Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
U0687BI160-56
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00814545
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6180nts
Open reading frame: 1 to 6180
ATGGCTTCCCCTCTGAGGGACGAGGAGGAGGAGGAGGAGGAGATGGTGGTGTCGGAGGAG
GAAGAAGAGGAGGAAGAAGAGGGCGACGAGGAGGAGGAGGAGGAGGTGGAGGCGGCCGAC
GAGGACGATGAGGAGGACGACGACGAGGGAGTACTCGGGCGCGGGCCGGGCCACGACCGG
GGCCGCGACCGCCACAGCCCCCCCGGCTGCCACCTCTTCCCGCCGCCGCCGCCGCCGCCG
CCACCGCTGCCCCCGCCGCCGCCGCCCCCGCCGCCAGATAAGGATGACATTCGGCTGCTG
CCGTCAGCATTGGGTGTGAAGAAGAGAAAACGAGGACCCAAGAAGCAGAAGGAGAACAAG
CCAGGAAAACCCCGAAAACGCAAGAAGCGTGACAGTGAGGAGGAATTTGGTTCTGAGCGA
GATGAGTACCGGGAGAAGTCAGAGAGTGGGGGCAGTGAATATGGAACCGGACCGGGTCGG
AAACGAAGAAGGAAGCACCGAGAAAAAAAGGAGAAGAAGACAAAGCGGCGGAAAAAGGGG
GAGGGAGATGGGGGGCAAAAGCAAGTGGAACAGAAGTCATCAGCAACTCTGCTTCTGACC
TGGGGCCTGGAGGATGTGGAGCATGTGTTCTCTGAGGAGGATTACCACACGCTCACCAAC
TACAAAGCCTTCAGCCAGTTCATGAGGCCCCTAATTGCTAAGAAGAATCCTAAGATCCCA
ATGTCTAAGATGATGACCATCCTTGGGGCCAAATGGAGAGAGTTCAGTGCCAACAACCCC
TTCAAGGGGTCAGCAGCTGCTGTGGCGGCGGCAGCGGCAGCAGCAGCAGCAGCTGTAGCT
GAGCAGGTGTCAGCTGCTGTCTCGTCGGCCACCCCCATAGCACCCTCCGGACCCCCCGCC
CTTCCACCACCCCCTGCTGCTGATATCCAGCCCCCACCCATCCGAAGAGCCAAAACCAAA
GAGGGCAAAGGTCCAGGCCATAAGAGGCGGAGTAAGAGCCCCCGAGTGCCTGATGGACGC
AAGAAGCTTCGGGGAAAGAAAATGGCACCACTCAAAATAAAACTAGGGCTTCTGGGTGGC
AAGAGGAAGAAAGGAGGCTCGTATGTTTTTCAGAGCGACGAAGGTCCTGAACCAGAGGCT
GAGGAATCAGACCTGGACAGTGGCAGTGTCCACAGTGCCTCAGGCCGGCCTGATGGCCCT
GTCCGCACCAAGAAACTAAAGAGAGGCCGGCCAGGAAGGAAGAAGAAGAAGGTCCTGGGC
TGTCCTGCAGTGGCCGGGGAGGAGGAGGTTGATGGCTACGAGACGGATCACCAGGATTAC
TGTGAGGTGTGCCAGCAGGGTGGGGAAATTATTCTGTGTGACACCTGCCCTCGTGCCTAC
CACCTCGTCTGCCTTGATCCTGAGCTTGACCGGGCTCCAGAGGGCAAATGGAGCTGCCCT
CACTGTGAGAAGGAGGGGGTCCAGTGGGAGGCCAAGGAGGAAGAAGAAGAATACGAAGAG
GAGGGAGAGGAAGAAGGGGAGAAGGAGGAGGAGGATGATCACATGGAGTACTGCCGCGTA
TGCAAGGACGGCGGGGAGCTCCTGTGCTGTGACGCGTGCATCTCCTCCTACCACATTCAT
TGTCTAAACCCTCCCCTGCCTGACATTCCCAATGGTGAATGGCTGTGTCCCCGATGCACA
TGCCCCGTGCTGAAGGGTCGAGTGCAGAAGATCCTACATTGGCGGTGGGGGGAGCCACCT
GTAGCAGTGCCAGCCCCTCAACAGGCAGATGGAAATCCAGATGTCCCACCCCCCCGTCCT
CTTCAAGGCAGATCAGAGCGAGAGTTCTTTGTCAAGTGGGTAGGACTATCCTACTGGCAC
TGCTCCTGGGCCAAGGAGCTTCAGCTGGAAATCTTCCATTTGGTTATGTATCGAAACTAC
CAGCGGAAGAATGACATGGATGAGCCCCCACCCCTGGACTATGGCTCCGGCGAGGATGAT
GGGAAGAGCGACAAGCGTAAAGTGAAAGACCCGCACTATGCTGAGATGGAGGAGAAGTAC
TATCGTTTTGGCATCAAGCCAGAGTGGATGACCGTCCACCGCATCATCAACCACAGTGTG
GATAAAAAGGGGAATTACCACTATCTAGTAAAATGGAGGGACTTACCATATGACCAGTCC
ACGTGGGAGGAAGATGAAATGAATATCCCTGAATACGAAGAACATAAGCAAAGCTACTGG
AGACACCGAGAACTAATTATGGGGGAAGACCCTGCCCAGCCCCGCAAGTATAAGAAGAAG
AAGAAGGAGCTACAGGGTGATGGGCCTCCCAGTTCTCCCACTAATGATCCTACCGTGAAA
TATGAGACTCAGCCACGGTTTATCACAGCCACTGGAGGCACCCTGCACATGTATCAGTTG
GAAGGGCTGAACTGGCTACGCTTCTCCTGGGCCCAGGGCACTGACACCATTCTAGCTGAT
GAGATGGGGCTAGGCAAGACCATACAAACCATCGTCTTCCTCTACTCACTCTACAAGGAG
GGCCACACAAAAGGTCCCTTCCTGGTGAGTGCCCCACTCTCTACCATCATTAACTGGGAG
CGGGAGTTCCAGATGTGGGCACCCAAATTCTATGTGGTGACATACACGGGTGACAAGGAC
AGCCGGGCCATCATTCGTGAGAATGAATTCTCCTTTGAGGACAATGCCATCAAAGGGGGC
AAGAAAGCTTTTAAGATGAAGAGGGAGGCACAGGTGAAGTTCCATGTTCTCCTGACATCG
TATGAGCTGATCACCATTGATCAGGCAGCACTTGGTTCCATCCGCTGGGCCTGTCTTGTG
GTAGATGAGGCCCATCGACTCAAGAACAACCAGTCCAAGTTTTTCAGGGTTCTCAATGGT
TACAAGATAGATCATAAGTTGCTGCTGACAGGAACCCCATTGCAGAATAATCTGGAGGAG
CTCTTCCATCTCCTGAACTTCCTCACCCCAGAGAGATTTAACAACTTGGAGGGCTTCCTG
GAGGAGTTTGCTGACATATCCAAAGAGGACCAGATCAAGAAACTGCATGATTTGCTGGGG
CCACACATGCTGCGGAGACTCAAGGCAGATGTCTTTAAGAACATGCCAGCCAAGACAGAG
CTCATCGTTCGGGTGGAGCTAAGCCCCATGCAGAAGAAATACTACAAATACATCCTGACT
CGAAATTTTGAGGCCTTGAATTCACGAGGTGGTGGGAACCAGGTGTCGCTGCTTAATATC
ATGATGGATCTTAAGAAGTGCTGCAACCATCCATACCTTTTTCCCGTGGCTGCTATGGAG
TCCCCCAAACTCCCCAGTGGGGCTTATGAGGGTGGGGCACTTATTAAGTCGTCTGGGAAG
CTCATGCTGCTCCAGAAGATGCTGCGAAAGCTGAAGGAGCAAGGACACCGAGTGCTCATC
TTCTCGCAGATGACCAAAATGTTAGACTTGCTTGAGGACTTCTTAGACTATGAAGGCTAC
AAGTATGAGCGCATCGATGGTGGTATCACGGGTGCCCTGAGGCAGGAGGCCATCGATCGG
TTTAATGCTCCTGGGGCCCAACAATTCTGCTTCCTCCTGTCCACCCGAGCTGGGGGCCTG
GGCATCAATCTGGCCACTGCTGACACTGTCATCATCTTTGATTCTGACTGGAACCCCCAT
AATGACATCCAGGCCTTTAGCCGGGCTCATCGGATTGGCCAGGCCAACAAAGTGATGATT
TACCGGTTTGTGACTCGCGCGTCAGTGGAAGAGCGAATCACACAAGTGGCCAAGAGAAAG
ATGATGCTGACACACCTGGTTGTGCGGCCTGGGCTGGGCTCCAAGGCAGGCTCCATGTCC
AAGCAGGAGCTTGACGACATTCTCAAATTTGGCACTGAAGAGCTATTCAAGGATGAAAAC
GAGGGGGAGAACAAGGAGGAGGACAGCAGTGTGATTCATTATGACAATGAGGCCATCGCT
CGGCTGTTGGACCGGAACCAGGATGCAACTGAGGACACTGACGTGCAGAACATGAATGAG
TATCTCAGCTCCTTCAAGGTGGCACAGTACGTCGTGCGGGAAGAAGACAAGATTGAGGAA
ATTGAGCGAGAGATCATCAAGCAGGAGGAGAATGTGGACCCTGACTACTGGGAGAAGCTG
CTGAGGCATCACTATGAGCAACAGCAGGAAGACCTAGCCCGGAATCTAGGCAAGGGCAAG
CGGGTTCGCAAGCAAGTTAACTACAATGATGCTGCTCAGGAAGACCAAGACAACCAGTCA
GAGTACTCGGTGGGTTCAGAGGAGGAGGATGAAGACTTCGATGAACGTCCTGAAGGGCGT
AGACAGTCAAAGAGGCAGCTCCGGAATGAGAAAGATAAGCCACTGCCTCCACTGCTGGCC
CGAGTCGGGGGCAACATTGAGGTGCTGGGCTTCAACACCCGTCAGCGGAAGGCTTTCCTC
AATGCTGTGATGCGCTGGGGGATGCCACCACAGGATGCCTTCACCACACAGTGGCTGGTG
CGGGACCTGAGGGGCAAGACTGAGAAGGAGTTTAAGGCCTATGTGTCTTTGTTCATGCGC
CATCTGTGTGAGCCTGGGGCAGACGGCTCTGAAACCTTTGCCGATGGGGTCCCTCGGGAG
GGACTGAGTCGCCAGCAGGTGTTGACCCGCATTGGAGTCATGTCTCTCGTCAAAAAGAAG
GTGCAGGAGTTTGAGCACATCAATGGGCGTTGGTCAATGCCGGAACTGATGCCTGACCCC
AGCGCCGATTCTAAGCGCTCCTCCAGAGCCTCCTCTCCTACCAAAACGTCTCCCACCACT
CCTGAGGCTTCTGCTACCAACAGTCCCTGCACCTCTAAACCTGCTACTCCAGCTCCAAGT
GAGAAAGGAGAAGGCATAAGGACACCTCTTGAGAAGGAGGAAGCTGAAAACCAGGAGGAA
AAGCCAGAGAAGAACAGCAGAATTGGGGAGAAGATGGAGACAGAGGCTGATGCCCCCAGC
CCAGCCCCATCACTTGGGGAGCGGCTGGAGCCAAGGAAGATTCCTCTAGAGGATGAGGTG
CCAGGGGTGCCTGGAGAGATGGAGCCTGAACCTGGGTACCGTGGGGACAGAGAGAAGTCA
GCCACAGAGTCGACGCCAGGAGAAAGGGGGGAGGAGAAGCCGTTGGATGGACAGGAACAC
AGGGAGAGGCCGGAGGGGGAAACAGGGGATTTGGGCAAGAGAGAAGATGTAAAAGGTGAC
CGGGAGCTTCGACCAGGGCCTCGAGATGAGCCACGGTCCAATGGGCGACGAGAGGAAAAG
ACAGAGAAGCCCCGGTTCATGTTCAATATCGCCGATGGTGGCTTCACAGAGCTTCACACA
CTGTGGCAGAATGAGGAACGGGCAGCTATTTCCTCGGGGAAACTCAATGAGATCTGGCAC
AGAAGACATGACTATTGGCTTCTGGCTGGGATTGTCCTCCATGGCTATGCACGGTGGCAG
GACATCCAGAATGATGCTCAATTTGCCATTATCAACGAGCCATTTAAAACTGAAGCCAAT
AAGGGGAACTTTCTGGAGATGAAAAATAAGTTCCTGGCCCGGAGGTTCAAGCTCCTGGAG
CAGGCGCTGGTGATTGAGGAGCAGCTGCGGCGGGCGGCCTACCTGAACCTGTCGCAGGAG
CCGGCGCACCCCGCCATGGCCCTCCACGCCCGCTTCGCCGAGGCCGAGTGCCTGGCCGAG
AGCCACCAGCACCTCTCCAAGGAGTCGCTGGCGGGGAACAAGCCGGCCAACGCCGTCCTG
CACAAGGTTCTGAACCAGCTGGAGGAGTTGCTGAGCGACATGAAGGCGGACGTGACCCGC
CTGCCAGCCACGCTGTCCCGAATACCCCCCATCGCAGCCCGCCTTCAGATGTCCGAGCGC
AGCATCCTCAGCCGGCTGGCCAGCAAGGGCACGGAGCCTCACCCCACACCGGCCTACCCG
CCGGGTCCCTACGCTACACCTCCGGGGTACGGGGCGGCCTTCAGCGCCGCACCCGTAGGG
GCCCTGGCCGCCGCAGGCGCCAATTACAGCCAGATGCCTGCAGGGTCCTTCATCACAGCC
GCCACCAACGGCCCTCCAGTGCTTGTGAAGAAGGAGAAGGAAATGGTGGGGGCATTGGTG
TCAGACGGGCTGGATCGGAAGGAGCCCCGAGCCGGGGAGGTGATCTGTATAGACGACTTG
Protein sequence predicted based on nucleotide sequence
MASPLRDEEEEEEEMVVSEEEEEEEEEGDEEEEEEVEAADEDDEEDDDEGVLGRGPGHDR GRDRHSPPGCHLFPPPPPPPPPLPPPPPPPPPDKDDIRLLPSALGVKKRKRGPKKQKENK PGKPRKRKKRDSEEEFGSERDEYREKSESGGSEYGTGPGRKRRRKHREKKEKKTKRRKKG EGDGGQKQVEQKSSATLLLTWGLEDVEHVFSEEDYHTLTNYKAFSQFMRPLIAKKNPKIP MSKMMTILGAKWREFSANNPFKGSAAAVAAAAAAAAAAVAEQVSAAVSSATPIAPSGPPA LPPPPAADIQPPPIRRAKTKEGKGPGHKRRSKSPRVPDGRKKLRGKKMAPLKIKLGLLGG KRKKGGSYVFQSDEGPEPEAEESDLDSGSVHSASGRPDGPVRTKKLKRGRPGRKKKKVLG CPAVAGEEEVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELDRAPEGKWSCP HCEKEGVQWEAKEEEEEYEEEGEEEGEKEEEDDHMEYCRVCKDGGELLCCDACISSYHIH CLNPPLPDIPNGEWLCPRCTCPVLKGRVQKILHWRWGEPPVAVPAPQQADGNPDVPPPRP LQGRSEREFFVKWVGLSYWHCSWAKELQLEIFHLVMYRNYQRKNDMDEPPPLDYGSGEDD GKSDKRKVKDPHYAEMEEKYYRFGIKPEWMTVHRIINHSVDKKGNYHYLVKWRDLPYDQS TWEEDEMNIPEYEEHKQSYWRHRELIMGEDPAQPRKYKKKKKELQGDGPPSSPTNDPTVK YETQPRFITATGGTLHMYQLEGLNWLRFSWAQGTDTILADEMGLGKTIQTIVFLYSLYKE GHTKGPFLVSAPLSTIINWEREFQMWAPKFYVVTYTGDKDSRAIIRENEFSFEDNAIKGG KKAFKMKREAQVKFHVLLTSYELITIDQAALGSIRWACLVVDEAHRLKNNQSKFFRVLNG YKIDHKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLG PHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKYILTRNFEALNSRGGGNQVSLLNI MMDLKKCCNHPYLFPVAAMESPKLPSGAYEGGALIKSSGKLMLLQKMLRKLKEQGHRVLI FSQMTKMLDLLEDFLDYEGYKYERIDGGITGALRQEAIDRFNAPGAQQFCFLLSTRAGGL GINLATADTVIIFDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRASVEERITQVAKRK MMLTHLVVRPGLGSKAGSMSKQELDDILKFGTEELFKDENEGENKEEDSSVIHYDNEAIA RLLDRNQDATEDTDVQNMNEYLSSFKVAQYVVREEDKIEEIEREIIKQEENVDPDYWEKL LRHHYEQQQEDLARNLGKGKRVRKQVNYNDAAQEDQDNQSEYSVGSEEEDEDFDERPEGR RQSKRQLRNEKDKPLPPLLARVGGNIEVLGFNTRQRKAFLNAVMRWGMPPQDAFTTQWLV RDLRGKTEKEFKAYVSLFMRHLCEPGADGSETFADGVPREGLSRQQVLTRIGVMSLVKKK VQEFEHINGRWSMPELMPDPSADSKRSSRASSPTKTSPTTPEASATNSPCTSKPATPAPS EKGEGIRTPLEKEEAENQEEKPEKNSRIGEKMETEADAPSPAPSLGERLEPRKIPLEDEV PGVPGEMEPEPGYRGDREKSATESTPGERGEEKPLDGQEHRERPEGETGDLGKREDVKGD RELRPGPRDEPRSNGRREEKTEKPRFMFNIADGGFTELHTLWQNEERAAISSGKLNEIWH RRHDYWLLAGIVLHGYARWQDIQNDAQFAIINEPFKTEANKGNFLEMKNKFLARRFKLLE QALVIEEQLRRAAYLNLSQEPAHPAMALHARFAEAECLAESHQHLSKESLAGNKPANAVL HKVLNQLEELLSDMKADVTRLPATLSRIPPIAARLQMSERSILSRLASKGTEPHPTPAYP PGPYATPPGYGAAFSAAPVGALAAAGANYSQMPAGSFITAATNGPPVLVKKEKEMVGALV SDGLDRKEPRAGEVICIDDL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
