Gene:
Gene Symbol:
GLTSCR1
Gene Name:
glioma tumor suppressor candidate region gene 1
Original Clone ID:
FLH269613.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082834
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGCTCAACACGCCCACCTCCCAGCTCCTGGCCAAGCAGGTGCCCGTCAGCGGCTAC
CTGGCCTCGGCGGCTGGCCCCTCGGAGCCCGTGACGCTGGCGTCGGCCGGTGTCTCGCCA
CAGGGGGCTGGCCTGGTCATCCAGAAGAACCTCTCGGCCGCTGTGGCCACCACGCTCAAT
GGGAACTCTGTGTTCGGAGGCGCGGGGGCCGCCTCGGCTCCCACCGGGACGCCCTCGGGA
CAGCCGCTGGCGGTGGCCCCAGGCCTCGGCTCGTCGCCACTGGTCCCGGCGCCCAACGTG
ATCCTGCATCGCACACCCACGCCCATCCAGCCCAAGCCCGCGGGGGTGCTGCCGCCCAAG
CTCTACCAGCTGACGCCCAAGCCGTTTGCGCCCGCGGGCGCCACGCTCACCATCCAGGGC
GAGCCGGGGGCGCTCCCGCAGCAGCCCAAGGCCCCGCAGAACCTGACGTTCATGGCGGCG
GGGAAGGCGGGCCAGAACGTGGTGCTGTCGGGCTTCCCCGCGCCTGCGCTGCAAGCGAAC
GTCTTCAAGCAGCCACCGGCCACCACCACCGGAGCGGCCCCGCCGCAGCCCCCCGGGGCC
CTGAGCAAACCCATGAGCGTCCACCTCCTGAACCAAGGCAGCAGCATCGTCATCCCCGCC
CAGCACATGCTGCCGGGCCAGAACCAGTTCCTACTGCCTGGCGCCCCGGCGGTCCAGCTC
CCGCAGCAGCTCTCAGCCCTGCCGGCCAACGTGGGCGGGCAGATCCTGGCGGCCGCTGCC
CCCCACACAGGTGGACAGCTCATCGCGAACCCCATCCTCACAAACCAGAACCTGGCGGGC
CCACTGAGCCTGGGCCCCGTGTTGGCCCCCCACTCCGGGGCCCACAGCGCGCACATCCTC
TCCGCCGCTCCCATCCAGGTGGGCCAGCCTGCGCTCTTCCAGATGCCCGTGTCGCTGGCG
GCGGGCAGCCTGCCCACGCAGAGCCAGCCAGCGCCCGCCGGGCCGGCCGCCACCACTGTC
CTCCAGGGGGTCACCCTGCCCCCCAGCGCCGTGGCCATGCTCAACACCCCCGACGGCCTG
GTGCAGCCGGCCACCCCTGCCGCTGCCACCGGGGAGGCCGCGCCTGTCCTCACGGTGCAG
CCTGCCCCCCAGGCGCCCCCCGCGGTCAGCACACCCCTGCCCCTGGGCCTCCAGCAGCCG
CAGGCGCAGCAGCCCCCGCAGGCCCCCACCCCACAGGCCGCCGCCCCGCCTCAGGCCACC
ACCCCCCAGCCCAGCCCTGGCCTGGCGTCTAGCCCGGAGAAGATCGTCCTGGGGCAGCCG
CCCTCTGCCACCCCCACGGCCATCCTCACTCAGGACTCCCTGCAGATGTTCCTGCCCCAG
GAGAGGAGCCAGCAGCCCCTCTCCGCAGAGGGCCCCCACCTCTCCGTGCCTGCCTCGGTC
ATAGTCAGCGCCCCGCCTCCCGCCCAAGACCCAGCCCCAGCCACCCCCGTCGCCAAAGGA
GCTGGCCTCGGCCCTCAGGCCCCCGACAGCCAGGCTTCCCCGGCTCCGGCCCCCCAGATC
CCGGCAGCGGCTCCGCTGAAGGGCCCAGGCCCCTCTTCGTCCCCGTCACTACCTCACCAG
GCCCCTCTGGGGGACAGCCCCCACCTGCCCTCCCCACACCCCACCCGGCCCCCTTCCCGC
CCACCCTCCCGGCCACAGAGTGTGTCCCGCCCTCCCTCAGAGCCACCCTTGCACCCTTGC
CCCCCACCCCAGGCCCCCCCAACTCTGCCTGGCATCTTTGTCATCCAAAACCAGCTAGGC
GTTCCCCCGCCTGCCAGCAACCCGGCCCCTACTGCCCCAGGCCCGCCGCAGCCGCCTCTC
CGCCCCCAGTCCCAGCCGCCTGAGGGACCGCTGCCCCCAGCCCCCCACCTCCCTCCATCC
TCCACCTCCTCTGCTGTGGCCTCCTCCTCTGAGACGTCCTCCAGGTTGCCAGCCCCTACG
CCATCCGACTTCCAGCTCCAGTTCCCACCCAGCCAGGGGCCCCACAAGTCCCCCACTCCC
CCTCCAACCCTCCACCTGGTCCCTGAGCCGGCAGCACCCCCCCCACCGCCTCCTCGGACC
TTCCAGATGGTGACCACCCCCTTCCCAGCGCTGCCCCAGCCGAAGGCTCTTCTCGAGAGA
TTTCACCAGGTGCCGTCCGGAATCATCCTCCAGAACAAGGCTGGGGGGGCCCCTGCCGCC
CCGCAGACCTCCACCAGCCTGGGGCCCCTCACCAGCCCCGCTGCGTCTGTGCTGGTCAGT
GGGCAGGCCCCATCTGGGACCCCCACTGCCCCCAGCCACGCCCCCGCCCCGGCACCCATG
GCCGCCACAGGCCTCCCTCCTCTGCTTCCAGCCGAGAACAAGGCTTTTGCCAGCAACCTC
CCGACCCTGAATGTGGCCAAGGCCGCTTCCTCCGGGCCAGGGAAGCCCTCCGGGCTGCAG
TATGAGAGCAAACTGAGTGGCCTGAAGAAGCCCCCCACGCTTCAGCCCAGCAAGGAAGCC
TGTTTCCTGGAGCATTTGCACAAACACCAGGGCTCCGTCCTGCACCCCGACTACAAGACG
GCCTTCCCCTCCTTTGAGGACGCCCTGCATCGCCTCCTGCCCTACCATGTCTACCAGGGC
GCCCTCCCCTCCCCCAGTGACTACCACAAAGTGGACGAGGAGTTTGAGACGGTCTCCACG
CAGCTGCTGAAACGCACCCAGGCCATGCTCAATAAATATCGGCTCCTGCTCCTGGAGGAG
TCCCGGAGGGTGAGCCCCTCAGCGGAGATGGTAATGATCGACCGAATGTTCATTCAGGAG
GAGAAGACCACCCTTGCCTTGGATAAACAGCTGGCCAAGGAGAAGCCGGACGAGTACGTG
TCTTCCTCCCGCTCGCTCGGCCTCCCCATCGCAGCCTCTTCCGAGGGTCATCGGCTTCCC
GGCCACGGCCCCCTGTCGTCTTCAGCTCCCGGGGCCTCCACCCAGCCCCCTCCACACCTG
CCCACCAAGCTTGTGATCCGGCACGGCGGGGCAGGCGGCTCCCCTTCGGTCACCTGGGCC
CGGGCGTCCTCCTCCCTGTCCTCCTCTTCCTCCTCCTCCTCTGCCGCCTCCTCCTTGGAC
GCCGACGAGGACGGCCCCATGCCCTCCCGCAACCGCCCGCCCATCAAGACCTACGAGGCC
CGGAGCCGCATCGGGCTCAAGCTCAAGATCAAGCAGGAAGCCGGGCTCAGCAAGGTCGTG
CACAACACGGCCCTGGACCCCGTGCACCAGCCCCCGCCACCCCCCGCTACCCTCAAGGTG
GCCGAGCCCCCGCCACGGCCGCCACCACCACCGCCGCCCACGGGCCAGATGAACGGCACG
GTGGACCACCCGCCGCCTGCCGCCCCCGAGCGCAAGCCCCTGGGCACCGCCCCGCACTGC
CCGCGCCTGCCACTGCGCAAGACCTACCGCGAGAACGTGGGGGGCCCTGGCGCGCCGGAG
GGGACGCCCGCAGGCAGGGCACGGGGAGGCAGCCCGGCGCCGCTGCCCGCCAAAGTGGAC
GAGGCCACCAGCGGGCTCATCCGCGAGCTGGCGGCCGTGGAGGACGAGCTGTACCAGCGT
ATGCTGAAGGGCCCCCCGCCAGAGCCCGCAGCCAGCGCCGCCCAAGGCACCGGGGACCCC
GACTGGGAGGCGCCCGGGCTGCCCCCTGCCAAGCGGCGCAAGTCCGAGTCGCCCGACGTG
GACCAGGCCAGCTTCTCCAGCGACAGCCCGCAGGATGACACGCTCACCGAGCACCTGCAG
AGCGCCATCGACAGCATCCTGAACCTGCAGCAGGCCCCCGGCCGGACGCCCGCGCCCTCG
TACCCCCACGCTGCCTCGGCCGGCACCCCCGCATCCCCGCCGCCCCTGCACAGGCCCGAG
GCCTACCCACCCTCCAGTCACAACGGTGGCCTCGGCGCCAGGACGTTGACCAGA
Protein sequence predicted based on nucleotide sequence
MALNTPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLSAAVATTLN GNSVFGGAGAASAPTGTPSGQPLAVAPGLGSSPLVPAPNVILHRTPTPIQPKPAGVLPPK LYQLTPKPFAPAGATLTIQGEPGALPQQPKAPQNLTFMAAGKAGQNVVLSGFPAPALQAN VFKQPPATTTGAAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGAPAVQL PQQLSALPANVGGQILAAAAPHTGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAHIL SAAPIQVGQPALFQMPVSLAAGSLPTQSQPAPAGPAATTVLQGVTLPPSAVAMLNTPDGL VQPATPAAATGEAAPVLTVQPAPQAPPAVSTPLPLGLQQPQAQQPPQAPTPQAAAPPQAT TPQPSPGLASSPEKIVLGQPPSATPTAILTQDSLQMFLPQERSQQPLSAEGPHLSVPASV IVSAPPPAQDPAPATPVAKGAGLGPQAPDSQASPAPAPQIPAAAPLKGPGPSSSPSLPHQ APLGDSPHLPSPHPTRPPSRPPSRPQSVSRPPSEPPLHPCPPPQAPPTLPGIFVIQNQLG VPPPASNPAPTAPGPPQPPLRPQSQPPEGPLPPAPHLPPSSTSSAVASSSETSSRLPAPT PSDFQLQFPPSQGPHKSPTPPPTLHLVPEPAAPPPPPPRTFQMVTTPFPALPQPKALLER FHQVPSGIILQNKAGGAPAAPQTSTSLGPLTSPAASVLVSGQAPSGTPTAPSHAPAPAPM AATGLPPLLPAENKAFASNLPTLNVAKAASSGPGKPSGLQYESKLSGLKKPPTLQPSKEA CFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPSDYHKVDEEFETVST QLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYV SSSRSLGLPIAASSEGHRLPGHGPLSSSAPGASTQPPPHLPTKLVIRHGGAGGSPSVTWA RASSSLSSSSSSSSAASSLDADEDGPMPSRNRPPIKTYEARSRIGLKLKIKQEAGLSKVV HNTALDPVHQPPPPPATLKVAEPPPRPPPPPPPTGQMNGTVDHPPPAAPERKPLGTAPHC PRLPLRKTYRENVGGPGAPEGTPAGRARGGSPAPLPAKVDEATSGLIRELAAVEDELYQR MLKGPPPEPAASAAQGTGDPDWEAPGLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQ SAIDSILNLQQAPGRTPAPSYPHAASAGTPASPPPLHRPEAYPPSSHNGGLGARTLTR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4892
Start on reference sequence
936
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
