Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-004.0163
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821135
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4125nts
Open reading frame: 1 to 4125
ATGGAGGGGGACCGGGTGGCCGGGCGGCCGGTGCTGTCGTCGTTACCAGTGCTACTCCTC
CTCCCGTTGCTAATGCTCCGGGCCGCCGCGCTGCACCCAGACGAGCTCTTCCCACACGGG
GAGTCGTGGGGCGACCAGCTCCTGCAGGAGGGCGACGACGAAAGCTCCGCCGTGGTGAAG
CTGGCGAATCCCCTCCACTTCTACGAAGCCAGATTCAGCAACCTCTACGTGGGCACCAAC
GGCATCATCTCCACTCAGGACTTCCCCAGAGAAACGCAGTATGTGGACTATGATTTCCCC
ACCGACTTCCCGGCCATCGCCCCTTTTCTGGCGGACATCGACACCAGCCACGGCAGAGGC
CGAGTCCTGTACCGAGAGGACACCTCCCCCGCAGTGCTGGGCCTGGCCGCCCGCTATGTG
CGCGCTGGCTTCCCGCGCTCTGCGCGCTTTACCCCTACCCACGCCTTCCTGGCCACCTGG
GAGCAGGTAGGCGCTTACGAGGAGGTCAAGCGCGGGGCGCTGCCTTCGGGAGAGCTGAAC
ACTTTCCAGGCAGTGTTGGCATCTGATGGGTCCGATAGCTACGCCCTCTTTCTTTATCCT
GCCAACGGCTTGCAGTTCCTTGGAACCCGCCCCAAAGAGTCTTACAATGTCCAGCTTCAG
CTTCCAGCTCGGGTGGGCTTCTGCCGAGGGGAGGCTGATGATCTGAAGTCAGAAGGACCA
TATTTCAGCTTGACTAGCACTGAACAGTCTGTGAAAAATCTGTATCAACTAAGCAACCTG
GGGATCCCTGGAGTGTGGGCTTTCCATATCGGCAGCACTTCCCCGTTGGACAATGTGAGG
CCAGCTGCAGTTGGCGACCTTTCCGCTGCCCACTCTTCTGTTCCTCTGGGACGTTCCTTC
AGCCATGCCACAGCCCTGGAAAGTGACTATAATGAGGACAATTTGGATTACTATGATGTG
AATGAGGAGGAAGCTGAATACCTTCCGGGTGAACCAGAGGAGGCACTGAATGGCCACAGC
AGCATTGATGTTTCCTTCCAATCCAAAGTGGATACAAAGCCTTTAGAGGAATCTTCCACC
TTGGATCCTCACACCAAAGAGGGAACATCTCTGGGAGAGGTAGGGGGCCCAGATTTAAAA
GGCCAAGTTGAGCCCTGGGATGAGAGAGAAACCAGAAGCCCAGCTCCACCAGAGGTAGAC
AGAGATTCACTGGCTCCTTCCTGGGAAACCCCACCACCGTACCCCGAAAACGGAAGCATC
CAGCCTTACCCAGATGGAGGGCCCGTGCCTTCGGAAATGGATGTTCCCCCAGCTCATCCT
GAAGAAGAAATTGTTCTGCGAAGTTACCCTGCTTCAGGTCACACTACACCTTTAAGTCGA
GGCACGTATGAGGTGGGACTGGAAGATAACATAGGTTCCAACACCGAGGTGTTCACGTAT
AATGCCGCCAACAAGGAAACCTGTGAACACAACCACAGACAATGCTCCCGGCATGCATTC
TGCACGGACTATGCCACTGGCTTCTGCTGCCACTGCCAATCCAAGTTTTATGGAAATGGA
AAGCACTGTCTGCCTGAAGGGGCACCTCACCGAGTGAATGGGAAAGTGAGTGGCCACCTC
CACGTGGGCCATACACCAGTGCACTTCACTGATGTCGACCTGCATGCGTATATCGTGGGC
AATGATGGCAGAGCCTACACGGCCATCAGCCACATCCCACAGCCCGCAGCCCAGGCCCTC
CTCCCCCTCACACCAATTGGAGGCCTGTTTGGGTGGCTCTTTGCCTTAGAAAAACCTGGC
TCTGAGAACGGCTTCAGCCTCGCCGGTGCTGCCTTTACCCATGACATGGAAGTTACATTC
TACCCGGGCGAGGAGACGGTTCGTATCACTCAAACTGCTGAGGGACTTGACCCTGAGAAC
TACCTGAGCATTAAGACCAACATTCAAGGCCAGGTGCCTTACGTGTCAGCAAATTTCACA
GCCCACATCTCTCCCTACAAGGAGCTGTACCACTACTCCGACTCCACTGTGACCTCTACA
AGTTCCAGAGACTACTCTCTGACTTTTGGTGCAATCAACCAAACATGGTCCTACCGGATC
CACCAGAACATCACTTACCAGGTGTGCCGGCACGCCCCCAGACACCCGTCCTTCCCCACC
ACCCAGCAGCTGAACGTGGACCGGGTGTTTGCCTTGTATAATGACGAAGAAAGAGTGCTT
AGATTTGCTGTGACCAATCAAATTGGCCCGGTCAAAGAGGATTCAGACCCCACTCCGGGG
AATCCTTGCTATGATGGGAGCCACATGTGTGACACAACAGCACGGTGCCATCCAGGGACA
GGTGTAGATTACACCTGTGAGTGCGCATCCGGGTACCAGGGAGATGGACGGAACTGTGTG
GATGAAAATGAATGTGCAACTGGCTTTCATCGGTGTGGCCCCAACTCTGTATGTATCAAC
TTGCCTGGAAGCTACAGGTGTGAGTGCCGGAGTGGTTATGAGTTTGCAGATGACCGGCAT
ACTTGCATCTTGATAACCCCCCCTGCCAACCCCTGTGAGGATGGAAGTCATACCTGTGCT
CCTGCTGGGCAGGCCAGGTGTGTTCACCATGGAGGCAGCACGTTCAGCTGTGCCTGCCTC
CCCGGTTATGCCGGCGATGGGCACCAGTGCACTGATGTAGATGAGTGCTCAGAAAACAGA
TGTCACCCTGCAGCTACCTGCTACAATACTCCTGGTTCCTTCTCCTGCCGTTGTCAACCC
GGATATTATGGCGATGGATTTCAGTGCATACCTGACTCCACCTCAAGCCTGACACCTTGT
GAACAACAGCAGCGCCATGCCCAGGCCCAGTATGCCTACCCCGGGGCCAGGTTCCACATT
CCCCAATGCGACGAGCAGGGCAACTTCCTGCCCCTACAGTGTCATGGCAGCACTGGTTTC
TGCTGGTGCGTGGACCCTGATGGTCATGAAGTTCCTGGTACCCAGACTCCACCTGGCTCC
ACCCCCCCTCACTGTGGACCATCACCAGAGCCCACCCAGAGGCCCCCGACCATCTGTGAG
CGCTGGAGGGAAAACCTGCTGGAGCACTACGGTGGCACCCCCCGGGATGACCAGTACGTG
CCCCAGTGCGATGACCTGGGCCACTTCATCCCCCTGCAGTGCCACGGAAAGAGCGACTTC
TGCTGGTGTGTGGACAAAGATGGCAGAGAGGTGCAGGGCACCCGCTCTCAGCCCGGCACC
ACCCCTGCGTGTATACCCACCGTCGCTCCACCCATGGTCCGGCCCACGCCCCGCCCAGAT
GTGACCCCTCCATCTGTGGGCACCTTCCTGCTCTATACCCAGGGCCAGCAGATTGGCTAC
TTACCCCTCAATGGCACCAGGCTTCAGAAGGATGCAGCTAAGACCCTGCTGTCTCTCCAT
GGCTCCATAATCGTGGGAATTGATTACGACTGCCGGGAGAGGATGGTGTACTGGACAGAT
GTTGCTGGACGGACAATCAGCCGTGCCGGTCTTGAACTGGGAGCAGAGCCTGAGACGATT
GTGAATTCAGGTCTGATAAGCCCTGAAGGACTTGCCATAGACCACATCCGCAGAACAATG
TACTGGACGGACAGTGTGCTGGATAAGATAGAGAGCGCCCTGCTTGATGGCTCTGAGCGC
AAGGTCCTCTTCTACACAGATCTGGTGAATCCCCGTGCCATCGCAGTGGATCCAATCCGA
GGCAACTTGTACTGGACAGACTGGAATAGAGAAGCCCCTAAAATTGAAACGTCTTCTTTA
GATGGAGAAAACAGAAGGATTCTGATCAATACAGACATTGGATTGCCCAATGGCTTAACC
TTTGACCCTTTCTCTAAACTGCTCTGCTGGGCAGATGCAGGAACCAAAAAACTGGAGTGT
ACACTCCCTGATGGAACTGGACGGCGTGTCATTCAAAACAACCTCAAGTACCCCTTCAGC
ATCGTAAGCTATGCAGATCACTTCTACCACACAGACTGGAGGAGGGATGGTGTTGTATCA
GTAAATAAACATTCTGGCCAGTTTACTGATGAGTATCTCCCCGAACAACGATCCCACCTC
TACGGGATAACTGCAGTGTACCCCTACTGCCCAACAGGAAGGAAG
Protein sequence predicted based on nucleotide sequence
MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVK LANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRG RVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELN TFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGP YFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSF SHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESST LDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSI QPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTY NAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHL HVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPG SENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFT AHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPT TQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGT GVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRH TCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENR CHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHI PQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICE RWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGT TPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLH GSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTM YWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSL DGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFS IVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
