Gene:
Gene Symbol:
PXDN
Gene Name:
peroxidasin homolog (Drosophila)
Original Clone ID:
FLH265853.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081229
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCAAGCGCTCCAGGGGCCCCGGGCGCCGCTGCCTGTTGGCGCTCGTGCTGTTCTGC
GCCTGGGGGACGCTGGCCGTGGTGGCCCAGAAGCCGGGCGCAGGGTGTCCGAGCCGCTGC
CTGTGCTTCCGCACCACCGTGCGCTGCATGCATCTGCTGCTGGAGGCCGTGCCCGCCGTG
GCGCCGCAGACCTCCATCCTAGATCTTCGCTTTAACAGAATCAGAGAGATCCAACCTGGG
GCATTCAGGCGGCTGAGGAACTTGAACACATTGCTTCTCAATAATAATCAGATCAAGAGG
ATACCTAGTGGAGCATTTGAAGACTTGGAAAATTTAAAATATCTCTATCTGTACAAGAAT
GAGATCCAGTCAATTGACAGGCAAGCATTTAAGGGACTTGCCTCTCTAGAGCAACTATAC
CTGCACTTTAATCAGATAGAAACTTTGGACCCAGATTCGTTCCAGCATCTCCCGAAGCTC
GAGAGGCTATTTTTGCATAACAACCGGATTACACATTTAGTTCCAGGGACATTTAATCAC
TTGGAATCTATGAAGAGATTGCGACTGGACTCAAACACACTTCACTGCGACTGTGAAATC
CTGTGGTTGGCGGATTTGCTGAAAACCTACGCGGAGTCGGGGAACGCGCAGGCAGCGGCC
ATCTGTGAATATCCCAGACGCATCCAGGGACGCTCAGTGGCAACCATCACCCCGGAAGAG
CTGAACTGTGAAAGGCCCCGGATCACCTCCGAGCCCCAGGACGCAGATGTGACCTCGGGG
AACACCGTGTACTTCACCTGCAGAGCCGAAGGCAACCCCAAGCCTGAGATCATCTGGCTG
CGAAACAATAATGAGCTGAGCATGAAGACAGATTCCCGCCTAAACTTGCTGGACGATGGG
ACCCTGATGATCCAGAACACACAGGAGACAGACCAGGGTATCTACCAGTGCATGGCAAAG
AACGTGGCCGGAGAGGTGAAGACGCAAGAGGTGACCCTCAGGTACTTCGGGTCTCCAGCT
CGACCCACTTTTGTAATCCAGCCACAGAATACAGAGGTGCTGGTTGGGGAGAGCGTCACG
CTGGAGTGCAGCGCCACAGGCCACCCCCCGCCGCGGATCTCCTGGACGAGAGGTGACCGC
ACACCCTTGCCAGTTGACCCGCGGGTGAACATCACGCCTTCTGGCGGGCTTTACATACAG
AACGTCGTACAGGGGGACAGCGGAGAGTATGCGTGCTCTGCGACCAACAACATTGACAGC
GTCCATGCCACCGCTTTCATCATCGTCCAGGCTCTTCCTCAGTTCACTGTGACGCCTCAG
GACAGAGTCGTTATTGAGGGCCAGACCGTGGATTTCCAGTGTGAAGCCAAGGGCAACCCG
CCGCCCGTCATCGCCTGGACCAAGGGAGGGAGCCAGCTCTCCGTGGACCGGCGGCACCTG
GTCCTGTCATCGGGAACACTTAGAATCTCTGGTGTTGCCCTCCACGACCAGGGCCAGTAC
GAATGCCAGGCTGTCAACATCATCGGCTCCCAGAAGGTCGTGGCCCACCTGACTGTGCAG
CCCAGAGTCACCCCAGTGTTTGCCAGCATTCCCAGCGACACAACAGTGGAGGTGGGCGCC
AATGTGCAGCTCCCGTGCAGCTCCCAGGGCGAGCCCGAGCCAGCCATCACCTGGAACAAG
GATGGGGTTCAGGTGACAGAAAGTGGAAAATTTCACATCAGCCCTGAAGGATTCTTGACC
ATCAATGACGTTGGCCCTGCAGACGCAGGTCGCTATGAGTGTGTGGCCCGGAACACCATT
GGGTCGGCCTCGGTGAGCATGGTGCTCAGTGTGAATGTTCCTGACGTCAGTCGAAATGGA
GATCCGTTTGTAGCTACCTCCATCGTGGAAGCGATTGCGACTGTTGACAGAGCTATAAAC
TCAACCCGAACACATTTGTTTGACAGCCGTCCTCGTTCTCCAAATGATTTGCTGGCCTTG
TTCCGGTATCCGAGGGATCCTTACACAGTTGAACAGGCACGGGCGGGAGAAATCTTTGAA
CGGACATTGCAGCTCATTCAGGAGCATGTACAGCATGGCTTGATGGTCGACCTCAACGGA
ACAAGTTACCACTACAACGACCTGGTGTCTCCACAGTACCTGAACCTCATCGCAAACCTG
TCGGGCTGTACCGCCCACCGGCGCGTGAACAACTGCTCGGACATGTGCTTCCACCAGAAG
TACCGGACGCACGACGGCACCTGTAACAACCTGCAGCACCCCATGTGGGGCGCCTCGCTG
ACCGCCTTCGAGCGCCTGCTGAAATCCGTGTACGAGAATGGCTTCAACACCCCTCGGGGC
ATCAACCCCCACCGACTGTACAACGGGCACGCCCTTCCCATGCCGCGCCTGGTGTCCACC
ACCCTGATCGGGACGGAGACCGTCACACCCGACGAGCAGTTCACCCACATGCTGATGCAG
TGGGGCCAGTTCCTGGACCACGACCTCGACTCCACGGTGGTGGCCCTGAGCCAGGCACGC
TTCTCCGACGGACAGCACTGCAGCAACGTGTGCAGCAACGACCCCCCCTGCTTCTCTGTC
ATGATCCCCCCCAATGACTCCCGGGCCAGGAGCGGGGCCCGCTGCATGTTCTTCGTGCGC
TCCAGCCCTGTGTGCGGCAGCGGCATGACTTCGCTGCTCATGAACTCCGTGTACCCGCGG
GAGCAGATCAACCAGCTCACCTCCTACATAGACGCATCCAACGTGTACGGGAGCACGGAG
CATGAGGCCCGCAGCATCCGCGACCTGGCCAGCCACCGCGGCCTGCTGCGGCAGGGCATC
GTGCAGCGGTCCGGGAAGCCGCTGCTCCCCTTCGCCACCGGGCCGCCCACGGAGTGCATG
CGGGACGAGAACGAGAGCCCCATCCCCTGCTTCCTGGCCGGGGACCACCGCGCCAACGAG
CAGCTGGGCCTGACCAGCATGCACACGCTGTGGTTCCGCGAGCACAACCGCATTGCCACG
GAGCTGCTCAAGCTGAACCCGCACTGGGACGGCGACACCATCTACTATGAGACCAGGAAG
ATCGTGGGTGCGGAGATCCAGCACATCACCTACCAGCACTGGCTCCCGAAGATCCTGGGG
GAGGTGGGCATGAGGACGCTGGGAGAGTACCACGGCTACGACCCCGGCATCAATGCTGGC
ATCTTCAACGCCTTCGCCACCGCGGCCTTCAGGTTTGGCCACACGCTTGTCAACCCACTG
CTTTACCGGCTGGACGAGAACTTCCAGCCCATTGCACAAGATCACCTCCCCCTTCACAAA
GCTTTCTTCTCTCCCTTCCGGATTGTGAATGAGGGCGGCATCGATCCGCTTCTCAGGGGG
CTGTTCGGGGTGGCGGGGAAAATGCGTGTGCCCTCGCAGCTGCTGAACACGGAGCTCACG
GAGCGGCTGTTCTCCATGGCACACACGGTGGCTCTGGACCTGGCGGCCATCAACATCCAG
CGGGGCCGGGACCACGGGATCCCACCCTACCACGACTACAGGGTCTACTGCAATCTATCG
GCGGCACACACGTTCGAGGACCTGAAAAATGAGATTAAAAACCCTGAGATCCGGGAGAAA
CTGAAAAGGTTGTATGGCTCGACACTCAACATCGACCTGTTTCCGGCGCTCGTGGTGGAG
GACCTGGTGCCTGGCAGCCGGCTGGGCCCCACCCTGATGTGTCTTCTCAGCACACAGTTC
AAGCGCCTGCGAGATGGGGACAGGTTGTGGTATGAGAACCCTGGGGTGTTCTCCCCGGCC
CAGCTGACTCAGATCAAGCAGACGTCGCTGGCCAGGATCCTATGCGACAACGCGGACAAC
ATCACCCGGGTGCAGAGCGACGTGTTCAGGGTGGCGGAGTTCCCTCACGGCTACGGCAGC
TGTGACGAGATCCCCAGGGTAGACCTCCGGGTGTGGCAGGACTGCTGTGAAGACTGTAGG
ACCAGGGGGCAGTTCAATGCCTTTTCCTATCATTTCCGAGGCAGACGGTCTCTTGAGTTC
AGCTACCAGGAGGACAAGCCGACCAAGAAAACAAGACCACGGAAAATACCCAGTGTTGGG
AGACAGGGGGAACATCTCAGCAACAGCACCTCAGCCTTCAGCACACGCTCAGATGCATCT
GGGACAAATGACTTCAGAGAGTTTGTTCTGGAAATGCAGAAGACCATCACAGACCTCAGA
ACACAGATAAAGAAACTTGAATCACGGCTCAGTACCACAGAGTGCGTGGATGCCGGGGGC
GAATCTCACGCCAACAACACCAAGTGGAAAAAAGATGCATGCACCATTTGTGAATGCAAA
GACGGGCAGGTCACCTGCTTCGTGGAAGCTTGCCCCCCTGCCACCTGTGCTGTCCCCGTG
AACATCCCAGGGGCCTGCTGTCCAGTCTGCTTACAGAAGAGGGCGGAGGAAAAGCCC
Protein sequence predicted based on nucleotide sequence
MAKRSRGPGRRCLLALVLFCAWGTLAVVAQKPGAGCPSRCLCFRTTVRCMHLLLEAVPAV APQTSILDLRFNRIREIQPGAFRRLRNLNTLLLNNNQIKRIPSGAFEDLENLKYLYLYKN EIQSIDRQAFKGLASLEQLYLHFNQIETLDPDSFQHLPKLERLFLHNNRITHLVPGTFNH LESMKRLRLDSNTLHCDCEILWLADLLKTYAESGNAQAAAICEYPRRIQGRSVATITPEE LNCERPRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIWLRNNNELSMKTDSRLNLLDDG TLMIQNTQETDQGIYQCMAKNVAGEVKTQEVTLRYFGSPARPTFVIQPQNTEVLVGESVT LECSATGHPPPRISWTRGDRTPLPVDPRVNITPSGGLYIQNVVQGDSGEYACSATNNIDS VHATAFIIVQALPQFTVTPQDRVVIEGQTVDFQCEAKGNPPPVIAWTKGGSQLSVDRRHL VLSSGTLRISGVALHDQGQYECQAVNIIGSQKVVAHLTVQPRVTPVFASIPSDTTVEVGA NVQLPCSSQGEPEPAITWNKDGVQVTESGKFHISPEGFLTINDVGPADAGRYECVARNTI GSASVSMVLSVNVPDVSRNGDPFVATSIVEAIATVDRAINSTRTHLFDSRPRSPNDLLAL FRYPRDPYTVEQARAGEIFERTLQLIQEHVQHGLMVDLNGTSYHYNDLVSPQYLNLIANL SGCTAHRRVNNCSDMCFHQKYRTHDGTCNNLQHPMWGASLTAFERLLKSVYENGFNTPRG INPHRLYNGHALPMPRLVSTTLIGTETVTPDEQFTHMLMQWGQFLDHDLDSTVVALSQAR FSDGQHCSNVCSNDPPCFSVMIPPNDSRARSGARCMFFVRSSPVCGSGMTSLLMNSVYPR EQINQLTSYIDASNVYGSTEHEARSIRDLASHRGLLRQGIVQRSGKPLLPFATGPPTECM RDENESPIPCFLAGDHRANEQLGLTSMHTLWFREHNRIATELLKLNPHWDGDTIYYETRK IVGAEIQHITYQHWLPKILGEVGMRTLGEYHGYDPGINAGIFNAFATAAFRFGHTLVNPL LYRLDENFQPIAQDHLPLHKAFFSPFRIVNEGGIDPLLRGLFGVAGKMRVPSQLLNTELT ERLFSMAHTVALDLAAINIQRGRDHGIPPYHDYRVYCNLSAAHTFEDLKNEIKNPEIREK LKRLYGSTLNIDLFPALVVEDLVPGSRLGPTLMCLLSTQFKRLRDGDRLWYENPGVFSPA QLTQIKQTSLARILCDNADNITRVQSDVFRVAEFPHGYGSCDEIPRVDLRVWQDCCEDCR TRGQFNAFSYHFRGRRSLEFSYQEDKPTKKTRPRKIPSVGRQGEHLSNSTSAFSTRSDAS GTNDFREFVLEMQKTITDLRTQIKKLESRLSTTECVDAGGESHANNTKWKKDACTICECK DGQVTCFVEACPPATCAVPVNIPGACCPVCLQKRAEEKP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4504
Start on reference sequence
65
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
