Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-011.0044
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821099
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4017nts
Open reading frame: 1 to 4017
ATGGCAAAGCGTAGGCCTCACAAACATCCTGGCCGTGAAGAGCTTGTCTTCAAGAGTTCC
TGGTGCAGGAACTCGCCCGTGGCCACCCAGGAGCCTGATGTGCAAGGAAGGTGTGAGTGC
CAGGACGGAATTCCTGGAGATGAAGGCCTGGAGCTGCGGTCTGCGGACTCGGCAGTGCCC
GTGGCCATGACCCAGGCTGCCGTGCGGCCTTGGGCACCCTGCCTGGAGAACATGACCACT
GCCCCAAACGGCCTCGGGCCCGGCCCCGCAGCCCCCTGTGCAGGCTCCGACCTCAAAGAC
GCCAAAATGGTGACCTCCCTCGCGTGTGGAAATGGAGTCTGTGGCTGCAGCCCTGGTGGC
GACACGGACACCCAGGAAGCCAAACTGAGCCCAGCCAAGCTTGTGCGCCTCTTTTCCACC
AGCCGGAAGAGGACGGGTGCCCACCCCGAGCGGCCCCACTCCATGGTCCTCGTGGGGAAC
TCCTCCACATGGAACACCCTCGCCTCCTTCCGGAAAATGGGATCCTTTAAGAAACTGAAG
AGCTCCGTGCTGAAAGGGATTCAGAGCCGAGAGGGGTCAAATGCCTGTTCTAAGGGAGAG
GCTTCGGAGCATGGCCTGGGAAAGTCCATCCCAAATGGCGCTGTCCCAGGAGCCCAGGCC
AGCAGGGGCTCCCCCTTAGCACCGGGACCAGCATGTGGTGCCCTCAGGCCAGCAGAGTGG
GGCACATTGGATGGCTCCGACCTGGAGGACACGGACGATGCCTTCCAGCGGAGCACACAC
CGCTCCCGGAGCCTCCGGAGAGCCTACGGCCTGGGACGCATCTGCCTGCTGGATGCGCCC
CAGAACCATGCGACACCCACGATAGCCACAGGCCAGGTGCCCGCCGTCTGTGAGATTCTC
GTGAGGGACCCTGAAAACAACAGCATGGGCTACAGGAGGAGCAAGAGCACGGACAATCTT
GCCTTTCTGAAGAAGAGCTCCTTTAAGCGGAAGTCCACCTCCAATCTTGCCGACCTCAGG
ACGGCCCATGACGCACGGGTACCACAGAGGACCCTGAGTAGCTCCTCCACTGACTCCCAA
AAGCTTGGGTCAGGAAGGACAAAACGCTGGAGGAGCCCGATAAGGGCCAAGGACTTTGAC
AGAGTCTTCAAACTTGTCAGCAATGTGACTGAGGCTGCCTGGAGGAGGGAGAGCCCTAGA
AGCGGGGCCCCATCCCCTGGAGAGGCCAGCCTGAGACTTCAGGCACACAGCCGGCTGCAT
GACGACTACTCCCGCCGCGTCTCCAGGAGCACTGAGCAGGACAGCAGGCGGGGCGGGGCG
GTCATGCATGGGACCACTGCAACCTGCACCGTGGCCCCCGGTTTCGGCTCAGCCACCTCT
AAGGGGCCCCACCTAGACGCAGACACTGCCGTATTTCCTCTTGAAACCAAAAGTTCCTGG
GCGGTGGAAAGCGACAGTTCCTGCACTTGCAGCTCTCTGCCAAGCCCGATTGTCCAGGAT
GTGTTGAGCAAAGACTCCTGTGACCCAAACGCCGGCAGCCAGTTAACATTTGACCCTGAG
CAGCCTCCCACCCCTCTGAGGCCCACCACACCCAAGCCCCAGAGCCCTCAAAGCCCCCAG
AGCCCCGGGGCAGGATCTGCCAGCTGTCACAGCAATCACAGTGCCCTGTCCGCGAATTCA
GAGGAAAGTGAAGGAAGGGCAGAAGAGCCTGCTCAGAGAGAGCCAGGGCCCGTGTCCTTG
CAGGATCCCCTGGAAGCTACACATGGTGATGAGGGCAGCAAGGACCTTCTGGTGAACATT
GGTGTGGCAGCCGGCCCAGAAGAAAAGGAGAAGGAGGAGGTCGTCCCTGATGGCCCCTGG
AGGCGAAGCTCATCACAGGATGAGGAAAGGACAGAGGCACAGAGAACCCCCAAGAGGAGA
TGGGGCTCTGGGAGACGGCCAAGGCCTCGGCCATTCTCTGACTACGGCCAGCTGGCCAGC
CGGAGTCTGTCTATTCCTGAAGACTCTGTTGCTGCAGACCCCCAGAAGGAAGACCGGGTG
GACGAGGACCCCCAGGCAAGCATGACTTCTGCCAGCCCTGAAGACCAGAATGCTCCAGTG
GGCTGCCCCAAAGGAGCCCGGAGAAGGCGCCCCATTTCTGTGATAGGAGGGGTCAGCTTG
TATGGGACCAACCAGACGGAGGAACTGGACAATCTTCTGACCCAACCGGCTTCCAGGCCG
CCCATGCCTGCTCACCAGGTGCCACCCTACAAGGCTGTGTCGGCCCGGTTCCGGCCCTTC
ACATTCTCCCAGAGCACCCCCATTGGGTTGGACCGTGTGGGACGCCGGCGGCAGATGAGA
GCATCCAACGTTTCTTCAGATGGAGGTACTGAGCCCTCTGCCTTAGTGGATGACAACGGT
AGTGAGGAGGACTTCAGCTATGAAGACCTCTGCCAGGCCAGCCCTCGGTACCTGCAGCCC
GGCGGGGAGCAGCTGGCCATCAATGAGCTGATCAGTGATGGCAACGTGGTCTGCGCAGAA
GCCCTGTGGGACCATGTGACCATGGATGACCAGGAACTGGGCTTCAAAGCCGGGGATGTC
ATCCAGGTTCTGGAAGCCTCCAACAAGGACTGGTGGTGGGGCCGCAGTGAAGATAAGGAA
GCCTGGTTCCCCGCGAGCTTCGTCAGATTGCGAGTGAATCAGGAAGAGCTGTCGGAAAAC
TCCAGCAGCACCCCCAGTGAGGAGCAGGACGAGGAGGCCAGCCAGAGCCGCCACAGACAC
TGTGAGAACAAGCAGCAGATGCGGACCAACGTCATCCGGGAGATCATGGACACCGAGCGG
GTGTACATCAAACACCTCAGGGACATCTGTGAGGGCTATATCCGACAGTGCCGCAAGCAC
ACAGGAATGTTCACCGTTGCGCAGCTAGCCACAATTTTTGGAAACATTGAAGATATTTAC
AAATTCCAAAGGAAGTTTCTTAAAGACCTTGAGAAACAGTACAACAAAGAGGAACCTCAC
TTAAGTGAAATAGGATCTTGCTTTCTTCAAAATCAAGAGGGCTTTGCCATCTATTCCGAG
TACTGCAACAACCACCCGGGCGCCTGCCTGGAGCTGGCCAACCTCATGAAGCAGGGCAAG
TACAGACATTTCTTTGAAGCCTGCCGCCTGCTGCAGCAGATGATTGACATCGCCATCGAC
GGGTTCCTGCTCACACCAGTGCAGAAGATCTGCAAATACCCGCTGCAGCTGGCCGAGCTG
CTCAAGTATACCACACAGGAACACGGTGATTACTCAAACATAAAGGCAGCATATGAGGCC
ATGAAGAATGTGGCCTGTCTGATCAACGAGCGCAAGCGCAAGCTGGAGAGCATCGACAAG
ATCGCTCGCTGGCAGGTTTCTATCGTGGGCTGGGAGGGACTGGATATCTTAGACAGAAGC
TCAGAATTGATTCATTCTGGGGAGCTGACCAAAATCACTAAGCAAGGCAAAAGCCAGCAG
CGGACGTTCTTCCTGTTTGACCACCAGCTGGTGTCCTGCAAGAAGGACCTGCTGCGGAGG
GACATGCTGTACTACAAGGGCAGGCTGGACATGGATGAGATGGAGCTTGTGGACCTGGGG
GATGGGCGCGACAAGGACTGCAACCTCAGCGTGAAAAATGCCTTCAAGCTCGTCAGCAGA
ACCACAGACGAGGTTTATTTGTTTTGTGCCAAAAAACAAGAAGACAAGGCGAGGTGGCTG
CAGGCATGTGCAGATGAAAGGAGGCGGGTGCAAGAGGACAAGGAGATGGGAATGGAAATT
TCAGAAAACCAGAAGAAACTTGCCATGTTAAATGCTCAAAAGGCAGGACATGGAAAGTCA
AAAGGCTACAACAGGTGCCCTGTGGCCCCACCGCACCAGGGCCTTCATCCCATCCACCAG
CGCCACATCACTATGCCCACAAGCGTCCCCCAGCAGCAGGTCTTTGGCCTGGCGGAACCC
AAGAGGAAGTCCTCGCTCTTCTGGCACACCTTCAACAGGCTCACCCCCTTCCGGAAA
Protein sequence predicted based on nucleotide sequence
MAKRRPHKHPGREELVFKSSWCRNSPVATQEPDVQGRCECQDGIPGDEGLELRSADSAVP VAMTQAAVRPWAPCLENMTTAPNGLGPGPAAPCAGSDLKDAKMVTSLACGNGVCGCSPGG DTDTQEAKLSPAKLVRLFSTSRKRTGAHPERPHSMVLVGNSSTWNTLASFRKMGSFKKLK SSVLKGIQSREGSNACSKGEASEHGLGKSIPNGAVPGAQASRGSPLAPGPACGALRPAEW GTLDGSDLEDTDDAFQRSTHRSRSLRRAYGLGRICLLDAPQNHATPTIATGQVPAVCEIL VRDPENNSMGYRRSKSTDNLAFLKKSSFKRKSTSNLADLRTAHDARVPQRTLSSSSTDSQ KLGSGRTKRWRSPIRAKDFDRVFKLVSNVTEAAWRRESPRSGAPSPGEASLRLQAHSRLH DDYSRRVSRSTEQDSRRGGAVMHGTTATCTVAPGFGSATSKGPHLDADTAVFPLETKSSW AVESDSSCTCSSLPSPIVQDVLSKDSCDPNAGSQLTFDPEQPPTPLRPTTPKPQSPQSPQ SPGAGSASCHSNHSALSANSEESEGRAEEPAQREPGPVSLQDPLEATHGDEGSKDLLVNI GVAAGPEEKEKEEVVPDGPWRRSSSQDEERTEAQRTPKRRWGSGRRPRPRPFSDYGQLAS RSLSIPEDSVAADPQKEDRVDEDPQASMTSASPEDQNAPVGCPKGARRRRPISVIGGVSL YGTNQTEELDNLLTQPASRPPMPAHQVPPYKAVSARFRPFTFSQSTPIGLDRVGRRRQMR ASNVSSDGGTEPSALVDDNGSEEDFSYEDLCQASPRYLQPGGEQLAINELISDGNVVCAE ALWDHVTMDDQELGFKAGDVIQVLEASNKDWWWGRSEDKEAWFPASFVRLRVNQEELSEN SSSTPSEEQDEEASQSRHRHCENKQQMRTNVIREIMDTERVYIKHLRDICEGYIRQCRKH TGMFTVAQLATIFGNIEDIYKFQRKFLKDLEKQYNKEEPHLSEIGSCFLQNQEGFAIYSE YCNNHPGACLELANLMKQGKYRHFFEACRLLQQMIDIAIDGFLLTPVQKICKYPLQLAEL LKYTTQEHGDYSNIKAAYEAMKNVACLINERKRKLESIDKIARWQVSIVGWEGLDILDRS SELIHSGELTKITKQGKSQQRTFFLFDHQLVSCKKDLLRRDMLYYKGRLDMDEMELVDLG DGRDKDCNLSVKNAFKLVSRTTDEVYLFCAKKQEDKARWLQACADERRRVQEDKEMGMEI SENQKKLAMLNAQKAGHGKSKGYNRCPVAPPHQGLHPIHQRHITMPTSVPQQQVFGLAEP KRKSSLFWHTFNRLTPFRK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
