Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Source: Gene synthesis by Gen9; codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Gene synthesis by Gen9
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00813518
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3876nts
Open reading frame: 1 to 3876
ATGGACGCATCCTATGATGGTACTGAGGTAACTGTCGTGATGGAGGAAATTGAGGAAGCC
TATTGTTACACCTCTCCTGGGCCACCCAAGAAGAAGAAAAAGTATAAAATCCATGGAGAA
AAGACCAAGAAACCCAGGTCTGCTTACCTTTTGTACTATTACGACATCTACCTGAAAGTG
CAGCAGGAGCTCCCCCACCTCCCTCAGTCTGAGATCAATAAGAAGATTAGTGAGAGTTGG
AGGCTTCTCAGCGTGGCCGAGAGAAGTTACTACTTGGAGAAAGCCAAACTAGAGAAGGAA
GGTTTGGATCCTAACTCTAAGCTCTCTGCACTGACTGCTGTGGTTCCGGACATCCCAGGT
TTCCGCAAGATCCTCCCACGCTCAGATTATATCATCATCCCCAAGAGCAGCCTGCAGGAG
GACCGGAGCTGCCCTCAGCTGGAGCTATGTGTGGCTCAGAACCAGATGTCCCCGAAAGGA
CCTCCTCTTGTGTCTAACACTGCCCCAGAGACAGTGCCTAGCCATGCAGGAATGGCAGAG
CAGTGCCTTGCTGTAGAGGCCCTGGCAGAGGAGGTTGGAGCCTTAACCCAGTCAGGTGCT
GTACAGGAGATTGCCACCTCAGAGATCCTCAGCCAGGATGTGCTCCTGGAGGACGCTTCC
CTAGAAGTAGGGGAGAGCCACCAACCTTACCAGACAAGCCTGGTAATTGAAGAAACCTTG
GTGAATGGCTCACCAGACCTCCCCACTGGAAGCCTGGCTGTGCCCCACCCCCAGGTTGGC
GAGAGTGTATCAGTGGTAACAGTCATGAGGGATTCCAGTGAGAGCAGTTCCTCTGCGCCC
GCCACACAGTTCATCATGTTGCCTCTGCCTGCCTACTCGGTTGTGGAGAACCCCACTTCC
ATCAAACTGACCACTACATATACCCGCCGGGGCCATGGGACATGCACCAGCCCAGGGTGC
TCCTTTACATATGTCACCAGGCACAAGCCACCTAAGTGCCCTACCTGTGGTAACTTCCTA
GGAGGGAAGTGGATCCCAAAGGAAAAGCCAGCCAAAGTAAAAGTGGAATTGGCTTCTGGC
GTCTCTTCCAAAGGCTCCGTGGTGAAAAGAAATCAGCAACCTGTCACCACTGAGCAAAAT
TCCTCTAAGGAAAATGCCTCCAAACTGACTCTGGAGAACTCGGAAGCTGTAAGCCAGCTC
CTGAACGTAGCTCCTCCCAGAGAAGTAGGTGAGGAGAGTGAGTGGGAGGAAGTGATCATC
TCCGATGCCCATGTTTTGGTTAAGGAAGCTCCCGGGAATTGTGGTACAGCCGTCACTAAG
ACGCCAGTCGTCAAAAGTGGTGTGCAGCCTGAGGTCACTCTGGGGACAACTGACAATGAT
AGCCCTGGAGCAGACGTACCAACACCATCCGAGGGGACAAGTACCTCCAGTCCACTCCCT
GCTCCTAAAAAACCTACAGGAGTTGACCTGCTTACCCCTGGGTCTAGAGCTCCAGAGCTT
AAAGGCAGAGCACGGGGCAAGCCCTCATTACTCGCTGCAGCAAGACCCATGAGAGCAATT
TTGCCAGCCCCAGTTAACGTGGGGAGAGGCAGCAGCATGGGACTCCCCAGGGCCAGGCAG
GCCTTTTCTCTGAGTGATAAGACTCCCTCTGTGAGAACTTGTGGTCTGAAGCCAAGCACA
CTGAAGCAGCTGGGCCAGCCCATTCAACAGCCATCCGGCCCTGGTGAGGTGAAGCTACCA
AGTGGCCCATCCAACAGGACTTCTCAGGTGAAAGTTGTGGAGGTCAAGCCCGATATGTTT
CCTCCATATAAGTACAGCTGCACTGTCACACTGGATTTGGGCCTCGCCACATCAAGAGGC
CGGGGAAAGTGCAAGAATCCCTCTTGTAGCTATGTCTACACCAACAGACACAAACCCCGA
ATTTGTCCCAGCTGTGGTGTTAACCTTGCCAAAGACCGGACTGAGAAAACCACCAAGGCT
ATCGAGGTGAGTTCACCACTCCCAGATGTACTGAATGCCACAGAGCCCCTGAGTACAGCC
CAGAGGGAGATCCAGCGCCAGTCCACACTGCAGCTGCTGCGCAAAGTCCTGCAGATTCCT
GAGAATGAGTCAGAGCTCGCTGAGGTCTTCGCCTTGATTCATGAACTCAACAGCTCTCGA
CTTATCTTGTCCAACGTGAGTGAGGAGACAGTCACCATCGAGCAAACCTCTTGGTCGAAT
TATTATGAGTCTCCGTCCACGCAGTGCCTTCTCTGTAGCAGTCCATTATTCAAAGGGGGA
CAAAACTCCCTGGCTGGGCCCCAGGAATGCTGGCTGCTGACAGCCAGTCGTTTGCAGACA
GTGACTGCCCAGGTGAAGATGTGTCTGAACCCCCATTGTCTGGCCCTGCACAGCTTCATA
GACATCTACACAGGCCTCTTTAATGTGGGGAACAAGCTGCTGGTAAGTCTGGACTTGCTG
TTTGCAATCAGAAATCAAATCAAGCTCGGAGAGGACCCCAGAGTGTCCATCAATGTTGTT
CTGAAGTCGGTGCAGGAGCAGACAGAGAAGACTCTGACCTCGGAGGAGCTGAGCCAGCTG
CAAGAGCTGCTGTGCAATGGCTATTGGGCCTTTGAGTGCCTCACCGTCCGAGACTACAAT
GACATGATCTGTGGCATCTGTGGTGTGGCCCCCAAAGTGGAAATGGCTCAGAGGAGTGAA
GAGAATGTGCTAGCACTGAAGAGCGTGGAGTTCACCTGGCCTGAGTTCCTGGGCTCTAAT
GAGGTAAATGTGGAGGACTTTTGGGCCACGATGGAGACAGAGGTGATTGAGCAGGTCGCA
TTTCCTGCCAGCATCCCTATCACCAAATTTGATGCGTCTGTTATTGCCCCCTTCTTCCCA
CCACTCATGAGAGGAGCTGTGGTCGTCAACACTGAGAAAGACAAAAACCTGGATGTGCAG
CCAGTACCTGGCAGCGGCAGTGCATTGGTGAGGCTGCTACAGGAGGGCACCTGTAAGCTG
GACGAGATTGGCTCCTACAGCGAAGAGAAGCTTCAGCACCTTCTAAGGCAGTGCGGAATC
CCCTTTGGGGCCGAAGACTCCAAGGACCAGCTCTGCTTCTCATTGTTGGCCCTCTACGAA
TCTGTACAGAATGGAGCTAGAGCTATACGGCCCCCACGTCACTTCACAGGAGGTAAAATC
TACAAGGTGTGCCCTCATCAGGTGGTCTGCGGCTCCAAGTATCTTGTGCGAGGTGAGAGT
GCCCGTGACCATGTGGACCTGCTTGCTTCTTCCCGCCACTGGCCTCCTGTCTATGTGGTA
GATATGGCCACGTCAGTGGCCCTGTGTGCTGACCTCTGCTACCCTGAGCTGACTAACCAG
ATGTGGGGGCGGAACCAGGGCTGTTTCTCTAGCCCTACAGAGCCACCTGTGAGCGTGTCC
TGCCCAGAGCTCTTGGACCAGCATTATACTGTGGACATGACAGAAACTGAGCACTCCATC
CAGCACCCAGTCACCAAGACTGCAACGCGGCGCATCGTCCATGCAGGCCTACAGCCCAAT
CCTGGTGATCCTAGTGCTGGGCACCACTCTTTAGCCCTGTGCCCTGAATTGGCACCTTAC
GCAACCATCCTGGCCTCCATTGTGGACAGCAAACCAAACGGTGTCCGCCAGCGGCCCATT
GCCTTCGACAATGCCACTCACTATTACCTCTACAACCGGCTCATGGACTTTCTCACCAGC
CGCGAAATTGTCAATCGCCAGATCCATGACATTGTACAGAGCTGCCAGCCTGGTGAAGTG
GTCATTCGTGACACCCTCTACCGCCTTGGGGTTGCTCAGATCAAGACAGAGACGGAGGAG
GAGGGTGAGGAAGAGGAGGTGGCCGCAGTGGCAGAA
Protein sequence predicted based on nucleotide sequence
MDASYDGTEVTVVMEEIEEAYCYTSPGPPKKKKKYKIHGEKTKKPRSAYLLYYYDIYLKV QQELPHLPQSEINKKISESWRLLSVAERSYYLEKAKLEKEGLDPNSKLSALTAVVPDIPG FRKILPRSDYIIIPKSSLQEDRSCPQLELCVAQNQMSPKGPPLVSNTAPETVPSHAGMAE QCLAVEALAEEVGALTQSGAVQEIATSEILSQDVLLEDASLEVGESHQPYQTSLVIEETL VNGSPDLPTGSLAVPHPQVGESVSVVTVMRDSSESSSSAPATQFIMLPLPAYSVVENPTS IKLTTTYTRRGHGTCTSPGCSFTYVTRHKPPKCPTCGNFLGGKWIPKEKPAKVKVELASG VSSKGSVVKRNQQPVTTEQNSSKENASKLTLENSEAVSQLLNVAPPREVGEESEWEEVII SDAHVLVKEAPGNCGTAVTKTPVVKSGVQPEVTLGTTDNDSPGADVPTPSEGTSTSSPLP APKKPTGVDLLTPGSRAPELKGRARGKPSLLAAARPMRAILPAPVNVGRGSSMGLPRARQ AFSLSDKTPSVRTCGLKPSTLKQLGQPIQQPSGPGEVKLPSGPSNRTSQVKVVEVKPDMF PPYKYSCTVTLDLGLATSRGRGKCKNPSCSYVYTNRHKPRICPSCGVNLAKDRTEKTTKA IEVSSPLPDVLNATEPLSTAQREIQRQSTLQLLRKVLQIPENESELAEVFALIHELNSSR LILSNVSEETVTIEQTSWSNYYESPSTQCLLCSSPLFKGGQNSLAGPQECWLLTASRLQT VTAQVKMCLNPHCLALHSFIDIYTGLFNVGNKLLVSLDLLFAIRNQIKLGEDPRVSINVV LKSVQEQTEKTLTSEELSQLQELLCNGYWAFECLTVRDYNDMICGICGVAPKVEMAQRSE ENVLALKSVEFTWPEFLGSNEVNVEDFWATMETEVIEQVAFPASIPITKFDASVIAPFFP PLMRGAVVVNTEKDKNLDVQPVPGSGSALVRLLQEGTCKLDEIGSYSEEKLQHLLRQCGI PFGAEDSKDQLCFSLLALYESVQNGARAIRPPRHFTGGKIYKVCPHQVVCGSKYLVRGES ARDHVDLLASSRHWPPVYVVDMATSVALCADLCYPELTNQMWGRNQGCFSSPTEPPVSVS CPELLDQHYTVDMTETEHSIQHPVTKTATRRIVHAGLQPNPGDPSAGHHSLALCPELAPY ATILASIVDSKPNGVRQRPIAFDNATHYYLYNRLMDFLTSREIVNRQIHDIVQSCQPGEV VIRDTLYRLGVAQIKTETEEEGEEEEVAAVAE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
