Gene:
Gene Symbol:
NRK
Gene Name:
Nik related kinase
Original Clone ID:
FLH267149.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082525
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGGACCTGGGGGCTGGAGGGACAGGGAGGTCACGGATCTGGGCCACCTGCCGGAT
CCAACTGGAATATTCTCACTAGATAAAACCATTGGCCTTGGTACTTATGGCAGAATCTAT
TTGGGACTTCATGAGAAGACTGGTGCATTTACAGCTGTTAAAGTGATGAACGCTCGTAAG
ACCCCTTTACCTGAAATAGGAAGGCGAGTGAGAGTGAATAAATATCAAAAATCTGTTGGG
TGGAGATACAGTGATGAGGAAGAGGATCTCAGGACTGAACTCAACCTTCTGAGGAAGTAC
TCTTTCCACAAAAACATTGTGTCCTTCTATGGAGCATTTTTCAAGCTGAGTCCCCCTGGT
CAGCGGCACCAACTTTGGATGGTGATGGAGTTATGTGCAGCAGGTTCGGTCACTGATGTA
GTGAGAATGACCAGTAATCAGAGTTTAAAAGAAGGTTGGATTGCTTATATCTGCCGAGAA
ATCCTTCAGGGCTTAGCTCACCTTCACGCACACCGAGTAATTCACCGGGACATCAAAGGT
CAGAATGTGCTGCTGACTCATAATGCTGAAGTAAAACTGGTTGATTTTGGAGTGAGTGCC
CAGGTGAGCAGAACTAATGGAAGAAGGAATAGTTTCATTGGGACACCATACTGGATGGCA
CCTGAGGTGATTGACTGTGATGAGGACCCAAGACGCTCCTATGATTACAGAAGTGATGTG
TGGTCTGTGGGAATTACTGCCATTGAAATGGCTGAAGGAGCCCCTCCTCTGTGTAACCTT
CAACCCTTGGAAGCTCTCTTCGTTATTTTGCGGGAATCTGCTCCCACAGTCAAATCCAGC
GGATGGTCCCGTAAGTTCCACAATTTCATGGAAAAGTGTACGATAAAAAATTTCCTGTTT
CGTCCTACTTCTGCAAACATGCTTCAACACCCATTTGTTCGGGATATAAAAAATGAACGA
CATGTTGTTGAGTCATTAACAAGGCATCTTACTGGAATCATTAAAAAAAGACAGAAAAAA
GGAATACCTTTGATCTTTGAAAGAGAAGAAGCTATTAAGGAACAGTACACCGTGAGAAGA
TTCAGAGGACCCTCTTGCACTCACGAGCTTCTGAGATTGCCAACCAGCAGCAGATGCAGA
CCACTTAGAGTCCTGCATGGGGAACCCTCTCAGCCAAGGTGGCTACCTGATCGAGAAGAG
CCACAGGTCCAGGCACTTCAGCAGCTACAGGGAGCAGCCAGGGTATTCATGCCACTGCAG
GCTCTGGACAGTGCACCTAAGCCTCTAAAGGGGCAGGCTCAGGCACCTCAACGACTACAA
GGGGCAGCTCGGGTGTTCATGCCACTACAGGCTCAGGTGAAGGCTAAGGCCTCTAAACCT
CTACAAATGCAGATTAAGGCACCTCCACGACTACGGAGGGCAGCCAGGGTGCTCATGCCA
CTACAGGCACAGGTTAGGGCACCTAGGCTTCTGCAGGTACAGTCCCAGGTATCCAAAAAG
CAGCAGGCCCAGACCCAGACATCAGAACCACAAGATTTGGACCAGGTACCAGAGGAATTT
CAGAGTCAAGATCAGGTACCCGAACAACAAAGGCAGGGCCAGGCCCCTGAACAACAGCAG
AGGCACAACCAGGTGCCTGAACAAGAGCTGGAGCAGAACCAGGCACCTGAACAGCCAGAG
GTACAGGAACAGGCTGCCGAGCCTGCACAGGCAGGGACTGAGGCAGAGGAACCTGAGTCA
TTACGAGTAAATGCCCAGGTATTTCTGCCCCTGCTATCACAAGATCACCATGTGCTGTTG
CCACTACATTTGGATACTCAGGTGCTCATTCCAGTAGAGGGGCAAACTGAAGGATCACCT
CAGGCACAGGCTTGGACACTAGAGCCCCCACAGGCAATTGGCTCAGTTCAAGCACTGATA
GAGGGACTATCAAGAGACTTGCTTCGGGCGCCAAACTCAAATAACTCAAAGCCACTTGGT
CCGTTGCAAACCCTGATGGAAAATCTGTCATCAAATAGGTTTTACTCACAACCAGAACAG
GCACGGGAGAAAAAATCAAAAGTTTCTACTCTGAGGCAAGCACTGGCAAAAAGACTATCA
CCAAAGAGGTTCGGGGCAAAGTCATCATGGAGACCTGAAAAGCTTGAACTCTCGGATTTA
GAAGCCCGCAGGCAAAGGCGCCAACGCAGATGGGAAGATATCTTTAATCAGCATGAGGAA
GAATTGAGACAAGTTGATAAAGACAAAGAAGATGAATCATCAGACAATGATGAAGTATTT
CATTCGATTCAGGCTGAAGTCCAGATAGAGCCATTGAAGCCATACATTTCAAATCCTAAA
AAAATTGAGGTTCAAGAGAGATCTCCTTCTGTGCCTAACAACCAGGATCATGCACATCAT
GTCAAGTTCTCTTCAAGCGTTCCTCAGCGGTCTCTTTTGGAACAAGCTCAGAAGCCCATT
GACATCAGACAAAGGAGTTCGCAAAATCGTCAAAATTGGCTGGCAGCATCAGAATCTTCT
TCTGAGGAAGAAAGTCCTGTGACTGGAAGGAGGTCTCAGTCATCACCACCTTATTCTACT
ATTGATCAGAAGTTGCTGGTTGACATCCATGTTCCAGATGGATTTAAAGTAGGAAAAATA
TCACCCCCTGTATACTTGACAAACGAATGGGTAGGCTATAATGCACTCTCTGAAATCTTC
CGGAATGATTGGTTAACTCCGGCACCTGTCATTCAGCCACCTGAAGAGGATGGTGATTAT
GTTGAACTCTATGATGCCAGTGCTGATACTGATGGTGATGATGATGATGAGTCTAATGAT
ACTTTTGAAGATACCTATGATCATGCCAATGGCAATGATGACTTGGATAACCAGGTTGAT
CAGGCTAATGATGTTTGTAAAGACCATGATGATGACAACAATAAGTTTGTTGATGATGTA
AATAATAATTATTATGAGGCGCCTAGTTGTCCAAGGGCAAGCTATGGCAGAGATGGAAGC
TGCAAGCAAGATGGTTATGATGGAAGTCGTGGAAAAGAGGAAGCCTACAGAGGCTATGGA
AGCCATACAGCCAATAGAAGCCATGGAGGAAGTGCAGCCAGTGAGGACAATGCAGCCATT
GGAGATCAGGAAGAACATGCAGCCAATATAGGCAGTGAAAGAAGAGGCAGTGAGGGTGAT
GGAGGTAAGGGAGTCGTTCGAACCAGTGAAGAGAGTGGAGCCCTTGGACTCAATGGAGAA
GAAAATTGCTCAGAGACAGATGGTCCAGGATTGAAGAGACCTGCGTCTCAGGACTTTGAA
TATCTACAGGAGGAGCCAGGTGGTGGAAATGAGGCCTCAAATGCCATTGACTCAGGTGCT
GCACCGTCAGCACCTGATCATGAGAGTGACAATAAGGACATATCAGAATCACCAACACAA
TCAGATTTTTCTGCCAATCACTCATCTCCTTCCAAAGGTTCTGGGATGTCTGCTGATGCT
AACTTTGCCAGTGCCATCTTATACGCTGGATTCGTAGAAGTACCTGAGGAATCACCTAAG
CAACCCTCTGAAGTCAATGTTAACCCACTCTATGTCTCTCCTGCATGTAAAAAACCACTA
ATCCACATGTATGAAAAGGAGTTCACTTCTGAGATCTGCTGCGGTTCTTTGTGGGGAGTC
AATTTGCTGTTGGGAACCCGATCTAATCTATATCTGATGGACAGAAGTGGAAAGGCTGAC
ATTACTAAACTTATAAGGCGAAGACCATTCCGCCAGATTCAAGTCTTAGAGCCACTCAAT
TTGCTGATTACCATCTCAGGTCATAAGAACAGACTTCGGGTGTATCATCTGACCTGGTTG
AGGAACAAGATTTTGAATAATGATCCAGAAAGTAAAAGAAGGCAAGAAGAAATGCTGAAG
ACAGAGGAAGCCTGCAAAGCTATTGATAAGTTAACAGGCTGTGAACACTTCAGTGTCCTC
CAACATGAAGAAACAACATATATTGCAATTGCTTTGAAATCATCAATTCACCTTTATGCA
TGGGCACCAAAGTCCTTTGATGAAAGCACTGCTATTAAAGTATGCATTGATCAATCAGCA
GACTCTGAAGGAGACTACATGTCCTATCAAGCCTATATACGAATACTGGCAAAAATACAG
GCAGCTGATCCAGTGAACCGGTTTAAGAGACCAGATGAGCTCCTTCATTTGCTGAAGCTC
AAGGTATTTCCAACACTTGATCATAAGCCAGTGACAGTTGACCTGGCTATTGGTTCTGAA
AAAAGACTAAAGATTTTCTTCAGCTCAGCAGATGGATATCACCTCATCGATGCAGAATCT
GAGGTTATGTCTGATGTGACCCTGCCAAAGAATCCCCTGGAAATCATTATACCACAGAAT
ATCATCATTTTACCTGATTGCTTGGGAATTGGCATGATGCTCACCTTCAATGCTGAAGCC
CTCTCTGTGGAAGCAAATGAACAACTCTTCAAGAAGATCCTTGAAATGTGGAAAGACATA
CCATCTTCTATAGCTTTTGAATGTACACAGCGAACCACAGGATGGGGCCAAAAGGCCATT
GAAGTGCGCTCTTTGCAATCCAGGGTTCTGGAAAGTGAGCTGAAGCGCAGGTCAATTAAG
AAGCTGAGATTCCTGTGCACCCGGGGTGACAAGCTGTTCTTTACCTCTACCCTGCGCAAT
CACCACAGCCGGGTTTACTTCATGACACTTGGAAAACTTGAAGAGCTCCAAAGCAATTAT
GATGTC
Protein sequence predicted based on nucleotide sequence
MAGPGGWRDREVTDLGHLPDPTGIFSLDKTIGLGTYGRIYLGLHEKTGAFTAVKVMNARK TPLPEIGRRVRVNKYQKSVGWRYSDEEEDLRTELNLLRKYSFHKNIVSFYGAFFKLSPPG QRHQLWMVMELCAAGSVTDVVRMTSNQSLKEGWIAYICREILQGLAHLHAHRVIHRDIKG QNVLLTHNAEVKLVDFGVSAQVSRTNGRRNSFIGTPYWMAPEVIDCDEDPRRSYDYRSDV WSVGITAIEMAEGAPPLCNLQPLEALFVILRESAPTVKSSGWSRKFHNFMEKCTIKNFLF RPTSANMLQHPFVRDIKNERHVVESLTRHLTGIIKKRQKKGIPLIFEREEAIKEQYTVRR FRGPSCTHELLRLPTSSRCRPLRVLHGEPSQPRWLPDREEPQVQALQQLQGAARVFMPLQ ALDSAPKPLKGQAQAPQRLQGAARVFMPLQAQVKAKASKPLQMQIKAPPRLRRAARVLMP LQAQVRAPRLLQVQSQVSKKQQAQTQTSEPQDLDQVPEEFQSQDQVPEQQRQGQAPEQQQ RHNQVPEQELEQNQAPEQPEVQEQAAEPAQAGTEAEEPESLRVNAQVFLPLLSQDHHVLL PLHLDTQVLIPVEGQTEGSPQAQAWTLEPPQAIGSVQALIEGLSRDLLRAPNSNNSKPLG PLQTLMENLSSNRFYSQPEQAREKKSKVSTLRQALAKRLSPKRFGAKSSWRPEKLELSDL EARRQRRQRRWEDIFNQHEEELRQVDKDKEDESSDNDEVFHSIQAEVQIEPLKPYISNPK KIEVQERSPSVPNNQDHAHHVKFSSSVPQRSLLEQAQKPIDIRQRSSQNRQNWLAASESS SEEESPVTGRRSQSSPPYSTIDQKLLVDIHVPDGFKVGKISPPVYLTNEWVGYNALSEIF RNDWLTPAPVIQPPEEDGDYVELYDASADTDGDDDDESNDTFEDTYDHANGNDDLDNQVD QANDVCKDHDDDNNKFVDDVNNNYYEAPSCPRASYGRDGSCKQDGYDGSRGKEEAYRGYG SHTANRSHGGSAASEDNAAIGDQEEHAANIGSERRGSEGDGGKGVVRTSEESGALGLNGE ENCSETDGPGLKRPASQDFEYLQEEPGGGNEASNAIDSGAAPSAPDHESDNKDISESPTQ SDFSANHSSPSKGSGMSADANFASAILYAGFVEVPEESPKQPSEVNVNPLYVSPACKKPL IHMYEKEFTSEICCGSLWGVNLLLGTRSNLYLMDRSGKADITKLIRRRPFRQIQVLEPLN LLITISGHKNRLRVYHLTWLRNKILNNDPESKRRQEEMLKTEEACKAIDKLTGCEHFSVL QHEETTYIAIALKSSIHLYAWAPKSFDESTAIKVCIDQSADSEGDYMSYQAYIRILAKIQ AADPVNRFKRPDELLHLLKLKVFPTLDHKPVTVDLAIGSEKRLKIFFSSADGYHLIDAES EVMSDVTLPKNPLEIIIPQNIIILPDCLGIGMMLTFNAEALSVEANEQLFKKILEMWKDI PSSIAFECTQRTTGWGQKAIEVRSLQSRVLESELKRRSIKKLRFLCTRGDKLFFTSTLRN HHSRVYFMTLGKLEELQSNYDV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
306
End on reference sequence
5054
Mutations:
None
Discrepancy:
a1564g,S522G; t2300c,V767A;
t2770c,Y924H; a3394g,K
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
