Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-004.0186
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821145
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4548nts
Open reading frame: 1 to 4548
ATGGCTCCCTACCCATGTGGATGCCACATCCTGCTGCTGCTCTTTTGCTGCCTGGCTGCT
GCGAGGGCTAACCTGCTTAACCTGAACTGGCTTTGGTTTAACAATGAGGACACTAGCCAT
GCAGCTACAACAATACCCGAGCCCCAAGGACCACTTCCTGTGCAGCCTACAGCAGATACC
ACCACACACGTGACTCCCAGGAATGGTTCTACAGAACCTGCTACAGCTCCTGGCAGCCCT
GAACCACCCTCAGAGCTGTTAGAAGATGGACAGGACACACCCACTTCTGCTGAGAGCCCT
GACGCTCCAGAAGAGAACATTGCCGGTGTCGGTGCCGAGATCTTGAACGTGGCTAAAGGA
ATCCGAAGCTTTGTTCAGCTGTGGAATGACACTGTCCCTACTGAGAGCTTGGCCAGAGCT
GAAACACTTGTACTGGAGACTCCTGTGGGACCCCTTGCCCTTGCGGGACCTTCTAGCACC
CCCCAAGAGAATGGGACAACTCTCTGGCCTAGCCGTGGAATACCTAGCTCTCCTGGCGCC
CATACAACTGAGGCTGGCACCTTGCCTGCACCCACTCCATCACCTCCCTCCCTGGGAAGG
CCTTGGGCACCACTCACAGGACCATCAGTACCTCCACCATCTTCAGAGAGAATCAGCGAG
GAGGTGGGGCTGTTGCAGTTATTAGGGGACCCTCCTCCCCAGCAGGTCACCCAGACAGAT
GATCCCGACGTTGGACTGGCTTACGTCTTTGGGCCAGATGCCAATAGCGGCCAAGTGGCC
AGATATCACTTCCCTAGCCTATTCTTCCGTGATTTCTCACTTTTGTTCCACATCCGGCCA
GCTACAGAGGGACCAGGGGTTCTTTTCGCCATTACAGACTCGGCACAGGCCATGGTTTTA
CTGGGAGTGAAGCTATCTGGGGTACAGGACGGGCATCAGGACATCTCCCTACTTTACACA
GAGCCAGGTGCAGGACAGACACATACAGCCGCCTCATTTCGGCTACCCGCCTTCGTTGGA
CAGTGGACACACTTGGCATTATCTGTGGCTGGTGGCTTTGTGGCTCTCTACGTTGATTGT
GAGGAGTTTCAGAGAATGCCGCTAGCTCGATCCTCACGGGGCTTAGAGCTGGAGCCGGGC
GCCGGGCTATTCGTGGCTCAGGCTGGGGGAGCAGATCCTGATAAGTTTCAGGGGGTGATC
GCCGAACTAAAGGTGCGCAGGGATCCACAGGTGAGTCCTATGCACTGCCTTGATGAGGAA
GGTGATGATTCAGATGGTGCAAGTGGTGACTCTGGCAGTGGGCTCGGAGACGCAAGAGAG
TTACTCAGGGAGGAGACTGGCGCGGCCCTAAAACCTAGGCTTCCCGCACCACCCCCCGTC
ACAACACCACCTTTGGCTGGAGGAAGCAGCACAGAAGATTCCAGAAGTGAAGAAGTTGAG
GAGCAGACTACAGTGGCTTCGTTAGGAGCTCAGACACTTCCTGGATCAGATTCTGTCTCA
ACTTGGGACGGGAGTGTCCGGACTCCTGGTGGCAGAGTGAAAGAAGGAGGCCTGAAAGGT
CAGAAAGGGGAGCCAGGTGTTCCGGGTCCACCTGGCAGGGCAGGTCCTCCAGGATCACCA
TGCCTACCTGGTCCCCCGGGACTCCCATGCCCAGTGAGTCCCCTAGGTCCTGCAGGACCA
GCTTTGCAAACTGTTCCCGGACCTCAAGGTCCTCCAGGTCCTCCGGGAAGGGACGGCACA
CCTGGAAGAGACGGTGAGCCAGGCGACCCCGGTGAAGACGGAAAGCCAGGTGATACCGGG
CCACAAGGCTTTCCCGGTACTCCTGGGGACGTAGGTCCAAAAGGAGATAAGGGAGATCCT
GGTGTTGGAGAGAGAGGGCCTCCTGGACCACAAGGGCCTCCAGGTCCTCCAGGACCTTCC
TTCAGACACGACAAACTGACCTTCATTGACATGGAGGGATCTGGCTTCGGAGGCGATCTG
GAAGCCTTACGAGGTCCTAGAGGCTTCCCTGGTCCTCCAGGACCCCCCGGTGTCCCAGGC
TTACCCGGCGAGCCTGGCAGATTTGGGGTGAACAGCTCCGACGTACCAGGACCCGCCGGT
TTACCTGGTGTGCCTGGGCGCGAGGGTCCACCCGGGTTTCCTGGCCTTCCGGGACCTCCT
GGCCCTCCTGGAAGAGAGGGGCCCCCAGGAAGGACTGGGCAGAAAGGCAGCTTAGGTGAA
GCAGGCGCTCCTGGACATAAGGGGTCCAAAGGAGCTCCCGGTCCTGCTGGCGCTAGAGGA
GAGAGCGGACTTGCAGGCGCACCCGGACCTGCAGGACCACCAGGACCCCCTGGTCCACCT
GGACCACCAGGCCCCGGACTTCCCGCTGGTTTTGATGATATGGAAGGCTCCGGTGGACCT
TTTTGGTCAACAGCAAGAAGCGCTGATGGCCCACAGGGACCGCCTGGACTGCCGGGACTT
AAGGGGGATCCTGGCGTGCCTGGTCTGCCTGGGGCTAAGGGAGAAGTTGGAGCTGATGGA
GTCCCCGGTTTCCCCGGCTTACCTGGTAGAGAGGGTATTGCTGGACCCCAAGGGCCAAAG
GGAGATAGAGGCAGCAGAGGAGAAAAAGGAGATCCAGGGAAAGACGGAGTAGGACAGCCG
GGATTACCTGGCCCACCAGGACCCCCTGGACCTGTGGTCTATGTGTCGGAGCAGGACGGA
TCCGTTCTGAGCGTGCCTGGACCTGAAGGCCGGCCGGGTTTCGCAGGATTTCCAGGACCT
GCAGGACCTAAAGGCAATCTGGGCTCTAAAGGTGAACGAGGCTCTCCGGGACCAAAGGGT
GAGAAGGGAGAACCGGGCAGCATTTTCAGTCCAGATGGAGGTGCTCTGGGTCCTGCCCAG
AAAGGAGCCAAGGGAGAGCCAGGATTCCGAGGACCACCGGGTCCATATGGACGTCCGGGA
TACAAAGGTGAGATTGGATTTCCTGGACGGCCAGGTCGCCCCGGGATGAACGGATTGAAA
GGAGAGAAAGGTGAGCCTGGAGATGCAAGCCTTGGATTTGGAATGAGAGGAATGCCCGGC
CCCCCAGGACCTCCTGGGCCACCAGGTCCTCCAGGAACTCCTGTTTACGATAGCAATGTG
TTTGCTGAGTCCAGCCGACCAGGGCCTCCAGGATTGCCAGGGAATCAGGGACCTCCAGGA
CCTAAGGGCGCAAAAGGAGAAGTGGGCCCCCCCGGACCACCAGGGCAGTTTCCTTTTGAC
TTTCTTCAGTTAGAGGCTGAAATGAAGGGTGAAAAGGGAGATCGAGGTGATGCTGGACAA
AAAGGCGAAAGAGGGGAACCCGGTGGAGGCGGTTTCTTCGGTTCTAGTCTGCCCGGTCCT
CCCGGCCCTCCAGGTCCACGTGGGTACCCTGGGATTCCAGGTCCTAAAGGAGAGTCCATC
CGGGGACAACCAGGCCCACCTGGACCTCAGGGACCACCCGGCATTGGCTACGAAGGGCGC
CAAGGACCTCCCGGTCCCCCAGGTCCTCCAGGGCCCCCTTCATTCCCTGGACCTCACAGA
CAAACTATCAGCGTTCCCGGTCCTCCTGGTCCACCTGGTCCCCCTGGGCCTCCTGGAACC
ATGGGCGCTTCCTCAGGAGTTAGGCTCTGGGCTACAAGACAGGCTATGCTGGGCCAAGTG
CACGAGGTTCCAGAGGGCTGGCTTATTTTCGTGGCAGAGCAGGAGGAGCTTTACGTTCGA
GTGCAGAACGGTTTCAGGAAGGTACAGCTGGAGGCTAGGACACCACTCCCCAGAGGGACT
GACAATGAAGTGGCCGCTTTGCAGCCCCCCGTGGTGCAGTTACACGACAGCAATCCTTAC
CCAAGAAGGGAACACCCCCATCCCACCGCAAGACCTTGGAGGGCAGATGATATCCTTGCT
AGCCCTCCTCGTCTGCCAGAACCCCAGCCATACCCCGGAGCCCCACACCATAGCTCCTAC
GTGCATCTGCGTCCAGCTAGACCTACAAGTCCACCCGCTCACAGCCATAGAGACTTTCAG
CCGGTGCTCCACTTAGTTGCTCTCAATTCCCCACTGTCTGGCGGTATGAGAGGCATACGC
GGAGCCGACTTCCAGTGTTTCCAGCAGGCTAGAGCCGTGGGACTGGCAGGAACCTTTCGC
GCCTTCCTTTCTTCTAGACTTCAGGACCTGTATAGCATCGTTCGCCGTGCCGATCGCGCA
GCTGTGCCCATCGTCAATCTCAAGGACGAGTTGCTGTTTCCCAGTTGGGAGGCTCTTTTC
TCAGGATCAGAGGGTCCTTTAAAGCCCGGAGCTCGCATATTCTCTTTTGACGGTAAAGAC
GTCCTGAGGCATCCTACATGGCCCCAAAAGAGTGTGTGGCATGGCTCTGACCCCAACGGA
CGCAGACTGACAGAGAGCTACTGTGAGACATGGCGGACAGAGGCTCCCTCTGCCACTGGC
CAGGCTTCCTCTCTGTTAGGGGGCAGGCTCCTGGGGCAAAGCGCCGCTAGTTGCCATCAC
GCTTACATCGTGCTCTGCATTGAGAACAGCTTTATGACTGCTTCCAAG
Protein sequence predicted based on nucleotide sequence
MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADT TTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKG IRSFVQLWNDTVPTESLARAETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGA HTTEAGTLPAPTPSPPSLGRPWAPLTGPSVPPPSSERISEEVGLLQLLGDPPPQQVTQTD DPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVL LGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDC EEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEE GDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVE EQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSP CLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTG PQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDL EALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPP GPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPP GPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADG VPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDG SVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQ KGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPG PPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFD FLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESI RGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGT MGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGT DNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSY VHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFR AFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKD VLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHH AYIVLCIENSFMTASK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
