Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0090
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821075
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6471nts
Open reading frame: 1 to 6471
ATGAGTGTGAAAGAAGCGGGAAGCTCGGGCCGCCGGGAGCAAGCGGCCTACCACCTTCAC
ATCTACCCCCAGCTGTCCACCACCGAGAGCCAGGCATCCTGCCGAGTGACTGCTACCAAG
GACTCCACAACCTCGGACGTGATCAAAGACGCCATTGCCTCACTGCGGCTGGACGGCACC
AAATGTTATGTGCTAGTGGAGGTCAAAGAGTCGGGAGGCGAGGAATGGGTGCTTGACGCC
AACGACTCGCCCGTCCACCGGGTCCTGCTATGGCCCCGCCGGGCACAGGACGAGCACCCT
CAGGAGGATGGATACTACTTCCTGCTGCAGGAACGCAACGCAGATGGAACCATCAAGTAC
GTGCACATGCAACTGGTGGCGCAGGCTACCGCCACCCGCCGCCTCGTGGAGCGGGGCCTC
CTGCCTCGGCAGCAGGCGGACTTTGATGACCTGTGTAACCTCCCCGAGCTAACCGAAGGC
AACCTCCTTAAGAACCTCAAGCATCGCTTCCTACAACAAAAGATCTACACGTACGCGGGG
AGCATTCTGGTGGCCATCAACCCCTTTAAGTTCCTGCCCATCTACAACCCCAAGTATGTC
AAGATGTATGAGAACCAGCAGTTGGGCAAGTTGGAGCCACACGTCTTCGCGCTGGCTGAC
GTGGCCTACTACACCATGCTTAGGAAACGCGTGAACCAGTGCATCGTGATCTCGGGTGAG
AGCGGCTCTGGCAAGACCCAGAGTACCAACTTCCTGATCCACTGCCTCACCGCCCTCAGT
CAGAAAGGCTACGCCAGCGGCGTCGAGAGAACCATCCTGGGTGCTGGCCCTGTTCTGGAG
GCTTTTGGAAACGCCAAGACAGCCCACAACAACAACTCGAGCAGGTTTGGGAAATTCATC
CAAGTCAGTTACCTAGAGAGTGGCATTGTGAGAGGAGCTGTCGTCGAGAAATATCTGCTT
GAAAAGTCTCGCCTGGTGTCTCAGGAGAAGGATGAAAGGAACTATCATGTGTTTTATTAT
TTGTTACTTGGAGTCAGCGAGGAGGAGCGCCAAGAATTTCAGCTCAAACAACCTGAAGAT
TATTTCTACCTCAACCAGCATAACTTGAAGATTGAAGACGGGGAGGACCTGAAGCATGAT
TTTGAAAGGCTCAAGCAGGCCATGGAGATGGTGGGCTTTCTCCCTGCCACGAAGAAGCAG
ATTTTTGCTGTCCTGTCGGCCATCCTGTACCTGGGAAACGTCACTTATAAGAAGAGAGCT
ACCGGCCGAGAAGAAGGGTTGGAGGTCGGGCCACCTGAGGTGCTCGACACCCTGTCGCAG
CTTCTGAAAGTGAAGCGAGAAATCCTGGTGGAGGTTCTGACCAAAAGAAAGACGGTCACC
GTCAACGACAAGCTTATCCTTCCCTACAGCCTGAGCGAGGCCATCACTGCCCGGGACTCT
ATGGCCAAGTCTCTCTATAGCGCCCTCTTCGACTGGATTGTCCTGCGGATCAACCACGCA
CTCCTCAACAAGAAGGACGTTGAAGAGGCAGTCTCGTGCCTATCCATTGGGGTCCTCGAC
ATCTTCGGGTTCGAAGACTTCGAGAGGAACAGCTTTGAGCAGTTTTGCATCAACTACGCC
AATGAACAGCTGCAGTATTACTTCAACCAACACATCTTCAAACTAGAGCAGGAGGAATAT
CAGGGAGAGGGGATCACGTGGCACAACATTGGCTACACCGACAATGTCGGCTGCATCCAT
CTCATCAGCAAGAAACCCACAGGCCTCTTCTACCTTCTGGACGAAGAAAGCAACTTTCCC
CACGCCACGAGCCAGACCTTACTGGCTAAGTTCAAACAGCAACATGAGGACAATAAGTAT
TTCCTGGGCACACCGGTCATGGAGCCCGCATTCATCATCCAGCACTTCGCAGGGAAGGTG
AAATATCAGATCAAGGACTTCCGGGAGAAGAACATGGACTACATGCGGCCAGACATCGTG
GCCCTCCTGCGGGGAAGTGACTCCTCCTACGTTCGGGAGCTCATCGGCATGGACCCCGTG
GCCGTCTTCCGGTGGGCCGTGCTCAGGGCCGCAATCCGGGCCATGGCAGTGCTTCGCGAG
GCCGGACGCCTGAGGGCCGAGAGAGCCGAAAAGGCTGCAGGTATGAGCAGCCCTGGTGCC
CAAAGTCACCCAGAAGAACTGCCAAGAGGCGCCAGCACACCCTCCGAAAAACTTTACCGC
GATTTGCATAACCAAATGATCAAGAGCATCAAAGGATTGCCCTGGCAAGGAGAGGATCCC
CGTAGCCTCCTTCAGTCTCTCAGTCGGCTCCAGAAACCCCGCGCCTTCATCCTGAAAAGT
AAAGGTATCAAACAAAAACAGATCATTCCAAAGAACCTACTGGACTCCAAATCCCTGAAA
CTCATCATTAGCATGACTCTGCACGACCGGACCACAAAGTCCCTACTCCACCTCCACAAA
AAGAAAAAACCACCTAGCATCAGCGCCCAATTCCAGACAAGTCTTAACAAGCTCTTGGAG
GCACTTGGGAAGGCGGAGCCCTTCTTTATCCGCTGCATTCGTTCCAATGCAGAAAAGAAG
GAACTGTGCTTCGACGACGAGCTGGTCTTGCAGCAGCTCCGCTACACAGGCATGCTGGAG
ACAGTGCGAATCAGGAGGTCAGGGTACAGTGCCAAGTACACGTTCCAGGACTTCACAGAG
CAGTTCCAGGTGCTTCTGCCCAAGGATGCCCAGCCTTGCAGAGAGGTGATCTCCACCCTC
CTGGAGAAAATGAAGATAGACAAGCGCAATTACCAGATTGGGAAGACCAAGGTGTTCCTG
AAAGAAACGGAGCGGCAAGCCCTGCAGGAGACACTGCACCGGGAGGTGGTGCGGAAAATC
CTGTTGCTGCAGAGCTGGTTCCGGATGGTGCTGGAGCGTCGGCACTTCCTCCAGATGAAA
AGGGCCGCTGTGACCATTCAGGCCTGTTGGCGGTCCTACAGGGTCCGCAGGGCGCTGGAG
AGGACGCAGGCTGCCGTGTACCTCCAGGCTTCATGGAGAGGCTACTGGCAGCGGAAGCTC
TACCGGCACCAGAAACAGAGTATCATCCGCCTACAGAGCCTGTGTCGGGGGCATCTGCAG
CGTAAGTCATTCTCCCAGATGATCTCTGAGAAGCAAAAGGCAGAAGAGAAAGAACGTGAA
GCCCTGGAGGCCGCAAGAGCGGGTGCTGAGGAGGGCGGACAGGGACAGGCGGCTGGTGGG
CAACAGGTAGCTGAGCAGGGACCGGAGCCAGCCGAGGATGGCGGGCACCTGGCATCGGAG
CCCGAGGTGCAGCCAAGTGACAGGTCACCCCTAGAGCACTCTTCGCCTGAGAAAGAGGCC
CCAAGCCCAGAGAAAACTCTGCCTCCCCAGAAAACCGTGGCGGCTGAAAGTCACGAGAAA
GTCCCCAGCAGCCGCGAGAAACGGGAGTCGCGGCGGCAAAGAGGGCTGGAACACGTCAAG
TTTCAGAACAAACACATCCAGTCCTGCAAGGAGGAGAGTGCCCTCCGCGAACCTTCTAGA
CGGGTCACCCAAGAGCAAGGGGTGAGTCTGCTGGAAGATAAGAAAGAGAGCAGAGAAGAT
GAAACCCTTTTAGTGGTAGAGACGGAGGCTGAGAACACCTCCCAAAAGCAGCCCACCGAG
CAACCCCAGGCTATGGCAGTTGGCAAGGTTTCTGAAGAAACTGAGAAGACGTTGCCCAGT
GGGAGCCCCAGGCCCGGCCAGTTAGAGCGGCCGACAAGCCTCGCCCTGGACAGTAGAGTC
AGCCCTCCGGCCCCCGGCAGCGCCCCTGAGACTCCCGAAGACAAAAGCAAACCATGTGGC
AGCCCAAGGGTTCAGGAAAAGCCCGACAGCCCCGGTGGGTCCACGCAGATCCAGCGGTAC
CTGGACGCCGAGCGGTTGGCCAGCGCCGTCGAACTGTGGCGGGGCAAGAAGCTGGTGGCA
GCTGCTAGCCCTAGTGCCATGCTCAGCCAGTCTCTGGACCTCAGCGACAGACACCGTGCC
ACAGGGGCCGCACTCACGCCCACAGAGGAGAGGCGCACCTCATTCTCTACGAGCGACGTG
TCCAAGCTCCTGCCGTCCCTGGCTAAAGCTCAACCTGCCGCAGAAACCACGGACGGAGAA
CGAAGTGCGAAAAAGCCGGCTGTCCAGAAAAAGAAGCCAGGCGACGCATCCTCCCTCCCA
GACGCAGGGCTGTCCCCGGGCTCTCAGGTCGACTCTAAATCCACGTTTAAGAGGCTCTTT
CTGCACAAAACCAAGGATAAAAAATACAGCCTCGAGGGAGCAGAGGAACTGGAGAATGCC
GTGTCCGGGCACGTCGTGCTTGAAGCCACCACAATGAAGAAAGGCCTGGAAGCTCCCTCC
GGACAGCAGCATCGCCACGCTGCTGGTGAGAAGCGGACCAAGGAACCAGGAGGCAAAGGG
AAGAAAAACCGAAATGTCAAAATTGGCAAGATCACAGTGTCAGAGAAGTGGCGGGAATCG
GTGTTCCGCCAGATCACCAACGCTAATGAGCTCAAGTACCTCGACGAATTCCTGCTCAAT
AAGATAAATGACCTGCGTTCACAGAAGACGCCTATTGAGAGTTTGTTTATCGAAGCTACC
GAGAAGTTCAGGAGCAACATCAAAACGATGTACTCTGTCCCGAATGGGAAGATCCACGTG
GGCTACAAGGATCTGATGGAGAACTACCAGATCGTCGTCAGCAACCTGGCTACTGAGCGT
GGCCAGAAGGACACCAACCTGGTCCTCAACCTCTTCCAATCACTCCTAGATGAGTTCACC
CGCGGCTACACAAAGAACGACTTTGAACCTGTGAAGCAGAGCAAAGCTCAGAAGAAGAAG
CGCAAGCAGGAGCGTGCTGTCCAAGAACACAACGGGCATGTGTTCGCCAGCTACCAGGTT
AGCATTCCGCAGTCGTGCGAGCAGTGCCTCTCTTATATCTGGCTCATGGACAAGGCCCTG
CTGTGCAGCGTGTGCAAGATGACATGCCACAAGAAGTGCGTGCACAAGATTCAGAGTCAC
TGCTCCTACACCTACGGGAGGAAGGGCGAGCCAGGAGTTGAGCCTGGCCACTTCGGCGTG
TGCGTAGACAGTCTGACCAGCGACAAAGCCTCTGTGCCCATCGTGTTGGAGAAACTGCTA
GAACACGTGGAGATGCACGGACTGTACACCGAGGGCCTCTACCGCAAATCCGGTGCTGCC
AACCGAACTCGGGAACTCCGGCAAGCGCTCCAGACAGACCCCGCAGCAGTCAAGCTCGAG
AACTTCCCCATCCACGCCATCACAGGGGTTCTGAAGCAGTGGCTGCGGGAGCTGCCCGAG
CCACTCATGACCTTCGCACAGTACGGCGATTTCCTCCGAGCCGTTGAGCTGCCGGAGAAA
CAGGAGCAGCTCGCTGCCATCTATGCCGTCCTGGAGCACCTTCCTGAAGCCAACCACAAC
TCCCTGGAGAGACTCATCTTCCACCTGGTCAAGGTGGCCTTGCTCGAGGATGTCAACCGC
ATGTCACCTGGGGCGCTCGCCATTATCTTCGCACCCTGCCTCCTCCGTTGCCCCGACAAC
TCAGACCCGCTGACCAGTATGAAGGATGTCCTCAAGATTACTACGTGCGTGGAAATGCTT
ATCAAGGAGCAGATGAGAAAATACAAGGTGAAGATGGAGGAGATCAGCCAACTCGAAGCC
GCAGAGTCTATCGCCTTTCGAAGGCTGTCGCTTCTGCGACAAAATGCCCCCTGGCCTCTC
AAACTGGGCTTCTCGAGTCCCTATGAGGGGGTCCTGAACAAGAGTCCCAAGACCCGGGAC
ATCCAAGAGGAAGAGCTGGAGGTGCTGCTGGAGGAGGAAGCAGCCGGCGGCGATGAGGAC
CGGGAAAAGGAGATTCTCATTGAACGGATCCAATCCATCAAGGAAGAGAAGGAGGACATT
ACCTACCGGCTCCCGGAGCTGGACCCAAGGGGCTCGGACGAGGAGAACCTGGACTCGGAG
ACGTCGGCTAGCACCGAAAGCCTGCTAGAGGAACGGGCTGGTCGGGGCGCATCGGAAGGT
CCCCCTGCGCCTGCTCTCCCTTGTCCTGGCGCGCCTACCCCATCTCCCCTTCCCACAGTG
GCCGCCCCTCCACGACGAAGGCCGTCGTCCTTCGTAACGGTCAGAGTGAAGACACCCCGG
CGGACCCCCATCATGCCCACGGCTAACATCAAGCTCCCACCAGGTTTGCCCTCACATCTG
CCTCGCTGGGCACCGGGTGCTAGGGAAGCGGCTGCCCCAGTGCGGCGACGGGAACCCCCT
GCTCGCCGCCCGGACCAGATACATTCCGTGTACATCACGCCCGGGGCAGACCTGCCAGTG
CAAGGCGCCCTTGAGCCCCTCGAAGAAGATGGCCAGCCACCTGGCGCCAAGCGGAGGTAC
TCGGATCCCCCCACGTACTGCCTGCCCCCCGCCTCGGGCCAGACCAATGGC
Protein sequence predicted based on nucleotide sequence
MSVKEAGSSGRREQAAYHLHIYPQLSTTESQASCRVTATKDSTTSDVIKDAIASLRLDGT KCYVLVEVKESGGEEWVLDANDSPVHRVLLWPRRAQDEHPQEDGYYFLLQERNADGTIKY VHMQLVAQATATRRLVERGLLPRQQADFDDLCNLPELTEGNLLKNLKHRFLQQKIYTYAG SILVAINPFKFLPIYNPKYVKMYENQQLGKLEPHVFALADVAYYTMLRKRVNQCIVISGE SGSGKTQSTNFLIHCLTALSQKGYASGVERTILGAGPVLEAFGNAKTAHNNNSSRFGKFI QVSYLESGIVRGAVVEKYLLEKSRLVSQEKDERNYHVFYYLLLGVSEEERQEFQLKQPED YFYLNQHNLKIEDGEDLKHDFERLKQAMEMVGFLPATKKQIFAVLSAILYLGNVTYKKRA TGREEGLEVGPPEVLDTLSQLLKVKREILVEVLTKRKTVTVNDKLILPYSLSEAITARDS MAKSLYSALFDWIVLRINHALLNKKDVEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYA NEQLQYYFNQHIFKLEQEEYQGEGITWHNIGYTDNVGCIHLISKKPTGLFYLLDEESNFP HATSQTLLAKFKQQHEDNKYFLGTPVMEPAFIIQHFAGKVKYQIKDFREKNMDYMRPDIV ALLRGSDSSYVRELIGMDPVAVFRWAVLRAAIRAMAVLREAGRLRAERAEKAAGMSSPGA QSHPEELPRGASTPSEKLYRDLHNQMIKSIKGLPWQGEDPRSLLQSLSRLQKPRAFILKS KGIKQKQIIPKNLLDSKSLKLIISMTLHDRTTKSLLHLHKKKKPPSISAQFQTSLNKLLE ALGKAEPFFIRCIRSNAEKKELCFDDELVLQQLRYTGMLETVRIRRSGYSAKYTFQDFTE QFQVLLPKDAQPCREVISTLLEKMKIDKRNYQIGKTKVFLKETERQALQETLHREVVRKI LLLQSWFRMVLERRHFLQMKRAAVTIQACWRSYRVRRALERTQAAVYLQASWRGYWQRKL YRHQKQSIIRLQSLCRGHLQRKSFSQMISEKQKAEEKEREALEAARAGAEEGGQGQAAGG QQVAEQGPEPAEDGGHLASEPEVQPSDRSPLEHSSPEKEAPSPEKTLPPQKTVAAESHEK VPSSREKRESRRQRGLEHVKFQNKHIQSCKEESALREPSRRVTQEQGVSLLEDKKESRED ETLLVVETEAENTSQKQPTEQPQAMAVGKVSEETEKTLPSGSPRPGQLERPTSLALDSRV SPPAPGSAPETPEDKSKPCGSPRVQEKPDSPGGSTQIQRYLDAERLASAVELWRGKKLVA AASPSAMLSQSLDLSDRHRATGAALTPTEERRTSFSTSDVSKLLPSLAKAQPAAETTDGE RSAKKPAVQKKKPGDASSLPDAGLSPGSQVDSKSTFKRLFLHKTKDKKYSLEGAEELENA VSGHVVLEATTMKKGLEAPSGQQHRHAAGEKRTKEPGGKGKKNRNVKIGKITVSEKWRES VFRQITNANELKYLDEFLLNKINDLRSQKTPIESLFIEATEKFRSNIKTMYSVPNGKIHV GYKDLMENYQIVVSNLATERGQKDTNLVLNLFQSLLDEFTRGYTKNDFEPVKQSKAQKKK RKQERAVQEHNGHVFASYQVSIPQSCEQCLSYIWLMDKALLCSVCKMTCHKKCVHKIQSH CSYTYGRKGEPGVEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAA NRTRELRQALQTDPAAVKLENFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEK QEQLAAIYAVLEHLPEANHNSLERLIFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDN SDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEISQLEAAESIAFRRLSLLRQNAPWPL KLGFSSPYEGVLNKSPKTRDIQEEELEVLLEEEAAGGDEDREKEILIERIQSIKEEKEDI TYRLPELDPRGSDEENLDSETSASTESLLEERAGRGASEGPPAPALPCPGAPTPSPLPTV AAPPRRRPSSFVTVRVKTPRRTPIMPTANIKLPPGLPSHLPRWAPGAREAAAPVRRREPP ARRPDQIHSVYITPGADLPVQGALEPLEEDGQPPGAKRRYSDPPTYCLPPASGQTNG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
