Gene:
Gene Symbol:
Scube3
Gene Name:
signal peptide, CUB domain, EGF-like 3
Original Clone ID:
FLH265350.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080728
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2979nts
Open reading frame: 1 to 2979
ATGGGCTCGGGGCGCGTCCCCGGGCTCTGCCTGCTCCTCCTGCTGGTCCACGCGCGCGCC
GCCCAGCATGGCAAAGCTGCGCAAGATGTAGATGAGTGTGTGGAAGGCACGGACAATTGC
CACATCGATGCCATCTGCCAGAATACCCCACGGTCTTACAAGTGCATCTGCAAGTCCGGC
TACACGGGGGACGGGAAGCACTGCAAAGACGTGGATGAGTGTGAACGGGAGGACAACGCA
GGCTGTGTGCACGACTGTGTCAACATCCCTGGTAATTACCGGTGTACCTGCTACGACGGA
TTTCACCTGGCACATGACGGACACAACTGTCTGGATGTGGATGAGTGTGCAGAGGGCAAC
GGTGGCTGCCAGCAGAGCTGTGTCAACATGATGGGCAGCTACGAGTGCCATTGCAGAGAT
GGCTTCTTCCTCAGTGACAACCAGCACACCTGTATCCAGCGGCCAGAAGAAGGAATGAAC
TGCATGAACAAGAACCACGGCTGTGCCCACATTTGCCGGGAGACTCCCAAGGGGGGCATT
GCCTGTGAATGCCGCCCTGGCTTCGAGCTCACCAAGAACCAACGGGACTGTAAATTGACC
TGCAACTATGGTAATGGCGGCTGCCAGCACACCTGCGATGACACGGAGCAGGGTCCTCGG
TGCGGCTGCCACGTCAAGTTTGTGCTCCACACCGACGGGAAGACATGCATTGAAACCTGT
GCTGTCAACAACGGAGGCTGCGACAGTAAGTGCCATGATGCAGCGACCGGAGTCCACTGC
AGCTGTCCTGTGGGTTTCATGCTGCAGCCAGACAGAAAGACCTGCAAAGATATAGACGAG
TGCCGCTTAAACAACGGGGGCTGTGACCACATCTGCCGCAACACAGTGGGCAGCTTCGAG
TGCAGCTGCAAGAAGGGCTACAAGCTTCTCATCAACGAAAGGAGCTGCCAGGATATAGAT
GAGTGCTCCTTCGACCGAACCTGTGACCACATGTGTGTCAACACCCCGGGGAGCTTCCAG
TGTCTCTGTCATCGTGGCTACCTTCTATATGGTGTCACTCACTGCGGGGATGTGGACGAA
TGTAGCATCAATAAGGGAGGCTGCCGCTTTGGCTGCATCAACACCCCAGGCAGCTATCAG
TGTACCTGCCCAGCAGGCCAGGGCCGCCTTCACTGGAACGGGAAGGATTGCACAGAGCCA
GTGAAGTGTCAGAGCAGTCTTGGAGCCTCAAAAGCCATGCTTAGCTGCAATCGCTCGGGC
AAGAAGGATACTTGTGCCCTGACCTGTCCCTCCCGGGCCCGGTTCTTGCCAGAGTCAGAG
AACGGTTTCACCGTGAGCTGTGGTACCCCGAGCCCTAAAGCTGCTCCAGCCCGAGTGATC
CACTCTGGGAACAGCACCGTCTCCAGCTCCTGCCATGAGGCTGCAGTGCTGCCAGTCAAG
CAGAGAGCCTCCTTCAAGATCAAGGATGCCAAATGTCGCCTGCATCTGCGAAACAAAGGC
AAAGCCGAGGAGGCCAGCAGGATCCTGGGGCCAGGTAGTGTCCCCTGCTCTGACTGCCTG
GTCACTTTCATCCACCTTAAGTGTGACTCTTCTCGGAAGGGCAAGGGCAGGCGGGCCCGG
ACCCCCCCAGGCAAGGAGGTTACCCGGCTCACCCTGGAATTGGAAGCTGAGGTCAGAGCA
GAAGAAACGACAGCTGGCTGTGGGCTGCCTTGCCTCCGACAGCGGATGGAACGGAGGCTG
AAAGGGTCCCTGAAGATGCTCAGAAAGTCCATCAACCAAGACCGGTTCCTGCTGCGTCTG
GCAGGCCTTGACTATGAGCTAGCCCACAAGCCAGGCCTGGGAGCTGGGGACCGAGCAGAG
CTGGTGGAGGTCTGCCGACCTGGGCAGCACCGAGCAGGCACCAAGTGTGTCAGCTGCCCG
CAGGGAACGTATTACCACGGCCAGACGGAGCAGTGTGTGCCATGCCCAGCGGGCACCTTC
CAGGAGAGGGAAGGGCAGCTCTCCTGCGACCTTTGCCCTGGGAGTGATGCCCATGGGCCT
CTTGGAGCCACCAACGTCACCACGTGTGCAGGTCAGTGCCCACCTGGCCACCATTCAGGA
GATGGGTTTAAGCCCTGCCAGCCATGCCCCCGGGGCACCTATCAGCCTGAAGCAGGGCGA
ACCCTGTGCTTCCCCTGTGGCGGAGGCCTCACCACCAAGCATGAGGGGGCAGTCTCCTTC
CAGGACTGCGACACCAAAGTCCAGTGCTCCCCGGGCCACTACTATAACACTAGCATCCAT
CGCTGTATCCGCTGTGCTGTGGGCTCCTACCAGCCAGATTTCCGTCAGAACTTCTGCACC
CGCTGCCCAGGAAATACAAGCACTGACTTTGATGGCTCGACCAGTGTGGCCCAGTGCAAG
AATCGGCAGTGTGGCGGGGAGCTAGGTGAGTTCACTGGCTACATCGAGTCTCCCAACTAC
CCGGGCAACTACCCGGCCGGTGTGGAGTGCGTCTGGAACATCAACCCCCCACCCAAGCGC
AAGATCCTCATCGTGGTCCCCGAGATCTTCCTGCCCTCCGAGGATGAGTGTGGGGACGTC
CTGGTCATGAGAAAGAACTCCTCCCCATCCTCTATCACCACTTACGAGACCTGCCAGACC
TACGAGCGGCCCATTGCCTTCACCGCCCGGTCCAGGAAACTCTGGATCAACTTCAAGACC
AGTGAGGCCAACAGCGCTCGAGGCTTCCAGATTCCCTATGTGACCTACGATGAGGACTAT
GAGCAGCTGGTGGAAGACATAGTGCGTGATGGCCGGCTGTATGCCTCAGAAAACCACCAG
GAGATCCTAAAGGACAAGAAGCTTATCAAGGCCTTCTTTGAGGTCCTGGCCCACCCTCAA
AACTACTTCAAGTACACAGAGAAACACAAGGAGATGCTGCCAAAATCCTTCATCAAGCTA
CTGCGCTCCAAAGTCTCCAGCTTCCTGAGGCCTTACAAA
Protein sequence predicted based on nucleotide sequence
MGSGRVPGLCLLLLLVHARAAQHGKAAQDVDECVEGTDNCHIDAICQNTPRSYKCICKSG YTGDGKHCKDVDECEREDNAGCVHDCVNIPGNYRCTCYDGFHLAHDGHNCLDVDECAEGN GGCQQSCVNMMGSYECHCRDGFFLSDNQHTCIQRPEEGMNCMNKNHGCAHICRETPKGGI ACECRPGFELTKNQRDCKLTCNYGNGGCQHTCDDTEQGPRCGCHVKFVLHTDGKTCIETC AVNNGGCDSKCHDAATGVHCSCPVGFMLQPDRKTCKDIDECRLNNGGCDHICRNTVGSFE CSCKKGYKLLINERSCQDIDECSFDRTCDHMCVNTPGSFQCLCHRGYLLYGVTHCGDVDE CSINKGGCRFGCINTPGSYQCTCPAGQGRLHWNGKDCTEPVKCQSSLGASKAMLSCNRSG KKDTCALTCPSRARFLPESENGFTVSCGTPSPKAAPARVIHSGNSTVSSSCHEAAVLPVK QRASFKIKDAKCRLHLRNKGKAEEASRILGPGSVPCSDCLVTFIHLKCDSSRKGKGRRAR TPPGKEVTRLTLELEAEVRAEETTAGCGLPCLRQRMERRLKGSLKMLRKSINQDRFLLRL AGLDYELAHKPGLGAGDRAELVEVCRPGQHRAGTKCVSCPQGTYYHGQTEQCVPCPAGTF QEREGQLSCDLCPGSDAHGPLGATNVTTCAGQCPPGHHSGDGFKPCQPCPRGTYQPEAGR TLCFPCGGGLTTKHEGAVSFQDCDTKVQCSPGHYYNTSIHRCIRCAVGSYQPDFRQNFCT RCPGNTSTDFDGSTSVAQCKNRQCGGELGEFTGYIESPNYPGNYPAGVECVWNINPPPKR KILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKT SEANSARGFQIPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQ NYFKYTEKHKEMLPKSFIKLLRSKVSSFLRPYK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2982
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
