Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-004.0118
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821119
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3519nts
Open reading frame: 1 to 3519
ATGAGAGCAAAAGAGGCTGCGGAAACGGGACTGCGGCCACTACTTCCGGCGTGTACCGAG
AGACTGGCGTCCGGCGTGTACCGAGAGACTGGCGTCCGGTGTGCCGGTGGCCACATGGAT
CCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACCTGACTGACCCC
TCTTATGGAATCCCCCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTTACGCGGGCGCAC
GTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTGGGCCTCGCGGTGCAGGCCATACCT
CCTTTTGAATTTGCCTTCAAAGATGAGCGGATCTCTTTTACTATTCTGGATGCCGTGATC
AGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGAGGCCAATGTTTATCCAGCAGAG
TGCCGGGGCCGGAGGAGTACCTACCGTGGCAAATTGACAGCTGATATTAACTGGGCAGTG
AATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATGGTGAAA
TCCAAGCTTTGCAACTTGCGTAACCTTCCACCACAAGCTCTCATTGAGCACCATGAGGAG
GCAGAGGAAATGGGCGGCTATTTTATAATCAATGGAATTGAAAAAGTCATCCGAATGTTG
ATTATGCCTCGGAGAAATTTTCCCATTGCTATGATAAGACCAAAATGGAAAACCAGAGGG
CCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTGAGGGAAGAACATTCCGCCGTC
AATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTGAACTTTATTTACCGGAAA
GAACTGTTCTTTCTTCCTTTGGGATTTGCACTCAAGGCACTGGTCAGCTTTTCTGATTAT
CAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAACTCTGTT
TCCCAGATGTTAAGGATTGTAATGGAAGAGGGTTGCTCGACACAAAAACAGGTCCTTAAC
TACCTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAA
GCTGCGGAGTTCCTGTTTAACCAATGCATCTGTATCCACTTGAAATCCAATACTGAAAAG
TTTTATATGCTTTGTCTCATGACGCGAAAGCTCTTTGCCTTAGCGAAAGGAGAGTGCATG
GAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACACCGGGTCAGCTCTTCCTT
ATGTTCCTGAAGGAAAAACTCGAAGGTTGGTTAGTGTCTATTAAAATAGCTTTTGATAAG
AAGGCTCAGAAGACCAGTGTTTCTATGAACACAGACAATTTGATGAGGATTTTTACAATG
GGCATAGACCTTACCAAACCATTTGAATACCTTTTTGCTACTGGGAATCTGCGTTCTAAA
ACAGGTCTTGGCCTCCTACAAGATTCTGGACTGTGTGTTGTGGCTGACAAGCTGAACTTC
ATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCTGATTTTGCCAAGATGAGG
ACCACCACAGTACGCAGACTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCCCGTGCATACC
CCCGACGGGGAGCCCTGTGGCCTGATGAACCACCTAACTGCCGTGTGTGAGGTTGTCACA
CAGTTTGTGTATACGGCATCTATTCCAGCTTTATTGTGCAACTTGGGGGTCACTCCCATT
GATGGAGCTCCCCACCGATCTTACAGTGAGTGCTACCCTGTCCTGCTGGACGGTGTCATG
GTTGGCTGGGTGGATAAGGATCTTGCCCCAGGCATCGCAGATTCACTTCGTCATTTTAAG
GTGTTGCGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTGATACCCATGACAGGA
AAACCCAGTCTCTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTCGTACGGCCT
GTGCAGAACTTAGCATTGGGCAAAGAAGAGCTCATTGGAACTATGGAACAGATCTTCATG
AATGTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTGACCACACACCAGGAACTCTTT
CCACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCTTTCTCTGATCACAACCAGAGT
CCTCGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATGGGCTTTCCACTTCTCACC
TATCAAGACCGATCGGATAACAAACTGTATCGGCTTCAGACTCCTCAGAGTCCCTTGGTT
AGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAATGCCATC
GTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAATAAGGCC
TCTTGGGAACGAGGGTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCT
GAAAAAATTAAACAAGGAGATAGTAGCCTCGTGTTTGGCATCAAACCTGGTGACCCACGC
GTTCTGCAGAAGTTAGATGACGACGGATTGCCCTTTATAGGCGCAAAACTGCAGTACGGA
GATCCGTATTACAGTTACCTCAACCTCAACACCGGGGAAAGCTTTGTGATGTACTATAAG
AGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGTAATGACACTGGGAGTGGA
AAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACTATCGGAGATAAA
TTTGCCAGTCGGCATGGGCAGAAGGGCATTTTGAGCAGATTGTGGCCGGCTGAGGACATG
CCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTTCCATCC
CGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGT
CTGTGCCATGATGCTACACCCTTCATCTTCTCGGAGGAGAACTCGGCCTTGGAATACTTT
GGTGAGATGTTAAAGGCCGCTGGCTACAATTTCTATGGCACCGAGAGGTTATATAGTGGC
ATCAGTGGGCTGGAACTGGAAGCAGACATCTTCATAGGCGTGGTTTATTATCAGCGCTTA
CGCCATATGGTTTCAGACAAATTTCAAGTAAGGACAACTGGAGCCCGAGACAGAGTCACC
AACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAGATGGAACGG
GATGCGCTGTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAACTGCTCA
GATAGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTCTTGGAG
AAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGGAAATACAACTGTACTCTGTGTAGT
CGGAGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTAGCTGAA
CTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTT
Protein sequence predicted based on nucleotide sequence
MRAKEAAETGLRPLLPACTERLASGVYRETGVRCAGGHMDPGSRWRNLPSGPSLKHLTDP SYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFKDERISFTILDAVI SPPTVPKGTICKEANVYPAECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIMVK SKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRG PGYTQYGVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDY QIFQELIKGKEDDSFLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQ AAEFLFNQCICIHLKSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFL MFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSK TGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRRLLPESWGFLCPVHT PDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPVLLDGVM VGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRP VQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQS PRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAI VAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPR VLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSG KFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPS RMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSG ISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMER DALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCS RSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
