ZNF516 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821661
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3489nts
Open reading frame: 1 to 3489

ATGGATCGCAACAGAGAGGCCGAGATGGAGCTGAGGCGAGGACCCAGTCCCACCAGGGCC
GGCCGAGGACACGAGGTGGATGGGGACAAGGCTACCTGCCATACCTGCTGCATCTGTGGC
AAAAGCTTCCCCTTCCAGAGCTCGCTCTCGCAGCACATGCGCAAGCACACGGGCGAGAAG
CCCTACAAGTGTCCCTACTGCGACCACCGGGCTTCCCAGAAGGGCAACCTGAAGATTCAC
ATCCGGAGCCACCGCACGGGGACTCTCATTCAGGGACACGAACCAGAGGCGGGCGAGGCG
CCGCTGGGTGAGATGCGAGCATCTGAGGGCCTGGACGCATGCGCCAGCCCCACCAAGAGC
GCTTCGGCTTGCAACCGGCTGTTGAACGGGGCCTCGCAGGCTGACGGCGCCAGGGTCCTG
AATGGGGCCTCTCAGGCCGACAGCGGCAGAGTACTTCTCCGGAGCAGCAAGAAGGGGGCA
GAGGGGTCTGCATGCGCTCCGGGGGAGGCCAAGGCAGCGGTCCAGTGCTCCTTCTGCAAG
AGCCAGTTCGAGCGTAAGAAGGACCTGGAGCTGCACGTCCACCAGGCGCACAAGCCGTTC
AAGTGCAGGCTCTGCAGCTACGCGACGTTGCGGGAGGAGTCGCTCCTGAGTCACATCGAG
AGGGACCACATCACCGCGCAGGGGCCCGGCAGCGGCGAAGCCTGCGTGGAGAACGGAAAG
CCCGAGCTGAGCCCCGGAGAGTTTCCGTGTGAGGTGTGTGGCCAGGCCTTCAGCCAGACT
TGGTTCCTGAAGGCTCACATGAAGAAGCACCGGGGATCCTTCGACCACGGCTGCCACATC
TGCGGCCGTCGGTTCAAGGAGCCCTGGTTCCTCAAGAACCACATGAAGGCGCACGGCCCC
AAGACGGGGAGCAAGAACAGGCCCAAGAGTGAGCTCGACCCCATCGCCACCATCAACAAC
GTGGTCCAGGAGGAGGTGATCGTCGCTGGCCTGAGCCTTTACGAGGTCTGCGCCAAGTGC
GGGAACCTCTTTACAAACCTGGACAGCCTGAACGCCCACAATGCCATCCACCGAAGAGTC
GAGGCCAGCCGCACGCGCGCCCCGGCTGAGGAAGGGGCGGAAGGGCCCTCGGACACAAAG
CAGTTCTTCCTCCAGTGCCTCAACCTGAGGCCATCAGCGGCCGGCGACTCGTGCCCTGGC
ACACAGGCCGGAAGGCGGGTTGCTGAGCTGGACCCGGTCAACAGCTACCAGGCTTGGCAA
CTGGCCACGAGGGGTAAAGTGGCCGAGCCTGCCGAGTACCTCAAGTACGGGGCCTGGGAC
GAGGCGCTGGCCGGGGACGTGGCCTTCGACAAGGACAGGCGCGAGTACGTGCTGGTGAGC
CAGGAGAAGCGCAAGCGTGAGCAGGATGCACCAGCCGCGCAAGGGCCTCCGAGGAAACGC
GCGAGCGGTCCTGGAGATCCAGCTCCTGCAGGCCATTTAGATCCCCGATCGGCAGCGCGC
CCCAACCGCAGGGCCGCAGCCACCACAGGTCAGGGCAAGTCCTCCGAGTGCTTCGAGTGC
GGCAAGATCTTCCGCACCTATCATCAGATGGTGCTGCACTCACGCGTGCATCGACGCGCT
CGCCGTGAGAGGGACTCTGACGGGGACAGGGCTGCGCGGGCTCGATGCGGATCACTCAGT
GAGGGTGACTCGGCCTCCCAGCCCAGCAGTCCTGGCTCCGCCTGTGCAGCTGCTGACTCC
CCGGGATCAGGCCTCGCCGACGAAGCTGCCGAAGACAGTGGTGAAGAGGGCGCCCCTGAA
CCTGCACCAGGGGGACAGCCGCGACGCTGCTGCTTTTCCGAAGAGGTGACTTCGACCGAG
CTCTCCAGTGGCGACCAGAGTCACAAGATGGGAGATAACGCCTCGGAAAGAGACACCGGC
GAGTCCAAGGCAGGGATCGCAGCTTCTGTGTCCATACTTGAAAACAGTAGCAGAGAGACT
TCTAGAAGGCAAGAGCAGCATAGATTTTCTATGGACTTGAAGATGCCAGCATTTCACCCC
AAGCAGGAGGTGCCCGTCCCTGGTGATGGTGTGGAGTTCCCTTCCAGTACGGGAGCGGAG
GGCCAGACGGGTCACCCTGCAGAAAAGCTGTCCGATTTGCACAACAAGGAACACTCTGGG
GGAGGGAAGCGGGCGCTAGCCCCAGACCTCATGCCGCTAGATTTAAGTGCGAGGTCGACG
CGGGATGACCCCAGCAATAAAGAGACGGCCTCCTCCCTTCAGGCGGCTTTAGTCGTTCAC
CCGTGTCCTTACTGCAGCCACAAGACCTACTACCCCGAGGTCCTCTGGATGCACAAACGC
ATCTGGCACCGCGTCAGCTGCAACTCCGTGGCTCCCCCGTGGATTCAGCCCAATGGTTAC
AAAAGCATCAGAAGCAATCTGGTTTTCCTTTCCCGGAGCGGACGCACGGGACCCCCGCCT
GCCCTCGGTGGCAAAGAATGCCAGCCTTTGCTCCTTGCTCGGTTCACCCGCACTCAGGTG
CCAGGCGGGATGCCGGGGTCCAAAAGTGGCTCTTCTCCCCTGGGAGTGGTCACAAAAGCC
GCTAGCATGCCTAAGAATAAGGAGAGCCATTCCGGAGGTCCCTGCGCTCTGTGGGCGCCC
GGCCCTGACGGGTATCGACAGACCAAACCTTGTCACGGCCAGGAGCCACATGGCGCTGCT
ACACAGGGGCCCTTGGCTAAGCCCAGGCAGGAGGCTAGTTCCAAACCGGTGCCTGCCCCG
GGTGGCGGGGGATTCAGCAGGAGTGCCACCCCTACGCCCACCGTCATCGCCAGAGCTGGC
GCGCAACCCTCGGCCAATAGTAAGCCTGTGGAGAAGTTTGGGGTCCCCCCAGCGGGGGCT
GGCTTTGCCCCCACAAATAAGCACAGTGCCCCGGACTCCCTGAAAGCCAAATTCAGTGCT
CAGCCTCAGGGTCCACCCCCTGCAAAGGGCGAAGGGGGCGCTCCTCCTCTACCCCCCCGT
GAGCCCCCCTCGAAGGCAGCCCAGGAACTTAGGACTCTGGCCACCTGTGCTGCTGGGTCT
AGAGGCGATGCGGCCTTGCAGGCCCAGCCTGGCGTGGCTGGGGCGCCTCCCGTCCTACAC
TCTATCAAACAGGAGCCGGTGGCTGAGGGGCATGAGAAGCGCCTGGACATCCTCAACATC
TTTAAGACGTACATTCCAAAGGACTTTGCGACACTCTACCAGGGATGGGGTGTCAGCGGA
CCTGGGTTGGAGCACAGGGGTACACTCCGGACGCAGGCCCGGCCTGGAGAGTTCGTCTGC
ATCGAATGCGGAAAGAGCTTCCACCAGCCCGGCCACCTCAGGGCCCACATGAGGGCACAC
TCAGTGGTGTTTGAGTCCGATGGGCCTCGGGGTTCTGAAGTTCATACCACCTCCGCAGAC
GCCCCCAAACAAGGGAGAGATCATTCTAACACAGGTACCGTCCAGACAGTGCCTCTGAGA
AAGGGAACC

Protein sequence predicted based on nucleotide sequence

MDRNREAEMELRRGPSPTRAGRGHEVDGDKATCHTCCICGKSFPFQSSLSQHMRKHTGEK
PYKCPYCDHRASQKGNLKIHIRSHRTGTLIQGHEPEAGEAPLGEMRASEGLDACASPTKS
ASACNRLLNGASQADGARVLNGASQADSGRVLLRSSKKGAEGSACAPGEAKAAVQCSFCK
SQFERKKDLELHVHQAHKPFKCRLCSYATLREESLLSHIERDHITAQGPGSGEACVENGK
PELSPGEFPCEVCGQAFSQTWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKAHGP
KTGSKNRPKSELDPIATINNVVQEEVIVAGLSLYEVCAKCGNLFTNLDSLNAHNAIHRRV
EASRTRAPAEEGAEGPSDTKQFFLQCLNLRPSAAGDSCPGTQAGRRVAELDPVNSYQAWQ
LATRGKVAEPAEYLKYGAWDEALAGDVAFDKDRREYVLVSQEKRKREQDAPAAQGPPRKR
ASGPGDPAPAGHLDPRSAARPNRRAAATTGQGKSSECFECGKIFRTYHQMVLHSRVHRRA
RRERDSDGDRAARARCGSLSEGDSASQPSSPGSACAAADSPGSGLADEAAEDSGEEGAPE
PAPGGQPRRCCFSEEVTSTELSSGDQSHKMGDNASERDTGESKAGIAASVSILENSSRET
SRRQEQHRFSMDLKMPAFHPKQEVPVPGDGVEFPSSTGAEGQTGHPAEKLSDLHNKEHSG
GGKRALAPDLMPLDLSARSTRDDPSNKETASSLQAALVVHPCPYCSHKTYYPEVLWMHKR
IWHRVSCNSVAPPWIQPNGYKSIRSNLVFLSRSGRTGPPPALGGKECQPLLLARFTRTQV
PGGMPGSKSGSSPLGVVTKAASMPKNKESHSGGPCALWAPGPDGYRQTKPCHGQEPHGAA
TQGPLAKPRQEASSKPVPAPGGGGFSRSATPTPTVIARAGAQPSANSKPVEKFGVPPAGA
GFAPTNKHSAPDSLKAKFSAQPQGPPPAKGEGGAPPLPPREPPSKAAQELRTLATCAAGS
RGDAALQAQPGVAGAPPVLHSIKQEPVAEGHEKRLDILNIFKTYIPKDFATLYQGWGVSG
PGLEHRGTLRTQARPGEFVCIECGKSFHQPGHLRAHMRAHSVVFESDGPRGSEVHTTSAD
APKQGRDHSNTGTVQTVPLRKGT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable