Gene:
Gene Symbol:
ZNF471
Gene Name:
zinc finger protein 471
Original Clone ID:
FLH265117.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR/D-TOPO
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080496
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATGTTGAAGTAGTAAAAGTCATGCCCCAGGACTTAGTGACATTCAAGGATGTGGCA
ATAGATTTTTCCCAGGAAGAATGGCAATGGATGAACCCTGCTCAGAAGCGTTTATACAGG
AGTATGATGTTGGAGAACTATCAGAGCCTGGTATCACTTGGTCTTTGCATTTCTAAGCCA
TGTGTGATCTCCTTATTGGAGCAAGGGAGAGAGCCTTGGGAGATGACGAGTGAGATGACA
AGAAGCCCATTCTCAGATTGGGAATCTATATATGTGACACAGGAATTACCTCTGAAGCAG
TTCATGTATGATGATGCATGCATGGAGGGAATTACTAGCTATGGACTTGAGTGTTCCACT
TTTGAAGAAAATTGGAAATGGGAAGACCTTTTTGAGAAGCAGATGGGAAGTCATGAGATG
TTTAGCAAGAAAGAAATAATCACTCATAAAGAAACCATCACTAAGGAAACAGAATTCAAA
TATACTAAATTTGGGAAATGTATCCATCTGGAAAACATAGAAGAGAGTATTTATAATCAC
ACATCAGATAAAAAAAGCTTCTCCAAAAATTCTATGGTAATAAAACACAAGAAAGTCTAT
GTAGGAAAGAAGCTTTTTAAATGTAATGAATGTGACAAAACCTTCACCCATAGCTCATCC
CTTACTGTTCATTTTAGAATTCATACTGGTGAAAAACCATATGCATGTGAGGAATGTGGA
AAAGCCTTCAAGCAAAGGCAACACCTTGCTCAACATCACAGAACACATACTGGAGAGAAA
CTCTTTGAATGTAAAGAATGTAGGAAAGCCTTCAAACAAAGTGAACACCTTATTCAGCAT
CAAAGAATTCATACTGGAGAAAAACCATATAAATGTAAGGAATGCAGAAAAGCCTTCAGA
CAGCCTGCACACCTTGCTCAGCATCAGAGAATTCATACTGGAGAGAAACCCTATGAATGT
AAAGAATGTGGCAAAGCCTTCAGTGATGGCTCGTCTTTTGCTCGACATCAGAGATGTCAC
ACTGGCAAAAGACCCTATGAATGTATTGAGTGTGGGAAGGCTTTTAGGTATAACACATCT
TTTATTCGTCACTGGAGGAGTTATCATACTGGAGAGAAGCCTTTTAATTGCATTGATTGT
GGGAAAGCCTTCAGTGTTCACATAGGACTTATTCTGCATAGGAGAATTCATACAGGAGAG
AAACCTTACAAATGTGGTGCGTGTGGAAAAACCTTCAGCTCGGGTTCATCCCGTACTGTA
CATCAGAGAATTCATACAGGAGAGAAACCTTATGAATGTGATATATGTGGGAAAGATTTT
AGCCATCATGCATCACTCACTCAGCATCAAAGAGTACATTCTGGAGAGAAACCGTATGAA
TGCAAGGAATGTGGGAAAGCCTTTAGGCAGAATGTACACCTTGTTAGTCATTTGAGAATT
CATACTGGTGAAAAACCCTATGAATGTAAAGAATGTGGAAAAGCTTTTAGAATCAGTTCA
CAGCTGGCTACTCATCAGAGAATTCATACTGGAGAGAAGCCTTATGAATGTATTGAATGT
GGAAATGCTTTCAAACAGAGATCACACCTTGCCCAACATCAGAAAACTCATACAGGAGAG
AAACCTTATGAGTGTAATGAATGCGGGAAAGCCTTCAGCCAAACTTCCAATCTTACTCAA
CATCAAAGAATTCATACTGGAGAGAAACCCTATAAATGTACTGAATGTGGAAAGGCTTTT
AGTGATAGCTCATCCTGTGCTCAGCATCAAAGACTCCACACTGGCCAAAGGCCCTATCAG
TGTTTTGAATGTGGGAAGGCGTTCAGAAGAAAGTTATCCTTAATTTGTCATCAAAGAAGT
CATACTGGAGAAGAACCT
Protein sequence predicted based on nucleotide sequence
MNVEVVKVMPQDLVTFKDVAIDFSQEEWQWMNPAQKRLYRSMMLENYQSLVSLGLCISKP CVISLLEQGREPWEMTSEMTRSPFSDWESIYVTQELPLKQFMYDDACMEGITSYGLECST FEENWKWEDLFEKQMGSHEMFSKKEIITHKETITKETEFKYTKFGKCIHLENIEESIYNH TSDKKSFSKNSMVIKHKKVYVGKKLFKCNECDKTFTHSSSLTVHFRIHTGEKPYACEECG KAFKQRQHLAQHHRTHTGEKLFECKECRKAFKQSEHLIQHQRIHTGEKPYKCKECRKAFR QPAHLAQHQRIHTGEKPYECKECGKAFSDGSSFARHQRCHTGKRPYECIECGKAFRYNTS FIRHWRSYHTGEKPFNCIDCGKAFSVHIGLILHRRIHTGEKPYKCGACGKTFSSGSSRTV HQRIHTGEKPYECDICGKDFSHHASLTQHQRVHSGEKPYECKECGKAFRQNVHLVSHLRI HTGEKPYECKECGKAFRISSQLATHQRIHTGEKPYECIECGNAFKQRSHLAQHQKTHTGE KPYECNECGKAFSQTSNLTQHQRIHTGEKPYKCTECGKAFSDSSSCAQHQRLHTGQRPYQ CFECGKAFRRKLSLICHQRSHTGEEP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
134
End on reference sequence
2014
Mutations:
None
Discrepancy:
t932g,I311S
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR/D-TOPO
Synonyms:
pENTR-D-TOPO
Description:
Gateway type (lambda att-type) recombinational cloning system entry (master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.
Size (bp):
2580
Parent Vector:
None
Properties:
Gateway, TOPO Cloning, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (NotI and AscI sites flank the TOPO cloning site)
680
693
TOPO recognition site
TOPO 1
TOPO recognition site 1
680
684
TOPO recognition site
TOPO 2
TOPO recognition site 2
689
693
bacterial origin
pUC ori
pUC-type origin of replication
1904
2577
primer site
M13 F
M13 forward priming site
537
552
primer site
M13 Rev
M13 reverse primer site
845
861
promoter
T7
T7promoter and primer binding site
821
840
recombination site
attL1
attL1 recombination site
569
668
recombination site
attL2
attL2 recombination site
705
804
selectable marker
KanR
kanamycin resistance gene
974
1783
trxn termination sequence
rrnB trxn term
rrnB T2 transcription termination sequence
268
295
trxn termination sequence
rrnB txn term
rrnB T1 transcription termination sequence
427
470
