Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-011.0118
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821290
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5646nts
Open reading frame: 1 to 5646
ATGGTCGGCCGCGGAGTCCCTCTGTGCGCAGCGCAGCCCGCGGTTGCCGAAGGCGGCCCG
GCCCGCGAACCGCCGCCTTTATTAGAAGTGTCACCCCGCAAGAGGCTACCCGCCGGGCCA
GATCAGGATCCATGTGGCTCACGCCCTGCACCCGAAGGAGCAGGGGCCGGTCCAGAGCAA
GGCCACTCGGCCGGCGGAGGCGGCTGGTGCAGACACTGCCACACGAAGTTAGTAGAGCTT
AAGAGACAGGCGTGGAAGCTGGTTAGCGGGCCCGGAACTACCCTCCGTGATCCTTGCCTC
TCTGCCTTGCTTCTAGACAAGCTACCAGCACCTGGCGCCCTTCCTGCCTGTCGCCCAGAA
GCAGAACGTCGCTGTGACGTCTGCGCCACACATCTGCAACAGCTCACACGGGAGGCCATG
CACCTTCTACAGGCCCCTGCCAGCCATGAGGACCTTGACGCCCCCCATGGAGGCCCCAGT
CTAGCTCCCCCGAGCACCACGACAAGCTCGAGGGACACGCCGGGGCCAGCGGGTCCAGCA
GGGAGACAACCAGGACGCGCTGGACCAGATAGGACCAAGGGGCTGGCCTGGTCGCCCGGG
CCCTCAGTCCAGGTTTCTGTAGCTCCCGCGGGTCTTGGAGGGGCTCTGAGCACGGTTACC
ATCCAGGCCCAGCAATGCCTGGAGGGCATGTGGAGTGTTTCACGTGTCAACAGCTTCCTC
CCGCCTGCATGCCTAGCCGAAGCAGCGGTTGCGGCCGTGGCGGTAGCAGACACGGTCCGA
GAATGCCCCCCAGTGGCAGGCCCTGATGGCTTGTCAAAAGCCTGGGGCAGAGGTGGAGTC
TGTACATCAGCCCTCGTCACCCCCACACCGGGATCGGTGGGGGGCTCCACAGGACCCTCA
GCGGCAGCCTCCTTCTTCATTAGGGCTATGCAGAAGCTCAGCTTGGCTTCCAAGAGGAAA
AAACCCCACCCGCCACCACCGCCAGCAACCCGCGGAACCTCCACCTACCCTACCGACTTC
AGCGGGGTCCTGCAGTTGTGGCCGCCTCCGGCGCCCCCTTGCTTGTTGCGAGCCGCTTCT
AAGACCAAGGACAACCCTGGCTCTATCGGGAAGGTGAAGGTTATGCTGCGCATCTGGCCG
GCACAGGGGGCCCAACGCTCTGCCGAGGCTATGTCCTTCCTAAAGGTTGACCCTCGGAAG
AAGCAGGTAATCCTCTACGATCCCGCAGCCGGTCCCCCAGGCAGCGCGGGCCCACGACGA
GCCGCGACTGCGGCAGTTCCCAAGATGTTTGCTTTCGATGCCGTCTTTCCCCAAGACTCC
GAGCAGGCAGAAGTCTGCTCAGGGACCGTGGCCGACGTGCTCCAGTCTGTGGTATCAGGT
GCGGATGGCTGCATTTTTTCGTTTGGACACATGAGCCTGGGAAAGTCTTATACCATGATA
GGGAAGGATAGCTCACCGCAGAGCCTAGGCATCGTGCCATGCGCCATCAGTTGGCTCTTC
AGGTTGATCGAGGAGCGCCGAGAGAGGACGGGCACTCGCTTCTCCGTCCGGGTATCAGCC
GTGGAGGTGTGCGGGCGCGACCAGAGCCTGCGCGACCTGTTAGCCGAGGTAGCCCCCGGA
AGCCTCCAGGATACCCAGTCTCCGGGAGTGTACCTCCGAGAGGATCCCGTATGCGGGGCG
CAACTCCAGAATCAGAGCGAGTTGCGGGCACCAACGGCCGAGAAGGCGGCTTTCTACCTG
GATGCGGCCCTTGCGGCACGTAGTACCAGCCGAGCCGGCTGTGGAGAAGACGCCCGAAGA
TCATCCCACATGTTGTTCACGCTGCATGTTTACCAGTACCGCATGGAGAAGTGCGGCCGG
GGCGGAATGTCCGGAGGTCGCTCCCGCTTACACCTCATCGACCTTGGCAGCTGTGAGGCC
GCAGCTGGCAGAGCTGGGGAGGCTGCTGGGGGTCCACTGTGTCTGTCTCTGTCGGCCCTG
GGCAGCGTCATCTTGGCCCTAGTTAATGGAGCTAAGCATGTACCGTATCGGGATCATAGG
CTCACAATGCTCTTGCGTGAATCCCTGGCCACGGCTGGCTGCCGTACCACAATGATCGCC
CACGTGTCGGATGCGCCAGCACAGCACGCAGAGACATTAAGCACCGTGCAGCTCGCCGCC
CGAATCCATCGCCTGCGAAGGAAGAAGGCCAAGTACGCTTCAAGCTCCTCTGGCGGGGAG
AGCTCTTGTGAGGAAGGCCGCGCCCGTCGGCCCCCGCACTTACGGCCGTTCCACCCTCGT
ACTGTGGCACTGGACCCCGATCGCACCCCCCCCTGCTTGCCCGGTGATCCAGATTACTCC
TCTAGCAGCGAGCAGTCCTGTGATACGGTCATATACGTGGGGCCCGGTGGTGCGGCGTTG
TCAGACAGGGAGCTAACCGACAACGAAGGTCCGCCTGACTTCGTGCCCATCATCCCTGCC
CTGAGCCGACACCGGCCATCCAAAGGTCCCCGGGACGCAGATCACTTCCGATGCAGCACC
TTTGCGGAGCTGCAGGAGCGGCTAGAATGCATGGACGGCAATGAAGGTCCCTCAGGAGGT
CCAGGTGGCACCGACGGAGCTCAAGCTAGTCCCGCCCGAGGGGGTCGAAAGCCCTCGCCA
CCAGAAGCTGCATCCCCCCGAAAGGCCGTAGGCACCCCGATGGCCGCCAGTACCCCTAGA
GGCAGTTCAGGGCCAGACACCCACCAGGGTACCCCTGAACCCTGTAAGGCCATTGTCTGG
GGTGACCAGAGGGAGGATAGCAGCGCGTGGCCTGAGCTGCTGGTACCGGAAAAGGCTGCA
GTGAGTGGAGGCAGGAGGCCACTGCCCAGCCCGGCTCCGCCGCCACCTCAGTTACTGGAA
GCCTGCAGAGCGCCAGAAGAGCCCGGGGGAGGGGGCACTGACGGAGTGGCACGGACCCCT
CCTGTGGGCATGTCTGGTCAGGTGGCTGGGTCACCGATGCTTCCTGGGGCCACCTGTCCC
CGGTTGGCTGCTGGCAGTCGCTGTCCGGAACGAGGCCTGCTCACCACAACAGTGACCCTT
CAACGGCCAGTGGAACTCAACGGTGAGGACGAGCTGGTGTTCACGGTGGTTGAAGAGCTG
TCCCTGGGGGCGCTTGCCGGTGCTGGGAGGCCCACAAGCCTGGCTAGCTTCGACAGTGAC
TGCTCCCTTCGGGCCCTGGCCTCGGGTTCCCGGCCAGTCAGCATCATTAGCAGCATCAAT
GATGAGTTTGACGCCTACACCTCCCAGGCACCTGAAGGGGGACCCCTGGAAGGGGCAGCC
TGGGCCGGCAGCAGCCACGGATCCTCCATCAGCTCCTGGCTCAGCGAGGTCAGCGTCTGC
ACTGCCGACAGCCGTGACCCCACGCCTCAACCCCGCTTCAGCCCCGATAGTCTAGCAGGA
TTAGACCCTGGAGGTCCTCCAGCTCTAGATGGTTCCCTAGGGGATGGAAGCTCTGGCTTC
CTAGGTCCAGACAGACCTGACAGTCCTGGGCCTACCTGGGGTCCTTGTCCTGGGGAAGTA
GCTGCCGTTGCCCCATCCCGACCCGGCAGGGAACCCCAGGCCGGGCCCAGTCGGTGGGCT
TCGGCAGCTCAAACAATTCACTCCTCACTCCCCCGTAAACCGAGGACTGCAAGTGCAACC
ACCCGTGTGGGCTGTGCTCGACTTGGCCAGAGTCCACCTGGTCGTGGAGGCCTGTTTGAG
GACCCATGGCTACTCAGAGTAGGAGAGTGCGATACTCAGGCAGCATCTGCTGGCCGAGCC
CCCAGCCCTACTCTTGGCTCCCCCCGGCTACCTGAGGCTCAGGTGATGCTAGCCTGTGCA
CAAAGAGTGGTTGACGGATGTGAGGTTGCTGCCAGGGCTGCCCGCAGGCCCGAGGCTGTC
GCTCGGATCCCACCGCTGCGGAGGGGTGCTACCACGCTGGGTGTTACAACGCCAGCTGTA
TCTTGGGGAGATGCTCCCACGGAGGTTGTAGCTTGCAGTGGGAGCTTAAAGGCTTCCCCA
ACCAGCAAGAAGGGTCTGGCTCCAAAGGCGGGCTTTCTCCCGCGACCCAGTGGGGCAGCA
CCCCCGGCGCCACCCACGCGGAAATCTTCGCTGGAGCAACGGTCCAGTCCGGCCTCGGCT
CCTCCCCATGCAGTGAACCCGGCGCGCGTTGGCGCTGCTGCTGTCTTACGAGGGGAAGAG
GAACCCAGACCCAGCAGTCGGGCAGACCACTCTGTCCCCAGGGCTACGTCCAGCCTTAAA
GCACGGGCATCTAAGGTAGAAGCAGCACACCGTCTGGCTGGACACGCGTCTCTGGAGAGA
TACGAAGGCCTTGCGCACTCGAGCAGCAAGGGACGGGAAGCCCCTGGGCGGCCTCCCCGT
GCTGTACCCAAACTGGGTGTACCACCCTCCAGCCCCACACACGGGCCAGCTCCAGCCTGT
AGGAGCGGAGCGGCCAAGGCTGTTGGGGCACCCAAGCCACCAGTTGGTGGGGGTAAGGGC
CGTGGACTAGTGGCTGGTGGTAGTCGGGCTTTAGGGCCTTCGGTTAAACTGTCTACGGCT
TCTGTGACGGGCAGGAGCCCAGGCGGTCCCGTCGCTGGTCCCAGAGCAGCTCCACGAGCT
GGGCCGTCCGTCGGGGCGAAGGCTGGTCGGGGTACCGTCATGGGAACTAAGCAGGCGCTC
CGGGCTGCTCATTCTCGTGTACATGAGCTTTCAGCCTCCGGAGCCCCGGGCCGAGGTGGC
TCATCGTGGGGCTCAGCTGACTCAGACAGTGGCCATGACAGCGGCGTGAACGTGGGAGAA
GAGCGGCCCCCTACGGGCCCCGCCTTGCCTTCCCCATACAGCAAGGTGACGGCACCACGG
CGGCCCCAAAGGTACTCCAGCGGACATGGCAGCGACAACAGCAGCGTGCTGAGTGGTGAA
TTGCCGCCCGCCATGGGCAGAACCGCCCTTTTCCACCACAGCGGAGGCAGCAGTGGCTAT
GAGAGTCTGCGGCGAGACAGCGAAGCCACCGGAAGCGCCTCCTCCGCCCCCGACTCCATG
AGCGAGAGCGGGGCTGCCTCTCCAGGCGCCCGAACCCGCAGTCTCAAGTCACCCAAGAAG
AGGGCCACAGGTCTGCAGCGGCGGCGCCTGATTCCCGCCCCACTCCCCGATACAACTGCC
CTGGGACGTAAACCCTCGCTCCCCGGTCAGTGGGTGGACCTGCCCCCGCCCCTGGCTGGC
TCCCTGAAGGAGCCGTTCGAGATCAAAGTGTACGAGATCGATGACGTGGAGCGCTTACAG
CGGCCTCGACCCACCCCGCGAGAGGCCCCCACCCAGGGTTTGGCGTGCGTTAGTACAAGG
CTGCGGCTGGCGGAGCGCAGGCAACAACGGCTGCGAGAGGTGCAGGCCAAGCACAAGCAC
TTATGTGAGGAGCTAGCCGAGACACAGGGCCGTCTGATGCTGGAACCTGGCCGCTGGCTA
GAGCAATTTGAAGTAGACCCGGAACTGGAGCCCGAGTCGGCTGAATACCTTGCAGCTCTG
GAGCGGGCTACAGCCGCCCTGGAGCAGTGCGTGAACCTGTGCAAGGCGCACGTCATGATG
GTCACTTGTTTCGACATCAGCGTTGCGGCAAGTGCTGCCATCCCGGGTCCGCAGGAGGTG
GACGTT
Protein sequence predicted based on nucleotide sequence
MVGRGVPLCAAQPAVAEGGPAREPPPLLEVSPRKRLPAGPDQDPCGSRPAPEGAGAGPEQ GHSAGGGGWCRHCHTKLVELKRQAWKLVSGPGTTLRDPCLSALLLDKLPAPGALPACRPE AERRCDVCATHLQQLTREAMHLLQAPASHEDLDAPHGGPSLAPPSTTTSSRDTPGPAGPA GRQPGRAGPDRTKGLAWSPGPSVQVSVAPAGLGGALSTVTIQAQQCLEGMWSVSRVNSFL PPACLAEAAVAAVAVADTVRECPPVAGPDGLSKAWGRGGVCTSALVTPTPGSVGGSTGPS AAASFFIRAMQKLSLASKRKKPHPPPPPATRGTSTYPTDFSGVLQLWPPPAPPCLLRAAS KTKDNPGSIGKVKVMLRIWPAQGAQRSAEAMSFLKVDPRKKQVILYDPAAGPPGSAGPRR AATAAVPKMFAFDAVFPQDSEQAEVCSGTVADVLQSVVSGADGCIFSFGHMSLGKSYTMI GKDSSPQSLGIVPCAISWLFRLIEERRERTGTRFSVRVSAVEVCGRDQSLRDLLAEVAPG SLQDTQSPGVYLREDPVCGAQLQNQSELRAPTAEKAAFYLDAALAARSTSRAGCGEDARR SSHMLFTLHVYQYRMEKCGRGGMSGGRSRLHLIDLGSCEAAAGRAGEAAGGPLCLSLSAL GSVILALVNGAKHVPYRDHRLTMLLRESLATAGCRTTMIAHVSDAPAQHAETLSTVQLAA RIHRLRRKKAKYASSSSGGESSCEEGRARRPPHLRPFHPRTVALDPDRTPPCLPGDPDYS SSSEQSCDTVIYVGPGGAALSDRELTDNEGPPDFVPIIPALSRHRPSKGPRDADHFRCST FAELQERLECMDGNEGPSGGPGGTDGAQASPARGGRKPSPPEAASPRKAVGTPMAASTPR GSSGPDTHQGTPEPCKAIVWGDQREDSSAWPELLVPEKAAVSGGRRPLPSPAPPPPQLLE ACRAPEEPGGGGTDGVARTPPVGMSGQVAGSPMLPGATCPRLAAGSRCPERGLLTTTVTL QRPVELNGEDELVFTVVEELSLGALAGAGRPTSLASFDSDCSLRALASGSRPVSIISSIN DEFDAYTSQAPEGGPLEGAAWAGSSHGSSISSWLSEVSVCTADSRDPTPQPRFSPDSLAG LDPGGPPALDGSLGDGSSGFLGPDRPDSPGPTWGPCPGEVAAVAPSRPGREPQAGPSRWA SAAQTIHSSLPRKPRTASATTRVGCARLGQSPPGRGGLFEDPWLLRVGECDTQAASAGRA PSPTLGSPRLPEAQVMLACAQRVVDGCEVAARAARRPEAVARIPPLRRGATTLGVTTPAV SWGDAPTEVVACSGSLKASPTSKKGLAPKAGFLPRPSGAAPPAPPTRKSSLEQRSSPASA PPHAVNPARVGAAAVLRGEEEPRPSSRADHSVPRATSSLKARASKVEAAHRLAGHASLER YEGLAHSSSKGREAPGRPPRAVPKLGVPPSSPTHGPAPACRSGAAKAVGAPKPPVGGGKG RGLVAGGSRALGPSVKLSTASVTGRSPGGPVAGPRAAPRAGPSVGAKAGRGTVMGTKQAL RAAHSRVHELSASGAPGRGGSSWGSADSDSGHDSGVNVGEERPPTGPALPSPYSKVTAPR RPQRYSSGHGSDNSSVLSGELPPAMGRTALFHHSGGSSGYESLRRDSEATGSASSAPDSM SESGAASPGARTRSLKSPKKRATGLQRRRLIPAPLPDTTALGRKPSLPGQWVDLPPPLAG SLKEPFEIKVYEIDDVERLQRPRPTPREAPTQGLACVSTRLRLAERRQQRLREVQAKHKH LCEELAETQGRLMLEPGRWLEQFEVDPELEPESAEYLAALERATAALEQCVNLCKAHVMM VTCFDISVAASAAIPGPQEVDV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
