Gene:
Gene Symbol:
Cntn3
Gene Name:
contactin 3
Original Clone ID:
FLH265815.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00081191
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3084nts
Open reading frame: 1 to 3084
ATGATGTTGTCATGGAAACAGTTGATCCTGCTTTCATTCATTGGCTGCCTGGCAGGTGAA
CTTCTTTTACAAGGCCCTGTGTTTATCAAAGAGCCCAGCAACAGTATTTTCCCTGTTGAT
TCAGAGGACAAAAAAATAACATTAAATTGTGAAGCAAGAGGCAACCCTTCACCTCATTAC
AGGTGGCAGCTGAATGGCAGTGATATTGATACAAGTCTGGACCATCGTTACAAACTGAAT
GGAGGAAATCTCATAGTTATTAACCCTAACAGAAATTGGGATACAGGAAGTTACCAGTGT
TTTGCAACGAATTCTCTTGGAACAATAGTGAGCAGAGAAGCCAAACTCCAGTTTGCCTAT
CTTGAAAATTTTAAGACCAGGATGAGAAGCACTGTGTCGGTCCGTGAAGGCCAGGGAGTG
GTGCTTCTCTGTGGACCCCCACCTCACTCAGGAGAACTGTCATATGCTTGGGTCTTCAAT
GAGTATCCATCATTTGTTGAAGAAGACAGTCGACGATTTGTCTCTCAAGAGACAGGACAC
CTCTACATAGCTAAGGTGGAACCATCTGATGTGGGAAATTACACATGTGTGGTGACCAGC
ACGGTGACAAATACCAGAGTATTGGGCTCCCCAACCCCTTTGGTGCTACGCTCTGATGGT
GTCATGGGTGAATATGAACCCAAAATAGAAGTCCAGTTTCCAGAGACTCTTCCAGCAGCT
AAAGGTTCAACTGTGAGGTTGGAATGCTTCGCCCTGGGAAACCCTGTTCCTCAGATTAAT
TGGAGAAGAAGTGATGGGATGCCGTTTCCCAACAAAATTAAGTTGAGGAAGTTCAATGGT
ATGCTGGAAATTCAAAACTTCCAACAGGAAGATACAGGCTCCTATGAGTGTATTGCTGAA
AATTCACGAGGAAAAAATGTGGCGAGAGGGCGGCTCACTTACTATGCAAAACCTTATTGG
CTTCAACTCTTAAGAGATGTGGAAATTGCTGTGGAGGACAGTCTTTATTGGGAATGCCGG
GCAAGTGGGAAGCCTAAACCCTCTTATCGATGGCTGAAAAATGGAGATGCCTTAGTACTA
GAGGAGAGAATACAGATAGAAAATGGTGCCCTTACAATAACCAACCTCAATGTGACTGAC
TCTGGAATGTTCCAATGTATAGCAGAAAACAAGCATGGTCTCATCTACTCCAGTGCTGAG
CTCAAGGTAGTAGCTTCTGCTCCAGATTTTTCAAGAAACCCTATGAAAAAAATGGTCCAG
GTGCAGGTGGGCAGCCTGGTGATCCTGGATTGTAAACCCAGAGCCTCTCCAAGGGCACTT
TCTTTCTGGAAGAAGGGAGACATGATGGTGCGGGAGCAGGCAAGGGTTTCTTTTTTAAAT
GATGGAGGACTCAAAATCATGAATGTGACTAAGGCAGACGCTGGAACCTACACCTGCACA
GCAGAAAACCAGTTTGGGAAAGCAAATGGCACAACCCACTTGGTTGTGACAGAACCTACG
AGAATAATTTTGGCACCTTCAAACATGGACGTTGCTGTTGGTGAGAGCGTCATTTTACCC
TGCCAGGTTCAACATGATCCCCTATTAGACATAATGTTTGCCTGGTATTTCAATGGAGCC
CTTACAGACTTTAAGAAAGATGGATCTCACTTTGAGAAAGTTGGTGGGAGTTCATCTGGT
GATTTAATGATCAGAAACATTCAATTGAAACACAGTGGGAAGTACGTATGTATGGTGCAG
ACAGGGGTGGACAGTGTTTCCTCTGCAGCTGAGCTCATCGTGAGAGGTTCACCTGGGCCA
CCGGAAAATGTGAAGGTAGATGAAATTACAGACACAACAGCCCAGCTCTCTTGGACGGAA
GGCACGGACAGTCATAGCCCAGTTATATCGTATGCAGTCCAAGCTAGGACGCCTTTCTCT
GTGGGCTGGCAAAGTGTCAGAACAGTACCTGAGGTCATTGATGGCAAGACACATACAGCT
ACTGTCGTGGAGTTAAACCCATGGGTGGAATATGAATTTCGCATTGTAGCCAGTAACAAA
ATCGGAGGTGGAGAACCCAGTTTACCCTCAGAAAAAGTAAGGACTGAAGAGGCAGCTCCA
GAAATCGCACCTTCTGAGGTCAGCGGAGGAGGTGGAAGCCGATCTGAACTAGTGATAACC
TGGGATCCAGTCCCTGAAGAACTACAGAATGGAGGAGGTTTTGGGTATGTGGTTGCTTTC
CGCCCACTTGGGGTTACCACCTGGATCCAGACAGTGGTGACATCCCCAGATAACCCAAGA
TACGTCTTTAGGAATGAAAGCATTGTGCCCTTTTCGCCATATGAAGTTAAAGTGGGAGTT
TACAATAACAAAGGTGAAGGACCCTTTAGCCCAGTGACAACTGTGTTCTCTGCAGAGGAA
GAGCCTACAGTTGCCCCATCTCACATCTCTGCCCATAGCCTGTCTTCCTCAGAAATTGAA
GTTTCTTGGAATACAATTCCCTGGAAGTTGAGCAATGGACATTTACTGGGCTATGAGGTG
CGGTACTGGAACAATGGAGGAGAAGAAGAATCATCCCGCAAAGTGAAAGTGGCAGGAAAT
CAGACATCAGCTGTGCTCCGAGGCCTGAAGAGCAACCTGGCCTATTACACAGCTGTCCGG
GCTTACAACAGCGCAGGCGCTGGTCCCTTCAGCGCCACAGTTAATGCGACCACCAAGAAA
ACCCCTCCCAGTCAGCCACCAGGAAATGTTGTTTGGAATGCTACAGACACTAAAGTGCTA
CTTAATTGGGAACAAGTGAAAGCCATGGAGAATGAATCAGAAGTAACAGGCTACAAGGTT
TTCTATAGGACTAGCAGTCAGAATAATGTGCACGTGCTGAACACAAATAAAACTTCAGCT
GAACTGTTGCTGCCTATCAAAGAAGACTACATTATTGAGGTCAAGGCCACCACAGATGGA
GGGGATGGCACCAGTAGTGAGCAGATCAGGATTCCAAGAATAACCAGTATGGATGCAAGA
GGATCCACGTCAGCCATTTCTAACATCCACCCTTTGTCAGGTTATATGTCTGTCCTACTG
TTCTTTATTGTAAATGCTTTGTGG
Protein sequence predicted based on nucleotide sequence
MMLSWKQLILLSFIGCLAGELLLQGPVFIKEPSNSIFPVDSEDKKITLNCEARGNPSPHY RWQLNGSDIDTSLDHRYKLNGGNLIVINPNRNWDTGSYQCFATNSLGTIVSREAKLQFAY LENFKTRMRSTVSVREGQGVVLLCGPPPHSGELSYAWVFNEYPSFVEEDSRRFVSQETGH LYIAKVEPSDVGNYTCVVTSTVTNTRVLGSPTPLVLRSDGVMGEYEPKIEVQFPETLPAA KGSTVRLECFALGNPVPQINWRRSDGMPFPNKIKLRKFNGMLEIQNFQQEDTGSYECIAE NSRGKNVARGRLTYYAKPYWLQLLRDVEIAVEDSLYWECRASGKPKPSYRWLKNGDALVL EERIQIENGALTITNLNVTDSGMFQCIAENKHGLIYSSAELKVVASAPDFSRNPMKKMVQ VQVGSLVILDCKPRASPRALSFWKKGDMMVREQARVSFLNDGGLKIMNVTKADAGTYTCT AENQFGKANGTTHLVVTEPTRIILAPSNMDVAVGESVILPCQVQHDPLLDIMFAWYFNGA LTDFKKDGSHFEKVGGSSSGDLMIRNIQLKHSGKYVCMVQTGVDSVSSAAELIVRGSPGP PENVKVDEITDTTAQLSWTEGTDSHSPVISYAVQARTPFSVGWQSVRTVPEVIDGKTHTA TVVELNPWVEYEFRIVASNKIGGGEPSLPSEKVRTEEAAPEIAPSEVSGGGGSRSELVIT WDPVPEELQNGGGFGYVVAFRPLGVTTWIQTVVTSPDNPRYVFRNESIVPFSPYEVKVGV YNNKGEGPFSPVTTVFSAEEEPTVAPSHISAHSLSSSEIEVSWNTIPWKLSNGHLLGYEV RYWNNGGEEESSRKVKVAGNQTSAVLRGLKSNLAYYTAVRAYNSAGAGPFSATVNATTKK TPPSQPPGNVVWNATDTKVLLNWEQVKAMENESEVTGYKVFYRTSSQNNVHVLNTNKTSA ELLLPIKEDYIIEVKATTDGGDGTSSEQIRIPRITSMDARGSTSAISNIHPLSGYMSVLL FFIVNALW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3171
Start on reference sequence
85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
