DNASU Plasmid | ASB4 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ASB4

Gene Name: ankyrin repeat and SOCS box containing 4

Original Clone ID:

FLH267379.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00082602

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1278nts

Open reading frame: 1 to 1278

Reference Sequence Alignment

ATGGACGGCACCACTGCCCCTGTCACTAAATCTGGAGCTGCCAAGTTAGTTAAGAGAAAT
TTCCTTGAGGCGCTAAAGTCCAATGACTTCGGAAAATTGAAGGCTATTTTGATCCAAAGG
CAAATAGATGTGGACACTGTTTTTGAAGTCGAAGATGAGAATATGGTTTTGGCATCTTAT
AAACAAGGTTACTGGTTGCCTAGCTATAAATTGAAGTCTTCCTGGGCCACAGGCCTCCAT
CTCTCTGTCTTGTTTGGCCATGTGGAATGTCTTCTGGTGCTACTGGACCACAATGCTACA
ATCAACTGTAGACCCAATGGGAAAACCCCTCTTCACGTGGCTTGTGAAATGGCCAATGTG
GATTGTGTTAAGATCCTCTGTGATCGTGGGGCAAAGCTCAATTGCTACTCCTTAAGTGGA
CACACAGCTTTGCACTTTTGTACAACTCCAAGTTCCATTCTCTGTGCCAAGCAATTGGTT
TGGAGAGGGGCGAATGTGAACATGAAGACCAACAACCAAGATGAGGAGACGCCCTTGCAC
ACGGCTGCCCACTTCGGCCTTTCGGAGCTGGTGGCCTTCTACGTGGAACACGGGGCCATA
GTGGACAGCGTGAATGCCCACATGGAGACCCCCCTGGCCATCGCCGCCTACTGGGCCCTC
CGCTTTAAGGAGCAGGAGTACAGCACGGAGCACCACCTGGTCTGCCGCATGCTGCTTGAC
TACAAAGCCGAAGTCAATGCCCGAGATGACGACTTTAAATCTCCCCTCCACAAGGCAGCC
TGGAACTGTGACCACGTGCTCATGCACATGATGCTGGAAGCTGGCGCCGAAGCCAATCTC
ATGGATATCAACGGCTGTGCTGCCATCCAGTACGTGCTGAAGGTCACCTCCGTGCGCCCT
GCTGCCCAGCCTGAGATCTGCTACCAGCTCCTGTTGAACCATGGGGCTGCCCGAATATAC
CCTCCACAGTTCCATAAGGTGATACAGGCCTGCCATTCTTGTCCTAAAGCAATTGAAGTT
GTAGTCAATGCCTATGAACACATCAGATGGAACACAAAGTGGAGAAGAGCTATCCCCGAT
GATGACTTGGAGAAATACTGGGATTTTTACCACTCTCTCTTTACTGTGTGCTGTAACTCT
CCAAGGACTCTCATGCACTTATCGAGATGTGCCATTAGAAGAACATTACACAACAGATGC
CATAGAGCAATTCCTTTGCTTTCCCTCCCATTGTCATTGAAAAAGTACTTGCTTTTAGAG
CCAGAGGGAATTATTTAT

Protein sequence predicted based on nucleotide sequence

MDGTTAPVTKSGAAKLVKRNFLEALKSNDFGKLKAILIQRQIDVDTVFEVEDENMVLASY
KQGYWLPSYKLKSSWATGLHLSVLFGHVECLLVLLDHNATINCRPNGKTPLHVACEMANV
DCVKILCDRGAKLNCYSLSGHTALHFCTTPSSILCAKQLVWRGANVNMKTNNQDEETPLH
TAAHFGLSELVAFYVEHGAIVDSVNAHMETPLAIAAYWALRFKEQEYSTEHHLVCRMLLD
YKAEVNARDDDFKSPLHKAAWNCDHVLMHMMLEAGAEANLMDINGCAAIQYVLKVTSVRP
AAQPEICYQLLLNHGAARIYPPQFHKVIQACHSCPKAIEVVVNAYEHIRWNTKWRRAIPD
DDLEKYWDFYHSLFTVCCNSPRTLMHLSRCAIRRTLHNRCHRAIPLLSLPLSLKKYLLLE
PEGIIY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1315

Start on reference sequence 35

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

ASB4

Symbol Nomenclature :

ASB4

SYNONYM :

ASB-4

Designation :

ankyrin repeat and SOCS box protein 4|ankyrin repeat and SOCS box-containing 4

Full Nomenclature :

ankyrin repeat and SOCS box containing 4

GENEID :

51666

GI :

109939881

GenBank Accession :

BC111594

HGNC :

16009

MIM :

605761

Vega :

OTTHUMG00000153960

Target GenBank:

BC111594

Reference Sequence Alignment

  

HsCD00082602      ATGGACGGCACCACTGCCCCTGTCACTAAATCTGGAGCTGCCAAGTTAGTTAAGAGAAAT
NM_016116.2       ATGGACGGCACCACTGCCCCTGTCACTAAATCTGGAGCTGCCAAGTTAGTTAAGAGAAAT
                  ************************************************************

HsCD00082602      TTCCTTGAGGCGCTAAAGTCCAATGACTTCGGAAAATTGAAGGCTATTTTGATCCAAAGG
NM_016116.2       TTCCTTGAGGCGCTAAAGTCCAATGACTTCGGAAAATTGAAGGCTATTTTGATCCAAAGG
                  ************************************************************

HsCD00082602      CAAATAGATGTGGACACTGTTTTTGAAGTCGAAGATGAGAATATGGTTTTGGCATCTTAT
NM_016116.2       CAAATAGATGTGGACACTGTTTTTGAAGTCGAAGATGAGAATATGGTTTTGGCATCTTAT
                  ************************************************************

HsCD00082602      AAACAAGGTTACTGGTTGCCTAGCTATAAATTGAAGTCTTCCTGGGCCACAGGCCTCCAT
NM_016116.2       AAACAAGGTTACTGGTTGCCTAGCTATAAATTGAAGTCTTCCTGGGCCACAGGCCTCCAT
                  ************************************************************

HsCD00082602      CTCTCTGTCTTGTTTGGCCATGTGGAATGTCTTCTGGTGCTACTGGACCACAATGCTACA
NM_016116.2       CTCTCTGTCTTGTTTGGCCATGTGGAATGTCTTCTGGTGCTACTGGACCACAATGCTACA
                  ************************************************************

HsCD00082602      ATCAACTGTAGACCCAATGGGAAAACCCCTCTTCACGTGGCTTGTGAAATGGCCAATGTG
NM_016116.2       ATCAACTGTAGACCCAATGGGAAAACCCCTCTTCACGTGGCTTGTGAAATGGCCAATGTG
                  ************************************************************

HsCD00082602      GATTGTGTTAAGATCCTCTGTGATCGTGGGGCAAAGCTCAATTGCTACTCCTTAAGTGGA
NM_016116.2       GATTGTGTTAAGATCCTCTGTGATCGTGGGGCAAAGCTCAATTGCTACTCCTTAAGTGGA
                  ************************************************************

HsCD00082602      CACACAGCTTTGCACTTTTGTACAACTCCAAGTTCCATTCTCTGTGCCAAGCAATTGGTT
NM_016116.2       CACACAGCTTTGCACTTTTGTACAACTCCAAGTTCCATTCTCTGTGCCAAGCAATTGGTT
                  ************************************************************

HsCD00082602      TGGAGAGGGGCGAATGTGAACATGAAGACCAACAACCAAGATGAGGAGACGCCCTTGCAC
NM_016116.2       TGGAGAGGGGCGAATGTGAACATGAAGACCAACAACCAAGATGAGGAGACGCCCTTGCAC
                  ************************************************************

HsCD00082602      ACGGCTGCCCACTTCGGCCTTTCGGAGCTGGTGGCCTTCTACGTGGAACACGGGGCCATA
NM_016116.2       ACGGCTGCCCACTTCGGCCTTTCGGAGCTGGTGGCCTTCTACGTGGAACACGGGGCCATA
                  ************************************************************

HsCD00082602      GTGGACAGCGTGAATGCCCACATGGAGACCCCCCTGGCCATCGCCGCCTACTGGGCCCTC
NM_016116.2       GTGGACAGCGTGAATGCCCACATGGAGACCCCCCTGGCCATCGCCGCCTACTGGGCCCTC
                  ************************************************************

HsCD00082602      CGCTTTAAGGAGCAGGAGTACAGCACGGAGCACCACCTGGTCTGCCGCATGCTGCTTGAC
NM_016116.2       CGCTTTAAGGAGCAGGAGTACAGCACGGAGCACCACCTGGTCTGCCGCATGCTGCTTGAC
                  ************************************************************

HsCD00082602      TACAAAGCCGAAGTCAATGCCCGAGATGACGACTTTAAATCTCCCCTCCACAAGGCAGCC
NM_016116.2       TACAAAGCCGAAGTCAATGCCCGAGATGACGACTTTAAATCTCCCCTCCACAAGGCAGCC
                  ************************************************************

HsCD00082602      TGGAACTGTGACCACGTGCTCATGCACATGATGCTGGAAGCTGGCGCCGAAGCCAATCTC
NM_016116.2       TGGAACTGTGACCACGTGCTCATGCACATGATGCTGGAAGCTGGCGCCGAAGCCAATCTC
                  ************************************************************

HsCD00082602      ATGGATATCAACGGCTGTGCTGCCATCCAGTACGTGCTGAAGGTCACCTCCGTGCGCCCT
NM_016116.2       ATGGATATCAACGGCTGTGCTGCCATCCAGTACGTGCTGAAGGTCACCTCCGTGCGCCCT
                  ************************************************************

HsCD00082602      GCTGCCCAGCCTGAGATCTGCTACCAGCTCCTGTTGAACCATGGGGCTGCCCGAATATAC
NM_016116.2       GCTGCCCAGCCTGAGATCTGCTACCAGCTCCTGTTGAACCATGGGGCTGCCCGAATATAC
                  ************************************************************

HsCD00082602      CCTCCACAGTTCCATAAGGTGATACAGGCCTGCCATTCTTGTCCTAAAGCAATTGAAGTT
NM_016116.2       CCTCCACAGTTCCATAAGGTGATACAGGCCTGCCATTCTTGTCCTAAAGCAATTGAAGTT
                  ************************************************************

HsCD00082602      GTAGTCAATGCCTATGAACACATCAGATGGAACACAAAGTGGAGAAGAGCTATCCCCGAT
NM_016116.2       GTAGTCAATGCCTATGAACACATCAGATGGAACACAAAGTGGAGAAGAGCTATCCCCGAT
                  ************************************************************

HsCD00082602      GATGACTTGGAGAAATACTGGGATTTTTACCACTCTCTCTTTACTGTGTGCTGTAACTCT
NM_016116.2       GATGACTTGGAGAAATACTGGGATTTTTACCACTCTCTCTTTACTGTGTGCTGTAACTCT
                  ************************************************************

HsCD00082602      CCAAGGACTCTCATGCACTTATCGAGATGTGCCATTAGAAGAACATTACACAACAGATGC
NM_016116.2       CCAAGGACTCTCATGCACTTATCGAGATGTGCCATTAGAAGAACATTACACAACAGATGC
                  ************************************************************

HsCD00082602      CATAGAGCAATTCCTTTGCTTTCCCTCCCATTGTCATTGAAAAAGTACTTGCTTTTAGAG
NM_016116.2       CATAGAGCAATTCCTTTGCTTTCCCTCCCATTGTCATTGAAAAAGTACTTGCTTTTAGAG
                  ************************************************************

HsCD00082602      CCAGAGGGAATTATTTATTAA
NM_016116.2       CCAGAGGGAATTATTTATTAA
                  *********************