DNASU Plasmid | Egr4 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Egr4

Gene Name: early growth response 4

Original Clone ID:

FLH265741.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00081117

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1434nts

Open reading frame: 1 to 1434

ATGCTCCACCTGAGCGACTTCTCCGGCTCCGACGCGCTTCTCTCCAAGCCCACCGAAGGC
TGCGCCCACGCCAGCCCCGAGCTGCCCCGGCTGCCTGCTAGGGACGCTCCCTCGGCCGCC
GCGTATCCTGGAGGCGACTTCTTGAGCTGGGCTTTGAGCAGCTGCGGCGCCGGGGGGGAC
TTAACAGACTCCTGCTTCCTGGAGGGCCCTGCACCCACGCCCCCTTCGGGCCTCAGCTAC
AGCGGCAGCTTCTTCATCCAGGCGGTTCCCGAACACCCGCACGACCCGGAGGCCCTCTTC
AACCTCATGTCTGGCATCTTGGGCCTGGCACCCTTCCCCGGTCCCGAGGCGGCAGCGTCT
CGGTCCCCCCTGGATGTCCCTTTCCCCGCGGGCCCCGATGCCTTGCTGCCGGACCTCTAC
TCCCCAGATCTGAGTTCGGCCGCCTTTCCGGAGGCGTTTTGGGAGGCAGCGCCTTCGGCG
GGCGCTCCCTCGCAGTGCCTGTTCGAGCCCCAGCTCTCCCCGCCCGACGTCAAGCCAGGG
CTGCGGGCGCCTCCCGCTTCGCCAGCGCTGGACGCTGCTGCTTCAGCCTTCAAAGGGCCC
TATGCCCCCTGGGAGCTGCTGTCGGCCGGGGTCCCGGGGAACTGTGGGTCGCAGGGGAGC
TTCCAGACCACCCCGGAGGCCCGCTTCTCTGCAGTAGGGACCAAGGTCGAGGACCTGCTG
TCCATCAGCTGCCCCGCCGAGCTGCCCGGCCCGGCCGCCAGACTCTACCAGGCAGGGGCC
TACGACACCTTCTCGTTGGCCCCAGGTGACTTAGGGGAGGGGACCGAGGGCCTCCCGGCG
CTGCTGACCCCTCCCGGCGGGGAGGGAGGGAGCGGCGGCGAAGGCGGAGAGTTCCTGGCC
GCCCCTCCGGCGCAGCTGTCCCCTCTAGGCCTGCGCGGTGCCGCCACGGCGGACTTCTCC
AAACCCCTGGTGGCCGATCTCCCGGGGGGCAGCGGCGTGGCCGCGCCCTCATCCCCCGCC
GCATCCTTCCCCGCGGCCAAAGCCCGACGCAAGGGACGCCGGGGCGGCAAGTGCAGCGCG
CGCTGCTTCTGCCCGCGGCCGCACGTCAAGGCCTTCGCCTGCCCGGTGGAGAGCTGCGTG
CGGAGCTTCGCGCGCTCCGACGAGCTCAACCGCCACCTGCGCATCCACACGGGCCACAAG
CCCTTCCAGTGCCGCATCTGCCTGCGCAACTTCAGCCGCAGCGACCACCTCACCACGCAC
GTGCGCACCCACACGGGCGAGAAGCCCTTCGCCTGCGACGTGTGTGGCCGCCGCTTCGCG
CGCAGTGACGAGAAGAAACGGCACAGCAAGGTGCACCTAAAGCAGAAGGCGCGCGCGGAG
GAGCGCCTCAAGGGCCTGGGCTTCTACTCGCTGGGCCTCTCCTTCGCCGCGCTG

Protein sequence predicted based on nucleotide sequence

MLHLSDFSGSDALLSKPTEGCAHASPELPRLPARDAPSAAAYPGGDFLSWALSSCGAGGD
LTDSCFLEGPAPTPPSGLSYSGSFFIQAVPEHPHDPEALFNLMSGILGLAPFPGPEAAAS
RSPLDVPFPAGPDALLPDLYSPDLSSAAFPEAFWEAAPSAGAPSQCLFEPQLSPPDVKPG
LRAPPASPALDAAASAFKGPYAPWELLSAGVPGNCGSQGSFQTTPEARFSAVGTKVEDLL
SISCPAELPGPAARLYQAGAYDTFSLAPGDLGEGTEGLPALLTPPGGEGGSGGEGGEFLA
APPAQLSPLGLRGAATADFSKPLVADLPGGSGVAAPSSPAASFPAAKARRKGRRGGKCSA
RCFCPRPHVKAFACPVESCVRSFARSDELNRHLRIHTGHKPFQCRICLRNFSRSDHLTTH
VRTHTGEKPFACDVCGRRFARSDEKKRHSKVHLKQKARAEERLKGLGFYSLGLSFAAL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 146

End on reference sequence 1582

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Egr4

Symbol Nomenclature :

Egr4

SYNONYM :

NGF1-C; NGFI-C; NGFIC; pAT133

Designation :

EGR-4|early growth response protein 4

Full Nomenclature :

early growth response 4

GENEID :

13656

GI :

146327648

GenBank Accession :

BC141509

Mouse Genome Informatics :

99252

Target GenBank:

NM_020596

Protein

SMART domain :

ZnF_C2H2 : zinc finger

UniProt :

Q3URA9

UniProt :

Q9WUF2

Clone

Cloning Information :

265741

HIP Master Clone ID :

197839

IMAGE ID :

100014496

Original Clone ID :

FLH265741.01F

Organism

Species Specific ID:

13656

Genome

Chromosome :

Map Location :

6 C3|6 37.48 cM

Ensembl :

ENSMUSG00000071341

Reagents

Labome :

Egr4-antibody