Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0093
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821077
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6603nts
Open reading frame: 1 to 6603
ATGGGGGCCATGACTCAGCTGTTGGCGGGTGTCTTTCTTGCTTTCCTTGCCCTCGCTACC
GAAGGTGGGGTGCTCAAGAAAGTCATCCGGCACAAGCGACAGAGTGGGGTGAACGCCACC
CTGCCAGAAGAGAACCAGCCAGTCGTTTTTAACCACGTTTACAACATCAAGCTGCCAGTG
GGATCCCAGTGTTCGGTGGATCTGGAGTCAGCCAGTGGGGAGAAAGACCTGGCACCGCCT
TCAGAGCCCAGCGAGAGCTTTCAGGAGCACACAGTAGATGGGGAAAACCAGATTGTCTTC
ACACATCGGATCAACATCCCCCGCCGGGCCTGTGGCTGTGCCGCAGCCCCTGATGTTAAG
GAGCTACTTAGCAGACTTGAGGAGCTGGAGAACCTGGTGTCTTCCCTGAGGGAGCAATGT
ACTGCAGGAGCAGGCTGCTGTCTCCAGCCTGCCACAGGCCGCTTGGACACCAGGCCCTTC
TGTAGCGGTCGGGGCAACTTCAGCACTGAAGGATGTGGCTGTGTCTGCGAACCCGGCTGG
AAAGGCCCCAACTGCTCTGAGCCCGAATGTCCAGGCAACTGTCACCTTCGAGGCCGGTGC
ATTGATGGGCAGTGCATCTGTGACGACGGCTTCACCGGCGAGGACTGCAGCCAGCTGGCC
TGCCCCAGCGACTGCAATGACCAGGGCAAGTGCGTAAATGGAGTCTGCATCTGCTTCGAA
GGCTACGCCGGGGCTGACTGCAGTCGTGAAATCTGCCCAGTGCCCTGCAGTGAGGAGCAC
GGCACATGTGTAGATGGCTTGTGTGTGTGCCACGATGGGTTTGCAGGCGATGACTGCAAC
AAGCCTCTGTGTCTCAACAATTGCTATAATCGTGGACGATGCGTGGAGAATGAGTGCGTG
TGTGATGAGGGTTTCACGGGCGAAGACTGCAGTGAGCTCATCTGCCCCAATGACTGCTTC
GACCGGGGACGCTGCATCAATGGCACCTGTTACTGCGAAGAAGGCTTCACAGGTGAAGAC
TGCGGGAAACCCACTTGCCCACATGCCTGCCACACCCAGGGCCGGTGTGAGGAGGGGCAG
TGTGTCTGTGATGAGGGCTTCGCCGGTGTGGACTGCTCAGAGAAGAGGTGTCCTGCTGAC
TGTCACAATCGTGGCCGCTGTGTAGACGGGCGGTGTGAGTGTGATGATGGTTTCACTGGA
GCTGACTGTGGCGAGCTCAAGTGTCCCAATGGCTGCAGTGGTCATGGACGCTGTGTAAAT
GGGCAGTGCGTGTGTGATGAGGGCTATACTGGGGAGGACTGCAGCCAGCTACGGTGCCCC
AATGACTGTCACAGCCGGGGCCGCTGTGTCGAAGGCAAATGTGTATGTGAGCAAGGCTTC
AAGGGCTATGACTGCTCCGACATGAGCTGCCCTAATGACTGTCACCAGCACGGCCGGTGT
GTTAATGGCATGTGTGTTTGTGATGACGGCTACACAGGGGAAGACTGCCGGGATCGCCAA
TGCCCCAGGGACTGCAGCAACCGCGGCCTCTGTGTGGACGGACAGTGCGTCTGTGAGGAC
GGCTTCACCGGCCCTGACTGTGCAGAACTCTCCTGTCCAAATGACTGCCATGGCCAGGGT
CGCTGTGTGAATGGGCAGTGCGTGTGCCATGAAGGATTTATGGGCAAAGACTGCAAGGAG
CAAAGATGTCCCAGTGACTGTCATGGCCAGGGCCGCTGCGTGGACGGCCAGTGCATCTGC
CACGAGGGCTTCACAGGCCTGGACTGTGGCCAGCACTCCTGCCCCAGTGACTGCAACAAC
TTAGGACAATGCGTCTCCGGCCGCTGCATCTGCAACGAGGGCTACAGCGGAGAAGACTGC
TCAGAGGTGTCTCCTCCCAAAGACCTCGTTGTGACTGAAGTGACCGAAGAGACGGTCAAC
CTGGCCTGGGACAATGAGATGCGGGTCACAGAGTACCTTGTCGTGTACACGCCCACACAC
GAGGGTGGTCTGGAAATGCAATTCCGTGTGCCTGGGGATCAGACGTCCACCATCATCCAG
GAGCTGGAGCCTGGTGTGGAGTACTTTATCCGTGTATTTGCCATCCTGGAGAACAAGAAG
AGCATTCCTGTCAGCGCCAGGGTAGCCACGTACTTACCTGCACCTGAAGGCCTGAAATTC
AAGTCCATCAAGGAGACATCTGTAGAAGTGGAGTGGGATCCTCTAGACATTGCTTTTGAA
ACCTGGGAGATCATCTTCCGGAATATGAATAAAGAAGATGAGGGAGAGATCACCAAAAGC
CTGCGGAGGCCAGAGACTTCTTACCGGCAAACTGGTCTAGCGCCCGGGCAAGAGTATGAG
ATATCTCTGCACATAGTGAAAAACAATACCCGGGGCCCTGGCCTGAAGAGGGTGACCACC
ACACGCTTGGATGCCCCCAGCCAGATCGAGGTGAAAGATGTCACAGACACCACTGCCTTG
ATTACCTGGTTCAAGCCGCTGGCTGAGATCGATGGCATTGAGCTGACATACGGCATCAAA
GACGTGCCAGGCGACCGGACCACCATCGATCTCACAGAGGACGAGAACCAGTACTCCATC
GGGAACCTGAAGCCTGACACTGAGTACGAGGTGTCCCTCATCTCCCGCAGAGGTGACATG
TCAAGCAACCCAGCCAAAGAGACATTCACAACAGGCCTGGATGCTCCCAGGAATCTTCGA
CGTGTTTCCCAGACTGATAACAGCATCACCCTGGAATGGAGGAATGGCAAGGCAGCTATT
GACAGTTACAGAATTAAGTATGCCCCCATCTCTGGAGGGGACCACGCTGAGGTTGATGTT
CCAAAGAGCCAACAAGCCACAACCAAAACCACACTCACAGGTCTGAGGCCGGGAACTGAA
TATGGGATTGGAGTTTCTGCAGTTAAGGAAGACAAGGAGAGCAATCCAGCGACCATCAAC
GCAGCCACAGAGTTGGACACGCCCAAGGACCTTCAGGTTTCTGAGACTGCAGAGACTAGC
CTGACCCTGCTCTGGAAAACACCGTTAGCCAAATTTGACCGCTACCGCCTCAATTATAGT
CTCCCCACAGGCCAGTGGGTCGGAGTGCAACTTCCAAGAAACACCACTTCCTATGTCCTG
AGGGGCCTGGAACCAGGACAGGAGTACAATGTCCTTCTGACAGCCGAGAAAGGCAGACAC
AAGAGCAAGCCCGCACGGGTGAAGGCATCCACTGAACAAGCCCCTGAGCTGGAAAACCTC
ACCGTGACTGAGGTTGGGTGGGATGGCCTCAGACTAAACTGGACCGCAGCTGATCAGGCC
TATGAGCACTTTATCATTCAGGTTCAGGAGGCCAACAAGGTGGAGGCAGCTCGGAACCTC
ACCGTGCCTGGCAGCCTTCGGGCAGTGGACATACCGGGCCTGAAGGCTGCTACGCCTTAT
ACAGTCTCCATCTATGGGGTGATCCAGGGCTATAGAACACCAGTGCTCTCTGCTGAGGCC
TCCACTGGGGAAACTCCCAATCTGGGAGAGGTCGTGGTGGCCGAGGTTGGCTGGGATGCC
CTCAAACTCAACTGGACTGCTCCAGAAGGGGCCTATGAGTACTTTTTCATTCAGGTGCAG
GAGGCTGACACAGTCGAGGCAGCCCAGAATCTCACCGTCCCTGGAGGACTGAGGTCCACA
GACCTGCCTGGGCTCAAAGCAGCCACTCATTATACCATCACCATCCGCGGGGTCACTCAG
GACTTCAGCACAACCCCTCTCTCTGTTGAAGTCTTGACAGAGGAGGTGCCAGATATGGGA
AACCTCACAGTGACCGAGGTTAGCTGGGATGCGCTCAGGCTGAACTGGACCACGCCAGAT
GGAACCTATGACCAGTTTACTATTCAGGTCCAGGAGGCTGACCAGGTGGAAGAAGCTCAC
AATCTCACGGTTCCTGGCAGCCTGCGTTCCATGGAAATCCCAGGCCTTAGGGCTGGCACT
CCTTACACAGTCACCCTGCATGGCGAGGTCAGGGGCCACAGCACTCGACCCCTTGCCGTA
GAGGTCGTCACAGAGGATCTCCCACAGCTGGGAGATTTAGCCGTGTCTGAGGTTGGCTGG
GATGGCCTCAGACTTAACTGGACCGCAGCTGACAATGCCTATGAGCACTTTGTCATTCAA
GTGCAGGAGGTCAACAAAGTGGAGGCAGCACAGAACCTCACGTTGCCTGGCAGCCTCAGG
GCTGTGGACATCCCGGGCCTCGAGGCTGCCACGCCTTATAGAGTCTCCATCTATGGGGTG
ATCCGGGGCTATAGAACACCAGTACTCTCTGCTGAGGCCTCAACAGCCAAAGAACCTGAA
ATTGGAAACTTAAATGTTTCAGACATTACTCCCGAGAGCTTCAATCTCTCCTGGATGGCG
ACCGATGGGATCTTCGAGACTTTTACCATTGAAATTATTGATTCCAATAGGTTGCTGGAG
ACTGTTGAATATAATATCTCTGGTGCCGAACGAACTGCCCATATCTCGGGGCTACCCCCC
AGTACTGACTTTATTGTCTACCTCTCTGGACTTGCTCCCAGCATCCGGACCAAAACCATT
AGTGCCACAGCCACGACAGAGGCCCTGCCCCTTCTGGAAAACCTAACCATTTCCGACATT
AATCCTTACGGATTCACAGTTTCCTGGATGGCATCGGAGAATGCCTTTGACAGCTTTCTA
GTAACGGTGGTGGATTCTGGGAAGCTACTGGACCCCCAGGAATTCACACTTTCAGGAACC
CAGAGGAAGCTAGAGCTGAGAGGCCTCATCACTGGCATTGGCTATGAGGTTATGGTGTCT
GGCTTCACCCAAGGGCATCAAACCAAGCCCTTGAGGGCTGAGATTGTTACAGAAGCCGAA
CCGGAAGTTGACAACCTCCTGGTTTCAGATGCCACCCCAGACGGTTTCCGTCTGTCCTGG
ACAGCTGATGAAGGCGTGTTCGACAATTTTGTTCTCAAAATCAGAGATACCAAAAAGCAG
TCTGAGCCACTGGAAATAACCCTACTTGCCCCCGAACGTACTAGAGACATAACAGGACTC
AGAGAGGCTACTGAATACGAAATTGAACTCTATGGCATAAGCAAGGGAAGGCGATCCCAG
ACAGTCAGTGCTATAGCAACCACAGCAATGGGCTCCCCAAAGGAAGTCATTTTCTCAGAC
ATCACTGAAAATTCGGCTACTGTCAGCTGGAGAGCACCCACAGCACAAGTGGAGAGCTTC
CGGATTACCTATGTGCCTATCACAGGCGGTACACCTTCCATGGTAACTGTGGACGGAACC
AAGACTCAGACCAGGCTGGTGAAACTCATACCTGGCGTGGAGTATCTTGTTAGCATCATT
GCCATGAAGGGCTTTGAGGAAAGTGAACCTGTCTCAGGGTCATTCACCACAGCTCTGGAT
GGCCCATCTGGCCTGGTTACAGCCAACATTACTGACTCAGAAGCCTTGGCCAGGTGGCAG
CCAGCCATTGCCACTGTGGACAGTTATGTCATCTCTTACACAGGCGAGAAAGTGCCAGAA
ATTACACGGACGGTGTCCGGGAACACAGTGGAGTATGCTCTGACCGATCTCGAGCCTGCC
ACGGAATACACACTGAGAATCTTTGCAGAGAAAGGGCCCCAGAAGAGCTCAACCATCACT
GCCAAGTTCACAACAGACCTCGATTCTCCAAGAGACTTGACTGCTACTGAGGTTCAGTCG
GAAACTGCCCTCCTTACCTGGCGACCCCCCCGGGCATCAGTCACAGGTTACCTGCTGGTC
TATGAATCAGTGGATGGCACAGTCAAGGAAGTCATTGTGGGTCCAGATACCACCTCCTAC
AGCCTGGCGGACCTGAGCCCATCCACCCACTACACAGCCAAGATCCAGGCGCTCAATGGG
CCCCTGAGGAGCAATATGATCCAGACCATCTTCACCACAATTGGACTCCTGTACCCCTTC
CCCAAGGACTGCTCCCAAGCAATGCTGAATGGTGACACGACCTCCGGCCTGTACACCATT
TATCTGAATGGCGATAAGGCTGAGGCGCTGGAAGTCTTCTGTGACATGACCTCTGATGGG
GGTGGATGGATTGTGTTCCTGAGACGCAAGAACGGACGCGAGAACTTCTACCAAAACTGG
AAGGCATATGCTGCTGGATTTGGGGACCGCAGAGAAGAATTCTGGCTTGGGCTGGACAAC
CTGAACAAAATCACAGCCCAGGGGCAGTACGAGCTGCGGGTGGACCTGCGGGACCATGGG
GAGACAGCATTTGCTGTCTATGACAAGTTCAGCGTGGGAGATGCCAAGACTCGCTACAAG
CTGAAGGTGGAGGGATACAGTGGGACAGCCGGTGACTCCATGGCCTACCACAATGGCAGA
TCCTTCTCCACCTTTGACAAGGACACAGATTCAGCCATCACCAACTGTGCTCTGTCCTAC
AAAGGGGCTTTCTGGTATAGGAACTGTCACCGAGTCAACCTGATGGGGAGATATGGGGAC
AATAACCACAGTCAGGGCGTCAATTGGTTCCACTGGAAGGGCCATGAACACTCTATCCAG
TTTGCTGAGATGAAGCTGCGGCCAAGTAACTTCAGAAATCTTGAAGGCAGGCGCAAAAGG
GCA
Protein sequence predicted based on nucleotide sequence
MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIKLPV GSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVK ELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVCEPGW KGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNGVCICFE GYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRGRCVENECV CDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACHTQGRCEEGQ CVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKCPNGCSGHGRCVN GQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDCSDMSCPNDCHQHGRC VNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDGFTGPDCAELSCPNDCHGQG RCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQCICHEGFTGLDCGQHSCPSDCNN LGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTEETVNLAWDNEMRVTEYLVVYTPTH EGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAILENKKSIPVSARVATYLPAPEGLKF KSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYE ISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVTDTTALITWFKPLAEIDGIELTYGIK DVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRRGDMSSNPAKETFTTGLDAPRNLR RVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHAEVDVPKSQQATTKTTLTGLRPGTE YGIGVSAVKEDKESNPATINAATELDTPKDLQVSETAETSLTLLWKTPLAKFDRYRLNYS LPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLLTAEKGRHKSKPARVKASTEQAPELENL TVTEVGWDGLRLNWTAADQAYEHFIIQVQEANKVEAARNLTVPGSLRAVDIPGLKAATPY TVSIYGVIQGYRTPVLSAEASTGETPNLGEVVVAEVGWDALKLNWTAPEGAYEYFFIQVQ EADTVEAAQNLTVPGGLRSTDLPGLKAATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMG NLTVTEVSWDALRLNWTTPDGTYDQFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGT PYTVTLHGEVRGHSTRPLAVEVVTEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQ VQEVNKVEAAQNLTLPGSLRAVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPE IGNLNVSDITPESFNLSWMATDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPP STDFIVYLSGLAPSIRTKTISATATTEALPLLENLTISDINPYGFTVSWMASENAFDSFL VTVVDSGKLLDPQEFTLSGTQRKLELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAE PEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDITGL REATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSATVSWRAPTAQVESF RITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALD GPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPA TEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYLLV YESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPF PKDCSQAMLNGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNW KAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYK LKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGD NNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
