Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0006
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821048
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4845nts
Open reading frame: 1 to 4845
ATGGAGGCAGCTCCGCCCGGGCCTCCGTGGCCTCTGTTATTACTGCTTCTTCTTCTTCTG
GCGTTATGCGGCTGTCCTGCCCCTGCTGCGGCTTCTCCGCTTCTGCTATTTGCCAACCGA
CGGGACGTAAGGCTTGTGGACGCCGGCGGAGTCAAGCTGGAATCCACCATCGTTGTCAGC
GGCCTGGAGGATGCTGCCGCAGTGGACTTCCAGTTTTCCAAGGGAGCCGTGTACTGGACA
GACGTGAGCGAGGAGGCCATCAAGCAGACCTACCTGAACCAGACAGGGGCTGCCGTGCAG
AACGTGGTCATCTCCGGCCTGGTGTCTCCTGACGGCCTTGCCTGCGACTGGGTTGGCAAG
AAGCTGTACTGGACGGACTCAGAGACAAACCGCATCGAGGTGGCCAACCTCAATGGCACA
TCCCGGAAGGTGCTCTTCTGGCAGGACCTTGACCAGCCGAGAGCCATCGCCTTAGACCCC
GCTCACGGGTACATGTACTGGACAGACTGGGGTGAGACACCCCGGATTGAGCGGGCAGGG
ATGGATGGCAGCACCCGGAAGATCATTGTGGACTCGGACATTTACTGGCCCAATGGACTG
ACCATCGACCTGGAGGAGCAGAAGCTCTACTGGGCTGACGCCAAGCTCAGCTTCATCCAC
CGTGCCAACCTGGACGGCTCGTTCCGGCAGAAGGTGGTGGAGGGCAGTCTGACGCACCCC
TTTGCCCTTACGCTCTCTGGGGACACTCTGTACTGGACAGACTGGCAGACCCGCTCCATC
CATGCCTGCAACAAGCGCACTGGGGGCAAGAGGAAGGAGATCCTGAGTGCCCTCTACTCA
CCCATGGACATCCAGGTGCTGAGCCAGGAGCGGCAGCCTTTCTTCCACACTCGCTGTGAG
GAAGACAATGGCGGCTGCTCCCACCTGTGCCTGCTGTCCCCAAGCGAGCCTTTCTACACA
TGCGCTTGCCCCACGGGTGTGCAGCTGCAGGACAACGGAAGGACGTGTAAAGCAGGAGCT
GAGGAGGTGCTGCTTCTTGCCCGGCGGACAGACCTACGGAGGATCTCGCTTGACACGCCG
GACTTCACCGACATCGTGCTTCAGGTGGACGACATCCGGCACGCCATTGCCATCGACTAC
GACCCGCTAGAGGGCTATGTCTACTGGACAGATGACGAGGTGCGGGCTATCCGCAGAGCG
TATCTGGATGGGTCTGGGGCGCAGACACTGGTCAACACCGAGATCAACGACCCCGATGGC
ATCGCGGTTGACTGGGTGGCCAGAAACCTCTACTGGACCGACACGGGCACGGACCGCATT
GAGGTTACGCGCCTTAACGGCACCTCTCGAAAGATCCTTGTGTCGGAGGACCTGGACGAG
CCCCGAGCCATCGCACTGCACCCCGTGATGGGCCTCATGTACTGGACAGACTGGGGAGAG
AACCCTAAAATCGAGTGTGCCAACTTGGATGGACAGGAACGGCGTGTGTTAGTTAATGCC
TCCCTCGGGTGGCCCAACGGCCTGGCCCTGGACCTGCAGGAGGGGAAGCTCTATTGGGGA
GACGCCAAGACAGACAAGATCGAGGTGATCAATGTTGATGGGACGAAGAGGCGGACCCTC
CTGGAGGACAAGCTCCCGCACATTTTTGGGTTCACGCTGCTGGGGGACTTCATCTACTGG
ACTGACTGGCAGCGCCGCAGCATCGAGCGGGTGCACAAGGTCAAGGCCAGCCGGGACGTC
ATCATTGACCAGCTGCCTGACCTGATGGGGCTCAAAGCTGTGAATGTGGCCAAGGTCGTC
GGAACCAACCCGTGTGCGGACAGGAACGGCGGGTGCAGTCACCTGTGCTTCTTCACACCT
CACGCAACACGGTGTGGCTGCCCCATCGGCCTTGAGCTGCTGAGTGACATGAAGACCTGC
ATCGTGCCTGAGGCCTTCTTGGTCTTCACCAGCAGAGCCGCCATCCACAGGATCTCCCTC
GAAACCAATAACAACGACGTGGCCATCCCGCTCACGGGCGTCAAGGAGGCCTCAGCCCTG
GACTTTGATGTGTCCAACAACCACATCTACTGGACAGACGTCAGCCTGAAGACCATCAGC
CGCGCCTTCATGAACGGGAGCTCGGTGGAGCACGTGGTGGAGTTTGGCCTTGACTACCCC
GAGGGCATGGCCGTTGACTGGATGGGCAAGAACCTCTACTGGGCTGACACTGGGACCAAC
AGAATCGAAGTGGCGCGGCTGGACGGGCAGTTCCGGCAAGTCCTCGTGTGGAGGGACTTG
GACAACCCGAGATCGCTGGCTCTGGATCCCACCAAGGGCTACATCTACTGGACAGAGTGG
GGAGGAAAGCCGAGGATCGTGCGGGCCTTCATGGACGGGACCAACTGCATGACGCTTGTG
GACAAGGTGGGCCGGGCCAACGACCTCACCATTGACTACGCTGACCAGCGCCTCTACTGG
ACCGACCTGGACACCAACATGATCGAGTCGTCCAACATGCTGGGTCAGGAGCGGGTCGTG
ATTGCCGACGATCTCCCGCACCCGTTCGGTCTGACGCAGTACAGCGATTATATCTACTGG
ACAGACTGGAATCTGCACAGCATTGAGCGGGCCGACAAGACTAGCGGCAGGAACCGCACC
CTCATCCAGGGCCACCTGGACTTCGTGATGGACATCCTGGTGTTCCACTCCTCCCGCCAG
GATGGACTCAATGACTGTATGCACAACAACGGGCAGTGTGGGCAACTGTGTCTTGCTATC
CCCGGCGGACACCGCTGTGGATGCGCCTCACACTACACACTGGACCCCAGCAGTCGCAAC
TGTAGCCCGCCCACCACCTTCTTGCTGTTCAGCCAGAAATCTGCCATCAGTCGGATGATC
CCGGACGACCAGCACAGCCCGGATCTCATCCTGCCCCTGCATGGACTGAGGAACGTCAAA
GCCATCGACTATGACCCACTCGACAAGTTCATCTACTGGGTGGATGGGCGCCAGAACATC
AAGCGAGCCAAGGACGACGGGACCCAGCCCTTTGTTTTGACCTCTCTGAGCCAAGGCCAA
AACCCAGACAGGCAGCCCCACGACCTCAGCATCGACATCTACAGCCGGACACTGTTCTGG
ACGTGCGAGGCCACAAATACCATCAACGTCCATAGGCTGAGCGGGGAAGCCATGGGGGTG
GTGCTGCGTGGGGACCGCGACAAGCCTAGGGCCATTGTCGTCAATGCGGAGCGAGGGTAC
CTTTACTTCACCAACATGCAGGACCGGGCAGCCAAGATCGAACGAGCAGCCCTGGATGGC
ACCGAGCGCGAAGTCCTTTTCACAACCGGCCTTATCCGCCCTGTTGCCCTGGTGGTGGAC
AACACACTGGGCAAGCTGTTCTGGGTGGACGCGGACCTGAAGCGCATTGAGAGCTGTGAT
CTGTCAGGGGCTAACCGCCTGACCCTGGAGGACGCCAACATCGTGCAGCCTCTGGGCCTG
ACCATCCTTGGCAAGCATCTCTACTGGATCGACCGCCAGCAGCAGATGATCGAGCGTGTG
GAGAAGACCACCGGGGACAAGCGGACTCGCATCCAGGGCAGAGTCGCCCACCTCACTGGC
ATCCATGCAGTGGAGGAAGTCAGTCTGGAGGAGTTCTCAGCCCACCCATGTGCCCGTGAC
AATGGTGGCTGCTCCCACATCTGTATTGCTAAGGGTGATGGGACACCTCGGTGCTCATGC
CCAGTCCACCTCGTGCTCCTCCAGAACCTTCTGACCTGTGGAGAGCCGCCTACCTGCTCC
CCGGACCAGTTTGCATGTGCCACAGGGGAGATCGACTGTATCCCTGGGGCCTGGCGATGT
GATGGCTTTCCTGAATGCGATGACCAGAGTGACGAAGAGGGCTGCCCTGTGTGCTCTGCC
GCTCAGTTCCCCTGTGCGCGGGGTCAATGTGTTGACCTTCGCCTGAGATGTGACGGCGAA
GCAGACTGTCAGGACCGCTCAGACGAAGCGGACTGTGACGCCATCTGCCTGCCCAACCAG
TTCCGGTGTGCTAGCGGCCAGTGTGTCCTCATCAAACAGCAGTGCGACTCCTTCCCCGAC
TGTATCGACGGCTCCGACGAGCTCATGTGTGAAATTACCAAGCCGCCCTCAGACGACAGC
CCGGCCCACAGCAGTGCCATCGGGCCCGTCATTGGCATCATCCTCTCTCTCTTCGTCATG
GGTGGTGTCTATTTTGTGTGCCAGCGCGTGGTGTGCCAGCGCTATGCGGGGGCTAACGGG
CCCTTCCCGCACGAGTATGTTAGTGGGACCCCGCACGTGCCTCTCAATTTCATAGCTCCT
GGAGGTTCCCAGCATGGCCCCTTCACAGGAATCGCATGCGGAAAGTCCATGATGAGCTCT
GTGAGTCTGATGGGAGGCCGGGGCGGGGTGCCCCTCTACGATAGGAACCACGTTACAGGG
GCCTCGTCCAGCAGTTCTTCCAGCACGAAGGCCACATTATACCCTCCTATTCTGAACCCG
CCGCCCTCCCCGGCCACGGACCCCTCCCTGTACAACATGGACATGTTCTACTCTTCAAAC
ATTCCGGCCACTGCTAGACCGTACAGGCCTTACATCATTCGAGGAATGGCGCCCCCGACG
ACGCCCTGCAGCACCGACGTGTGTGACAGCGACTACAGCGCCAGTCGCTGGAAGGCCAGT
AAGTACTACCTGGATTTGAACTCGGACTCAGACCCCTATCCACCCCCACCCACGCCTCAC
AGCCAGTACCTTTCTGCGGAGGACAGCTGCCCGCCTTCTCCCGCTACCGAGAGGAGTTAC
TTTCATCTCTTCCCGCCCCCTCCGTCCCCCTGCACAGACTCATCC
Protein sequence predicted based on nucleotide sequence
MEAAPPGPPWPLLLLLLLLLALCGCPAPAAASPLLLFANRRDVRLVDAGGVKLESTIVVS GLEDAAAVDFQFSKGAVYWTDVSEEAIKQTYLNQTGAAVQNVVISGLVSPDGLACDWVGK KLYWTDSETNRIEVANLNGTSRKVLFWQDLDQPRAIALDPAHGYMYWTDWGETPRIERAG MDGSTRKIIVDSDIYWPNGLTIDLEEQKLYWADAKLSFIHRANLDGSFRQKVVEGSLTHP FALTLSGDTLYWTDWQTRSIHACNKRTGGKRKEILSALYSPMDIQVLSQERQPFFHTRCE EDNGGCSHLCLLSPSEPFYTCACPTGVQLQDNGRTCKAGAEEVLLLARRTDLRRISLDTP DFTDIVLQVDDIRHAIAIDYDPLEGYVYWTDDEVRAIRRAYLDGSGAQTLVNTEINDPDG IAVDWVARNLYWTDTGTDRIEVTRLNGTSRKILVSEDLDEPRAIALHPVMGLMYWTDWGE NPKIECANLDGQERRVLVNASLGWPNGLALDLQEGKLYWGDAKTDKIEVINVDGTKRRTL LEDKLPHIFGFTLLGDFIYWTDWQRRSIERVHKVKASRDVIIDQLPDLMGLKAVNVAKVV GTNPCADRNGGCSHLCFFTPHATRCGCPIGLELLSDMKTCIVPEAFLVFTSRAAIHRISL ETNNNDVAIPLTGVKEASALDFDVSNNHIYWTDVSLKTISRAFMNGSSVEHVVEFGLDYP EGMAVDWMGKNLYWADTGTNRIEVARLDGQFRQVLVWRDLDNPRSLALDPTKGYIYWTEW GGKPRIVRAFMDGTNCMTLVDKVGRANDLTIDYADQRLYWTDLDTNMIESSNMLGQERVV IADDLPHPFGLTQYSDYIYWTDWNLHSIERADKTSGRNRTLIQGHLDFVMDILVFHSSRQ DGLNDCMHNNGQCGQLCLAIPGGHRCGCASHYTLDPSSRNCSPPTTFLLFSQKSAISRMI PDDQHSPDLILPLHGLRNVKAIDYDPLDKFIYWVDGRQNIKRAKDDGTQPFVLTSLSQGQ NPDRQPHDLSIDIYSRTLFWTCEATNTINVHRLSGEAMGVVLRGDRDKPRAIVVNAERGY LYFTNMQDRAAKIERAALDGTEREVLFTTGLIRPVALVVDNTLGKLFWVDADLKRIESCD LSGANRLTLEDANIVQPLGLTILGKHLYWIDRQQQMIERVEKTTGDKRTRIQGRVAHLTG IHAVEEVSLEEFSAHPCARDNGGCSHICIAKGDGTPRCSCPVHLVLLQNLLTCGEPPTCS PDQFACATGEIDCIPGAWRCDGFPECDDQSDEEGCPVCSAAQFPCARGQCVDLRLRCDGE ADCQDRSDEADCDAICLPNQFRCASGQCVLIKQQCDSFPDCIDGSDELMCEITKPPSDDS PAHSSAIGPVIGIILSLFVMGGVYFVCQRVVCQRYAGANGPFPHEYVSGTPHVPLNFIAP GGSQHGPFTGIACGKSMMSSVSLMGGRGGVPLYDRNHVTGASSSSSSSTKATLYPPILNP PPSPATDPSLYNMDMFYSSNIPATARPYRPYIIRGMAPPTTPCSTDVCDSDYSASRWKAS KYYLDLNSDSDPYPPPPTPHSQYLSAEDSCPPSPATERSYFHLFPPPPSPCTDSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
