTTCATTTAACTTAGATACACTACTAGTACCTAAACTAGATGTTCTAAGA
AACAGTATGTTCACTGCTGGAAAAGGAGTTGCAGAGAAAGCAAGCAAATGGTATTCAAGA
TTCACCATGTACACCACATCATCTAAGGATCAAAGTTCTGATCGTACCAGTCTTTCTTCA
GTGGGAGCCCAGGATTCTGAATCTACCTCTTTAACAGATGAAGATGTCTGCCATGAGTTG
GAAGGACCTATCTCCTCTCAAGAGACCAGTGCTACTTCAGGGACTAAGAGAATTGATCTC
AGCCGAATAAGCCTGGAAAGTTCTGCATCCTTGGAAGGATCTCTGTCGAAGTTTGCCTTA
CCTGGGAAATCAGAAGTGACATCTTCCTTCAACGCGAGTAATACAAATATCTTCCAGAAC
TATGCAATGGAGGTTCTCATCTCAAGTTGCTCTCGGTGTAGAACTTGTGATTGTCTTGTC
CATGATGAGGAAATCATGGCTGGCTGGACAGCAGATGATTCAAATCTCAATACTACATGC
CCATTCTGTGGCAATATCTTCTTACCCTTTCTGAATATAGAAATAAGAGATTTAAGACGA
CCTGGAAGATACTTTCTAAAGTCAAGCCCATCAACAGAAAATATGCACTTTCCATCCTCC
ATTTCAAGTCAGACGAGGCAGTCTTGCATTTCAACATCAGCCTCTGGTCTTGACACATCT
GCTCTCTCTGTTCAAGGGAATTTTGATCTAAATAGCAAATCTAAACTGCAGGAAAATTTT
TGCACCCGAAGTATTCAGATCCCTGCTAATAGATCAAAAACAGCTATGTCTAAATGTCCA
ATATTTCCAATGGCCAGGAGTATTAGTACTTCTGGCCCCTTGGATAAGGAAGATACTGGA
AGACAGAAGCTCATTTCTACAGGCAGCCTGCCAGCTACTTTACAAGGAGCTACAGATTCT
TTAGGTTTAGAATGGCACCTTCCAAGTCCCGATCCTGTCACTGTTCCGTATCTTAGTCCT
TTAGTGGTATGGAAAGAACTTGAAAGCTTATTGGAAAATGAAGGTGATCATGCAATAACA
GTAGCAGACTTTGTGGACCATCATCCAATTGTTTTTTGGAACCTGGTATGGTATTTCAGA
CGTCTTGACTTGCCCAGTAACTTACCTGGATTGATTCTTTCTTCTGAGCACTGTAACAAG
TATTCAAAGATTCCCCGCCACTGTATGTCTGAAGATAGCAAATATGTTTTAATACAAATG
TTATGGGACAATATGAAGTTACATCAGGATCCGGGACAGCCCTTGTACATTCTCTGGAAT
GCACACACCCAAAAATATCCAATGGTCCATTTATTGCAAAAAAGTGATAACTCATTTAAC
CAGGAACTGTTGAAAAGTATGGTAAAAAGCATTAAAATGAATGATGTCTATGGACCAATG
AGTCAGATTTTAGAGACACTGAATAAGTGTCCACATTTTAAAAGACAGAGGAGTTTATAC
AGAGAAATACTATTTCTCTCACTTGTGGCACTGGGAAGAGAAAACATTGATATAGATGCT
TTTGATAAAGAGTACAAGATGGCATACGATCGTCTCACACCTAGTCAAGTCAAGAGTACA
CACAACTGTGATAGACCACCAAGTACAGGGGTGATGGAATGTCGAAAAACCTTTGGAGAA
CCTTATCTTTTG
Protein sequence predicted based on nucleotide sequence
MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVP QDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEII QSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVD KNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGAT IECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENPTEKQRLLLGLTSADGK SDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPS PQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILI HSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDP PPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLM DLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFA LQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEV RLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKN KLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYV KVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAG IDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLS GGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQN SSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETN LQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTL DIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVAT QNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSA RRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPV RLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLR NSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHEL EGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQN YAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRR PGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENF CTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDS LGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFR RLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWN AHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLY REILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGE PYLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
210
End on reference sequence
5801
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
