Gene:
Gene Symbol:
Adam19
Gene Name:
a disintegrin and metallopeptidase domain 19 (meltrin beta)
Original Clone ID:
FLH265343.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080721
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2760nts
Open reading frame: 1 to 2760
ATGCCCGGGCGCGCGGGCGTCGCCCGGTTCTGCTTGCTGGCTCTCGCTCTGCAGCTACAT
TGGCCGCTGGCGGCGTGCGAGCCGGGATGGACCACAAGAGGAAGCCAAGAAGGTAGCCCT
CCGCTACAGCATGAACTCATAATACCTCAGTGGCGGACTTCAGAAAGCCCTGGGAGAGGA
AAGCATCCACTCAGAGCAGAGCTCAGGGTCATGGCTGAAGGGCGAGAGCTGATCCTAGAC
CTGGAGAAGAACGAGCACCTTTTTGCTCCAGCCTACACAGAAACCTGCTACACTGCAAGT
GGCAATCCTCAAACCAGCACGCTGAAGTCTGAGGATCACTGCTTTTACCACGGGACTGTG
AGGGACGTGGATGAGTCCAGTGTCACGCTCAGCACCTGCCGGGGAATTAGAGGACTGATT
ATAGTGAGAAGTAACCTCAGCTACATCATCGAGCCCGTCCCTAACAGCGACAGCCAACAC
CGTATTTACAGATCCGAACATCTCACGCTGCCCCCGGGGAACTGTGGGTTCGAGCACTCC
GGGCCCACCTCGAAGGACTGGGCCCTTCAGTTTACACATCAGACCAAAAAGCAACCTCGC
AGAATGAAACGGGAAGATCTACACTCTATGAAGTACGTGGAGCTTTACCTGGTGGCTGAT
TATGCAGAGTTTCAGAAGAATCGACATGACCAGGATGCCACCAAACGCAAGCTCATGGAG
ATTGCCAACTATGTTGATAAGTTTTACCGCTCCCTGAACATCCGAATTGCACTTGTCGGC
TTGGAGGTGTGGACGCATGGGGATAAGTGTGAAGTTTCAGAGAATCCCTACTCTACCCTC
TGGTCCTTTCTTAGTTGGAGGCGCAAGCTGCTTGCTCAGAAGAGCCATGACAATGCTCAG
CTAATCACGGGCAGGTCCTTCCAAGGCACCACCATTGGCCTGGCCCCCCTCATGGCCATG
TGCTCCGTGTACCAGTCTGGAGGAGTTAGCATGGACCACTCCGAGAATGCCATTGGTGTA
GCCTCCACTGTGGCCCATGAGATTGGCCACAACTTTGGCATGAGCCATGATTCTGCACAC
TGCTGTTCTGCCAGTGCAGCCGATGGCGGCTGCATCATGGCCGCCGCCACCGGGCACCCT
TTCCCCAAAGTGTTCAGTTGGTGTAACAGGAAGGAGCTGGACAGGTATCTGCAGACAGGA
GGAGGGATGTGTCTCTCCAACATGCCGGACACTAGGACGCTGTATGGAGGCCGGAGGTGT
GGCAACGGGTACCTGGAAGACGGTGAAGAATGTGACTGTGGAGAAGAGGAGGAATGTAAG
AACCCTTGCTGCAATGCCTCCAACTGCACTCTGAAGGAAGGGGCAGAGTGTGCCCATGGT
TCCTGCTGCCACCAGTGCAAGCTGGTGGCTCCTGGAACCCAGTGTCGGGAGCAGGTTCGG
CAATGTGACCTCCCCGAGTTCTGCACCGGCAAGTCTCCCCACTGCCCCACCAACTATTAT
CAGATGGATGGCACCCCCTGCGAGGGTGGCCAGGCCTACTGCTACAACGGCATGTGCCTC
ACTTACCAGGAACAGTGCCAGCAGCTGTGGGGACCTGGAGCCCGGCCTGCCCTCGATCTT
TGCTTTGAGAGGGTGAATGCTGCTGGTGACACCTATGGAAACTGTGGCAAGGGCTTGAAT
GGCCAATACAGGAAGTGCAGTCCCAGGGATGCCAAGTGTGGGAAGATTCAGTGCCAGAGC
ACCCAGGCCCGGCCCCTGGAATCCAACGCAGTATCTATTGACACCACCATCACCTTGAAC
GGGAGGCGGATCCACTGTCGGGGCACCCACGTCTACCGGGGTCCTGAGGAGGAGGAAGGG
GAAGGTGACATGCTGGACCCAGGGCTGGTGATGACTGGAACCAAGTGTGGCCACAACCAT
ATTTGCTTCGAGGGGCAGTGCAGGAACACCTCCTTCTTTGAGACGGAAGGCTGTGGGAAA
AAGTGCAATGGCCACGGGGTCTGCAACAACAACAAGAACTGTCATTGCTTCCCTGGCTGG
TCTCCACCTTTCTGTAACACCCCGGGAGATGGTGGCAGCGTCGACAGTGGTCCTTTGCCC
CCTAAGAGTGTGGGTCCCGTGATCGCTGGGGTGTTTTCAGCTCTCTTCGTGTTGGCAGTT
CTGGTGCTACTGTGTCACTGCTACAGACAGAGCCACAAACTGGGCAAACCCTCGGCTCTC
CCTTTCAAGCTGCGGCATCAGTTCAGTTGTCCCTTCAGGGTATCTCAGAGTGGTGGAACT
GGCCATGCCAACCCAACTTTCAAGTTGCAGACCCCCCAGGGCAAGCGAAAGGTGACTAAC
ACCCCTGAATCCCTCCGGAAGCCGTCCCACCCCCCTCCCCGGCCCCCTCCAGACTACCTG
CGCGTTGAATCGCCACCTGCACCATTGCCGGCACATCTGAACAGGGCTGCTGGGAGCTCC
CCAGAAGCTGGGGCTCGAATAGAAAGAAAGGAGTCAGCCAGGAGGCCTCCCCCAAGCCGA
CCCATGCCCCCTGCACCTAACTGCCTACTGTCCCAGGACTTCTCCAGGCCTCGACCACCT
CAGAAGGCACTCCCAGCCAATCCGGTGCCAGGCCAAAGGACCGGTCCCAGGTCAGGAGGC
ACCTCCCTGCTTCAGCCCCCTACTTCTGGTCCTCAGCCCCCCAGGCCTCCAGCAGTGCCT
GTTCCAAAGCTACCCGAGTACCGATCACAGAGGGTTGGAGCAATAATTAGCTCCAAGATC
Protein sequence predicted based on nucleotide sequence
MPGRAGVARFCLLALALQLHWPLAACEPGWTTRGSQEGSPPLQHELIIPQWRTSESPGRG KHPLRAELRVMAEGRELILDLEKNEHLFAPAYTETCYTASGNPQTSTLKSEDHCFYHGTV RDVDESSVTLSTCRGIRGLIIVRSNLSYIIEPVPNSDSQHRIYRSEHLTLPPGNCGFEHS GPTSKDWALQFTHQTKKQPRRMKREDLHSMKYVELYLVADYAEFQKNRHDQDATKRKLME IANYVDKFYRSLNIRIALVGLEVWTHGDKCEVSENPYSTLWSFLSWRRKLLAQKSHDNAQ LITGRSFQGTTIGLAPLMAMCSVYQSGGVSMDHSENAIGVASTVAHEIGHNFGMSHDSAH CCSASAADGGCIMAAATGHPFPKVFSWCNRKELDRYLQTGGGMCLSNMPDTRTLYGGRRC GNGYLEDGEECDCGEEEECKNPCCNASNCTLKEGAECAHGSCCHQCKLVAPGTQCREQVR QCDLPEFCTGKSPHCPTNYYQMDGTPCEGGQAYCYNGMCLTYQEQCQQLWGPGARPALDL CFERVNAAGDTYGNCGKGLNGQYRKCSPRDAKCGKIQCQSTQARPLESNAVSIDTTITLN GRRIHCRGTHVYRGPEEEEGEGDMLDPGLVMTGTKCGHNHICFEGQCRNTSFFETEGCGK KCNGHGVCNNNKNCHCFPGWSPPFCNTPGDGGSVDSGPLPPKSVGPVIAGVFSALFVLAV LVLLCHCYRQSHKLGKPSALPFKLRHQFSCPFRVSQSGGTGHANPTFKLQTPQGKRKVTN TPESLRKPSHPPPRPPPDYLRVESPPAPLPAHLNRAAGSSPEAGARIERKESARRPPPSR PMPPAPNCLLSQDFSRPRPPQKALPANPVPGQRTGPRSGGTSLLQPPTSGPQPPRPPAVP VPKLPEYRSQRVGAIISSKI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
128
End on reference sequence
2890
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
