Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00829444
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4569nts
Open reading frame: 1 to 4569
ATGGCGGCCGTAGGGTCCGGAGGCTATGCGCGGAACGATGCAGGGGAGAAGCTGCCCTCT
GTCATGGCGGGAGTTCCGGCGCGGAGGGGCCAGTCCTCCCCGCCCCCCGCCCCACCAATC
TGCCTACGGCGGCGGACGCGACTCTCGACGGCCTCCGAGGAGACGGTGCAGAACCGGGTG
TCACTCGAGAAGGTGCTTGGCATCACAGCCCAGAACAGCAGTGGCCTAACCTGTGACCCC
GGCACAGGCCATGTGGCCTACCTGGCAGGCTGTGTGGTGGTGATTTTGGACCCCAAGGAG
AACAAGCAGCAGCACATCTTTAACACCGCCAGGAAGTCTCTCAGTGCTCTGGCCTTCTCC
CCTGATGGGAAGTACATAGTGACAGGGGAGAATGGGCATAGGCCTGCTGTGCGCATCTGG
GATGTGGAGGAGAAGAATCAGGTGGCGGAGATGCTAGGCCACAAGTATGGTGTGGCGTGT
GTGGCCTTCTCACCCAATATGAAGCACATCGTGTCCATGGGCTACCAACATGACATGGTG
CTCAACGTCTGGGACTGGAAGAAAGACATCGTAGTGGCCTCCAACAAGGTATCTTGTAGA
GTCATTGCCCTCTCCTTCTCAGAGGACAGCAGCTATTTTGTCACTGTTGGGAACCGCCAT
GTGAGGTTCTGGTTCTTGGAAGTCTCCACTGAGACAAAGGTGACGAGCACAGTGCCCCTT
GTAGGGCGCTCGGGCATCCTGGGCGAGCTGCACAACAACATCTTCTGTGGTGTGGCCTGC
GGTCGGGGCCGGATGGCGGGCAGTACCTTCTGTGTGTCCTACTCGGGCCTCCTCTGCCAG
TTCAATGAGAAGAGGGTGCTGGAGAAGTGGATCAACCTGAAGGTCTCCCTGTCTTCCTGC
CTCTGTGTCAGCCAGGAGCTCATCTTCTGTGGCTGCACAGATGGGATAGTCCGCATCTTC
CAGGCCCATAGCCTGCACTACCTCGCCAACCTGCCCAAGCCACACTACCTTGGGGTAGAC
GTGGCACAGGGCCTGGAGCCCAGCTTCCTCTTCCACAGGAAGGCGGAAGCAGTCTACCCA
GATACAGTGGCACTGACCTTCGACCCCATCCACCAGTGGCTGTCCTGCGTGTATAAGGAC
CACAGCATCTACATCTGGGATGTCAAGGACATCAACAGAGTGGGCAAGGTGTGGTCAGAG
CTCTTCCACAGCTCCTACGTTTGGAACGTGGAGGTGTATCCTGAGTTTGAAGACCAGAGA
GCTTGTTTGCCATCAGGATCCTTTCTGACTTGTTCTTCAGACAACACCATTCGCTTCTGG
AACTTGGACAGCAGCCCTGATTCTCACTGGCAGAAAAACATCTTCAGCAATACCCTGCTG
AAGGTCGTGTACGTGGAGAATGACATCCAGCACCTGCAGGACATGTCACACTTCCCAGAC
CGGGGGAGCGAGAATGGGACACCCATGGACGTGAAAGCCGGGGTGCGGGTCATGCAGGTC
AGTCCTGACGGCCAGCATTTGGCTTCAGGCGACCGAAGTGGAAATCTGAGGATCCACGAG
CTGCACTTCATGGACGAGCTGGTCAAGGTGGAGGCCCATGATGCTGAGGTGCTGTGCCTG
GAGTACTCCAAGCCAGAGACGGGGCTGACCTTGCTGGCCTCAGCCAGTCGGGACCGGCTG
ATCCATGTGCTGAACGTGGAGAAGAACTACAACCTGGAGCAGACGCTGGATGACCACTCC
TCCTCCATCACCGCCATCAAGTTCGCTGGCAACAGAGACATCCAGATGATCAGCTGTGGG
GCTGACAAGAGCATCTACTTTCGCAGTGCCCAGCAGGGTTCGGATGGACTACACTTTGTC
CGTACCCACCACGTAGCAGAGAAAACCACCTTGTATGACATGGACATTGACATCACCCAG
AAGTACGTGGCCGTGGCCTGCCAGGACCGCAATGTGAGAGTCTACAACACTGTGAACGGG
AAGCAGAAGAAGTGCTACAAGGGCTCCCAGGGTGACGAAGGGTCCTTGCTGAAGGTCCAT
GTGGACCCCTCAGGCACCTTCCTGGCCACCAGCTGCTCTGACAAAAGCATCTCAGTGATT
GACTTTTACTCGGGCGAGTGCATTGCCAAGATGTTTGGCCATTCAGAAATTATTACCAGC
ATGAAGTTCACCTATGACTGTCATCACTTGATCACAGTATCTGGAGACAGCTGCGTGTTC
ATCTGGCACCTGGGCCCGGAGATCACCAACTGCATGAAGCAGCACTTGCTGGAGATTGAC
CACCGGCAGCAGCAGCAGCACACAAATGACAAGAAGCGGAGTGGCCACCCCAGGCAGGAT
ACGTATGTGTCCACACCTAGTGAGATTCACTCCCTGAGCCCTGGAGAGCAAACAGAGGAT
GATCTGGAGGAAGAGTGTGAGCCAGAAGAGATGCTGAAGACACCATCCAAAGATAGCTTG
GATCCAGATCCTCGTTGCCTGCTAACCAACGGCAAGCTGCCACTGTGGGCAAAGCGGCTG
CTAGGGGACGATGATGTGGCAGATGGCTTGGCCTTCCACGCCAAGCGCAGCTACCAGCCC
CACGGCCGCTGGGCAGAGCGGGCCGGCCAAGAGCCCCTCAAGACCATCCTGGATGCCCAG
GACCTGGATTGCTACTTTACCCCCATGAAGCCCGAGAGTCTGGAGAACTCCATTCTGGAT
TCACTGGAGCCACAGAGCCTGGCCAGCCTGCTGAGTGAGTCAGAGAGTCCCCAGGAAGCT
GGCCGCGGGCACCCCTCCTTCCTGCCCCAGCAGAAGGAATCATCTGAGGCCAGTGAGCTC
ATCCTCTACTCTCTGGAGGCAGAAGTGACAGTCACAGGGACAGACAGCCAGTATTGCAGG
AAGGAGGTGGAGGCCGGGCCTGGAGACCAGCAGGGCGACTCCTACCTCAGGGTGTCCTCC
GACAGCCCAAAGGACCAGAGCCCGCCTGAGGACTCGGGGGAGTCAGAGGCCGACCTGGAG
TGCAGCTTCGCAGCCATCCACTCCCCAGCTCCGCCTCCTGACCCTGCCCCTCGGTTTGCC
ACGTCGCTGCCCCATTTCCCAGGATGCGCAGGTCCCACAGAAGATGAGCTGTCCCTGCCC
GAGGGACCCAGCGTCCCCAGCAGCTCCCTACCCCAGACTCCGGAGCAGGAGAAGTTCCTC
CGCCACCACTTTGAGACACTGACTGAGTCCCCCTGCAGAGAGCTCTTCCCCGCAGCTCTG
GGAGACGTGGAGGCCTCTGAAGCTGAAGACCACTTCTTCAACCCACGCCTGAGTATCTCC
ACGCAGTTCCTCTCAAGCCTCCAGAAGGCATCCAGGTTCACCCATACCTTCCCTCCCCGG
GCAACCCAGTGCCTTGTGAAGTCTCCAGAGGTCAAGCTCATGGACCGAGGCGGAAGCCAG
CCCAGAGCAGGTACTGGCTACGCCTCCCCAGACAGGACCCACGTCCTCGCTGCAGGGAAG
GCTGAAGAGACCCTGGAGGCCTGGCGCCCACCACCTCCCTGCCTTACGAGCCTGGCGTCC
TGTGTCCCTGCTTCCTCCGTGCTGCCCACAGACAGGAATCTCCCAACGCCCACATCTGCA
CCCACCCCAGGCCTGGCTCAGGGTGTCCATGCCCCCTCCACCTGTTCCTACATGGAGGCC
ACTGCCAGCTCCCGTGCCAGGATATCACGCAGCATCTCCCTCGGTGACAGTGAGGGCCCT
ATCGTGGCCACACTGGCCCAGCCCCTCCGTAGGCCATCGTCCGTTGGGGAGCTGGCCTCC
TTGGGCCAGGAGCTTCAGGCCATCACCACCGCGACAACACCCAGTTTGGACAGTGAGGGC
CAAGAGCCTGCCCTGCGTTCCTGGGGCAACCACGAGGCCCGGGCCAACCTGAGACTGACC
CTGTCAAGTGCCTGTGATGGGCTCCTGCAGCCCCCCGTGGATACCCAGCCTGGCGTCACC
GTCCCTGCAGTGAGCTTCCCAGCCCCTAGCCCTGTGGAAGAGAGCGCCCTGAGGCTCCAC
GGCTCTGCCTTTCGCCCAAGTCTCCCAGCTCCTGAGTCCCCTGGCCTTCCTGCCCACCCC
AGTAACCCCCAGCTTCCAGAGGCCCGGCCTGGCATCCCTGGCGGCACTGCCTCCCTCCTG
GAGCCCACCTCCGGTGCACTTGGTCTGTTACAGGGCAGCCCTGCCCGCTGGAGTGAGCCC
TGGGTGCCGGTTGAAGCCCTGCCCCCATCTCCCCTTGAGCTGAGCAGGGTGGGGAACATC
TTGCACAGGCTGCAGACCACCTTCCAAGAAGCCCTCGACCTTTACCGTGTGTTGGTCTCC
AGTGGCCAGGTGGACACCGGGCAGCAGCAGGCACGGACTGAGCTGGTCTCCACCTTCCTG
TGGATCCACAGCCAGCTGGAGGCTGAATGCCTGGTGGGGACTAGTGTGGCCCCAGCCCAG
GCTCTGCCCAGCCCAGGACCCCCGTCCCCACCGACGCTGTACCCCCTGGCCAGCCCAGAC
CTGCAGGCCCTGCTGGAACACTACTCGGAGCTGCTGGTGCAGGCCGTGCGGAGGAAGGCA
CGGGGGCACTTG
Protein sequence predicted based on nucleotide sequence
MAAVGSGGYARNDAGEKLPSVMAGVPARRGQSSPPPAPPICLRRRTRLSTASEETVQNRV SLEKVLGITAQNSSGLTCDPGTGHVAYLAGCVVVILDPKENKQQHIFNTARKSLSALAFS PDGKYIVTGENGHRPAVRIWDVEEKNQVAEMLGHKYGVACVAFSPNMKHIVSMGYQHDMV LNVWDWKKDIVVASNKVSCRVIALSFSEDSSYFVTVGNRHVRFWFLEVSTETKVTSTVPL VGRSGILGELHNNIFCGVACGRGRMAGSTFCVSYSGLLCQFNEKRVLEKWINLKVSLSSC LCVSQELIFCGCTDGIVRIFQAHSLHYLANLPKPHYLGVDVAQGLEPSFLFHRKAEAVYP DTVALTFDPIHQWLSCVYKDHSIYIWDVKDINRVGKVWSELFHSSYVWNVEVYPEFEDQR ACLPSGSFLTCSSDNTIRFWNLDSSPDSHWQKNIFSNTLLKVVYVENDIQHLQDMSHFPD RGSENGTPMDVKAGVRVMQVSPDGQHLASGDRSGNLRIHELHFMDELVKVEAHDAEVLCL EYSKPETGLTLLASASRDRLIHVLNVEKNYNLEQTLDDHSSSITAIKFAGNRDIQMISCG ADKSIYFRSAQQGSDGLHFVRTHHVAEKTTLYDMDIDITQKYVAVACQDRNVRVYNTVNG KQKKCYKGSQGDEGSLLKVHVDPSGTFLATSCSDKSISVIDFYSGECIAKMFGHSEIITS MKFTYDCHHLITVSGDSCVFIWHLGPEITNCMKQHLLEIDHRQQQQHTNDKKRSGHPRQD TYVSTPSEIHSLSPGEQTEDDLEEECEPEEMLKTPSKDSLDPDPRCLLTNGKLPLWAKRL LGDDDVADGLAFHAKRSYQPHGRWAERAGQEPLKTILDAQDLDCYFTPMKPESLENSILD SLEPQSLASLLSESESPQEAGRGHPSFLPQQKESSEASELILYSLEAEVTVTGTDSQYCR KEVEAGPGDQQGDSYLRVSSDSPKDQSPPEDSGESEADLECSFAAIHSPAPPPDPAPRFA TSLPHFPGCAGPTEDELSLPEGPSVPSSSLPQTPEQEKFLRHHFETLTESPCRELFPAAL GDVEASEAEDHFFNPRLSISTQFLSSLQKASRFTHTFPPRATQCLVKSPEVKLMDRGGSQ PRAGTGYASPDRTHVLAAGKAEETLEAWRPPPPCLTSLASCVPASSVLPTDRNLPTPTSA PTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELAS LGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLQPPVDTQPGVT VPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGGTASLL EPTSGALGLLQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVS SGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPD LQALLEHYSELLVQAVRRKARGH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
