Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0005
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00820802
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4836nts
Open reading frame: 1 to 4836
ATGCGGAAAAGATGGGCCTGCTGGAGCGGAAGTGACGCTCCCGGAGGCTGTGGCGGAGGC
TGCGGGAGAAGGAGAAGGAGGAGTAGAAGGAAGAGGGCCGCCTCCGAAGAAAGGAGAATG
GCGTTCAGTAAAGGATTCCGGATCTATCACAAATTGGATCCCCCACCTTTCAGCCTCATA
GTGGAAACTAGGCATAAGGAAGAATGTCTCATGTTCGAGTCTGGGGCTGTCGCTGTGCTC
TCATCTGCAGAAAAAGAGGCAATCAAGGGTACATACTCCAAAGTACTGGATGCATATGGA
CTCTTAGGTGTTCTGCGGTTAAATCTTGGTGATACTATGTTACATTATCTGGTCCTAGTC
ACTGGATGTATGTCTGTTGGAAAAATTCAAGAATCTGAAGTTTTCCGAGTTACTTCCACT
GAGTTTATATCACTGAGAATCGATTCTTCAGATGAGGATCGCATTTCAGAAGTGCGGAAA
GTTTTGAATTCAGGAAACTTTTATTTTGCATGGTCTGCATCTGGCATCAGTTTAGATTTG
AGTCTTAATGCGCATCGTAGCATGCAAGAACAGACAACCGATAATAGATTTTTCTGGAAT
CAGTCTTTGCATTTGCATCTCAAACACTATGGCGTGAATTGTGATGACTGGTTATTACGT
CTTATGTGTGGAGGAGTAGAAATCAGAACAATTTATGCTGCTCATAAACAGGCGAAGGCT
TGCCTCATTTCAAGATTAAGCTGTGAACGAGCTGGGACCAGGTTTAATGTCCGGGGAACA
AATGATGATGGTCATGTTGCCAATTTTGTAGAAACAGAACAGGTTGTGTACTTAGATGAC
TCAGTTTCTTCCTTCATACAAATCCGAGGATCTGTTCCATTGTTCTGGGAGCAACCAGGG
TTGCAAGTGGGATCTCATCGTGTCCGTATGTCAAGGGGATTTGAAGCCAATGCACCCGCT
TTTGACAGGCATTTTAGGACACTTAAGAACTTATATGGTAAACAAATAATAGTAAATCTG
CTTGGCTCTAAGGAAGGTGAACATATGCTGAGTAAAGCTTTCCAGAGTCATTTGAAAGCT
TCTGAACATGCTGCTGATATCCAGATGGTGAATTTTGACTATCATCAAATGGTGAAGGGA
GGAAAGGCAGAAAAATTACATAGTGTTCTGAAACCTCAGGTCCAGAAGTTTCTAGATTAT
GGATTTTTTTATTTCAATGGAAGTGAAGTTCAAAGATGCCAGAGTGGTACAGTTCGAACA
AACTGCTTGGATTGTCTTGATAGAACAAATAGTGTGCAGGCATTTCTTGGCTTAGAGATG
CTAGCTAAACAGTTGGAAGCTCTTGGTTTAGCTGAAAAGCCTCAGTTGGTGACTCGCTTT
CAAGAAGTTTTTCGGTCCATGTGGTCCGTGAATGGTGATTCAATCAGTAAGATATATGCA
GGAACTGGAGCTCTTGAAGGGAAAGCGAAGTTAAAAGATGGTGCTCGCTCTGTTACCCGA
ACAATTCAGAATAACTTCTTTGACAGCTCCAAGCAAGAGGCCATTGATGTTTTGCTACTG
GGAAATACTCTGAATAGTGATTTAGCTGACAAAGCTCGAGCACTTTTAACTACTGGAAGT
TTGCGTGTTTCTGAGCAGACATTACAGTCAGCATCTTCTAAAGTACTAAAGAGCATGTGT
GAGAATTTCTACAAATATTCAAAGCCTAAGAAAATTCGAGTATGTGTCGGAACCTGGAAT
GTGAATGGTGGGAAGCAATTTCGCAGCATAGCTTTTAAGAATCAGACACTCACTGACTGG
CTTCTTGATGCACCCAAGTTAGCTGGCATCCAGGAGTTTCAAGATAAAAGAAGTAAGCCA
ACTGATATATTTGCAATTGGTTTTGAAGAAATGGTAGAACTGAATGCTGGAAACATTGTG
AGTGCAAGCACAACAAATCAGAAGCTCTGGGCTGTAGAACTTCAGAAGACAATCTCCAGA
GACAACAAGTATGTGCTGCTGGCTTCTGAACAGTTGGTGGGCGTCTGTTTGTTTGTTTTT
ATCAGACCACAGCATGCTCCTTTTATCAGGGATGTTGCAGTTGATACTGTGAAGACTGGA
ATGGGAGGTGCAACTGGAAATAAGGGAGCAGTTGCAATCCGAATGCTCTTCCATACAACC
AGCCTTTGCTTCGTCTGTAGCCACTTTGCTGCAGGGCAGTCACAAGTCAAAGAAAGAAAT
GAAGATTTTATAGAAATAGCACGAAAATTGAGCTTTCCCATGGGAAGGATGCTATTTTCC
CATGACTATGTATTTTGGTGTGGTGATTTCAACTATCGAATCGATCTCCCTAACGAAGAA
GTTAAAGAGCTGATAAGACAGCAAAATTGGGATTCTCTTATAGCAGGAGATCAACTTATC
AATCAGAAAAATGCTGGACAGGTTTTTAGAGGATTTTTAGAAGGAAAGGTAACCTTTGCT
CCGACATATAAGTATGACTTGTTTTCTGACGACTATGACACCAGTGAAAAGTGCCGCACC
CCTGCCTGGACAGACCGGGTCCTTTGGAGAAGGAGGAAATGGCCTTTTGATAGATCAGCT
GAAGATCTAGATCTTCTAAATGCTAGTTTTCAAGATGAAAGCAAAATTCTGTACACGTGG
ACTCCAGGCACTTTGCTGCACTATGGAAGAGCTGAGCTGAAGACTTCTGACCACAGGCCT
GTCGTTGCCCTGATTGATATAGATATATTTGAAGTTGAAGCTGAAGAGAGGCAAAACATT
TATAAAGAAGTAATTGCAGTTCAGGGTCCACCAGATGGTACCGTATTAGTCTCAATCAAA
AGTTCTTTGCCCGAAAACAATTTTTTTGATGATGCCTTGATTGATGAGCTGCTGCAGCAG
TTCGCAAGTTTTGGTGAAGTTATACTTATCAGATTTGTAGAAGATAAAATGTGGGTTACA
TTTTTGGAGGGAAGCTCTGCCTTGAATGTTCTCAGCCTGAATGGTAAAGAGTTGTTGAAT
CGGACTATAACCATTGCTTTGAAAAGTCCAGACTGGATCAAAAATTTGGAAGAAGAAATG
AGCTTAGAGAAAATTAGCATTGCATTGCCATCATCAACAAGCTCCACCCTGCTTGGTGAA
GATGCAGAGGTTGCAGCAGATTTTGATATGGAAGGTGATGTTGATGACTATAGTGCTGAA
GTGGAGGAACTTCTTCCTCAGCATCTCCAGCCATCTTCAAGTTCCGGCCTTGGTACTTCC
CCCAGCTCTTCACCCCGAACTAGTCCCTGCCAGTCACCTACAATATCAGAGGGTCCTGTA
CCTTCCCTTCCCATCAGACCAAGCCGAGCACCGTCAAGAACTCCTGGGCCTCCCAGTGCA
CAGAGTTCTCCTATTGACGCGCAGCCAGCAACGCCTCTTCCGCAGAAAGACCCCGCTCAG
CCCTTGGAGCCTAAGAGGCCGCCTCCGCCTCGACCTGTTGCCCCTCCTACAAGACCTGCT
CCTCCACAGAGGCCTCCTCCTCCTTCAGGAGCTAGAAGTCCTGCACCCACTAGAAAGGAA
TTTGGAGGTATTGGAGCCCCTCCCAGTCCTGGGGTAGCTAGGAGAGAGATGGAAGCACCC
AAAAGTCCTGGAACAACAAGGAAAGATAATATAGGACGCAGTCAGCCTTCACCTCAAGCA
GGACTTGCAGGCCCAGGACCTGCTGGATACAGTACAGCCAGACCGACGATTCCTCCTCGT
GCTGGAGTTATCAGTGCCCCACAGAGCCACGCGCGGGCATCTGCTGGAAGACTGACTCCT
GAAAGCCAAAGCAAAACATCAGAAACGTCGAAAGGTTCAACTTTCCTTCCTGAACCACTG
AAGCCTCAGGCTGCTTTTCCTCCGCAGTCTTCTTTGCCCCCGCCTGCTCAAAGGTTGCAA
GAGCCTCTTGTCCCTGTGGCAGCACCTATGCCTCAGTCTGGCCCCCAGCCAAATTTGGAA
ACCCCACCACAACCACCACCTCGAAGCAGGTCATCCCATAGCTTGCCTTCAGAAGCTTCC
TCACAACCGCAAGTAAAAACAAATGGAATCTCTGATGGCAAAAGAGAATCACCATTAAAG
ATTGACCCATTTGAAGATCTGTCATTTAATCTCCTTGCTGTATCAAAGGCTCAGCTATCT
GTTCAAACGTCACCTGTTCCCACCCCAGACCCAAAGAGGTTGATTCAGTTGCCTTCTGCA
ACGCAAAGTAATGTTTTGAGTTCTGTAAGTTGCATGCCAACAATGCCTCCAATTCCAGCT
CGGAGTCAATCCCAGGAAAATATGCGAAGTTCTCCAAACCCATTTATTACTGGCTTGACC
AGGACAAATCCTTTCAGTGACAGGACTGCTGCTCCTGGAAACCCATTTAGAGCCAAGTCT
GAAGAATCAGAGGCAACTTCATGGTTCTCCAAAGAAGAGCCCGTTACTATCAGTCCTTTC
CCTTCTCTGCAGCCTCTTGGTCATAACAAAAGCAGGGCTTCATCTTCACTTGATGGCTTT
AAGGACAGTTTTGATCTACAGGGCCAGTCTACATTAAAAATTAGCAACCCGAAAGGATGG
GTAACCTTCGAGGAAGAAGAGGATTTTGGTGTGAAAGGGAAGTCAAAGTCAGCTTGTTCA
GACTTACTGGGTAATCAGCCAAGTTCATTTTCTGGCTCCAACCTGACATTGAATGATGAC
TGGAATAAAGGTACAAATGTCTCCTTCTGTGTGTTGCCGTCAAGAAGACCTCCTCCACCT
CCTGTCCCTCTGCTCCCGCCCGGCACCAGCCCTCCAGTAGATCCTTTCACGACCTTGGCC
TCTAAGGCTTCACCCACACTGGACTTTACAGAAAGA
Protein sequence predicted based on nucleotide sequence
MRKRWACWSGSDAPGGCGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLI VETRHKEECLMFESGAVAVLSSAEKEAIKGTYSKVLDAYGLLGVLRLNLGDTMLHYLVLV TGCMSVGKIQESEVFRVTSTEFISLRIDSSDEDRISEVRKVLNSGNFYFAWSASGISLDL SLNAHRSMQEQTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKA CLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYLDDSVSSFIQIRGSVPLFWEQPG LQVGSHRVRMSRGFEANAPAFDRHFRTLKNLYGKQIIVNLLGSKEGEHMLSKAFQSHLKA SEHAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFNGSEVQRCQSGTVRT NCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYA GTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGS LRVSEQTLQSASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDW LLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVSASTTNQKLWAVELQKTISR DNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTT SLCFVCSHFAAGQSQVKERNEDFIEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEE VKELIRQQNWDSLIAGDQLINQKNAGQVFRGFLEGKVTFAPTYKYDLFSDDYDTSEKCRT PAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRP VVALIDIDIFEVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQQ FASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEM SLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTS PSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSAQSSPIDAQPATPLPQKDPAQ PLEPKRPPPPRPVAPPTRPAPPQRPPPPSGARSPAPTRKEFGGIGAPPSPGVARREMEAP KSPGTTRKDNIGRSQPSPQAGLAGPGPAGYSTARPTIPPRAGVISAPQSHARASAGRLTP ESQSKTSETSKGSTFLPEPLKPQAAFPPQSSLPPPAQRLQEPLVPVAAPMPQSGPQPNLE TPPQPPPRSRSSHSLPSEASSQPQVKTNGISDGKRESPLKIDPFEDLSFNLLAVSKAQLS VQTSPVPTPDPKRLIQLPSATQSNVLSSVSCMPTMPPIPARSQSQENMRSSPNPFITGLT RTNPFSDRTAAPGNPFRAKSEESEATSWFSKEEPVTISPFPSLQPLGHNKSRASSSLDGF KDSFDLQGQSTLKISNPKGWVTFEEEEDFGVKGKSKSACSDLLGNQPSSFSGSNLTLNDD WNKGTNVSFCVLPSRRPPPPPVPLLPPGTSPPVDPFTTLASKASPTLDFTER
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
