Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0047
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821044
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5661nts
Open reading frame: 1 to 5661
ATGGCGGCGCGGGGCAGAGGGCTGCTGCTTCTGACACTGTCGGTGCTTTTAGCGGCTGGC
CCTTCTGCCGCTGCGGCCAAGCTCAACATCCCCAAAGTGCTCCTGCCCTTCACGCGGGCC
ACGCGCGTTAACTTCACGCTGGAGGCTTCGGAGGGCTGCTACCGCTGGTTGTCCACACGA
CCGGAAGTGGCCAGCATCGAGCCGTTGGGCCTGGATGAGCAGCAATGCTCCCAGAAGGCA
GTGGTGCAGGCCCGCCTGACCCAGCCTGCTCGCCTCACCAGCATCATCTTCGCAGAGGAC
ATCACCACAGGCCAGGTCCTCCGCTGTGATGCCATTGTGGACCTCATCCATGACATCCAG
ATCGTCTCCACCACCCGCGAGCTCTACCTGGAGGACTCCCCCCTGGAGCTGAAGATCCAG
GCCCTGGACTCTGAAGGGAACACCTTCAGCACTCTGGCTGGACTGGTCTTCGAGTGGACG
ATTGTGAAGGACTCCGAGGCGGACAGGTTCTCAGACTCCCACAATGCGCTGCGAATCCTC
ACTTTCTTGGAGTCTACGTACATCCCTCCTTCTTACATCTCAGAGATGGAGAAGGCTGCC
AAGCAAGGGGACACCATCCTGGTGTCTGGGATGAAGACCGGGAGCTCCAAGCTCAAGGCT
CGCATCCAGGAGGCTGTCTACAAGAATGTACGCCCTGCAGAAGTGAGGCTCCTGATTTTG
GAAAACATCCTTCTGAACCCGGCCTATGACGTCTACCTGATGGTGGGAACCTCCATTCAC
TACAAGGTGCAGAAGATCAGGCAAGGGAAAATTACAGAACTGTCCATGCCTTCCGATCAG
TACGAGTTGCAGCTTCAGAACAGCATCCCGGGACCCGAAGGGGACCCAGCCAGGCCGGTG
GCTGTCTTGGCCCAGGACACGTCGATGGTCACTGCACTGCAGCTGGGACAGAGCAGTCTC
GTCCTTGGCCACAGGAGTATTCGCATGCAAGGTGCTTCTAGGTTACCCAACAGCACTATC
TACGTGGTCGAACCTGGATACCTAGGGTTCACTGTTCACCCTGGTGACAGGTGGGTGCTG
GAAACCGGCCGCCTGTATGAAATCACCATCGAAGTTTTTGACAAGTTCAGCAACAAGGTC
TATGTATCTGACAACATCCGAATTGAAACTGTGCTTCCTGCTGAGTTCTTCGAGGTGCTC
TCGTCCTCCCAGAATGGGTCATACCATCGCATCAGGGCACTAAAGAGGGGACAGACGGCC
ATTGACGCGGCCCTGACCTCTGTGGTGGACCAGGATGGAGGGGTCCACATACTACAGGTG
CCTGTGTGGAACCAGCAGGAGGTGGAAATTCACATCCCGATCACCCTGTATCCCAGCATC
TTGACATTTCCGTGGCAACCAAAGACGGGCGCCTATCAGTACACAATAAGGGCCCACGGT
GGCAGTGGGAACTTCAGCTGGTCTTCGTCAAGCCACCTGGTTGCCACAGTTACTGTCAAG
GGCGTGATGACCACAGGCAGTGACATCGGGTTCAGTGTGATCCAGGCACATGATGTGCAG
AACCCACTCCATTTCGGTGAGATGAAGGTGTATGTGATCGAGCCCCATAGTATGGAGTTT
GCCCCGTGCCAGGTGGAGGCACGTGTGGGACAGGCCCTGGAACTGCCCCTGAGGATCAGT
GGCCTCATGCCCGGCGGAGCCAGTGAAGTGGTCACCTTGAGCGACTGCTCCCACTTTGAC
TTGGCTGTCGAGGTTGAGAACCAGGGTGTGTTCCAGCCACTCCCAGGGAGGCTGCCGCCA
GGCTCTGAGCACTGCAGCGGCATCCGGGTAAAGGCCGAGGCCCAGGGATCTACCACGCTT
CTTGTGAGCTACAGACACGGCCACGTCCACCTGAGTGCCAAGATCACCATTGCTGCCTAC
CTGCCCCTCAAGGCTGTGGATCCCTCCTCTGTTGCCTTGGTAACCCTGGGCTCCTCAAAG
GAGATGCTGTTTGAAGGAGGTCCCAGACCTTGGATCCTCGAGCCGTCCAAATTCTTCCAG
AACGTCACCGCTGAGGACACTGACAGCATCGGCCTGGCTCTCTTTGCCCCCCATTCCTCC
CGGAATTATCAGCAACACTGGATCCTTGTGACCTGTCAGGCCTTGGGTGAGCAGGTCATC
GCCCTGTCGGTGGGGAACAAGCCCAGCCTCACCAACCCCTTTCCTGCGGTGGAGCCTGCC
GTGGTGAAGTTCGTCTGCGCCCCACCGTCTAGGCTCACCCTTGCGCCTGTCTACACCAGC
CCCCAGCTCGACATGTCCTGTCCGCTGCTTCAGCAGAACAAGCAAGTGGTCCCAGTGTCC
AGCCACCGCAACCCCCGGCTGGACCTGGCTGCTTACGACCAGGAGGGCCGCCGGTTCGAC
AACTTCAGCTCTCTGAGCATCCAGTGGGAGTCCACCAGGCCAGTGTTGGCCAGCATCGAG
CCTGAGCTGCCCATGCAGCTGGTGTCCCAGGACGATGAGAGTGGCCAAAAGAAGCTGCAC
GGTTTGCAGGCCATTTTGGTTCACGAGGCATCAGGAACCACAGCCATCACTGCCACTGCC
ACTGGCTACCAGGAGTCCCACCTCAGCTCTGCCAGAACAAAGCAGCCGCATGACCCTCTG
GTGCCTCTGTCGGCCTCCATAGAGCTCATCCTGGTGGAGGACGTGAGGGTGAGCCCAGAA
GAGGTGACCATCTACAACCACCCTGGCATCCAGGCAGAGCTCCGCATCAGGGAAGGCTCC
GGTTACTTCTTCCTCAACACCAGCACCGCAGATGTTGTCAAGGTGGCCTACCAGGAGGCC
AGGGGTGTCGCCATGGTGCACCCTTTGCTCCCGGGCTCATCCACCATCATGATCCATGAC
TTGTGCCTCGTCTTCCCGGCCCCAGCCAAGGCTGTCGTTTACGTGTCGGACATTCAGGAG
CTGTACATCCGTGTGGTTGACAAGGTGGAGATTGGGAAGACAGTGAAGGCATACGTCCGC
GTGCTGGACTTGCACAAGAAGCCCTTCCTTGCCAAATACTTCCCCTTTATGGACCTGAAG
CTCCGAGCAGCCTCCCCGATCATTACATTGGTGGCCCTTGATGAAGCCCTTGACAACTAC
ACCATCACATTCCTCATCCGCGGTGTGGCCATCGGCCAGACCAGTCTAACTGCAAGTGTG
ACCAATAAAGCTGGACAGAGAATCAACTCAGCCCCACAACAGATTGAAGTCTTTCCCCCG
TTCAGGCTGATGCCCAGGAAGGTCACACTGCTTATCGGGGCCACGATGCAGGTCACCTCC
GAGGGCGGCCCTCAGCCTCAGTCCAACATCCTTTTCTCCATCAGCAATGAGAGCGTTGCG
CTGGTGAGCGCTGCTGGGCTGGTACAGGGCCTCGCCATCGGGAACGGCACTGTGTCTGGG
CTCGTGCAGGCAGTGGATGCAGAGACAGGCAAGGTGGTCATCATCTCTCAGGACCTCGTG
CAGGTCGAGGTGCTGCTGCTAAGGGCCGTGAGGATCCGCGCCCCCATCATGCGGATGAGG
ACCGGCACCCAGATGCCCATCTATGTCACCGGCATCACCAACCACCAGAACCCTTTCTCC
TTTGGCAATGCCGTGCCAGGCCTGACCTTCCACTGGTCTGTCACCAAGCGGGACGTCCTG
GACCTCCGAGGGCGGCACCACGAGGCGTCGATTCGACTCCCGTCACAGTACAACTTTGCT
ATGAACGTGCTCGGCCGGGTAAAAGGCCGGACCGGGCTGAGGGTGGTGGTCAAGGCTGTG
GACCCCACATCGGGGCAGCTGTATGGCCTGGCCAGAGAACTCTCGGATGAGATCCAAGTC
CAGGTGTTTGAGAAGCTGCAGCTGCTCAACCCTGAAATAGAAGCAGAACAAATATTAATG
TCGCCCAACTCATATATAAAGCTGCAGACAAACAGGGATGGTGCAGCCTCTCTGAGCTAC
CGCGTCCTGGATGGACCCGAAAAGGTTCCAGTTGTGCATGTTGATGAGAAAGGCTTTCTA
GCATCAGGGTCCATGATCGGGACATCCACCATCGAAGTGATTGCACAAGAGCCCTTTGGG
GCCAACCAAACCATCATTGTTGCTGTAAAGGTATCCCCTGTTTCCTACCTGAGGGTTTCC
ATGAGCCCTGTCCTGCACACCCAGAACAAGGAGGCCCTGGTGGCCGTGCCTTTGGGAATG
ACCGTGACATTCACTGTCCACTTCCACGACAACTCTGGAGATGTCTTCCATGCTCACAGT
TCGGTCCTCAACTTTGCTACTAACAGAGACGACTTTGTGCAGATCGGGAAGGGCCCCACC
AACAACACATGCGTTGTCCGCACAGTCAGCGTGGGCCTGACACTGCTGCGTGTGTGGGAC
GCAGAGCACCCGGGACTCTCGGACTTCATGCCCCTCCCTGTCCTACAGGCCATCTCCCCA
GAGCTGTCTGGGGCCATGGTGGTGGGGGACGTGCTCTGTCTGGCCACTGTTCTGACCAGC
CTGGAAGGCCTGTCTGGAACCTGGAGCTCCTCGGCCAACAGCATCCTCCACATCGACCCC
AAGACGGGTGTGGCTGTGGCCCGGGCCGTGGGATCCGTGACGGTTTACTATGAGGTCGCT
GGGCACCTGAGGACCTACAAGGAGGTGGTGGTCAGCGTCCCTCAGAGGATCATGGCCCGT
CACCTCCACCCCATCCAGACCAGCTTCCAGGAGGCTACAGCCTCCAAAGTGATTGTTGCC
GTGGGAGACAGAAGCTCTAACCTGAGAGGCGAGTGCACCCCCACCCAGAGGGAAGTCATC
CAGGCCTTGCACCCAGAGACACTCATCAGCTGCCAGTCCCAGTTCAAGCCGGCCGTCTTT
GATTTCCCATCTCAAGATGTGTTCACCGTGGAGCCACAGTTTGACACTGCTCTCGGCCAG
TACTTCTGCTCAATCACAATGCACAGGCTGACGGACAAGCAGCGGAAGCACCTGAGCATG
AAGAAGACAGCTCTGGTGGTCAGTGCCTCCCTCTCCAGCAGCCACTTCTCCACAGAACAG
GTGGGGGCCGAGGTGCCCTTCAGCCCAGGTCTTTTCGCCGACCAGGCTGAAATCCTTTTG
AGCAACCACTACACCAGTTCCGAGATCAGGGTCTTTGGTGCCCCGGAGGTTCTGGAGAAC
TTGGAGGTGAAATCCGGGTCCCCGGCCGTGCTGGCATTCGCAAAGGAGAAGTCTTTTGGG
TGGCCCAGCTTCATCACATACACGGTCGGCGTCTTGGACCCCGCGGCTGGCAGCCAAGGG
CCTCTGTCCACTACCCTGACCTTCTCCAGCCCCGTGACCAACCAAGCCATTGCCATCCCA
GTGACAGTGGCTTTTGTGGTGGATCGCCGTGGGCCCGGTCCTTATGGAGCCAGCCTCTTC
CAGCACTTCCTGGATTCCTACCAGGTCATGTTCTTCACGCTCTTCGCCCTGTTGGCTGGG
ACAGCGGTCATGATCATAGCCTACCACACTGTCTGCACGCCCCGGGATCTTGCTGTGCCT
GCAGCCCTCACGCCTCGAGCCAGCCCTGGACACAGCCCCCACTATTTCGCTGCCTCATCA
CCCACATCTCCCAATGCATTGCCTCCTGCTCGCAAAGCCAGCCCTCCCTCAGGGCTGTGG
AGCCCAGCCTATGCCTCCCAC
Protein sequence predicted based on nucleotide sequence
MAARGRGLLLLTLSVLLAAGPSAAAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWLSTR PEVASIEPLGLDEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHDIQ IVSTTRELYLEDSPLELKIQALDSEGNTFSTLAGLVFEWTIVKDSEADRFSDSHNALRIL TFLESTYIPPSYISEMEKAAKQGDTILVSGMKTGSSKLKARIQEAVYKNVRPAEVRLLIL ENILLNPAYDVYLMVGTSIHYKVQKIRQGKITELSMPSDQYELQLQNSIPGPEGDPARPV AVLAQDTSMVTALQLGQSSLVLGHRSIRMQGASRLPNSTIYVVEPGYLGFTVHPGDRWVL ETGRLYEITIEVFDKFSNKVYVSDNIRIETVLPAEFFEVLSSSQNGSYHRIRALKRGQTA IDAALTSVVDQDGGVHILQVPVWNQQEVEIHIPITLYPSILTFPWQPKTGAYQYTIRAHG GSGNFSWSSSSHLVATVTVKGVMTTGSDIGFSVIQAHDVQNPLHFGEMKVYVIEPHSMEF APCQVEARVGQALELPLRISGLMPGGASEVVTLSDCSHFDLAVEVENQGVFQPLPGRLPP GSEHCSGIRVKAEAQGSTTLLVSYRHGHVHLSAKITIAAYLPLKAVDPSSVALVTLGSSK EMLFEGGPRPWILEPSKFFQNVTAEDTDSIGLALFAPHSSRNYQQHWILVTCQALGEQVI ALSVGNKPSLTNPFPAVEPAVVKFVCAPPSRLTLAPVYTSPQLDMSCPLLQQNKQVVPVS SHRNPRLDLAAYDQEGRRFDNFSSLSIQWESTRPVLASIEPELPMQLVSQDDESGQKKLH GLQAILVHEASGTTAITATATGYQESHLSSARTKQPHDPLVPLSASIELILVEDVRVSPE EVTIYNHPGIQAELRIREGSGYFFLNTSTADVVKVAYQEARGVAMVHPLLPGSSTIMIHD LCLVFPAPAKAVVYVSDIQELYIRVVDKVEIGKTVKAYVRVLDLHKKPFLAKYFPFMDLK LRAASPIITLVALDEALDNYTITFLIRGVAIGQTSLTASVTNKAGQRINSAPQQIEVFPP FRLMPRKVTLLIGATMQVTSEGGPQPQSNILFSISNESVALVSAAGLVQGLAIGNGTVSG LVQAVDAETGKVVIISQDLVQVEVLLLRAVRIRAPIMRMRTGTQMPIYVTGITNHQNPFS FGNAVPGLTFHWSVTKRDVLDLRGRHHEASIRLPSQYNFAMNVLGRVKGRTGLRVVVKAV DPTSGQLYGLARELSDEIQVQVFEKLQLLNPEIEAEQILMSPNSYIKLQTNRDGAASLSY RVLDGPEKVPVVHVDEKGFLASGSMIGTSTIEVIAQEPFGANQTIIVAVKVSPVSYLRVS MSPVLHTQNKEALVAVPLGMTVTFTVHFHDNSGDVFHAHSSVLNFATNRDDFVQIGKGPT NNTCVVRTVSVGLTLLRVWDAEHPGLSDFMPLPVLQAISPELSGAMVVGDVLCLATVLTS LEGLSGTWSSSANSILHIDPKTGVAVARAVGSVTVYYEVAGHLRTYKEVVVSVPQRIMAR HLHPIQTSFQEATASKVIVAVGDRSSNLRGECTPTQREVIQALHPETLISCQSQFKPAVF DFPSQDVFTVEPQFDTALGQYFCSITMHRLTDKQRKHLSMKKTALVVSASLSSSHFSTEQ VGAEVPFSPGLFADQAEILLSNHYTSSEIRVFGAPEVLENLEVKSGSPAVLAFAKEKSFG WPSFITYTVGVLDPAAGSQGPLSTTLTFSSPVTNQAIAIPVTVAFVVDRRGPGPYGASLF QHFLDSYQVMFFTLFALLAGTAVMIIAYHTVCTPRDLAVPAALTPRASPGHSPHYFAASS PTSPNALPPARKASPPSGLWSPAYASH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
