Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-011.0086
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00820733
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4731nts
Open reading frame: 1 to 4731
ATGCATAACACAGCAGATGAGGTGGTTTTGTATCACAAATTAGCAGGTCCGTGGGGAAGG
TGTACAGGAGACTGTGGTCCCGGAGGAGTGCAGAGTCGGGCAGTGTGGTGTTTTCATGTT
GACGGGTGGACAAGTCACCTGTCTAACTGTGGTGAGAGCAACAGGCCTCCAAAGGAAAGA
AGTTGTTTCCGAGTTTGTGACTGGCACAGTGACCTCTTTCAGTGGGAGGTTTCTGACTGG
CACCACTGTGTGCTTGTGCCTTACGCTCGCGGTGAAGTCAAGCCTCGGACCGCAGAGTGT
GTGACGGCTCAGCATGGACTGCAGCACCGGATGGTGCGCTGCATTCAGAAGCTGAACCGA
ACTGTGGTTGCAAATGAAATATGCGAACACTTTGCCCTTCAGCCTCCTACAGAACAGGCT
TGCCTCATTCCTTGTCCCCGGGATTGTGTAGTATCTGAGTTCTTACCATGGTCCAACTGT
AGTAAGGGATGTGGGAAGAAATTGCAGCATAGAACTCGCGCGGTCATAGCTCCCCCTCTC
TTTGGTGGTTTGCAATGTCCAAATCTGACTGAGTCAAGAGCCTGTGATGCTCCCATTTCC
TGTCCTCTTGGGGAAGAGGAATATACATTTAGCCTTAAGGTTGGACCATGGAGTAAATGC
AGACTGCCTCATCTTAAAGAAATTAATCCAAGCGGAAGAACTGTTCTGGATTTTAACTCC
GATTCCAATGAGCGAGTCACCTTTAAACATCAAAGTTACAAAGCACATCATCATTCGAAG
TCTTGGGCAATAGAGATAGGTTATCAAACCCGGCAGGTTTCGTGTACAAGAAGTGATGGA
CAAAATGCTATGTTAAGCCTTTGCCTTCAAGATTCCTTCCCATTGACTGTTCAGTCCTGC
ATCATGCCCAAAGACTGTGAAACCTCCCAGTGGTCCTCCTGGAGCCCCTGCTCCAAGACA
TGCCGTTCAGGGAGTCTGTTGCCAGGATTTAGGAGCAGGAGCCGGAACGTGAAGCACATG
GCTATTGGAGGTGGAAAGGAGTGTCCTGAACTTCTTGAGAAAGAGGCCTGCATTGTTGAA
GGAGAACTTCTGCAGCAATGTCCCAGATATTCCTGGAGAACTTCTGAATGGAAAGAATGC
CAAGTCTCTCTGCTCCTGGAGCAGCAGGATCCCCACTGGCATGTGACGGGACCCGTGTGT
GGCGGTGGGATCCAGACCCGGGAGGTGTACTGTGCCCAGAGCGTACCAGCAGCTGCCGCA
CTGAGGGCCAAGGAAGTCTCTAGACCTGTGGAAAAGGCATTATGTGTGGGACCCGCCCCG
TTGCCCTCTCAGCTCTGCAATATCCCTTGCTCTACGGACTGCATAGTATCTTCCTGGTCA
GCCTGGGGCCTGTGCATCCATGAAAACTGTCATGATCCTCAGGGCAAAAAAGGATTTAGA
ACGAGGCAGCGCCATGTCCTCATGGAATCTACAGGGCCTGCAGGGCATTGCCCTCATTTG
GTGGAGTCTGTTCCTTGTGAGGATCCAATGTGCTACCGATGGCTGGCATCAGAAGGGATC
TGTTTCCCTGATCATGGAAAATGTGGCCTGGGACATCGTATTCTGAAGGCCGTCTGCCAG
AATGACCGCGGAGAAGATGTATCAGGGAGTCTTTGCCCAGTTCCCCCTCCCCCTGAGAGG
AAGTCTTGTGAAATTCCCTGCAGAATGGACTGTGTGCTGAGCGAGTGGACGGAGTGGTCA
TCCTGTTCCCAGTCCTGTTCAAATAAAAACTCAGATGGGAAACAGACCAGGTCAAGAACT
ATCCTGGCACTGGCTGGGGAAGGTGGAAAGCCATGTCCCCCTAGTCAGGCTCTCCAAGAG
CATCGTTTGTGTAATGACCATTCCTGTATGCAGCTTCACTGGGAGACATCGCCTTGGGGC
CCTTGTTCTGAGGACACATTGGTAACTGCCCTTAATGCAACCATTGGCTGGAATGGAGAA
GCCACGTGTGGTGTAGGCATTCAGACTCGGAGAGTCTTCTGTGTCAAGAGTCACGTGGGA
CAAGTAATGACCAAAAGATGTCCAGATTCTACTCGACCTGAAACTGTGCGCCCCTGTTTT
CTCCCATGCAAAAAAGACTGTATTGTGACTGCTTTCAGTGAGTGGACACCCTGCCCAAGG
ATGTGCCAAGCAGGAAATGCCACCGTAAAACAGTCTCGATACAGAATCATCATCCAAGAA
GCAGCCAATGGAGGCCAGGAATGCCCAGATACCTTATATGAGGAGAGAGAGTGTGAAGAT
GTTTCCTTGTGTCCTGTATATCGGTGGAAGCCACAGAAATGGAGCCCTTGCATCTTAGTG
CCAGAGTCTGTCTGGCAGGGAATAACGGGCAGCAGTGAAGCCTGTGGAAAGGGGTTACAA
ACAAGGGCTGTCTCATGCATCTCTGATGACAACCGGTCAGCAGAAATGATGGAATGCCTC
AAGCAGACAAACGGCATGCCTCTCCTTGTGCAAGAATGCACAGTCCCATGTCGAGAAGAC
TGCACCTTCACTGCTTGGTCCAAGTTTACGCCCTGCTCTACGAACTGTGAAGCCACAAAA
AGCAGGCGGCGACAGCTTACAGGGAAAAGCAGAAAGAAGGAGAAATGCCAGGATTCTGAC
CTTTACCCTCTAGTGGAGACAGAACTATGTCCTTGTGATGAATTTATATCCCAACCTTAT
GGAAACTGGTCAGATTGCATTCTTCCAGAAGGCAGAAGGGAGCCTCACAGAGGACTGCGG
GTACAAGCAGACAGCAAAGAATGTGGAGAAGGCCTGCGCTTTCGAGCAGTAGCTTGTTCT
GATAAAAATGGAAGACCTGTTGACCCCTCCTTCTGCAGCAGCTCCGGTTACATTCAAGAA
AAATGTGTCATTCCCTGCCCATTTGATTGCAAGTTAAGCGATTGGTCTAGTTGGGGGTCT
TGCAGTTCATCTTGTGGAATTGGAGTGAGGATTCGATCCAAATGGCTAAAAGAAAAACCT
TACAATGGAGGACGACCATGTCCCAAACTGGATCTCAAGAATCAGGCTCAGGTACATGAG
GCAGTCCCATGTTACAGTGAGTGCAATCAGTATTCCTGGGTTGTGGAACACTGGTCTTCA
TGCAAAATCAACAATGAGCTGAGGTCCCTGCGGTGTGGAGGAGGAACACAATCTAGGAAA
ATCAGATGTGTGAATACTGCGGATGGTGAAGGTGGAGCAGTGGATAGCAACCTGTGCAAC
CAGGATGAAATTCCCCCAGAAACCCAGTCCTGTTCTCTTATGTGTCCCAATGAGTGTGTC
ATGTCTGAGTGGGGACTTTGGAGCAAATGCCCACAGTCATGCGATCCCCACACAATGCAG
AGAAGAACTCGCCACCTCCTAAGACCATCACTGAACTCAAGGACTTGTGCTGAAGACTCA
CAGGTGCAGCCTTGCCTCCTGAATGAAAATTGCTTCCAGTTCCAGTACAATCTAACAGAG
TGGAGCACATGCCAGCTGAGTGAAAACGCACCCTGTGGTCAAGGCGTCAGGACCCGCCTG
CTAAGCTGTGTGTGCAGTGATGGCAAGCCAGTCAGCATGGACCAATGTGAGCAGCATAAT
TTGGAGAAGCCCCAGAGAATGAGCATTCCCTGCTTGGTGGAATGCGTGGTCAACTGTCAG
CTCTCAGGGTGGACGGCTTGGACAGAGTGTTCACAGACCTGTGGCCATGGAGGTCGAATG
AGCCGGACTCGATTTATCATTATGCCAACCCAAGGAGAAGGACGGCCATGCCCCACAGAG
CTTACCCAGGAGAAAACCTGCCCAGTGACCCCCTGCTACAGTTGGGTCCTGGGCAACTGG
TCTGCATGTAAATTGGAGGGTGGAGACTGTGGGGAAGGAGTTCAGATCCGCAGCCTTTCC
TGCATGGTCCACAGTGGTTCAATATCTCATGCAGCTGGACGTGTCGAGGATGCACTGTGT
GGAGAAATGCCCTTTCAGGACAGTATCCTGAAGCAGCTGTGTTCCGTGCCTTGCCCAGGA
GACTGCCATTTAACAGAATGGTCAGAGTGGAGCACATGTGAATTAACCTGCATTGATGGA
AGAAGCTTTGAGACTGTGGGCCGCCAGTCTAGATCAAGGACTTTTATAATTCAGTCTTTT
GAGAACCAAGACAGCTGCCCCCAACAGGTTCTAGAAACACGCCCTTGTACAGGAGGCAAA
TGTTATCACTACACATGGAAAGCAAGTCTTTGGAACAATAACGAACGAACTGTATGGTGC
CAGCGTTCAGATGGCGTTAATGTCACAGGAGGCTGCTCCCCTCAGGCCCGTCCTGCTGCC
ATTCGGCAGTGCATTCCAGCCTGCAGAAAACCTTTCTCCTACTGTACACAGGGTGGAGTC
TGTGGTTGTGAGAAGGGCTATACAGAGATAATGAAATCCAATGGTTTCCTGGATTACTGC
ATGAAAGTACCAGGCTCCGAGGATAAAAAAGCCGATGTGAAAAACCTTTCTGGGAAAAAC
AGACCTGTGAATTCAAAAATACATGATATTTTTAAAGGATGGTCCCTTCAACCACTTGAT
CCAGATGGCCGAGTAAAAATTTGGGTTTATGGCGTGTCTGGTGGCGCTTTTCTCATCATG
ATTTTCCTAATATTTACTTCCTACCTTGTTTGCAAGAAGCCAAAACCACATCAAAGCACA
CCTCCCCAACAGAAGCCTCTGACCTTAGCCTACGATGGAGACTTAGACATG
Protein sequence predicted based on nucleotide sequence
MHNTADEVVLYHKLAGPWGRCTGDCGPGGVQSRAVWCFHVDGWTSHLSNCGESNRPPKER SCFRVCDWHSDLFQWEVSDWHHCVLVPYARGEVKPRTAECVTAQHGLQHRMVRCIQKLNR TVVANEICEHFALQPPTEQACLIPCPRDCVVSEFLPWSNCSKGCGKKLQHRTRAVIAPPL FGGLQCPNLTESRACDAPISCPLGEEEYTFSLKVGPWSKCRLPHLKEINPSGRTVLDFNS DSNERVTFKHQSYKAHHHSKSWAIEIGYQTRQVSCTRSDGQNAMLSLCLQDSFPLTVQSC IMPKDCETSQWSSWSPCSKTCRSGSLLPGFRSRSRNVKHMAIGGGKECPELLEKEACIVE GELLQQCPRYSWRTSEWKECQVSLLLEQQDPHWHVTGPVCGGGIQTREVYCAQSVPAAAA LRAKEVSRPVEKALCVGPAPLPSQLCNIPCSTDCIVSSWSAWGLCIHENCHDPQGKKGFR TRQRHVLMESTGPAGHCPHLVESVPCEDPMCYRWLASEGICFPDHGKCGLGHRILKAVCQ NDRGEDVSGSLCPVPPPPERKSCEIPCRMDCVLSEWTEWSSCSQSCSNKNSDGKQTRSRT ILALAGEGGKPCPPSQALQEHRLCNDHSCMQLHWETSPWGPCSEDTLVTALNATIGWNGE ATCGVGIQTRRVFCVKSHVGQVMTKRCPDSTRPETVRPCFLPCKKDCIVTAFSEWTPCPR MCQAGNATVKQSRYRIIIQEAANGGQECPDTLYEERECEDVSLCPVYRWKPQKWSPCILV PESVWQGITGSSEACGKGLQTRAVSCISDDNRSAEMMECLKQTNGMPLLVQECTVPCRED CTFTAWSKFTPCSTNCEATKSRRRQLTGKSRKKEKCQDSDLYPLVETELCPCDEFISQPY GNWSDCILPEGRREPHRGLRVQADSKECGEGLRFRAVACSDKNGRPVDPSFCSSSGYIQE KCVIPCPFDCKLSDWSSWGSCSSSCGIGVRIRSKWLKEKPYNGGRPCPKLDLKNQAQVHE AVPCYSECNQYSWVVEHWSSCKINNELRSLRCGGGTQSRKIRCVNTADGEGGAVDSNLCN QDEIPPETQSCSLMCPNECVMSEWGLWSKCPQSCDPHTMQRRTRHLLRPSLNSRTCAEDS QVQPCLLNENCFQFQYNLTEWSTCQLSENAPCGQGVRTRLLSCVCSDGKPVSMDQCEQHN LEKPQRMSIPCLVECVVNCQLSGWTAWTECSQTCGHGGRMSRTRFIIMPTQGEGRPCPTE LTQEKTCPVTPCYSWVLGNWSACKLEGGDCGEGVQIRSLSCMVHSGSISHAAGRVEDALC GEMPFQDSILKQLCSVPCPGDCHLTEWSEWSTCELTCIDGRSFETVGRQSRSRTFIIQSF ENQDSCPQQVLETRPCTGGKCYHYTWKASLWNNNERTVWCQRSDGVNVTGGCSPQARPAA IRQCIPACRKPFSYCTQGGVCGCEKGYTEIMKSNGFLDYCMKVPGSEDKKADVKNLSGKN RPVNSKIHDIFKGWSLQPLDPDGRVKIWVYGVSGGAFLIMIFLIFTSYLVCKKPKPHQST PPQQKPLTLAYDGDLDM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
