Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
PONG-001.0033
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00820805
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5931nts
Open reading frame: 1 to 5931
ATGTCGGAATCTGAAGGCGGGAAAGATACCACCCCAGAGCCCAGTCCAGCCAATGGTGCA
GGCCCAGGACCCGAATGGGGGCTGTGCCCCGGGCCCCCAGCTGTTGAAGGTGAAAGCAGT
GGAGCATCAGGACTGGGGACCCCTAAGCGAAGAAACCAGCACAGCAAGCACAAGACAGTG
GCCGTGGCCAGTGCCCAGAGGTCACCTCGGGCCCTCTTCTGCCTTACCCTGGCCAATCCT
TTGCGACGGTCTTGCATCAGCATCGTGGAGTGGAAGCCCTTCGACATCCTCATCCTGCTG
ACCATCTTTGCCAACTGCGTGGCCCTGGGAGTTTACATCCCCTTCCCTGAGGACGACTCC
AACACTGCCAACCATAACCTGGAGCAAGTGGAGTACGTATTCCTGGTCATTTTCACTGTG
GAGACGGTGCTCAAGATCGTGGCCTACGGGCTGGTGCTGCACCCTAGCGCCTACATCCGC
AATGGCTGGAACCTACTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTTCTTCTG
GAGCAGGGCCCTGGACGTCCAGGCGATGCCCCGCACACAGGGGGAAAGCCAGGCGGATTC
GATGTTAAGGCCTTGAGGGCGTTTCGGGTGCTGCGGCCACTGAGGCTGGTTTCTGGGGTC
CCGAGCCTGCACATAGTGCTCAATTCCATTATGAAGGCTCTGGTGCCCCTGCTGCACATT
GCACTGCTCGTGCTCTTCGTCATCATCATTTATGCCATCATTGGGCTCGAGCTGTTCCTT
GGAAGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAAGCGGAAGAGGACCCA
TCTCCCTGTGCGTCATCGGGATCAGGCCGTGCGTGCACGCTGAACCAGACTGAGTGCCGC
GGACGCTGGCCAGGGCCCAATGGAGGCATCACAAACTTTGACAACTTCTTCTTCGCCATG
CTGACAGTCTTCCAGTGTGTCACCATGGAAGGCTGGACCGATGTGCTCTACTGGATGCAA
GATGCCATGGGGTATGAACTGCCCTGGGTCTACTTTGTGAGCCTTGTCATCTTTGGGTCC
TTCTTCGTCCTGAACCTTGTGCTTGGCGTCCTGAGTGGGGAGTTCTCCAAGGAGAGAGAG
AAAGCGAAAGCTCGCGGGGACTTCCAGAAGCAGCGGGAGAAGCAGCAGATGGAGGAAGAC
CTGCGGGGCTACCTGGACTGGATCACTCAAGCCGAAGAGCTGGACATGGAGGACCCCTCC
GCCGATGACAACCTTGGTTCTATGGCTGAAGAGGGCCGGGCGGGCCATCGGCCACAGCTG
GCCGAGCTGACCAATAGGAGGCGGGGACGTTTGCGCTGGTTCAGTCATTCTACTCGCTCC
ACACACTCCACCAGCAGCCATGCCAGTCTCCCAGCCAGTGACACCGGTTCCATGACAGAG
ACACAAGGCGATGAGGATGAGGAGGAGGGGGCTCTGGCCAGCTGTACACGCTGCCTAAAC
AAGATCATGAAAACCAGAGTCTGCCGACGCCTCCGCCGAGCCAACCGCGTCCTTCGGGCA
CGCTGCCGTCGGGCAGTGAAGTCCAATGCCTGCTACTGGGCTGTCCTGTTGCTCGTCTTC
CTCAACACGTTGACCATCGCCTCAGAGCACCACGGGCAGCCTGTGTGGCTCACCCAGATC
CAGGAGTATGCCAACAAAGTGCTGCTCTGTCTGTTCACGGTGGAGATGCTTCTGAAATTG
TACGGTCTGGGCCCCTCTGCCTATGTGTCTTCCTTCTTCAACCGCTTTGACTGCTTTGTG
GTCTGTGGGGGCATCCTAGAGACAACCTTGGTGGAGGTGGGCGCCATGCAGCCCTTGGGC
ATCTCAGTGCTCCGATGTGTGCGCCTCCTAAGGATCTTTAAGGTCACCAGACACTGGGCT
TCTCTGAGCAATCTGGTGGCATCCTTGCTCAATTCAATGAAATCAATCGCATCCTTGCTG
CTTCTGCTCTTCCTCTTCATCATTATCTTCTCCCTGCTTGGCATGCAGCTGTTTGGGGGC
AAGTTCAACTTTGACCAGACCCACACCAAGCGAAGCACCTTTGACACGTTCCCCCAGGCC
CTCCTCACTGTCTTTCAGATCCTGACAGGTGAGGACTGGAACGTGGTCATGTATGATGGT
ATCATGGCATATGGTGGCCCCTTCTTCCCAGGAATGTTGGTGTGCATCTATTTCATCATT
CTCTTCATCTGTGGCAACTACATCCTGTTGAACGTGTTTCTTGCCATTGCTGTGGACAAC
CTGGCTAGTGGAGATGCAGGCACTGCCAAGGACAAGGGCGGGGAGAAGAGCAATGAGAAG
GATCTCCCACAGGAGAATGAAGGCCTGGTGCCTGGTGTGGAGAAAGAGGAAGAGGAGGGT
GCAAGGAGGGAAGGAGCAGACATGGAGGAGGAGGAGGAGGAGGAAGAAGAGGAAGAAGAG
GAAGAAGAGGAAGAGGGTGCAGGGGGTGTGGAACTCCTGCAGGAAGTTGTACCCAAGGAG
AAGGTGGTACCCATCCCTGAGGGCAGCGCCTTCTTCTGCCTCAGCCAAACCAACCCGCTC
AGGAAGGGCTGCCACACCCTCATCCACCATCATGTCTTCACCAATCTTATCCTGGTGTTC
ATCATCCTCAGCAGTGTGTCCCTGGCCGCTGAGGACCCCATCCGAGCCCACTCCTTCCGC
AACCATATTCTGGGTTACTTCGATTATGCCTTCACCTCCATTTTCACTGTGGAGATTCTA
CTAAAGATGACAGTGTTTGGGGCCTTCCTCCACCGCGGCTCCTTCTGCCGTAGCTGGTTT
AATATGTTGGATCTGCTGGTGGTCAGTGTGTCCCTCATCTCCTTTGGCATCCACTCCAGC
GCCATCTCGGTGGTGAAGATTCTGCGAGTACTCCGAGTACTGCGGCCCCTCCGAGCCATC
AACAGGGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTATTTGTGGCCATCCGGACCATC
GGGAACATCATGATTGTCACCACACTTCTGCAATTTATGTTCGCCTGCATCGGGGTGCAG
CTCTTCAAGGGGAAATTCTACACATGCACGGACGAGGCCAAACACACCCCTCAAGAATGC
AAGGGCTCCTTCCTGGTATACCCAGATGGAGACGTTTCACGGCCCCTGGTCAGGGAGCGG
CTCTGGGTCAACAGCGATTTCAACTTTGACAATGTCCTTTCAGCCATGATGGCCCTGTTC
ACTGTCTCCACCTTTGAAGGCTGGCCTGCACTGCTATACAAGGCCATCGATGCATATGCA
GAGGACCACGGCCCCATCTATAATTACCGTGTGGAGATCTCAGTGTTCTTCATTGTCTAC
ATCATCATCATTGCGTTCTTCATGATGAACATCTTCGTGGGCTTCGTCATCATCACTTTC
CGTGCCCAGGGCGAGCAGGAGTACCAAAACTGTGAGCTGGACAAGAACCAGCGTCAATGT
GTGGAATATGCCCTTAAGGCCCAGCCACTCCGCCGTTATATCCCCAAGAACCCGCATCAG
TATCGTGTGTGGGCCACTGTGAACTCTGCTGCCTTTGAGTACCTTATGTTCCTGCTCATC
CTGCTCAACACAGTTGCCCTGGCCATGCAGCACTATGAGCAGACTGCTCCCTTCAACTAT
GCCATGGACATCCTCAACATGGTCTTCACTGGCCTCTTCACTATTGAGATGGTGCTCAAA
ATCATCGCCTTCAAGCCCAAGCATTACTTCACTGATGCCTGGAACACGTTTGACGCTCTT
ATTGTGGTGGGCAGCATAGTGGATATTGCCGTCACTGAAGTCAATAATGGTGGCCACCTT
GGCGAGAGCTCTGAGGACAGCTCCCGCATTTCCATTACCTTCTTTCGGCTCTTCCGAGTT
ATGCGGCTGGTCAAGCTTCTCAGTAAGGGTGAAGGGATTCGCACATTGCTCTGGACATTC
ATCAAGTCCTTTCAGGCCTTGCCCTATGTGGCACTTCTCATCGCAATGATATTCTTCATC
TATGCCGTCATTGGCATGCAGATGTTCGGCAAGGTGGCTCTTCAGGATGGCACACAGATA
AACCGAAACAACAACTTCCAGACCTTTCCACAGGCTGTGCTGCTTCTGTTCAGGTGTGCC
ACTGGTGAGGCATGGCAGGAGATAATGCTTGCCAGCCTTCCCGGAAATCGGTGTGATCCT
GAGTCTGACTTCGGCCCTGGTGAAGAGTTTACCTGTGGTAGCAATTTTGCCATCGCCTAT
TTCATCAGCTTCTTCATGCTCTGTGCCTTCCTGATCATAAATCTCTTTGTGGCTGTGATC
ATGGACAACTTTGATTATCTCACCAGAGATTGGTCTATCCTGGGCCCCCATCACCTTGAT
GAATTCAAGAGAATCTGGTCTGAATATGACCCTGGCGCCAAGGGCCGCATCAAACACTTG
GATGTGGTTGCCCTGCTGAGACGTATCCAGCCCCCTCTGGGATTTGGCAAGCTGTGCCCA
CACCGAGTGGCCTGCAAGAGACTTGTGGCAATGAACATGCCCCTCAACTCAGATGGGACG
GTGACATTCAACGCCACACTCTTTGCCCTGGTGCGGACATCACTGAAGATCAAAACAGAA
GGGAACCTGGAGCAAGCCAACCAGGAGCTGCGGATTGTCATCAAAAAGATCTGGAAGCGG
ATGAAACAGAAGCTGCTAGATGAGGTCATCCCCCCACCAGACGAGGAGGAGGTCACCGTG
GGCAAATTCTACGCCACATTTCTGATCCAGGACTATTTCCGCAAGTTCCGGCGGAGGAAA
GAAAAAGGGCTTCTAGGCAACGACGCCGCCCCTAGCACCTCTTCCGCCCTTCAGGCTGGT
CTGCGGAGCCTGCAGGACTTGGGTCCTGAGATGCGGCAGGCCCTCACCTGTGACACAGAG
GAGGAGGAAGAAGAGGGGCAGGAGGGAGTGGAGGAGGAAGATGAAAAGGACTTGGAAACT
AACAAAGCCACGATGGTGTCCCAGCCCTCAGCTCGCAGGGGCTCCGGGATTTCTGTGTCT
CTGCCTGTCGGGGACAGACTTCCAGATTCACTCTCTTTTGGGCCCAGTGATGATGACAGG
GGGACTCCCACCTCCAGTCAGCCCAGTGTGCCACAGGCTGGATCCAACACCCACAGAAGA
GGCTCCGGCGCTCTGATTTTCACCATCCCAGAAGAAGGAAATTCACAGCCCAAGGGAACC
AAAGGGCAAAACAAGCAAGATGAGGATGAGGAAGTCCCTGATCGGCTTTCCTACCTAGAT
GAGCAGGCAGGGACTCCCCCGTGCTCAGTCCTTTTGCCACCTCACAGAGCTCAGAGATAC
ATGGATGGGCACCTGGTACCTCGCCGACGTCTGCTGCCCCCCACCCCTGCAGGTCGGAAG
CCCTCCTTCACCATTCAGTGTCTGCAGCGCCAGGGCAGTTGTGAGGATTTACCCATCCCA
GGCACCTATCATCGTGGGCGAAATTCCGGGCCCAATAGGGCTCAGGGTTCCTGGGCAACA
CCACCTCAGAGGGGTCGGCTCCTGTATGCCCCTCTGTTGTTGGTGGAAGAGGGCGCAGCG
GGGGAGGGGTACCTCGGCAGATCCAGTGGACCACTGCGCACCTTCACCTGTCTGCATGTG
CCTGGAACCCATTCGGACCCCAGCCATGGGAAGAGGGGCAGTGCCGACAGCTTGGTGGAG
GCTGTGCTTATCTCAGAGGGTCTGGGCCTCTTTGCTCGGGACCCACGTTTCGTGGCCCTG
GCCAAGCAGGAGATTGCAGATGCGTGTCGCCTGACGCTGGATGAGATGGACAATGCTGCC
AGTGACCTGCTGGCACAGGGAACCAGCTCTCTCTATAGCGACGAGGAGTCCATCCTCTCC
CGGTTCGATGAGGAGGACTTGGGAGACGAGATGGCCTGCGTCCACGCCCTC
Protein sequence predicted based on nucleotide sequence
MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTV AVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDS NTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLL EQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHI ALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECR GRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGS FFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPS ADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTE TQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVF LNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFV VCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLL LLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDG IMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEK DLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKE KVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFR NHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSS AISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQ LFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALF TVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITF RAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLI LLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDAL IVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTF IKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCA TGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVI MDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCP HRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKR MKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAG LRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVS LPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGT KGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRK PSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAA GEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVAL AKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
