Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0058
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821045
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5823nts
Open reading frame: 1 to 5823
ATGAGTTCAGACTCAGAATTGGCTGTTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCT
GAAAGGGAGCGCATTGAGGCCCAGAATAGGCCCTTTGATGCCAAAACATCTGTCTTTGTG
GCGGAGCCCAAAGAATCCTTTGTCAAAGGGACCATCCAGAGCAGAGAAGGAGGAAAAGTG
ACGGTGAAGACTGAGGGAGGAGCGACTCTGACAGTGAAGGATGATCAGGTCTTCCCCATG
AACCCTCCCAAATATGACAAGATCGAGGATATGGCCATGATGACTCATCTGCATGAGCCT
GCTGTGCTGTACAACCTCAAAGAACGTTATGCAGCCTGGATGATCTACACCTATTCAGGT
CTATTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCTGTGTATAAGCCCGAGGTGGTG
ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGAC
AACGCCTATCAGTTCATGCTGACTGACCGAGAGAATCAGTCAATCCTGATCACTGGAGAA
TCTGGTGCAGGGAAGACTGTGAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCA
GTTACTGGTGAGAAGAAGAAGGAAGAAATTACTTCTGGCAAAATACAGGGGACTCTGGAA
GATCAAATCATCAGTGCCAACCCCCTACTGGAGGCCTTTGGCAACGCCAAGACCGTGAGG
AATGACAACTCCTCTCGCTTTGGTAAATTCATCAGAATCCACTTTGGCACTACTGGAAAA
CTGGCATCTGCTGATATTGAAACATATCTGCTAGAGAAGTCTAGAGTTGTTTTCCAGCTT
AAGGCTGAGAGAAGTTATCATATTTTTTACCAGATTACATCGAATAAGAAACCAGAACTT
ATTGAAATGCTTCTGATTACCACGAACCCATATGATTACCCATTTGTCAGTCAAGGGGAG
ATCAGTGTGGCCAGCATCGATGATCAGGAAGAACTGATGGCCACAGATAGTGCTATTGAT
ATTTTGGGCTTTACTAATGAAGAAAAGGTGTCCATTTACAAGCTCACGGGGGCTGTGATG
CATTATGGGAACCTAAAATTTAAGCAAAAGCAGCGTGAGGAGCAAGCAGAGCCAGATGGC
ACAGAAGTTGCTGACAAGGCGGCCTACCTCCAGAGTCTGAACTCTGCAGATCTGCTCAAA
GCTCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTA
GAACAGGTGTCCAACGCAGTAGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTG
TGGATGGTTGCCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATC
GGGGTCTTGGACATTGCTGGTTTTGAGATTTTTGATTTCAACAGCCTGGAGCAGCTGTGC
ATCAACTTCACCAATGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAG
CAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCATCGACTTCGGGATGGACCTG
GCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTGGAAGAGGAG
TGCATGTTCCCTAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG
GGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAGGCCGAGGCCCACTTC
GCTCTGATTCACTATGCTGGTGTTGTGGACTACAACATTACTGGCTGGCTGGAGAAGAAC
AAGGACCCCCTGAATGAGACAGTGGTTGGACTGTACCAGAAGTCTGCAATGAAAACTCTA
GCTCAGCTCTTCTCTGGGGCTCAAACTGCTGAAGGAGAGGGAGCTGGTGGAGGGGCCAAG
AAAGGTGGTAAGAAGAAGGGCTCTTCTTTCCAGACAGTGTCTGCCCTTTTCAGAGAGAAT
TTGAACAAGCTGATGACCAACCTCAGGAGTACCCATCCTCACTTTGTGAGGTGTATCATC
CCCAATGAGACAAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTCCACCAGCTGAGG
TGTAACGGTGTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTTCCAAGCAGAATCCTT
TATGCAGACTTCAAACAGAGATACAAGGTATTAAATGCAAGTGCAATCCCTGAAGGGCAA
TTCATTGATAGCAAGAAGGCCTCTGAGAAGCTCCTTGCATCCATCGACATTGACCACACC
CAGTATAAATTTGGGCACACCAAGGTCTTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAG
GAGATGCGAGATGACAAGCTGGCCCAGCTGATTACCCGAACCCAGGCCAGGTGCAGAGGG
TTCTTGGCAAGAGTGGAGTACCAGAGGATGGTGGAGAGAAGGGAGGCCATCTTCTGTATC
CAGTACAATATCAGATCCTTCATGAATGTCAAGCACTGGCCCTGGATGAAACTCTTCTTC
AAGATCAAGCCTCTGTTGAAGAGTGCAGAAACTGAGAAGGAGATGGCCACCATGAAGGAA
GAATTTCAGAAAATTAAAGACGAACTTGCCAAGTCAGAGGCAAAAAGGAAGGAACTGGAA
GAAAAGATGGTGACGCTGTTGAAAGAAAAAAATGACTTGCAGCTCCAAGTTCAGGCTGAA
GCCGAAGGCTTGGCTGATGCAGAGGAAAGGTGTGACCAGCTAATCAAAACCAAAATCCAG
CTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGGATGAGGAAGAGATCAATGCT
GAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACATT
GATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAG
GTGAAAAACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAG
GAGAAGAAGGCTCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAG
GACAAAGTCAACACCCTGACCAAAGCTAAAATCAAACTTGAACAACAAGTGGATGATCTT
GAAGGGTCCTTGGAGCAAGAAAAGAAACTTCGCATGGACCTAGAAAGGGCTAAGAGGAAA
CTTGAGGGTGACTTGAAGTTGGCCCAAGAATCCATAATGGACATTGAAAATGAGAAACAG
CAACTTGATGAAAAGCTCAAAAAGAAAGAGTTTGAAATCAGCAATCTGCAAAGCAAGATT
GAAGATGAACAGGCACTTGGCATTCAATTGCAGAAGAAAATTAAAGAATTGCAAGCCCGC
ATTGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAG
CAGCGCTCTGACCTCTCCCGGGAGCTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGT
GGGGCCACTTCAGCCCAGATTGAGATGAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATG
CGCAGGGACCTGGAGGAGGCCACCCTACAGCATGAAGCCACAGCGGCCACCCTGAGGAAG
AAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGAGTGAAG
CAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATTGATGACCTTGCTAGTAAT
GTAGAAACGGTGTCCAAAGCCAAGGGAAACCTAGAGAAAATGTGCCGGACTCTAGAGGAC
CAACTGAGTGAACTGAAATCAAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTGACT
GCGCAGAGGGGGCGCCTGCAGACTGAATCTGGTGAGTTTTCACGCCAGCTTGATGAAAAG
GAAGCTCTGGTGTCTCAGTTATCAAGAGGCAAACAAGCCTTTACTCAACAGATTGAAGAA
TTAAAGAGGCAACTTGAAGAGGAGATAAAAGCCAAGAACGCCCTGGCGCATGCCCTGCAG
TCTTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAATCCAAG
GCCGAGCTGCAGAGAGCACTGTCCAAGGCCAACACCGAGGTTGCCCAATGGAGGACCAAA
TACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCC
CAGCGGCTGCAGGCAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTC
GAAAAGACGAAGCAGCGGCTGCAGAATGAGGTCGAGGACCTCATGCTTGATGTGGAGAGG
ACAAATGCCGCCTGTGCCGCCCTTGACAAAAAGCAAAGGAACTTCGATAAGATCCTGGCA
GAATGGAAACAGAAATGTGAGGAAACGCATGCTGAGCTTGAGGCCTCCCAGAAGGAGGCC
CGTTCCCTTGGCACTGAGCTGTTCAAGATAAAGAATGCCTATGAGGAATCTTTGGATCAG
CTAGAAACCCTGAAGCGAGAGAACAAAAACTTACAGCAGGAGATTTCTGACCTCACGGAA
CAGATTGCAGAAGGAGGGAAACGTATCCATGAACTGGAGAAAATAAAGAAACAAGTGGAA
CAAGAAAAGTGTGAACTTCAGGCTGCTTTAGAAGAAGCAGAGGCATCTCTTGAACATGAA
GAGGGAAAGATCCTGCGCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGG
AAAATTGCTGAAAAAGATGAGGAAATTGACCAGCTGAAGAGAAACCACATTAGAATCGTG
GAGTCCATGCAGAGCACGCTGGATGCTGAGATCAGGAGTAGGAATGATGCCATTAGGCTC
AAGAAGAAGATGGAGGGTGACCTCAATGAAATGGAAATCCAGCTGAACCATGCCAACCGC
ATGGCTGCTGAGGCCCTGAGGAACTACAGGAACACCCAAGGCATCCTCAAGGATACCCAG
ATCCACCTGGATGATGCTCTCCGGAGCCAGGAGGACCTGAAGGAACAGCTGGCCATGGTG
GAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAACAG
ACAGAGAGGAGCAGAAAAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTTCAG
CTACTGCACACCCAGAACACCAGCCTGATCAACACCAAGAAGAAGCTGGAGACAGATATT
TCCCAAATGCAAGGAGAGATGGAGGACATTCTCCAGGAAGCCCGCAATGCAGAAGAAAAG
GCCAAGAAGGCCATCACTGATGCCGCCATGATGGCTGAGGAGCTGAAGAAGGAGCAGGAC
ACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGATCTGCAG
CTCCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAGAAA
CTGGAGGCCAGGGTACGGGAGCTGGAAGGAGAGGTTGAGAGTGAGCAAAAGCGTAATGCT
GAGGCTGTCAAAGGTCTGCGCAAACATGAGAGGCGAGTGAAGGAACTCACTTACCAGACG
GAAGAAGATAGAAAGAATATTCTCAGGCTTCAAGATTTGGTAGATAAACTTCAGGCAAAA
GTGAAATCTTATAAGAGACAAGCTGAGGAGGCTGAGGAACAATCCAACACCAATCTAGCT
AAATTCCGCAAGCTCCAGCATGAGCTGGAGGAGGCCGAGGAACGGGCTGACATTGCTGAG
TCCCAGGTGAACAAACTGCGGGTGAAGAGCCGGGAGGTTCACACAAAAGTCATAAGTGAA
GAG
Protein sequence predicted based on nucleotide sequence
MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKV TVKTEGGATLTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSG LFCVTVNPYKWLPVYKPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGE SGAGKTVNTKRVIQYFATIAVTGEKKKEEITSGKIQGTLEDQIISANPLLEAFGNAKTVR NDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQLKAERSYHIFYQITSNKKPEL IEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKVSIYKLTGAVM HYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTV EQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLC INFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEE CMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKN KDPLNETVVGLYQKSAMKTLAQLFSGAQTAEGEGAGGGAKKGGKKKGSSFQTVSALFREN LNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRIL YADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLE EMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFF KIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAE AEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDI DDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEE DKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQ QLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEK QRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRK KHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLED QLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEE LKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTK YETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVER TNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKEARSLGTELFKIKNAYEESLDQ LETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKCELQAALEEAEASLEHE EGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRL KKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMV ERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDI SQMQGEMEDILQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQ LRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQT EEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLAKFRKLQHELEEAEERADIAE SQVNKLRVKSREVHTKVISEE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
