Gene:
Gene Symbol:
Bmpr2
Gene Name:
bone morphogenetic protein receptor, type II (serine/threonine kinase)
Original Clone ID:
FLH265558.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080935
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3114nts
Open reading frame: 1 to 3114
ATGACTTCCTCGCTGCATCGGCCCTTTCGGGTGCCCTGGCTGCTATGGGCCGTCCTGCTG
GTCAGCACTACGGCTGCTTCTCAGAATCAAGAACGGCTGTGTGCATTTAAAGATCCATAT
CAACAAGATCTTGGGATAGGTGAGAGTCGAATCTCTCATGAAAATGGGACAATATTATGT
TCCAAAGGGAGCACGTGTTATGGTCTGTGGGAGAAATCAAAAGGGGACATCAATCTTGTG
AAACAAGGATGTTGGTCTCACATCGGTGATCCCCAAGAGTGCCACTATGAAGAGTGTGTA
GTAACTACCACCCCACCCTCAATTCAGAATGGAACGTACCGCTTTTGCTGCTGTAGTACA
GATTTATGTAATGTCAACTTTACTGAGAACTTTCCACCCCCTGACACAACACCACTCAGT
CCACCTCATTCATTTAATCGAGATGAAACGATAATCATTGCTTTGGCATCAGTTTCTGTG
TTAGCTGTTTTGATAGTCGCCTTATGTTTTGGATACAGAATGTTGACAGGAGACCGGAAA
CAGGGTCTTCACAGCATGAACATGATGGAGGCGGCAGCAGCAGAGCCCTCCCTTGACCTG
GATAACCTGAAGCTGCTGGAGCTGATTGGACGGGGTCGATACGGAGCAGTATATAAAGGT
TCCTTGGATGAGCGTCCAGTTGCTGTAAAAGTATTTTCTTTTGCAAACCGTCAGAATTTT
ATAAATGAAAAAAACATTTACAGAGTGCCTTTGATGGAACATGACAACATTGCTCGCTTC
ATAGTTGGAGACGAGAGGCTCACTGCAGACGGCCGCATGGAGTATTTGCTTGTGATGGAG
TATTATCCCAATGGATCTCTGTGCAAATATCTGAGTCTCCACACAAGTGATTGGGTAAGC
TCTTGCCGTCTGGCTCATTCTGTGACTAGAGGACTGGCTTATCTTCACACAGAATTACCA
CGAGGAGATCATTATAAACCCGCAATCTCCCACCGAGATTTAAACAGCAGGAATGTCCTG
GTAAAGAATGACGGCGCGTGTGTTATCAGTGACTTTGGTTTATCCATGAGGCTAACTGGA
AATCGGCTGGTGCGCCCAGGGGAAGAAGATAATGCGGCTATAAGTGAGGTTGGCACAATT
CGCTATATGGCACCAGAAGTGCTAGAAGGAGCTGTGAACCTGAGGGACTGTGAGTCAGCT
CTGAAGCAAGTGGACATGTATGCGCTTGGACTCATCTACTGGGAGGTGTTTATGAGGTGT
ACAGACCTCTTCCCAGGTGAATCTGTACCAGATTACCAGATGGCTTTTCAGACAGAAGTT
GGAAACCATCCCACATTTGAGGATATGCAGGTTCTTGTGTCCAGAGAGAAGCAGAGACCC
AAGTTCCCAGAAGCCTGGAAAGAAAATAGCCTGGCAGTGAGGTCACTCAAGGAAACAATT
GAAGACTGCTGGGACCAGGATGCAGAGGCTCGGCTCACTGCACAGTGTGCTGAGGAGAGG
ATGGCTGAACTCATGATGATATGGGAGAGAAACAAGTCTGTGAGCCCAACGGTCAACCCA
ATGTCAACTGCTATGCAGAATGAACGCAACCTGTCACATAATAGGCGTGTGCCAAAAATC
GGGCCTTACCCAGATTATTCCTCTTCCTCATATATTGAAGACTCTATCCATCATACTGAC
AGCATTGTGAAGAATATTTCCTCTGAGCATTCGATGTCCAGCACACCATTGACAATAGGA
GAAAAGAATCGAAATTCAATTAATTATGAACGACAGCAAGCACAAGCTCGAATCCCTAGC
CCAGAAACAAGCGTCACAAGCCTGTCCACAAACACAACCACCACAAACACCACCGGCCTC
ACTCCAAGTACTGGCATGACCACTATATCTGAGATGCCATACCCAGATGAGACACATTTG
CACGCCACAAATGTTGCACAGTCAATCGGGCCAACCCCTGTCTGCTTACAGCTGACAGAA
GAAGACTTGGAGACTAATAAGCTAGATCCAAAAGAAGTTGATAAGAACCTCAAGGAAAGC
TCTGATGAGAATCTCATGGAGCATTCTCTGAAGCAGTTCAGTGGGCCAGACCCATTGAGC
AGTACCAGTTCTAGCTTGCTTTATCCACTCATAAAGCTCGCAGTGGAAGTGACTGGACAA
CAGGACTTCACACAGGCTGCAAATGGGCAAGCATGTTTAATTCCTGATGTTCCACCTGCT
CAGATCTATCCTCTCCCTAAGCAACAGAACCTTCCTAAGAGACCTACTAGTTTGCCTTTG
AACACCAAAAATTCAACAAAAGAACCCCGGCTAAAATTTGGCAACAAGCACAAATCAAAC
TTGAAACAAGTAGAAACTGGAGTTGCCAAGATGAATACAATCAATGCAGCAGAGCCTCAT
GTGGTGACAGTAACTATGAATGGTGTGGCAGGTAGAAGCCACAATGTTAATTCTCATGCT
GCCACAACCCAGTATGCCAATGGCGCAGTGCCAGCTGGCCAGGCAGCCAACATAGTGGCA
CATAGGTCCCAAGAAATGCTGCAGAATCAATTTATTGGTGAGGATACCAGGCTGAATATC
AATTCCAGTCCTGATGAGCATGAACCTTTACTGAGACGAGAGCAACAGGCTGGCCATGAT
GAAGGGGTTCTGGATCGTTTGGTAGATAGGAGGGAACGGCCATTAGAAGGTGGCCGAACA
AATTCCAATAACAACAACAGCAATCCATGTTCAGAACAAGATATCCTTACACAAGGTGTT
ACAAGCACAGCTGCAGATCCTGGGCCATCAAAGCCCAGAAGAGCACAGAGGCCCAATTCT
CTGGATCTTTCAGCCACAAATATCCTGGATGGCAGCAGTATACAGATAGGTGAGTCAACA
CAAGATGGCAAATCAGGATCAGGTGAAAAGATCAAGAGACGTGTGAAAACTCCATACTCT
CTTAAGCGGTGGCGCCCGTCCACCTGGGTCATCTCCACCGAGCCGCTGGACTGTGAGGTC
AACAACAATGGCAGTGACAGGGCAGTCCATTCTAAATCTAGCACTGCTGTGTACCTTGCA
GAGGGAGGCACTGCCACGACCACAGTGTCTAAAGATATAGGAATGAATTGTCTG
Protein sequence predicted based on nucleotide sequence
MTSSLHRPFRVPWLLWAVLLVSTTAASQNQERLCAFKDPYQQDLGIGESRISHENGTILC SKGSTCYGLWEKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCST DLCNVNFTENFPPPDTTPLSPPHSFNRDETIIIALASVSVLAVLIVALCFGYRMLTGDRK QGLHSMNMMEAAAAEPSLDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNF INEKNIYRVPLMEHDNIARFIVGDERLTADGRMEYLLVMEYYPNGSLCKYLSLHTSDWVS SCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGACVISDFGLSMRLTG NRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEVFMRC TDLFPGESVPDYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETI EDCWDQDAEARLTAQCAEERMAELMMIWERNKSVSPTVNPMSTAMQNERNLSHNRRVPKI GPYPDYSSSSYIEDSIHHTDSIVKNISSEHSMSSTPLTIGEKNRNSINYERQQAQARIPS PETSVTSLSTNTTTTNTTGLTPSTGMTTISEMPYPDETHLHATNVAQSIGPTPVCLQLTE EDLETNKLDPKEVDKNLKESSDENLMEHSLKQFSGPDPLSSTSSSLLYPLIKLAVEVTGQ QDFTQAANGQACLIPDVPPAQIYPLPKQQNLPKRPTSLPLNTKNSTKEPRLKFGNKHKSN LKQVETGVAKMNTINAAEPHVVTVTMNGVAGRSHNVNSHAATTQYANGAVPAGQAANIVA HRSQEMLQNQFIGEDTRLNINSSPDEHEPLLRREQQAGHDEGVLDRLVDRRERPLEGGRT NSNNNNSNPCSEQDILTQGVTSTAADPGPSKPRRAQRPNSLDLSATNILDGSSIQIGEST QDGKSGSGEKIKRRVKTPYSLKRWRPSTWVISTEPLDCEVNNNGSDRAVHSKSSTAVYLA EGGTATTTVSKDIGMNCL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
17
End on reference sequence
3133
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
