Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0075
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821067
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6105nts
Open reading frame: 1 to 6105
ATGGCTTCGGCCGTGTCGCCCGCTAACTTGCCAGCGGTTCTTCTGCAGCCTCGCTGGAAG
CGAGTGGTGGGATGGTCGGGTCCGGTGCCACGGCCTCGCCACGGCCACCGCGCCGTGGCC
ATCAAGGAGCTTATCGTGGTGTTTGGCGGCGGCAACGAGGGGATAGTGGACGAACTTCAC
GTGTACAACACCGCAACCAACCAGTGGTTCATCCCAGCCGTGAGAGGGGACATTCCCCCT
GGGTGTGCAGCCTATGGATTCGTGTGTGACGGGACTCGCCTCCTGGTGTTTGGTGGGATG
GTGGAGTATGGGAAATACAGCAATGACCTCTACGAACTCCAGGCGAGCCGGTGGGAGTGG
AAGAGACTCAAAGCAAAGACGCCCAAAAACGGGCCCCCCCCGTGTCCTCGACTCGGGCAC
AGCTTCTCCCTTGTGGGAAACAAATGCTACCTGTTTGGGGGTCTGGCCAATGATAGTGAG
GACCCAAAGAACAACATTCCAAGATACCTGAATGACTTATATATCCTGGAACTACGGCCA
GGCTCTGGAGTGGTAGCCTGGGATATTCCCATCACTTACGGGGTCCTACCACCACCCCGG
GAGTCACATACTGCCGTGGTCTACACCGAAAAAGACAATAAGAAGTCCAAGCTTGTGATC
TACGGCGGGATGAGTGGCTGTAGGCTGGGGGACCTGTGGACCCTGGATATTGACACCCTG
ACGTGGAATAAGCCCAGTCTCAGCGGGGTGGCGCCTCTTCCTCGCAGTCTCCACTCGGCA
ACCACCATCGGAAATAAAATGTACGTGTTTGGAGGCTGGGTGCCTCTCGTCATGGATGAC
GTCAAAGTTGCCACACACGAGAAGGAATGGAAGTGTACCAACACGCTCGCTTGTCTCAAC
CTGGATACCATGGCCTGGGAAACCATCCTGATGGATACACTGGAGGACAACATCCCCCGT
GCCCGGGCTGGACACTGCGCAGTCGCCATCAACACCCGCCTGTACATTTGGAGTGGGCGT
GACGGCTACCGCAAGGCCTGGAACAACCAGGTCTGCTGCAAGGACCTCTGGTACCTAGAG
ACAGAAAAGCCACCACCCCCCGCCCGAGTACAACTGGTACGGGCCAACACCAACTCCCTG
GAGGTGAGCTGGGGTGCAGTGGCAACAGCCGACAGCTACCTTCTCCAGCTCCAGAAATAT
GACATTCCTGCCACGGCTGCTACTGCCACCTCCCCTACACCCAATCCGGTCCCATCTGTG
CCTGCCAACCCTCCCAAGAGCCCTGCACCAGCAGCAGCCGCCCCTGCTGTGCAGCCGCTG
ACCCAAGTAGGCATTACGCTTCTGCCCCAGGCTGCCCCCGCACCCCCGACCACCACCACC
ATCCAGGTCTTGCCAACCGTGCCTGGCAGCTCCATTTCTGTGCCCACCGCAGCCAGGACT
CAAGGTGTCCCTGCTGTTCTTAAAGTGACCGGTCCTCAGGCTACAACAGGAACTCCATTG
GTCACCATGCGACCTGCCAGCCAGGCTGGGAAAGCCCCTGTCACCGTGACCTCCCTTCCC
GCCGGAGTGCGGATGGTTGTGCCAACACAGAGTGCCCAGGGAACGGTGATTGGCAGTAGC
CCACAGATGAGTGGGATGGCCGCACTGGCCGCTGCGGCCGCTGCCACCCAGAAGATCCCC
CCTTCCTCGGCACCTACGGTGCTCAGTGTCCCAGCGGGTACCACCATCGTGAAGACCATG
GCTGTGACACCTGGGACTACCACTCTCCCAGCCACTGTGAAGGTGGCCTCCTCGCCAGTC
ATGGTGAGCAACCCTGCCACTCGCATGCTGAAGACTGCAGCCGCCCAGGTGGGGACATCG
GTTTCCTCCGCCACCAACACGTCTACCCGCCCTATCATCACAGTGCACAAGTCAGGCACT
GTGACCGTGGCTCAGCAAGCCCAAGTGGTGACCACAGTTGTGGGCGGGGTCACCAAGACC
ATCACTCTGGTGAAGAGCCCCATCTCTGTCCCAGGAGGCAGTGCTCTGATTTCCAATCTG
GGCAAAGTGATGTCGGTGGTGCAGACCAAGCCAGTTCAGACTTCAGCAGTCACAGGCCAG
GCGTCCACGGGTCCTGTGACTCAGATCATCCAGACCAAAGGGCCTCTGCCAGCGGGAACT
ATCCTGAAGCTGGTGACCTCAGCAGATGGCAAGCCCACCACCATTATCACTACCACGCAG
GCCAGTGGGGCGGGGACCAAGCCTACCATCCTGGGCATCAGTAGCGTTTCCCCCAGTACT
ACCAAGCCCGGCACGACCACCATCATCAAAACCATCCCCATGTCGGCCATCATCACCCAG
GCGGGCGCCACGGGAGTGACCAGCAGTCCTGGCATCAAGTCTCCCATTACCATCATTACC
ACCAAGGTCATGACTTCAGGAACTGGAGCCCCTGCGAAAATCATCACTGCTGTCCCCAAA
ATTGCCACCGGCCACGGGCAGCAGGGAGTGACCCAGGTGGTGCTTAAGGGGGCCCCGGGA
CAGCCAGGCACCATCCTCCGAACTGTGCCCATGGGAGGTGTTCGCCTGGTCACACCCGTC
ACCGTCTCTGCTGTCAAGCCTGCCGTCACCACGCTGGTTGTGAAAGGCACCACAGGTGTC
ACGACCCTAGGCACAGTTACAGGCACCGTCTCCACCAGCCTTGCCGGGGCAGGGGGCCAC
AGCACTAGTGCTTCCCTGGCCACGCCCATCACCACCTTGGGCACCATTGCCACCCTCTCA
AGCCAGGTGATCAACCCCACTGCCATCACCGTGTCGGCCGCACAGACAACGCTGACAGCG
GCAGGCGGGCTCACAACCCCAACCATCACCATGCAGCCCGTGTCCCAACCCACCCAGGTA
ACTCTGATCACCGCACCTAGTGGGGTGGAGGCCCAGCCTGTCCATGACCTACCTGTGTCC
ATTCTGGCCTCCCCGACTACAGAACAGCCCACCGCCACAGTTACCATCGCCGACTCAGGC
CAGGGTGATGTGCAACCTGGCACTGTCACCTTGGTGTGTTCCAATCCACCCTGTGAAACC
CACGAGACTGGCACCACCAACACGGCCACCACTACTGTTGTGGCTAACCTTGGGGGACAC
CCCCAGCCCACCCAAGTGCAGTTCGTCTGTGACAGACAAGAGGCAGCTGCTTCTCTTGTG
ACCTCGACTGTGGGCCAGCAGAATGGGAGCGTGGTCCGAGTCTGTTCGAACCCGCCCTGC
GAGACGCACGAGACGGGGACCACAAACACCGCTACCACCGCCACCTCCAACATGGCCGGG
CAGCATGGCTGCTCAAACCCACCCTGCGAGACACATGAGACGGGCACCACTAACACTGCC
ACTACAGCCATGTCGAGCGTCGGCGCCAACCACCAGCGAGATGCCCGACGGGCCTGTGCA
GCTGGCACCCCTGCCGTTATCAGGATCAGTGTGGCCACCGGGGCGCTGGAGGCAGCCCAG
GGCTCTAAGTCCCAGTGCCAAACCCGCCAGACCAGCGCGACCAGTACCACTATGACTGTG
ATGGCCACTGGGGCCCCGTGCTCCGCAGGCCCACTCCTAGGGCCTAGCATGGCTCGAGAG
CCCGGGGGCCGCAGCCCTGCTTTTGTCCAGTTGGCTCCTCTGTCCAGCAAAGTCAGGCTG
AGCAGCCCAAGCATTAAGGACCTTCCTGCGGGGCGCCACAGCCATGCGGTCAGCACCGCT
GCCATGACCCGTTCCAGCGTGGGTGCTGGTGAGCCCCGCATGGCACCTGTGTGCGAGAGC
CTCCAGGGTGGCTCGCCCAGCACTACAGTGACTGTGACAGCCCTGGAGGCACTGCTGTGC
CCCTCAGCCACAGTGACCCAAGTCTGTTCCAACCCCCCATGTGAAACTCACGAAACAGGC
ACAACCAACACCGCCACTACCTCGAATGCAGGCAGCGCCCAGAGGGTGTGCTCCAACCCT
CCATGCGAAACACACGAGACAGGCACTACCCACACGGCGACCACCGCTACTTCAAACGGG
GGCACGGGCCAGCCCGAGGGTGGGCAGCAGCCCCCTGCTGGTCGCCCCTGTGAGACACAC
CAGACCACTTCCACTGGAACCACCATGTCCGTCAGCGTGGGGGCCCTGCTTCCCGACGCC
ACTTCTTCCCACAGAACCGTCGAGTCTGGCCTAGAGGTGGCCGCGGCACCCAGCGTCACC
CCCCAAGCTGGCACCGCGCTGCTGGCTCCTTTCCCAACACAGAGAGTGTGCTCTAACCCC
CCCTGTGAGACTCACGAGACGGGAACCACTCACACGGCCACCACTGTCACTTCCAACATG
AGTTCAAACCAAGACCCCCCACCAGCTGCTAGCGATCAGGGAGAGGTGGAGAGCACCCAG
GGCGACAGCGTGAACATCACCAGCTCTAGTGCCATCACGACAACAGTGTCCTCCACTCTG
ACGCGCGCTGTGACCACCGTGACGCAGTCCACACCGGTCCCGGGCCCCTCTGTGCCTCCC
CCAGAGGAACTGCAGGTTTCGCCAGGTCCTCGCCAGCAGCTGCCGCCACGGCAGCTACTG
CAGTCGGCTTCCACAGCCCTGATGGGGGAGTCAGCCGAGGTCCTGTCAGCCTCTCAGACC
CCTGAGCTGCCGGCCGCCGTGGATCTGAGCAGCACAGGGGAGCCATCTTCGGGCCAGGAG
TCTGCCGGCTCTGCGGTGGTGGCCACTGTGGTGGTCCAGCCACCCCCACCTACACAGTCC
GAAGTAGACCAGTTATCACTTCCCCAAGAGCTAATGGCCGAGGCCCAAGCTGGGACCACC
ACCCTCATGGTAACGGGGCTGACCCCCGAGGAGCTGGCAGTGACGGCTGCTGCAGAAGCC
GCTGCCCAGGCCGCAGCTACAGAGGAAGCCCAGGCCCTCGCCATTCAGGCGGTGCTTCAG
GCCGCGCAGCAGGCTGTCATGGGCACCGGAGAGCCCATGGACACCTCCGAGGCAGCAGCA
ACCGTGACTCAGGCGGAGCTGGGGCACCTCTCTGCCGAGGGTCAGGAGGGCCAGGCCACC
ACCATACCCATTGTGCTGACACAGCAGGAGCTAGCTGCCCTGGTGCAGCAGCAGCAGCTG
CAGGAGGCCCAGGCCCAGCAGCAGCATCACCACCTCCCCACTGAGGCCCTGGCCCCTGCC
GACAGTCTCAACGACCCAGCCATTGAGTCCAATTGCCTCAATGAGCTGGCCGGCACGGTC
CCCAGCACGGTGGCGCTGCTGCCCTCAACGGCCACTGAGAGCCTGGCTCCATCCAACACA
TTTGTGGCCCCCCAGCCGGTTGTGGTGGCCAGCCCAGCCAAGCTGCAGGCTGCAGCTACC
CTTACCGAAGTGGCCAATGGCATCGAGTCCCTGGGTGTGAAGCCCGACCTGCCGCCCCCA
CCCAGCAAAGCCCCCATGAAGAAGGAAAACCAGTGGTTTGATGTGGGAGTCATTAAGGGC
ACCAATGTAATGGTGACACACTATTTCCTGCCACCAGATGATGCTGTCCCATCAGACGAT
GATTTGGGCACCGTCCCTGACTATAACCAGCTGAAGAAGCAGGAGCTGCAACCAGGCACA
GCCTATAAGTTTCGTGTAGCCGGCATCAATGCCTGTGGCCGGGGACCCTTCAGCGAAATC
TCAGCCTTTAAGACGTGCCTGCCTGGATTCCCAGGGGCCCCTTGTGCCATTAAAATCAGC
AAAAGTCCGGATGGTGCTCACCTGACCTGGGAGCCACCTTCTGTGACCTCCGGCAAGATT
ATCGAGTACTCCGTGTACCTGGCCATTCAGAGCTCACAGGCTGGGGGCGAGCTCAAGAGC
TCCACCCCGGCCCAGCTGGCCTTCATGCGGGTCTACTGCGGGCCCAGTCCCTCCTGTCTG
GTGCAGTCCTCCAGCCTTTCCAACGCCCACATCGACTACACCACCAAGCCCGCCATCATC
TTCCGCATCGCCGCCCGGAATGAGAAGGGCTATGGCCCGGCCACACAAGTGAGGTGGCTG
CAGGAAACCAGTAAAGACAGCTCTGGCACAAAGCCAGCCAACAAGCGGCCCATGTCCTCT
CCAGAAATGAAATCTGCTCCAAAGAAATCTAAGGCCGATGGTCAG
Protein sequence predicted based on nucleotide sequence
MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELH VYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEW KRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRP GSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTL TWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLN LDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLE TEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSV PANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAART QGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSS PQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPV MVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKT ITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGT ILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQ AGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPG QPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGH STSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQV TLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCET HETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPC ETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACA AGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMARE PGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCES LQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNP PCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDA TSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNM SSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPP PEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQE SAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEA AAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQAT TIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTV PSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPP PSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGT AYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKI IEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAII FRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
