Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
"insert_ID 57457,DNASU_Clone_ID HsCD00082585"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00824555
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2997nts
Open reading frame: 1 to 2997
ATGGGTTGGAAGCCCAGCGAGGCTAGAGGCCAGTCCCAAAGTTTCCAGGCATCAGGGCTG
CAGCCCAGGAGCCTCAAGGCGGCCCGGCGGGCGACTGGACGGCCGGACAGGTCCCGAGCA
GCCCCGCCCAACATGGACCCAGACCCCCAGGCGGGCGTGCAGGTGGGCATGCGGGTGGTG
CGCGGCGTGGACTGGAAGTGGGGCCAGCAGGACGGCGGCGAGGGCGGCGTGGGCACGGTG
GTGGAGCTTGGCCGCCACGGCAGCCCCTCGACACCCGACCGCACAGTGGTCGTGCAGTGG
GACCAGGGCACGCGCACCAACTACCGCGCCGGCTACCAGGGCGCGCACGACCTGCTGCTG
TACGACAACGCCCAGATCGGCGTCCGGCACCCCAACATCATCTGTGACTGCTGCAAGAAG
CACGGGCTGCGGGGGATGCGCTGGAAGTGCCGTGTGTGCCTGGACTACGACCTCTGCACG
CAGTGCTACATGCACAACAAGCATGAGCTCGCCCACGCCTTCGACCGCTACGAGACCGCT
CACTCGCGCCCTGTCACACTGAGTCCCCGCCAGGGCCTCCCGAGGATCCCACTAAGGGGC
ATCTTCCAGGGAGCGAAGGTGGTGCGAGGCCCCGACTGGGAGTGGGGCTCACAGGATGGA
GGGGAAGGGAAACCGGGCCGTGTGGTGGACATCCGTGGCTGGGATGTGGAGACAGGCCGG
AGTGTGGCCAGCGTGACGTGGGCTGATGGTACCACCAATGTGTACCGTGTGGGCTACAAG
GGCAAGGTGGACCTCAAGTGTGTGGGCGAGGCAGCGGGCGGCTTCTACTACAAGGACCAC
CTCCCAAGGCTCGGCAAGCCGGCGGAGCTGCAGCGCAGGGTGAGTGCTGACAGCCAGCCC
TTCCAGCACGGGGACAAGGTCAAGTGTCTGCTGGACACTGATGTCCTGCGGGAGATGCAG
GAAGGCCACGGCGGCTGGAACCCCAGGATGGCGGAGTTTATCGGACAGACGGGCACCGTG
CATCGTATCACGGACCGCGGGGACGTGCGCGTGCAGTTCAACCACGAGACGCGCTGGACC
TTCCACCCCGGGGCGCTCACCAAGCACCACTCCTTCTGGGTGGGCGACGTGGTCCGGGTC
ATCGGCGACCTTGACACAGTGAAGCGGCTGCAGGCTGGGCATGGCGAGTGGACGGACGAC
ATGGCCCCTGCCCTGGGCCGCGTCGGGAAGGTGGTGAAAGTGTTTGGAGACGGGAACCTG
CGTGTAGCAGTCGCTGGTCAGCGGTGGACCTTCAGCCCCTCCTGCCTGGTGGCCTACCGG
CCCGAGGAGGATGCCAACCTGGACGTGGCCGAGCGCGCCCGGGAGAACAAAAGCTCACTG
AGCGTGGCCCTGGACAAGCTTCGGGCCCAGAAGAGTGACCCAGAGCACCCGGGAAGGCTG
GTGGTGGAGGTGGCGCTGGGTAACGCAGCCCGGGCTCTGGGCCTGCTGCGGAGGCGCCCA
GAGCAGGTGGACACCAAGAACCAAGGCAGGACCGCTCTGCAAGTGGCTGCCTACCTGGGC
CAGGTGGAGTTGATACGGCTGCTGCTACAAGCCAGGGCGGGCGTGGACCTGCCGGACGAC
GAGGGCAACACGGCACTGCACTACGCGGCCCTGGGGAACCAGCCCGAGGCCACCAGGGTG
CTCCTGAGTGCTGGGTGCCGGGCGGACGCCATCAACAGCACCCAGAGCACAGCACTGCAC
GTGGCCGTGCAGAGGGGCTTCCTGGAGGTGGTGCGGGCCCTGTGTGAGCGCGGCTGTGAC
GTCAACCTGCCCGACGCCCACTCGGACACGCCCCTGCACTCCGCCATCTCGGCGGGCACT
GGAGCCGGCGGCATTGTCGAGGTCCTCACGGAGGTGCCAAACATCGATGTTACCGCCACC
AACAGCCAGGGTTTCACCCTGCTGCACCATGCCTCCCTCAAGGGTCACGCGCTAGCTGTG
AGAAAGATTCTGGCTCGGGCGCGGCAGCTGGTGGACGCCAAGAAGGAGGACGGCTTCACG
GCGCTGCATCTGGCTGCCCTCAACAACCACCGCGAGGTGGCCCAGATCCTCATCCGGGAG
GGCCGCTGTGACGTGAACGTGCGCAACCGGAAGCTGCAGTCCCCGCTGCATCTCGCCGTG
CAACAGGCCCACGTGGGGCTGGTGCCGCTACTGGTGGACGCTGGGTGCAGTGTCAACGCC
GAGGACGAGGAGGGGGACACAGCCCTGCACGTGGCGCTGCAGCGTCATCAGCTGCTGCCC
CTGGTGGCTGATGGGGCCGGGGGGGACCCAGGGCCCTTGCAGCTGCTGTCCAGGCTACAG
GCCTCGGGCCTCCCCGGCAGCGCGGAGCTGACGGTGGGCGCGGCGGTCGCCTGCTTCCTG
GCGCTGGAGGGCGCCGACGTGAGCTACACCAACCACCGCGGTCGGAGCCCGCTGGACCTG
GCCGCCGAGGGTCGCGTGCTCAAGGCCCTTCAGGGCTGCGCCCAGCGCTTCCGGGAGCGG
CAGGCGGGCGGGGGCGCGGCCCCGGGCCCCAGGCAAACGCTCGGGACCCCCAACACCGTG
ACGAACCTGCACGTGGGCGCCGCGCCGGGGCCCGAGGCCGCTGAGTGCCTGGTGTGCTCC
GAGCTGGCGCTGCTGGTGCTGTTCTCGCCGTGCCAGCACCGCACCGTGTGTGAGGAGTGC
GCGCGCAGGATGAAGAAGTGCATCAGGTGCCAGGTGGTCGTCAGCAAGAAACTGCGCCCA
GACGGCTCTGAGGTGGCGGGCGCCGCCCCCGCCCCCGGCCCGCCGCGCCAGCTGGTGGAG
GAGCTGCAGAGCCGCTACCGGCAGATGGAGGAACGCATCACCTGCCCCATCTGCATCGAC
AGCCACATCCGCCTCGTGTTCCAGTGCGGCCACGGCGCATGCGCCCCCTGCGGCTCCGCG
CTCAGCGCCTGCCCCATCTGCCGCCAGCCCATCCGCGACCGCATCCAGATCTTCGTG
Protein sequence predicted based on nucleotide sequence
MGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAAPPNMDPDPQAGVQVGMRVV RGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLL YDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETA HSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGR SVASVTWADGTTNVYRVGYKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQP FQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWT FHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNL RVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRL VVEVALGNAARALGLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDD EGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCD VNLPDAHSDTPLHSAISAGTGAGGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAV RKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAV QQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQ ASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRER QAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEEC ARRMKKCIRCQVVVSKKLRPDGSEVAGAAPAPGPPRQLVEELQSRYRQMEERITCPICID SHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
