ZNF135 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00829266
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2046nts
Open reading frame: 1 to 2046

ATGGAGTTAGGCTCGCGCCGGCGCAGTGTCGGCTGCCGGTGCCGCGGCCTTTGTCTCGCA
GTCAGGAGGGAGCAAGTGACGTTTGAGGACGTGGTAGTGGGCTTCAGCCAGGAGGAGTGG
GGGCAGCTGAAGCCTGCCCAGAGGACCCTGTACCGTGATGTAATGCTGGACACCTTCAGG
CTTCTGGTCTCTGTGGGACATTGGTTACCGAAGCCGAATGTCATCTCCCTGCTGGAGCAA
GAGGCAGAGCTGTGGGCGGTGGAGTCTAGACTTCCCCAAGGCGTGTACCCAGAGATTAAG
GGACATTTCCAGTTTTTGCTTCTTTCAGACTTGGAAACTAGACCCAAAGTCAAACTGTCA
GTTCTAAAGCAAGGCATCTCTGAAGAAATATCCAACAGTGTCATCTTGGTAGAAAGATTC
CTGTGGGATGGTCTGTGGTACTGCAGGGGTGAGGACACTGAGGGCCACTGGGAATGGAGT
TGTGAGAGTCTAGAGAGCCTGGCAGTGCCGGTGGCCTTCACGCCTGTGAAGACGCCTGTT
CTGGAGCAGTGGCAGAGGAATGGGTTTGGGGAAAACATAAGTCTGAACCCTGATCTCCCA
CATCAACCAATGACTCCTGAAAGACAAAGCCCCCACACATGGGGAACACGTGGAAAAAGG
GAGAAGCCAGACCTAAATGTTTTACAGAAAACCTGTGTAAAAGAGAAACCCTACAAATGT
CAGGAATGCGGAAAGGCCTTTAGTCACAGCTCAGCACTTATCGAACACCACCGGACGCAC
ACAGGAGAGAGACCTTACGAATGTCACGAATGCTTAAAAGGCTTCCGGAACAGCTCGGCA
CTTACCAAACACCAGAGAATCCATACTGGGGAGAAACCCTATAAATGCACTCAGTGTGGG
AGGACCTTCAACCAAATTGCCCCACTGATCCAGCACCAGAGAACTCACACAGGTGAGAAG
CCCTATGAATGCAGCGAATGTGGGAAATCCTTCAGTTTTAGGTCCTCCTTCAGCCAGCAC
GAGCGAACTCACACAGGCGAGAAGCCCTACGAGTGCAGTGAGTGTGGGAAAGCCTTCCGG
CAAAGCATCCACCTCACCCAGCATCTGCGAATCCACACTGGGGAGAAACCCTATCAGTGT
GGTGAGTGTGGCAAGGCCTTCAGCCACAGCTCATCCTTGACCAAACACCAGCGAATCCAC
ACAGGGGAGAAGCCCTACGAGTGCCATGAGTGTGGAAAAGCCTTCACCCAGATCACACCA
CTGATTCAGCACCAGAGGACCCACACAGGAGAAAAGCCCTATGAGTGTGGTGAGTGTGGG
AAAGCCTTCAGTCAGAGCACACTCCTGACCGAGCATCGGAGGATTCACACAGGAGAGAAG
CCCTATGGATGCAACGAGTGTGGGAAAACCTTCAGCCACAGCTCCTCACTCAGCCAGCAT
GAGCGGACACACACAGGAGAGAAGCCCTATGAGTGCAGTCAGTGTGGGAAGGCCTTCCGG
CAGAGCACACACCTCACCCAACACCAGCGAATCCACACAGGGGAGAAGCCCTATGAATGC
AATGACTGCGGCAAGGCATTCAGTCACAGCTCGTCCCTCACCAAACATCAGCGAATCCAC
ACTGGGGAGAAGCCCTACGAATGCAACCAGTGTGGCAGAGCCTTCAGCCAGCTTGCTCCC
CTCATTCAGCATCAGAGGATCCACACAGGAGAGAAACCCTATGAATGTAACCAGTGTGGC
AGAGCCTTCAGCCAGAGCTCCCTTCTCATCGAACACCAGAGGATTCACACCAAGGAAAAG
CCGTATGGGTGCAATGAGTGTGGGAAATCCTTCAGCCACAGCTCCTCGCTCAGCCAGCAC
GAAAGGACGCACACTGGGGAAAAGCCCTATGAGTGTCACGATTGCGGAAAGTCCTTTAGG
CAGAGCACCCACCTCACTCAGCACCGGAGGATCCACACAGGAGAGAAGCCATATGCATGC
AGGGACTGTGGAAAGGCCTTTACCCACAGCTCCTCCCTTACCAAGCACCAGAGAACTCAC
ACTGGATTG

Protein sequence predicted based on nucleotide sequence

MELGSRRRSVGCRCRGLCLAVRREQVTFEDVVVGFSQEEWGQLKPAQRTLYRDVMLDTFR
LLVSVGHWLPKPNVISLLEQEAELWAVESRLPQGVYPEIKGHFQFLLLSDLETRPKVKLS
VLKQGISEEISNSVILVERFLWDGLWYCRGEDTEGHWEWSCESLESLAVPVAFTPVKTPV
LEQWQRNGFGENISLNPDLPHQPMTPERQSPHTWGTRGKREKPDLNVLQKTCVKEKPYKC
QECGKAFSHSSALIEHHRTHTGERPYECHECLKGFRNSSALTKHQRIHTGEKPYKCTQCG
RTFNQIAPLIQHQRTHTGEKPYECSECGKSFSFRSSFSQHERTHTGEKPYECSECGKAFR
QSIHLTQHLRIHTGEKPYQCGECGKAFSHSSSLTKHQRIHTGEKPYECHECGKAFTQITP
LIQHQRTHTGEKPYECGECGKAFSQSTLLTEHRRIHTGEKPYGCNECGKTFSHSSSLSQH
ERTHTGEKPYECSQCGKAFRQSTHLTQHQRIHTGEKPYECNDCGKAFSHSSSLTKHQRIH
TGEKPYECNQCGRAFSQLAPLIQHQRIHTGEKPYECNQCGRAFSQSSLLIEHQRIHTKEK
PYGCNECGKSFSHSSSLSQHERTHTGEKPYECHDCGKSFRQSTHLTQHRRIHTGEKPYAC
RDCGKAFTHSSSLTKHQRTHTG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable