Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
"insert_ID 28998,DNASU_Clone_ID HsCD00042305"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00824693
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2634nts
Open reading frame: 1 to 2634
ATGTTGACAAGACTGCAAGTTCTTACCTTAGCTTTGTTTTCAAAGGGATTTTTACTCTCT
TTAGGGGACCATAACTTTCTAAGGAGAGAGATTAAAATAGAAGGTGACCTTGTTTTAGGG
GGCCTGTTTCCTATTAACGAAAAAGGCACTGGAACTGAAGAATGTGGGCGAATCAATGAA
GACCGAGGGATTCAACGCCTGGAAGCCATGTTGTTTGCTATTGATGAAATCAACAAAGAT
GATTACTTGCTACCAGGAGTGAAGTTGGGTGTTCACATTTTGGATACATGTTCAAGGGAT
ACCTATGCATTGGAGCAATCACTGGAGTTTGTCAGGGCATCTTTGACAAAAGTGGATGAA
GCTGAGTATATGTGTCCTGATGGATCCTATGCCATTCAAGAAAACATCCCACTTCTCATT
GCAGGGGTCATTGGTGGCTCTTATAGCAGTGTTTCCATACAGGTGGCAAACCTGCTGCGG
CTCTTCCAGATCCCTCAGATCAGCTACGCATCCACCAGCGCCAAACTCAGTGATAAGTCG
CGCTATGATTACTTTGCCAGGACCGTGCCCCCCGACTTCTACCAGGCCAAAGCCATGGCT
GAGATCTTGCGCTTCTTCAACTGGACCTACGTGTCCACAGTAGCCTCCGAGGGTGATTAC
GGGGAGACAGGGATCGAGGCCTTCGAGCAGGAAGCCCGCCTGCGCAACATCTGCATCGCT
ACGGCGGAGAAGGTGGGCCGCTCCAACATCCGCAAGTCCTACGACAGCGTGATCCGAGAA
CTGTTGCAGAAGCCCAACGCGCGCGTCGTGGTCCTCTTCATGCGCAGCGACGACTCGCGG
GAGCTCATTGCAGCCGCCAGCCGCGCCAATGCCTCCTTCACCTGGGTGGCCAGCGACGGC
TGGGGCGCGCAGGAGAGCATCATCAAGGGCAGCGAGCATGTGGCCTACGGCGCCATCACC
CTGGAGCTGGCCTCCCAGCCTGTCCGCCAGTTCGACCGCTACTTCCAGAGCCTCAACCCC
TACAACAACCACCGCAACCCCTGGTTCCGGGACTTCTGGGAGCAAAAGTTTCAGTGCAGC
CTCCAGAACAAACGCAACCACAGGCGCGTCTGCGACAAGCACCTGGCCATCGACAGCAGC
AACTACGAGCAAGAGTCCAAGATCATGTTTGTGGTGAACGCGGTGTATGCCATGGCCCAC
GCTTTGCACAAAATGCAGCGCACCCTCTGTCCCAACACTACCAAGCTTTGTGATGCTATG
AAGATCCTGGATGGGAAGAAGTTGTACAAGGATTACTTGCTGAAAATCAACTTCACGGCT
CCATTCAACACAAATAAAGATGCAGATAGCATAGTCAAGTTTGACACTTTTGGAGATGGA
ATGGGGCGATACAACGTGTTCAATTTCCAAAATGTAGGTGGAAAGTATTCCTACTTGAAA
GTTGGTCACTGGGCAGAAACCTTATCGCTAGATGTCAACTCTATCCACTGGTCCCGGAAC
TCAGTCCCCACTTCCCAGTGCAGCGACGCCTGTGCCCCCAATGAAATGAAGAATATGCAA
CCAGGGGATGTCTGCTGCTGGATTTGCATCCCCTGTGAACCCTACGAATACCTGGCTGAT
GAGTTTACCTGTATGGATTGTGGGTCTGGACAGTGGCCCACTGCAGACCTAACTGGATGC
TATGACCTTCCTGAGGACTACATCAGGTGGGAAGACGCCTGGGCCATTGGCCCAGTCACC
ATTGCCTGTCTGGGTTTTATGTGTACATGCATGGTTGTAACTGTTTTTATCAAGCACAAC
AACACACCCTTGGTCAAAGCATCGGGCCGAGAACTCTGCTACATCTTATTGTTTGGGGTT
GGCCTGTCATACTGCATGACATTCTTCTTCATTGCCAAGCCATCACCAGTCATCTGTGCA
TTGCGCCGACTCGGGCTGGGGAGTTCCTTCGCTATCTGTTACTCAGCCCTGCTGACCAAG
ACAAACTGCATTGCCCGCATCTTCGATGGGGTCAAGAATGGCGCTCAGAGGCCAAAATTC
ATCAGCCCCAGTTCTCAGGTTTTCATCTGCCTGGGTCTGATCCTGGTGCAAATTGTGATG
GTGTCTGTGTGGCTCATCCTGGAGGCCCCAGGCACCAGGAGGTATACCCTTGCAGAGAAG
CGGGAAACAGTCATCCTAAAATGCAATGTCAAAGATTCCAGCATGTTGATCTCTCTTACC
TACGATGTGATCCTGGTGATCTTATGCACTGTGTACGCCTTCAAAACGCGGAAGTGCCCA
GAAAATTTCAACGAAGCTAAGTTCATAGGTTTTACCATGTACACCACGTGCATCATCTGG
TTGGCCTTCCTCCCTATATTTTATGTGACATCAAGTGACTACAGAGTGCAGACGACAACC
ATGTGCATCTCTGTCAGCCTGAGTGGCTTTGTGGTCTTGGGCTGTTTGTTTGCACCCAAG
GTTCACATCATCCTGTTTCAACCCCAGAAGAATGTTGTCACACACAGACTGCACCTCAAC
AGGTTCAGTGTCAGTGGAACTGGGACCACATACTCTCAGTACTCTGCAAGCACGTATGTG
CCAACGGTGTGCAATGGGCGGGAAGTCCTCGACTCCACCACCTCATCTCTGTTG
Protein sequence predicted based on nucleotide sequence
MLTRLQVLTLALFSKGFLLSLGDHNFLRREIKIEGDLVLGGLFPINEKGTGTEECGRINE DRGIQRLEAMLFAIDEINKDDYLLPGVKLGVHILDTCSRDTYALEQSLEFVRASLTKVDE AEYMCPDGSYAIQENIPLLIAGVIGGSYSSVSIQVANLLRLFQIPQISYASTSAKLSDKS RYDYFARTVPPDFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFEQEARLRNICIA TAEKVGRSNIRKSYDSVIRELLQKPNARVVVLFMRSDDSRELIAAASRANASFTWVASDG WGAQESIIKGSEHVAYGAITLELASQPVRQFDRYFQSLNPYNNHRNPWFRDFWEQKFQCS LQNKRNHRRVCDKHLAIDSSNYEQESKIMFVVNAVYAMAHALHKMQRTLCPNTTKLCDAM KILDGKKLYKDYLLKINFTAPFNTNKDADSIVKFDTFGDGMGRYNVFNFQNVGGKYSYLK VGHWAETLSLDVNSIHWSRNSVPTSQCSDACAPNEMKNMQPGDVCCWICIPCEPYEYLAD EFTCMDCGSGQWPTADLTGCYDLPEDYIRWEDAWAIGPVTIACLGFMCTCMVVTVFIKHN NTPLVKASGRELCYILLFGVGLSYCMTFFFIAKPSPVICALRRLGLGSSFAICYSALLTK TNCIARIFDGVKNGAQRPKFISPSSQVFICLGLILVQIVMVSVWLILEAPGTRRYTLAEK RETVILKCNVKDSSMLISLTYDVILVILCTVYAFKTRKCPENFNEAKFIGFTMYTTCIIW LAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKNVVTHRLHLN RFSVSGTGTTYSQYSASTYVPTVCNGREVLDSTTSSLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
