Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0022
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821053
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5151nts
Open reading frame: 1 to 5151
ATGGAACCGGCCGCCGCGGCAACCGTGCAAAGGCTGCCCGAACTCGGCAGGGAGGACAGA
GCTTCAGCTCCAGCTGCCGCCGCTGCTGCTGCTGCAGCAGCTGCAGCGGCTGCCGCGGCC
CTGGCTGCTGCAGCCGGTGGCGGTAGATCACCTGAGCCCGCCCTCACACCAGCTGCACCT
AGCGGAGGCAACGGCAGCGGAAGCGGCGCTAGAGAGGAGGCTCCTGGCGAGGCTCCCCCA
GGCCCCCTTCCCGGAAGGGCCGGTGGAGCTGGGAGAAGACGGAGAAGGGGCGCTCCCCAA
CCTATAGCTGGAGGGGCTGCCCCCGTCCCCGGAGCCGGCGGCGGGGCAAACAGCTTGCTG
TTAAGAAGAGGACGCCTGAAGAGGAACCTGAGTGCCGCGGCTGCTGCGGCCTCCAGCAGC
TCGTCTTCATCTGCTGCCGCTGCGAGCCACTCGCCCGGAGCTGCCGGCTTACCAGCCAGC
TGCTCCGCCAGCGCTAGCCTGTGTACCAGATCCCTGGATAGAAAGACCCTGCTCCTGAAG
CACAGACAGACACTCCAGTTGCAACCCAGCGACAGAGACTGGGTGAGGCATCAACTGCAG
AGAGGATGCGTGCATGTGTTTGACCGCCACATGGCTAGCACCTACCTTAGACCCGTCCTG
TGCACACTGGACACCACCGCTGGCGAAGTGGCCGCCCGCCTCCTGCAGCTTGGCCACAAG
GGCGGCGGCGTGGTGAAGGTGCTGGGACAAGGCCCCGGCGCTGCGGCTGCAAGAGAGCCC
GCTGAGCCTCCACCTGAGGCCGGGCCCAGACTTGCCCCACCCGAGCCCAGAGACTCTGAG
GTGCCACCCGCTAGAAGCGCACCTGGCGCCTTTGGCGGCCCTCCAAGGGCTCCGCCTGCC
GACCTGCCATTACCCGTGGGCGGACCCGGAGGGTGGAGCAGGAGAGCAAGCCCGGCTCCC
TCCGACAGCAGCCCTGGCGAACCCTTCGTGGGAGGACCCGTTTCCAGCCCCAGAGCCCCC
CGGCCCGTCGTCTCTGACACAGAGAGCTTCTCTCTGTCCCCCAGCGCCGAGAGCGTGTCC
GACAGACTGGACCCCTACTCCTCTGGCGGGGGCTCCTCCTCATCCAGCGAGGAGCTCGAG
GCCGACGCTGCCTCTGCTCCCACCGGAGTGCCTGGCCAGCCAAGAAGACCCGGCCATCCC
GCCCAGCCCTTACCCTTGCCTCAGACCGCCAGCTCTCCTCAGCCCCAACAGAAGGCCCCT
AGAGCCATCGATAGCCCCGGAGGCGCCGTGCGGGAGGGATCTTGCGAAGAGAAAGCCGCC
GCAGCCGTCGCTCCCGGCGGCCTGCAGTCCACCCCCGGTCGCTCTGGCGTTACCGCCGAG
AAAGCTCCTCCCCCTCCTCCGCCCCCAACACTGTACGTGCAGCTTCACGGCGAGACAACC
AGGAGACTTGAGGCTGAAGAGAAGCCCCTGCAGATCCAGAACGACTACCTTTTCCAGCTG
GGCTTCGGCGAGCTCTGGAGAGTGCAAGAGGAGGGCATGGATAGCGAGATCGGCTGCCTC
ATCAGATTCTACGCCGGCAAGCCCCACAGCACAGGCAGCTCTGAGAGAATCCAGCTTAGC
GGAATGTATAACGTGAGAAAGGGCAAAATGCAGTTGCCCGTCAACAGATGGACCAGAAGG
CAGGTCATCCTGTGCGGCACATGCCTGATCGTGAGCAGCGTGAAGGACTCCCTGACCGGC
AAGATGCACGTCCTGCCCCTCATCGGCGGAAAGGTTGAGGAGGTGAAGAAGCACCAGCAC
TGCCTCGCCTTCTCTAGCAGCGGACCACAGTCTCAGACCTACTACATTTGCTTCGACACC
TTTACCGAGTACCTGAGATGGCTCAGACAGGTGAGCAAGGTGGCCTCCCAGAGGATCAGC
TCTGTGGACCTTTCCTGCTGCTCTCTGGAGCATCTGCCCGCTAACCTGTTCTACAGCCAG
GACCTGACCCACCTTAATCTGAAGCAGAACTTCCTTAGACAGAACCCCAGCCTTCCTGCC
GCCAGGGGCCTCAACGAACTGCAAAGGTTCACCAAGCTCAAGAGCCTTAACCTGAGCAAC
AACCACCTTGGCGATTTCCCTCTGGCCGTCTGCAGCATCCCTACACTGGCCGAGCTGAAC
GTGAGCTGCAACGCCCTGAGAAGCGTGCCTGCCGCAGTGGGCGTCATGCACAACCTCCAG
ACCTTCCTTCTTGACGGCAATTTTCTGCAGTCTCTCCCTGCTGAACTGGAAAACATGAAG
CAGCTGAGCTATCTGGGCCTGAGCTTCAACGAGTTCACAGACATCCCCGAGGTTCTGGAG
AAGCTGACCGCCGTGGACAAGCTTTGCATGAGCGGAAACTGCGTGGAAACCTTGCGGCTT
CAGGCCCTTAGAAAGATGCCCCACATCAAACACGTGGACTTGAGACTGAACGTCATCAGA
AAGTTGATCGCCGACGAGGTGGACTTTCTCCAGCACGTGACACAGCTGGATCTCAGAGAC
AACAAGCTGGGCGACCTGGACGCTATGATCTTCAACAATATCGAGGTCCTGCACTGCGAG
AGAAACCAGCTCGTGACCCTGGACATCTGCGGCTACTTCCTGAAGGCCCTGTACGCCTCT
AGCAACGAGCTTGTGCAGCTCGACGTGTACCCCGTGCCCAACTACCTGAGCTACATGGAT
GTGAGCAGAAACAGATTGGAGAACGTCCCCGAGTGGGTGTGCGAGAGCAGGAAGCTGGAA
GTGCTCGACATCGGCCACAACCAGATCTGCGAACTGCCCGCCAGGCTGTTTTGCAACTCT
AGCCTGAGAAAACTTCTGGCTGGACACAACCAACTCGCCAGACTGCCTGAGAGGCTGGAG
AGAACCAGCGTCGAGGTGCTTGACGTGCAACATAACCAGTTGCTGGAGTTGCCCCCTAAC
CTGCTCATGAAGGCCGACAGCCTTAGATTCCTGAACGCCTCCGCCAATAAGCTCGAGAGC
TTACCCCCTGCCACCCTGTCCGAGGAAACCAACAGCATCCTCCAGGAGCTGTACCTCACC
AACAACTCCCTCACCGACAAGTGCGTCCCCCTGCTCACCGGCCACCCCCACCTGAAGATC
CTGCACATGGCCTACAACCGGCTCCAGTCCTTTCCCGCCAGCAAGATGGCCAAACTCGAG
GAACTGGAGGAGATTGACCTGTCAGGAAATAAGCTGAAAGCCATCCCCACCACCATCATG
AATTGCAGAAGAATGCACACCGTGATCGCCCACTCCAATTGCATTGAGGTGTTTCCCGAG
GTGATGCAGCTCCCCGAGATTAAGTGCGTGGACCTGTCCTGCAATGAGCTCAGCGAAGTG
ACACTGCCCGAGAACCTGCCCCCCAAGTTGCAGGAGCTTGATCTGACCGGAAACCCCAGA
CTGGTCCTGGACCACAAGACCCTTGAGCTGCTTAACAACATCAGGTGCTTCAAGATCGAC
CAGCCTAGCACCGGCGACGCTAGCGGCGCCCCCGCTGTGTGGAGCCACGGCTACACCGAG
GCCAGCGGCGTGAAGAACAAGTTGTGCGTGGCTGCCCTGTCAGTGAACAACTTCTGCGAC
AACAGAGAGGCTCTGTACGGCGTGTTCGACGGCGACAGAAACGTCGAAGTGCCCTACCTT
CTTCAGTGCACCATGAGCGACATCCTTGCTGAGGAACTCCAGAAGACCAAGAACGAGGAA
GAGTACATGGTGAACACCTTCATCGTGATGCAAAGGAAGCTCGGCACCGCCGGCCAGAAG
CTAGGAGGGGCCGCCGTGCTCTGTCACATCAAGCACGACCCCGTGGACCCCGGGGGAAGC
TTCACCCTGACCAGCGCCAACGTGGGCAAGTGCCAGACCGTCCTCTGCAGGAATGGCAAG
CCTCTGCCCCTGTCTAGAAGCTACATCATGAGCTGCGAAGAAGAGCTTAAGAGAATCAAG
CAGCACAAGGCCATCATCACCGAGGACGGCAAGGTGAACGGCGTCACCGAGAGCACCAGA
ATCCTGGGATACACCTTTCTGCATCCCTCAGTGGTGCCCAGACCCCACGTGCAGAGCGTG
CTGCTGACACCCCAGGACGAGTTCTTCATCCTGGGCAGCAAGGGACTGTGGGACTCCTTA
AGCGTGGAGGAAGCCGTGGAAGCAGTGAGGAACGTGCCCGACGCCTTGGCCGCTGCCAAA
AAGCTGTGCACCCTGGCCCAGAGCTACGGCTGCCACGACAGCATCTCTGCCGTGGTGGTC
CAACTGAGCGTGACCGAGGATTCTTTCTGTTGTTGCGAGCTGAGCGCCGGAGGTGCCGTC
CCTCCCCCCAGCCCCGGCATCTTCCCCCCTAGCGTGAACATGGTCATCAAGGACAGACCA
TCCGACGGCCTGGGCGTGCCTAGCTCCTCTAGCGGCATGGCTTCCGAGATTTCTAGCGAG
CTGTCCACCAGCGAGATGAGCTCCGAGGTGGGATCCACCGCCTCCGACGAACCCCCTCCC
GGGGCCCTGAGCGAGAACAGCCCCGCCTACCCCAGCGAGCAGCGATGCATGCTGCACCCC
ATCTGCCTCTCCAACAGCTTCCAGAGACAGCTCTCTTCCGCCACCTTCAGCAGCGCCTTT
AGCGACAACGGACTGGACAGCGACGACGAAGAGCCCATCGAGGGCGTCTTCACCAACGGA
AGCAGAGTGGAGGTCGAGGTCGACATCCATTGCAGCAGAGCCAAGGAGAAGGAAAAACAA
CAACACTTGCTCCAGGTGCCCGCCGAGGCCTCAGATGAGGGCATCGTTATCTCAGCCAAC
GAGGACGAGCCCGGACTGCCCAGAAAAGCCGACTTCAGCGCCGTGGGAACCATCGGAAGA
AGGAGAGCCAACGGCTCAGTGGCCCCCCAGGAGAGATCCCACAACGTGATCGAGGTGGCC
ACAGACGCTCCCCTCAGAAAGCCTGGAGGCTACTTTGCCGCCCCTGCCCAGCCTGACCCC
GACGACCAGTTCATCATCCCTCCTGAGTTGGAGGAGGAAGTGAAGGAGATCATGAAACAC
CACCAGGAACAACAGCAACAGCAGCAACCCCCCCCGCCCCCTCAGTTGCAGCCTCAGCTC
CCTAGACACTATCAGCTGGACCAGCTCCCAGACTACTACGACACCCCTCTG
Protein sequence predicted based on nucleotide sequence
MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAP SGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLL LRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLK HRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHK GGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPA DLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVS DRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAP RAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETT RRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLS GMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQH CLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQ DLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELN VSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLE KLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRD NKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMD VSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLE RTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLT NNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIM NCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPR LVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCD NREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQK LGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIK QHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSL SVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAV PPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPP GALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNG SRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGR RRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKH HQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
