Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Gene synthesis by Gen9
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00813516
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3585nts
Open reading frame: 1 to 3585
ATGACAGTCATGTCCCTTTCCAGGGACCTCAAGGACGACTTTCACAGTGACACGGTACTC
TCCATCTTAAATGAGCAGCGCATTCGGGGCATTTTATGCGATGTCACTATCATTGTGGAA
GATACCAAATTTAAAGCCCATAGCAATGTTCTGGCAGCTTCAAGCCTGTATTTTAAAAAT
ATCTTTTGGAGCCATACAATCTGTATTTCCAGCCACGTCCTGGAGCTGGACGATCTCAAA
GCTGAAGTGTTTACTGAAATACTTAATTATATCTACAGTTCCACAGTCGTTGTCAAGAGA
CAGGAAACAGTCACTGATCTCGCAGCTGCAGGAAAAAAGCTGGGAATATCGTTCTTGGAA
GACCTTACTGATCGCAACTTCTCAAATTCCCCGGGTCCCTATGTATTCTGTATTACTGAA
AAGGGAGTGGTTAAAGAAGAAAAAAATGAAAAAAGGCATGAAGAACCAGCCATCACTAAT
GGGCCAAGGATCACAAATGCATTTTCCATCATCGAAACAGAAAATAGTAATAACATGTTT
TCCCCGCTGGACTTGAGGGCAAGTTTCAAAAAAGTCTCCGACTCCATGAGAACAGCTAGC
CTTTGCCTGGAGAGGACGGACGTCTGCCACGAGGCAGAGCCTGTCCGCACACTTGCCGAG
CACTCATACGCTGTTTCTTCCGTAGCTGAAGCTTACAGAAGTCAGCCTGTACGTGAACAT
GATGGCAGTTCACCTGGTAACACAGGGAAAGAAAATTGTGAAGCCCTTGCAGCGAAACCG
AAAACATGCCGGAAGCCAAAGACATTCTCCATACCACAGGATTCGGATTCAGCCACAGAA
AATATACCACCCCCTCCAGTATCCAACTTAGAGGTTAATCAAGAAAGAAGTCCACAACCA
GCTGCTGTTCTCACTCGTTCAAAATCTCCAAACAATGAAGGAGATGTCCATTTTTCCAGG
GAAGATGAAAATCAATCTTCTGATGTTCCCGGGCCGCCAGCCGCAGAGGTTCCACCTCTG
GTGTACAATTGTAGCTGCTGTTCCAAAGCCTTTGACAGCAGCACTCTGCTCAGTGCCCAC
ATGCAGCTTCACAAGCCAACCCAGGAGCCTTTAGTGTGCAAGTATTGCAACAAACAATTC
ACCACCCTGAACAGGTTGGATCGGCATGAACAGATCTGCATGAGGTCAAGCCACATGCCC
ATTCCTGGAGGAAACCAACGCTTTTTAGAAAACTATCCTACCATTGGACAAAATGGAGGT
TCATTCACAGGTCCAGAACCTTTATTATCTGAAAATAGGATTGGTGAATTTTCCAGTACC
GGAAGTACTTTGCCAGACACGGACCACATGGTTAAATTTGTTAATGGGCAAATGCTCTAC
AGTTGCGTTGTGTGCAAACGTAGTTATGTGACCTTATCTAGCCTCCGAAGACATGCAAAT
GTTCACTCCTGGAGAAGAACATATCCTTGCCATTACTGCAACAAAGTATTTGCATTGGCT
GAGTACAGGACAAGGCATGAAATTTGGCATACGGGAGAAAGACGATATCAGTGCATTTTC
TGTCTTGAAACTTTCATGACCTACTATATACTCAAAAATCATCAGAAGTCTTTCCATGCC
ATCGATCATAGACTTTCCATCAGTAAAAAAACAGCAAATGGAGGCTTGAAGCCTAGTGTC
TATCCGTATAAACTTTATAGGCTACTGCCTATGAAATGCAAGAGAGCCCCTTATAAGAGC
TACCGAAATTCTTCCTATGAAAATGCACGAGAAAACAGTCAAATGAATGAGTCTGCACCT
GGTACCTATGTTGTTCAGAATCCACACAGCTCTGAATTACCAACGCTGAATTTCCAAGAT
ACTGTAAACACCCTGACCAACAGTCCAGCCATCCCATTGGAAACATCTGCATGTCAGGAC
ATACCCACTTCTGCCAATGTACAAAATGCAGAGGGTACCAAATGGGGAGAGGAGGCATTG
AAAATGGATCTTGACAATAACTTTTATTCAACTGAGGTGTCAGTTTCTTCCACTGAAAAT
GCTGTCAGTTCTGACCTCCGGGCAGGGGATGTACCTGTTTTATCTTTGAGTAATAGCAGT
GAGAATGCCGCCTCTGTGATCAGCTACAGTGGCTCTGCACCCTCGGTCATTGTACACAGC
AGCCAGTTTTCATCGGTGATCATGCACAGCAATGCCATTGCTGCCATGACCAGCAGCAAC
CACAGAGCCTTTTCAGACCCAGCTGTCAGTCAGTCCCTGAAAGATGACAGTAAGCCCGAG
CCAGATAAAGTGGGTAGGTTTGCAAGCAGACCCAAAAGCATTAAGGAGAAAAAGAAAACT
ACATCACATACCAGGGGAGAAATACCGGAGGAGTCAAACTATGTTGCTGATCCTGGAGGA
TCACTGAGCAAAACCACAAATATTGCTGAAGAAACCAGCAAAATTGAAACCTACATTGCA
AAACCTGCTCTGCCGGGAACCTCCACAAATAGTAATGTTGCACCCCTTTGCCAAATAACA
GTGAAAATTGGAAACGAAGCCATTGTGAAAAGGCACATTCTAGGATCTAAATTGTTTTAT
AAAAGAGGGAGAAGACCCAAGTATCAGATGCAGGAGGAGCCTTTGCCACAGGGGAATGAC
CCAGAACCCAGTGGAGACAGCCCACTCGGGCTTTGCCAATCCGAGTGCATGGAGATGAGT
GAAGTGTTCGATGACGCAAGTGACCAGGATTCCACTGACAAACCGTGGCGCCCTTACTAC
AACTACAAACCCAAAAAGAAATCCAGACAGTTGAAAAAAATGAGGAAAGTCAACTGGAGG
AAGGAGCACGGAAACAGGAGCCCGAGCCATAAATGTAAATACCCAGCAGAACTGGATTGC
GCCGTGGGGAAGGCTCCTCAGGATAAACCCTTTGAGGAAGAAGAAACTAAAGAGATGCCC
AAGCTGCAGTGTGAACTCTGTGATGGAGACAAAGCAGTGGGGGCTGGAAACCAAGGAAGG
CCCCACCGACATCTTACTTCTCGGCCATATGCCTGCGAGCTCTGCGCCAAGCAGTTCCAG
AGCCCTTCCACACTCAAAATGCACATGAGATGTCACACCGGGGAGAAGCCATACCAGTGC
AAGACCTGCGGACGGTGCTTTTCGGTGCAAGGAAACTTACAGAAACATGAACGCATCCAC
CTGGGCTTGAAGGAGTTCGTCTGTCAGTATTGCAACAAGGCATTCACCTTGAATGAAACC
CTCAAAATCCATGAAAGAATCCATACTGGAGAAAAGCGTTACCACTGTCAGTTCTGCTTT
CAGAGATTTTTGTATCTCTCCACCAAAAGGAATCACGAGCAGAGGCATATTCGGGAGCAT
AATGGGAAGGGCTATGCCTGCTTCCAGTGCCCCAAAATTTGCAAAACAGCTGCTGCCCTT
GGAATGCACCAAAAGAAACACTTATTCAAAAGCCCAAGTCAGCAGGAGAAAATAGGTGAC
GTGTGCCACGAAAACTCAAATCCCTTGGAGAATCAACATTTCATTGGTTCAGAAGACAAT
GACCAAAAGGATAACATACAAACCGGTGTGGAAAATGTTGTCCTT
Protein sequence predicted based on nucleotide sequence
MTVMSLSRDLKDDFHSDTVLSILNEQRIRGILCDVTIIVEDTKFKAHSNVLAASSLYFKN IFWSHTICISSHVLELDDLKAEVFTEILNYIYSSTVVVKRQETVTDLAAAGKKLGISFLE DLTDRNFSNSPGPYVFCITEKGVVKEEKNEKRHEEPAITNGPRITNAFSIIETENSNNMF SPLDLRASFKKVSDSMRTASLCLERTDVCHEAEPVRTLAEHSYAVSSVAEAYRSQPVREH DGSSPGNTGKENCEALAAKPKTCRKPKTFSIPQDSDSATENIPPPPVSNLEVNQERSPQP AAVLTRSKSPNNEGDVHFSREDENQSSDVPGPPAAEVPPLVYNCSCCSKAFDSSTLLSAH MQLHKPTQEPLVCKYCNKQFTTLNRLDRHEQICMRSSHMPIPGGNQRFLENYPTIGQNGG SFTGPEPLLSENRIGEFSSTGSTLPDTDHMVKFVNGQMLYSCVVCKRSYVTLSSLRRHAN VHSWRRTYPCHYCNKVFALAEYRTRHEIWHTGERRYQCIFCLETFMTYYILKNHQKSFHA IDHRLSISKKTANGGLKPSVYPYKLYRLLPMKCKRAPYKSYRNSSYENARENSQMNESAP GTYVVQNPHSSELPTLNFQDTVNTLTNSPAIPLETSACQDIPTSANVQNAEGTKWGEEAL KMDLDNNFYSTEVSVSSTENAVSSDLRAGDVPVLSLSNSSENAASVISYSGSAPSVIVHS SQFSSVIMHSNAIAAMTSSNHRAFSDPAVSQSLKDDSKPEPDKVGRFASRPKSIKEKKKT TSHTRGEIPEESNYVADPGGSLSKTTNIAEETSKIETYIAKPALPGTSTNSNVAPLCQIT VKIGNEAIVKRHILGSKLFYKRGRRPKYQMQEEPLPQGNDPEPSGDSPLGLCQSECMEMS EVFDDASDQDSTDKPWRPYYNYKPKKKSRQLKKMRKVNWRKEHGNRSPSHKCKYPAELDC AVGKAPQDKPFEEEETKEMPKLQCELCDGDKAVGAGNQGRPHRHLTSRPYACELCAKQFQ SPSTLKMHMRCHTGEKPYQCKTCGRCFSVQGNLQKHERIHLGLKEFVCQYCNKAFTLNET LKIHERIHTGEKRYHCQFCFQRFLYLSTKRNHEQRHIREHNGKGYACFQCPKICKTAAAL GMHQKKHLFKSPSQQEKIGDVCHENSNPLENQHFIGSEDNDQKDNIQTGVENVVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
