Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-004.0191
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821148
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4632nts
Open reading frame: 1 to 4632
ATGGAGGCTGCAACAACACTTCACCCAGGACCTCGCCCTGCTCTTCCCCTTGGTGGCCCT
GGCCCTTTGGGAGAATTCCTGCCTCCACCCGAGTGTCCGGTTTTCGAACCCAGCTGGGAA
GAGTTTGCGGACCCCTTCGCCTTCATCCACAAGATCAGGCCCATAGCCGAGCAGACTGGC
ATCTGTAAGGTGCGGCCGCCGCCGGATTGGCAGCCACCATTTGCATGTGATGTTGATAAA
CTGCATTTTACGCCACGGATCCAGAGACTGAATGAATTAGAGGCCCAAACTCGTGTAAAA
TTGAATTTCCTGGACCAGATTGCAAAGTACTGGGAGCTACAGGGAAGTACTCTCAAAATT
CCACATGTCGAGAGGAAGATCCTGGACTTATTTCAGCTTAATAAGTTAGTTGCAGAAGAA
GGTGGATTTGCCGTGGTTTGCAAGGATAGAAAATGGACCAAAATTGCTACCAAGATGGGG
TTTGCTCCTGGCAAAGCAGTGGGCTCACATATCAGAGGGCATTATGAACGAATTCTCAAC
CCCTACAACTTATTCCTGTCTGGCGACAGCCTAAGGTGTTTGCAGAAGCCAAACCTGACC
ACAGACACTAAGGACAAGGAGTACAAACCCCATGATATTCCCCAGAGGCAGTCAGTGCAG
CCTTCGGAAACGTGCCCCCCCGCCCGACGAGCAAAACGCATGAGAGCAGAGGCCATGAAT
ATTAAAATAGAACCCGAGGAGACAACGGAAGCCAGAACTCATAATCTGAGACGTAGAATG
GGTTGTCCAACTCCTAAATGTGAGAACGAGAAAGAAATGAAGAGTAGCATCAAGCAAGAA
CCTATTGAGAGGAAAGATTATATTGTAGAAAACGAGAAGGAAAAGCCCAAGAGTCGATCT
AAAAAAGCCACAAATGCTGTGGACCTGTATGTCTGTCTGTTATGTGGGAGTGGCAATGAT
GAAGACCGGCTACTGCTGTGTGATGGCTGTGATGACAGTTACCATACCTTTTGCTTGATC
CCACCTCTACATGATGTTCCCAAGGGCGACTGGAGGTGTCCTAAGTGTTTGGCTCAGGAA
TGTAGTAAGCCACAAGAAGCATTTGGCTTTGAACAAGCAGCTAGGGACTATACCCTCCGG
ACTTTTGGGGAAATGGCAGATGCGTTCAAATCTGATTACTTCAACATGCCAGTCCATATG
GTGCCCACAGAGCTTGTGGAGAAAGAATTTTGGAGACTGGTATCCACTATTGAGGAGGAT
GTTACAGTCGAATATGGCGCTGACATTGCCTCAAAGGAATTTGGCAGTGGATTTCCTGTC
CGAGATGGGAAAATCAAACTCTCACCTGAGGAAGAGGAGTATCTGGATAGTGGCTGGAAT
TTGAACAACATGCCAGTGATGGAGCAGTCTGTCCTTGCACATATTACTGCTGATATATGT
GGCATGAAACTTCCTTGGTTGTATGTGGGAATGTGCTTTTCTTCATTCTGTTGGCACATT
GAAGACCACTGGAGCTATTCAATTAACTACTTGCACTGGGGTGAGCCAAAGACCTGGTAT
GGAGTGCCAGGGTATGCTGCTGAGCAGCTGGAAAATGTAATGAAGAAACTAGCTCCAGAA
CTCTTTGTGTCACAGCCGGATCTCCTCCATCAGCTTGTGACCATCATGAACCCCAATACC
CTGATGACTCATGAAGTGCCTGTTTACCGAACTAATCAGTGTGCTGGGGAGTTTGTGATT
ACCTTCCCAAGAGCCTACCACAGTGGTTTTAACCAGGGTTTTAATTTTGCTGAGGCTGTT
AACTTCTGCACTGTTGATTGGCTGCCATTAGGCCGACAGTGTGTGGAGCATTATCGCTTG
CTTCATCGATATTGTGTGTTTTCCCACGATGAGATGATCTGCAAGATGGCCTCCAAGGCT
GATGTATTAGATGTTGTAGTGGCTTCTACTGTTCAGAAAGACATGGCCATTATGATTGAG
GATGAGAAAGCTTTAAGAGAGACTGTCCGTAAATTGGGAGTGATTGATTCGGAAAGAATG
GATTTTGAGCTATTGCCAGATGATGAACGGCAGTGTGTAAAATGCAAAACTACATGCTTC
ATGTCTGCCATCTCCTGTTCTTGTAAACCTGGCCTTCTGGTTTGCCTGCATCATGTAAAG
GAATTGTGTTCCTGTCCTCCTTACAAATATAAATTGCGGTATAGGTACACGCTGGATGAT
CTCTACCCTATGATGAATGCATTGAAGCTTAGAGCAGAATCTTACAACGAATGGGCCCTG
AATGTGAATGAAGCTTTGGAGGCAAAGATCAACAAGAAGAAAAGCCTTGTCAGCTTCAAG
GCTTTAATTGAAGAATCTGAAATGAAGAAATTCCCAGACAATGATCTTTTGCGACACCTT
CGCCTAGTCACACAGGATGCAGAGAAGTGTGCCTCTGTTGCGCAGCAGCTGCTTAATGGC
AAACGCCAAACTAGATATCGATCTGGCGGAGGGAAATCCCAAAATCAGTTGACAGTGAAT
GAGCTCCGGCAGTTTGTAACACAGCTGTATGCTCTGCCATGTGTCCTCAGTCAGACACCC
TTACTGAAGGATCTCTTGAATCGTGTAGAAGATTTTCAACAGCATAGCCAGAAACTACTC
TCCGAGGAAACGCCTAGTGCTGCGGAGCTGCAGGACTTGCTGGATGTCAGCTTTGAATTT
GATGTGGAACTTCCACAGCTTGCTGAGATGCGTATCCGTTTGGAACAAGCCCGTTGGCTT
GAAGAGGTGCAGCAAGCTTGCCTAGACCCCAGCTCCCTCACTCTCGATGATATGCGACGT
CTCATAGACCTAGGGGTAGGCCTGGCCCCCTACTCAGCAGTGGAGAAAGCTATGGCTCGG
CTGCAGGAACTGCTGACAGTGTCAGAGCACTGGGACGACAAAGCCAAGAGTCTCCTCAAG
GCCAGGCCACGGCATTCATTGAATAGCCTGGCTACGGCAGTAAAGGAAATCGAAGAGATC
CCTGCATATCTGCCCAATGGTGCGGCTCTGAAAGACTCAGTGCAGAGAGCCAGAGACTGG
CTTCAGGATGTAGAGGGCCTGCAGGCTGGAGGCCGGGTGCCAGTGTTAGACACCCTCATA
GAACTTGTTACACGGGGCCGATCGATCCCTGTACATCTGAATTCTTTGCCAAGGCTCGAA
ACCCTAGTAGCCGAGGTTCAGGCTTGGAAAGAATGTGCTGTTAATACATTCTTGACCGAG
AATTCTCCATATTCTCTCTTAGAGGTGCTGTGTCCCCGATGTGATATTGGCCTTTTGGGA
TTGAAAAGGAAGCAGAGAAAGTTAAAGGAGCCACTCCCAAATGGAAAGAAAAAAAGCACC
AAATTGGAGAGTCTGAGCGACCTCGAGAGAGCTTTGACTGAAAGCAAGGAGACTGCCTCA
GCTATGGCAACTCTTGGGGAAGCCCGCCTGAGGGAAATGGAAGCCTTGCAGTCCCTCAGA
CTCGCCAATGAAGGGAAATTGCTGTCGCCTCTTCAAGATGTGGATATAAAAATCTGCCTA
TGTCAGAAGGCCCCAGCTGCCCCTATGATTCAATGTGAACTCTGCAGGGATGCTTTCCAC
ACCAGTTGTGTGGCGGTACCCAGTATTTCACAGGGACTGCGAATCTGGCTTTGTCCCCAT
TGTCGGAGGTCAGAGAAACCTCCATTAGAGAAAATTCTCCCCCTGCTCGCCTCCCTCCAG
CGTATCCGAGTGCGCCTCCCTGAGGGAGATGCACTTCGATATATGATCGAAAGAACCGTG
AACTGGCAGCACAGGGCCCAGCAACTGTTGTCGTCAGGGAATCTGAAATTTGTCCAAGAT
CGAGTGGGATCAGGACTGTTATATAGCAGATGGCAAGCCTCAGCAGGACAGGTGTCAGAC
ACAAACAAGGTATCTCAACCTCCTGGCACAACATCTTTCTCTTTACCTGATGACTGGGAC
AACAGAACCTCATATCTGCACTCCCCCTTCTCCACTGGACGAAGTTGTATCCCCCTCCAT
GGTGTTAGTCCCGAAGTGAATGAACTGTTGATGGAAGCCCAGCTGCTCCAGGTATCCCTT
CCTGAAATTCAGGAACTTTACCAGACTTTACTTGCAAAGCCATCTCCTGCTCAGCAGACT
GACCGAAGTTCACCAGTCAGACCCAGCAGTGAGAAGAATGACTGTTGTCGGGGGAAGCGA
GATGGAATTAACAGTCTTGAGAGAAAACTCAAGAGACGCCTTGAAAGAGAGGGCCTTTCC
AGTGAGCGGTGGGAACGAGTTAAGAAAATGCGGACTCCCAAAAAGAAGAAAATCAAACTG
AGCCACCCTAAGGACATGAACAATTTCAAGTTGGAGAGAGAGCGTAGCTATGAATTAGTT
CGTTCTGCTGAAACCCATTCCCTGCCCTCAGACACATCCTATTCCGAACAGGAAGACTCT
GAGGATGAAGACGCTATCTGCCCAGCTGTGAGCTGCCTGCAGCCAGAAGGAGATGAGGTG
GACTGGGTCCAGTGTGATGGCAGCTGCAATCAGTGGTTTCATCAGGTCTGTGTTGGTGTC
TCTCCAGAGATGGCAGAGAAAGAAGACTACATCTGTGTGCGCTGTACTGTGAAGGACGCC
CCAAGCCGAAAG
Protein sequence predicted based on nucleotide sequence
MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTG ICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKI PHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILN PYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMN IKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRS KKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQE CSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEED VTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADIC GMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPE LFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAV NFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIE DEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVK ELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFK ALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVN ELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEF DVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMAR LQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDW LQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTE NSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETAS AMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFH TSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTV NWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWD NRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQT DRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKL SHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEV DWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
