Gene:
Gene Symbol:
Uty
Gene Name:
ubiquitously transcribed tetratricopeptide repeat gene, Y chromosome
Original Clone ID:
FLH265368.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080746
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3636nts
Open reading frame: 1 to 3636
ATGAAATCTTACGGATTATCTCTCACTACTGCTGCCCTAGGTAATGAGGAAAAGAAAATG
GCGGCGGAAAAGGCTAGAGGCGAGGGCGAAGAGGGGTCCTTCAGTCTCACAGTCGAAGAG
AAGAAGGCGCTTTGTGGATTAGATAGCAGCTTCTTCGGGTTCTTGACCCGATGCAAAGAT
GGCGCCAAGATGAAGACGCTGTTGAACAAGGCAATTCACTTCTATGAGTCTTTAATAGTA
AAAGCTGAAGGAAAAGTGGAATCAGATTTCTTTTGTCAATTAGGTCACTTCAATCTCTTA
CTGGAAGATTATTCAAAAGCATTGTCATCTTACCAGAGATACTACAGTTTACAGACTGAC
TACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTATTTCTACTACAATGCA
TTTCAGTGGGCAATTAGAGCTTTTCAGGAAGTCCTTTATGTTGACCCTAATTTTTGCAGA
GCCAAAGAAATCCATTTACGACTTGGTTTTATGTTCAAAATGAATACAGATTATGAATCT
AGTTTAAAGCATTTTCAGCTGGCCCTGATCGACTGTAATGTCTGTACTTTGTCCAGTGTC
GAAATTCAGTTTCATATTGCTCACTTATATGAAACCCAGAGGAAATACCATTCTGCAAAG
GCAGCCTATGAACAACTTTTACAGATTGAAAGCTTGCCATCACAAGTCAAAGCAACTGTA
TTGCAACAGTTAGGTTGGATGCACCATAATATGGATCTAATTGGAGACAATACCACCAAA
GAGAGATATGCTATTCAGTATCTCCAAAAGTCATTGGAGGAAGATCCTAATTCTGGTCAA
TCTTGGTATTTCCTTGGAAGGTGTTACTCATGTATTGGGAAGGTTCAGGATGCCTTTGTA
TCTTACAGGCAATCCATTGATAAATCAGAAGCAAGTGCAGACACATGGTGTTCAATAGGT
GTGCTGTATCAACAGCAAAATCAACCTATGGATGCCTTGCAAGCCTATATATGTGCTGTA
CAGTTGGATCATGGACATGCAGCAGCCTGGATGGACTTAGGTATTCTTTATGAATCTTGC
AACCAACCCCAGGATGCCATTAAATGCTACTTAAATGCAGCTCGGAGCAAATCTTGTAAT
AACACCTCTGCACTTACATCAAGAATTAAGTTTTTACAGGCTCAGTTGTGTAACCTTCCA
CAAAGTAGTCTACAGAATAAAACTAAATTACTTCCTAGTATTGAGGAGGCATGGAGCCTA
CCAATACCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACAGCACAGCAGAGTGTT
TCTGATACCTGGAATAGTGTCCAGACAGCTTCACATCATTCAGTACAGCAGAAAGTTTAT
ACACAGTGCTTCACAGCACAGAAATTACAGAGTTTTGGTAAAGATCAACAACCTCCATTT
CAAACTGGGTCCACACGATATCTCCAAGCAGCTAGCACTAATGATCAGAATCAGAATGGA
AATCATACTCTGCCTCAGAATTCAAAAGGGGATGCTCAAAATCACTTCTTACGTATTCCT
ACCTCAGAAGAACAAAAGATCATTAACTTTACCAAAGAGAGCAAGGATTCAAGAAGTAAA
TCCTTGACTTCTAAAACAAGTAGGAAAGATAGAGACACATCTAATATCTGTGTTAATGCA
AAGAAGCATTCTAATCATATTTATCAGATCTCATCAGTTCCGATTTCCAGCCTTAATAAT
AAAGAATCTGTGTCACCAGATTTAATAATTGTAGACAATCCTCAGCTTTCTGTCTTAGTT
GGAGAAACTATCGACAATGTGGACCATGACATTGGAACTTGTGACAAAGTCAATAATGTT
CATCTAGCCATTCACAAAAAGCCTGATAATCTTTCTGCTTCTTCACCCTCTTCAGCCATT
TCCACAGAAACACTTTCTCTAAAGCTCACTGAACAGACACACATTGTTACCAGCTTTATC
AGTCCTCACAGTGGATTACACACAATTAATGGAGAAGGGCATGAGAACTTAGAGAGTTCT
GCCAGTGTTAATGTTGGCCTCAGACCTAGATCTCAAATCATACCATCAATGTCTGTGTCC
ATATACTCCAGTTCAACAGAAGTTCTGAAAGCGTGCAGGAGTCTAGGTAAAAATGGTTTG
TCTAATGGTCATATTCTCTTGGATATATGCCCACCTCCAAGACCACCAACTTCGCCATAT
CCTCCTTTACCAAAGGAGAAGTTGAATCCACCCACTCCTAGTATTTATTTGGAAAATAAG
CGAGATGCCTTTTTTCCTCCATTACATCAATTTTGCATCAATCCAAAAAACCCTGTTACA
GTAATTCGCGGTCTTGCTGGAGCTCTTAAGTTAGATCTTGGACTTTTCTCTACCAAAACT
TTGGTAGAGGCTAACAATGAACATATCGTAGAAGTGAGGACCCAATTGTTACAGCCAGCA
GATGAAAACTGGGATCCCTCTGGAACAAAGAAAATCTGGCGATATGAAAATAAAAGTTCT
CATACTACAATTGCTAAATATGCACAGTACCAGGCCTGCTCCTTCCAGGAATCATTGAGA
GAAGAAAATGAAAGAAGAACACAAGTTAAAGACTATTCAGATAATGAATCAACATGTTCA
GATAATTCTGGAAGGAGACAGAAAGCACCCTTTAAAACCATAAAATGTGGGATTAACATT
GATCTGTCTGATAACAAAAAGTGGAAGTTGCAGTTACATGAGCTGACAAAGCTTCCTGCT
TTTGTACGTGTTGTATCAGCAGGGAATCTTCTCAGTCATGTTGGCTATACTATATTGGGG
ATGAATTCAGTTCAACTATGTATGAAAGTTCCAGGGAGTAGAATACCAGGTCACCAAGAA
AATAACAATTTCTGTTCTGTTAACATAAATATTGGTCCAGGTGATTGTGAATGGTTTGTT
GTACCTGAAGATTATTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTC
CTGATGAGTTCTTGGTGGCCGAACCTTGAAGATCTTTATGAAGCAAATGTTCCTGTTTAT
AGGTTTATTCAACGACCTGGTGATTTGGTTTGGATAAATGCAGGCACTGTTCACTGGGTT
CAGGCTATTGGGTGGTGTAACAACATCACCTGGAATGTTGGTCCACTCACAGCCTTCCAA
TATAAATTGGCAGTTGAACGATATGAATGGAATAAATTGCAAAGTGTCAAGTCAGTAGTA
CCCATGGTTCATCTTTCATGGAATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTT
TTTGAAATGATTAAATATTGTCTCCTCAAGATTCTAAAGCATTGTCAGACTTTGAGAGAA
GCTCTTGTTGCAGCAGGGAAAGAGGTTTTGTGGCATGGGAGGATAAATGATGAACCAGCT
CCTTACTGTAGCATCTGTGAGGTGGAAGTTTTTAATTTACTTTTTGTCACTAATGAAAGC
AATTCTCAAAAGACATATATAGTACATTGTCAGAACTGTGCACGAAAAACAAGTGGAAAT
TTGGAAAATTTTGTAGTGCTAGAACAATACAAAATGGAGGACCTGATTCAAGTCTATGAC
CAATTTACATTAGCTCCTTCGTTATCTTCTGCATCT
Protein sequence predicted based on nucleotide sequence
MKSYGLSLTTAALGNEEKKMAAEKARGEGEEGSFSLTVEEKKALCGLDSSFFGFLTRCKD GAKMKTLLNKAIHFYESLIVKAEGKVESDFFCQLGHFNLLLEDYSKALSSYQRYYSLQTD YWKNAAFLYGLGLVYFYYNAFQWAIRAFQEVLYVDPNFCRAKEIHLRLGFMFKMNTDYES SLKHFQLALIDCNVCTLSSVEIQFHIAHLYETQRKYHSAKAAYEQLLQIESLPSQVKATV LQQLGWMHHNMDLIGDNTTKERYAIQYLQKSLEEDPNSGQSWYFLGRCYSCIGKVQDAFV SYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGILYESC NQPQDAIKCYLNAARSKSCNNTSALTSRIKFLQAQLCNLPQSSLQNKTKLLPSIEEAWSL PIPAELTSRQGAMNTAQQSVSDTWNSVQTASHHSVQQKVYTQCFTAQKLQSFGKDQQPPF QTGSTRYLQAASTNDQNQNGNHTLPQNSKGDAQNHFLRIPTSEEQKIINFTKESKDSRSK SLTSKTSRKDRDTSNICVNAKKHSNHIYQISSVPISSLNNKESVSPDLIIVDNPQLSVLV GETIDNVDHDIGTCDKVNNVHLAIHKKPDNLSASSPSSAISTETLSLKLTEQTHIVTSFI SPHSGLHTINGEGHENLESSASVNVGLRPRSQIIPSMSVSIYSSSTEVLKACRSLGKNGL SNGHILLDICPPPRPPTSPYPPLPKEKLNPPTPSIYLENKRDAFFPPLHQFCINPKNPVT VIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPSGTKKIWRYENKSS HTTIAKYAQYQACSFQESLREENERRTQVKDYSDNESTCSDNSGRRQKAPFKTIKCGINI DLSDNKKWKLQLHELTKLPAFVRVVSAGNLLSHVGYTILGMNSVQLCMKVPGSRIPGHQE NNNFCSVNINIGPGDCEWFVVPEDYWGVLNDFCEKNNLNFLMSSWWPNLEDLYEANVPVY RFIQRPGDLVWINAGTVHWVQAIGWCNNITWNVGPLTAFQYKLAVERYEWNKLQSVKSVV PMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKHCQTLREALVAAGKEVLWHGRINDEPA PYCSICEVEVFNLLFVTNESNSQKTYIVHCQNCARKTSGNLENFVVLEQYKMEDLIQVYD QFTLAPSLSSAS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
187
End on reference sequence
3825
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
