Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0045
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821047
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5580nts
Open reading frame: 1 to 5580
ATGGGCTGGGGAAGCCGCTGCTGCTGTCCTGGACGTTTGGACCTGCTTTGCGTTTTAGCG
CTGCTCGGAGGATGCCTTCTTCCCGTGTGTCGGACACGCGTCTACACCAACCACTGGGCA
GTCAAAATCGCCGGGGGCTTCCCGGAGGCTAACCGTATCGCCAGCAAGTACGGATTCATC
AACATAGGACAGATAGGGGCCCTGAAGGACTACTACCACTTCTACCATAGCAGGACGATT
AAAAGGTCCGTTATCTCGAGCAGAGGGACCCACAGTTTCATTTCAATGGAACCAAAGGTG
GAATGGATCCAACAGCAAGTGGTAAAAAAGCGGACAAAGAGGGATTATGACTTCAGTCGT
GCCCAGTCTACCTATTTCAATGATCCCAAGTGGCCCAGCATGTGGTATATGCACTGCAGT
GACAATACACATCCCTGCCAGTCTGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTAC
ACGGGAAAGAACATTGTGGTCACTATCCTGGATGACGGAATTGAGAGAACCCATCCAGAT
CTGATGCAAAACTACGATGCCCTGGCAAGTTGCGACGTGAATGGGAATGACTTGGACCCA
ATGCCTCGTTATGATGCAAGCAACGAGAACAAGCATGGGACTCGCTGTGCTGGAGAAGTG
GCAGCCGCTGCAAACAATTCGCACTGCACAGTCGGAATTGCTTTCAACGCCAAGATCGGA
GGAGTGCGAATGCTGGACGGAGATGTCACGGACATGGTTGAAGCAAAATCAGTTAGCTTC
AACCCCCAGCACGTGCACATTTACAGCGCCAGCTGGGGACCGGATGATGATGGCAAGACT
GTGGACGGACCAGCCCCCCTCACCCGGCAAGCCTTTGAAAACGGCGTTAGAATGGGACGG
AGAGGCCTCGGCTCTGTGTTTGTTTGGGCATCTGGAAATGGTGGAAGGAGCAAAGACCAC
TGCTCCTGTGATGGCTACACCAACAGCATCTACACCATCTCCATCAGCAGCACTGCAGAA
AGCGGCAAGAAACCTTGGTACCTGGAAGAGTGTTCATCCACGCTGGCCACAACCTACAGC
AGCGGGGAGTCCTACGATAAGAAAATCATCACTACAGATCTGAGGCAGCGTTGCACGGAC
AACCACACTGGGACGTCAGCCTCAGCCCCCATGGCTGCAGGCATCATTGCGCTGGCCCTG
GAAGCCAATCCGTTTCTGACCTGGAGAGACGTACAGCATGTTATTGTCAGGACTTCCCGT
GCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCAT
CTTTATGGATTTGGACTGATGGACGCAGAAGCCATGGTGATGGAGGCAGAGAAGTGGACC
ACCGTTCCCCGGCAGCACGTGTGTGTCGAGAGCACAGACCGACAAATCAAGACAATCCGC
CCTAACAGTGCAGTGCGCTCCATCTACAAAGCTTCAGGCTGCTCGGATAACCCCAACCGC
CATGTCAACTACCTGGAGCACGTCGTTGTGCGCATCACCATCACCCACCCCAGGAGAGGA
GACTTGGCCATCTACCTGACCTCGCCCTCTGGAACTAGGTCCCAGCTTTTGGCCAACAGG
CTATTTGATCACTCCATGGAAGGATTCAAAAACTGGGAGTTCATGACCATTCATTGCTGG
GGAGAAAGAGCTGCTGGTGACTGGGTCCTTGAAGTTTATGATACTCCCTCTCAGCTAAGG
AACTTTAAGACTCCAGGTAAACTGAAAGAATGGTCTTTGGTCCTCTACGGCACCTCCGTG
CAGCCATATTCACCAACCAATGAATTTCCGAAAGTGGAACGGTTCCGCTATAGCCGAGTT
GAAGACCCCACAGACGACTATGGCACAGAGGATTATGCAGGTCCCTGCGACCCTGAGTGC
AGTGAGGTTGGCTGTGACGGGCCAGGACCAGACCACTGCAATGACTGTTTGCACTACTAC
TACAAGCTGAAAAACAATACCAGGATCTGTGTCTCCAGCTGCCCCCCTGGCCACTACCAC
GCCGACAAGAAGCGCTGCAGGAAGTGTGCCCCCAACTGTGAGTCTTGCTTTGGGAGCCAT
GGTGACCAATGCATGTCCTGCAAATATGGATACTTTCTGAATGAAGAAACCAACAGCTGT
GTTACTCACTGCCCTGATGGGTCATATCAGGATACCAAGAAAAATCTTTGCCGGAAATGC
AGTGAAAACTGCAAGACATGTACTGAATTCCATAACTGTACAGAATGTAGGGATGGGTTA
AGCCTGCAGGGATCACGGTGCTCTGTCTCCTGTGAAGATGGACGGTATTTCAACGGCCAG
GACTGCCAGCCCTGCCACCGCTTCTGCGCCACTTGTGCTGGGGCCGGAGCTGATGGGTGC
ATTAACTGCACAGAGGGCTACTTCATGGAGGATGGGAGATGCGTGCAGAGCTGTAGTATC
AGCTATTACTTTGACCACTCTTCAGAGAATGGATACAAATCCTGCAAAAAATGTGATATC
AGTTGTTTGACGTGCAATGGCCCAGGATTCAAGAACTGTACAAGCTGCCCTAGTGGGTAT
CTCTTAGACTTAGGAATGTGTCAAATGGGAGCCATTTGCAAGGATGGAGAATATGTTGAT
GAGCATGGCCACTGCCAGACCTGTGAGGCCTCATGTGCCAAGTGCCAGGGACCAACCCAG
GAAGACTGCACTACCTGCCCCATGACAAGGATTTTTGATGATGGCCGCTGTGTTTCGAAC
TGCCCCTCATGGAAATTTGAATTTGAGAACCAATGCCATCCATGCCACCATACCTGCCAG
AGATGCCAAGGAAGTGGCCCTACCCACTGCACCTCCTGTGGAGCAGACAACTATGGCCGA
GAGCACTTCCTGTACCAGGGAGAGTGTGGAGATAGCTGCCCAGAGGGCCACTATGCCACT
GAGGGGAACACATGCCTGCCCTGCCCAGACAACTGTGAGCTTTGCCACAGCGTGCATGTC
TGCACAAGATGCATGAAGGGCTACTTCATAGCGCCCACCAACCACACATGCCAGAAGTTA
GAGTGTGGACAAGGTGAAGTCCAAGACCCAGACTATGAAGAATGTGTCCCTTGTGAAGAA
GGATGTCTGGGATGCAGCTTGGATGATCCAGGAACATGTACATCTTGCGCTATGGGGTAT
TACAGGTTTGATCACCATTGTTATAAAACCTGTCCTGAGAAGACCTACAGTGAGGAAGTG
GAATGCAAGGCGTGTGATAGTAACTGTGGCAGCTGTGACCAGAATGGGTGTTACTGGTGT
GAAGAGGGCTTCTTTCTCTTAGGTGGCAGTTGTGTGAGGAAATGTGGTCCTGGATTCTAT
GGTGACCAAGAAATGGGAGAATGTGAGTCCTGCCACCGAGCATGCGAAACCTGCACAGGC
CCTGGTCATGACGAGTGCAGTAGCTGCCAGGAAGGACTGCAGCTGCTGAGAGGGATGTGC
GTGCATGCCACCAAGACCCAGGAGGAGGGCAAATTCTGGAATGATATTTTGAGAAAACTC
CAGCCTTGTCATTCTTCTTGTAAAACCTGCAATGGATCTGCAACTCTGTGCACTTCATGT
CCCAAAGGTGCATATCTTCTGGCTCAGGCCTGTGTTTCCTCCTGTCCCCAAGGCACATGG
CCTTCCGTAAGGAGTGGGAGCTGCGAGAACTGTACGGAGGCCTGTGCCATCTGCTCTGGA
GCCGATCTTTGCAAAAAATGCCAGATGCAGCCGGGCCACCCTCTCTTCCTCCATGAAGGA
AGGTGCTACTCCAAGTGCCCGGAGGGCTCTTATGCAGAAGACGGCATATGTGAACGCTGT
AGCTCTCCTTGCAGAACATGTGAAGGAAACGCCACCAACTGCCATTCTTGTGAAGGAGGC
CACGTCCTGCACCACGGAGTGTGCCAGGAAAACTGCCCCGAGAGGCACGTGGCTGTGAAG
GGGGTATGCAAGCATTGCCCAGAGATGTGTCAGGACTGCATCCATGAGAAAACATGCAAA
GAGTGCACGCCTGAGTTCTTCCTGCACGATGATATGTGCCACCAGTCCTGTCCCCGTGGC
TTCTATGCAGACTCGCGCCACTGTGTCCCCTGCCATAAAGACTGTCTGGAGTGCAGTGGC
CCCAAAGCCGACGACTGCGAGCTCTGTCTTGAGAGTTCCTGGGTCCTCTATGATGGACTG
TGCTTGGAGGAGTGTCCAGCAGGAACCTATTATGAAAAGGAGACTAAGGAGTGCAGAGAT
TGCCACAAGTCCTGCTTGACCTGCTCATCATCTGGGACCTGCACCACCTGTCAGAAAGGC
CTGATCATGAACCCTCGTGGGAGCTGCATGGCCAACGAGAAGTGCTCACCCTCCGAGTAC
TGGGATGAGGATGCTCCCGGGTGCAAGCCCTGCCATGTTAAGTGCTTCCACTGCATGGGG
CCGGCGGAGGACCAGTGTCAAACATGCCCCATGAACAGCCTTCTTCTCAACACAACCTGT
GTGAAGGACTGCCCAGAGGGCTATTATGCCGATGAGGACAGCAACAGGTGTGCCCACTGC
CACAGCTCTTGCAGGACATGTGAAGGGAGACACAGCAGGCAGTGCCACTCCTGCCGACCG
GGCTGGTTCCAGCTAGGAAAAGAGTGCCTGCTCCAGTGCAGGGAAGGATATTACGCAGAC
AACTCCACTGGCCGGTGTGAGAGGTGCAACAGGAGCTGCAAGGGATGCCAGGGCCCACGG
CCCACAGACTGCCTGTCTTGCGATAGATTTTTCTTTCTGCTCCGCTCCAAAGGAGAGTGT
CATCGCTCCTGCCCAGACCATTACTATGTAGAGCAAAGCACACAGACCTGTGAGAGATGC
CATCCGACTTGTGATCAATGCAAAGGAAAAGGAGCGTTGAATTGTTTATCCTGTGTGTGG
AGTTACCACCTCATGGGAGGGATCTGCACCTCGGACTGTCTTGTGGGGGAATACAGAGTG
GGAGAGGGAGAGAAGTTTAACTGTGAAAAATGCCACGAGAGCTGCATGGAATGCAAGGGA
CCAGGGGCCAAGAACTGCACCTTGTGCCCTGCCAACCTGGTGCTGCACATGGACGACAGC
CACTGCCTCCACTGCTGCAACACCTCTGATCCTCCCAGTGCCCAGGAGTGCTGTGACTGC
CAGGACACCACGGACGAATGCATCCTTCGAACAAGCAAGGTTAGGCCTGCAACTGAGCAT
TTCAAGACAGCTCTGTTCATCACCTCCTCCATGATGCTCGTGCTTCTGCTCGGGGCAGCT
GTGGTAGTGTGGAAGAAATCTCGTGGCCGAGTCCAGCCAGCAGCAAAGGCCGGCTATGAA
AAACTGGCCGACCCCAACAAGTCTTACTCCTCCTATAAGAGCAGCTATAGAGAGAGCACC
AGCTTTGAAGAGGATCAGGTGATTGAGTACAGGGATCGGGACTATGATGAGGATGATGAT
GATGACATCGTCTACATGGGCCAGGATGGCACAGTCTACCGGAAATTTAAATATGGGCTG
CTGGATGACGATGACATAGATGAGCTGGAATATGATGACGAGAGTTACTCCTACTACCAG
Protein sequence predicted based on nucleotide sequence
MGWGSRCCCPGRLDLLCVLALLGGCLLPVCRTRVYTNHWAVKIAGGFPEANRIASKYGFI NIGQIGALKDYYHFYHSRTIKRSVISSRGTHSFISMEPKVEWIQQQVVKKRTKRDYDFSR AQSTYFNDPKWPSMWYMHCSDNTHPCQSDMNIEGAWKRGYTGKNIVVTILDDGIERTHPD LMQNYDALASCDVNGNDLDPMPRYDASNENKHGTRCAGEVAAAANNSHCTVGIAFNAKIG GVRMLDGDVTDMVEAKSVSFNPQHVHIYSASWGPDDDGKTVDGPAPLTRQAFENGVRMGR RGLGSVFVWASGNGGRSKDHCSCDGYTNSIYTISISSTAESGKKPWYLEECSSTLATTYS SGESYDKKIITTDLRQRCTDNHTGTSASAPMAAGIIALALEANPFLTWRDVQHVIVRTSR AGHLNANDWKTNAAGFKVSHLYGFGLMDAEAMVMEAEKWTTVPRQHVCVESTDRQIKTIR PNSAVRSIYKASGCSDNPNRHVNYLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANR LFDHSMEGFKNWEFMTIHCWGERAAGDWVLEVYDTPSQLRNFKTPGKLKEWSLVLYGTSV QPYSPTNEFPKVERFRYSRVEDPTDDYGTEDYAGPCDPECSEVGCDGPGPDHCNDCLHYY YKLKNNTRICVSSCPPGHYHADKKRCRKCAPNCESCFGSHGDQCMSCKYGYFLNEETNSC VTHCPDGSYQDTKKNLCRKCSENCKTCTEFHNCTECRDGLSLQGSRCSVSCEDGRYFNGQ DCQPCHRFCATCAGAGADGCINCTEGYFMEDGRCVQSCSISYYFDHSSENGYKSCKKCDI SCLTCNGPGFKNCTSCPSGYLLDLGMCQMGAICKDGEYVDEHGHCQTCEASCAKCQGPTQ EDCTTCPMTRIFDDGRCVSNCPSWKFEFENQCHPCHHTCQRCQGSGPTHCTSCGADNYGR EHFLYQGECGDSCPEGHYATEGNTCLPCPDNCELCHSVHVCTRCMKGYFIAPTNHTCQKL ECGQGEVQDPDYEECVPCEEGCLGCSLDDPGTCTSCAMGYYRFDHHCYKTCPEKTYSEEV ECKACDSNCGSCDQNGCYWCEEGFFLLGGSCVRKCGPGFYGDQEMGECESCHRACETCTG PGHDECSSCQEGLQLLRGMCVHATKTQEEGKFWNDILRKLQPCHSSCKTCNGSATLCTSC PKGAYLLAQACVSSCPQGTWPSVRSGSCENCTEACAICSGADLCKKCQMQPGHPLFLHEG RCYSKCPEGSYAEDGICERCSSPCRTCEGNATNCHSCEGGHVLHHGVCQENCPERHVAVK GVCKHCPEMCQDCIHEKTCKECTPEFFLHDDMCHQSCPRGFYADSRHCVPCHKDCLECSG PKADDCELCLESSWVLYDGLCLEECPAGTYYEKETKECRDCHKSCLTCSSSGTCTTCQKG LIMNPRGSCMANEKCSPSEYWDEDAPGCKPCHVKCFHCMGPAEDQCQTCPMNSLLLNTTC VKDCPEGYYADEDSNRCAHCHSSCRTCEGRHSRQCHSCRPGWFQLGKECLLQCREGYYAD NSTGRCERCNRSCKGCQGPRPTDCLSCDRFFFLLRSKGECHRSCPDHYYVEQSTQTCERC HPTCDQCKGKGALNCLSCVWSYHLMGGICTSDCLVGEYRVGEGEKFNCEKCHESCMECKG PGAKNCTLCPANLVLHMDDSHCLHCCNTSDPPSAQECCDCQDTTDECILRTSKVRPATEH FKTALFITSSMMLVLLLGAAVVVWKKSRGRVQPAAKAGYEKLADPNKSYSSYKSSYREST SFEEDQVIEYRDRDYDEDDDDDIVYMGQDGTVYRKFKYGLLDDDDIDELEYDDESYSYYQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
