Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00829501
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4497nts
Open reading frame: 1 to 4497
ATGATGATGGCAAGAAAGCAAGATGTCCGAATTCCCACCTACAACATCAGTGTGGTGGGA
TTATCTGGGACCGAGAAGGAAAAGGGCCAGTGTGGGATTGGAAAGTCTTGTTTGTGCAAC
CGCTTCGTGCGCCCGAGTGCTGACGAGTTTCACTTGGACCATACCTCCGTCCTCAGCACC
AGTGACTTTGGAGGGCGAGTGGTCAATAATGACCACTTTCTCTACTGGGGAGAAGTTAGC
CGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTGTGGAGCAGACTGAATTTATT
GATGATCAGACTTTTCAACCTCATCGAAGCACGGCCCTGCAGCCCTATATCAAGAGAGCT
GCTGCGACCAAGCTTGCATCAGCTGAAAAACTCATGTACTTTTGCACTGACCAGCTGGGG
CTGGAGCAGGACTTTGAGCAGAAACAAATGCCAGACGGAAAGCTGCTGGTTGATGGTTTT
CTTCTTGGTATTGATGTTAGCAGGGGCATGAATAGGAACTTTGATGACCAGCTCAAGTTT
GTCTCCAATCTCTACAATCAGCTTGCAAAAACAAAAAAGCCCATAGTGGTGGTCCTGACT
AAGTGTGACGAAGGTGTTGAGCGGTACATTAGAGATGCACATACTTTTGCCTTAAGCAAA
AAGAACCTCCAGGTTGTGGAGACCTCAGCGAGATCCAATGTAAACGTGGACTTGGCTTTC
AGCACCTTAGTGCAACTCATTGATAAAAGTCGGGGAAAGACAAAAATCATTCCTTATTTT
GAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAAGACAAGTATGAGTGGCTG
GTGAGTCGCATTGTGAAAAACCACAATGAGAACTGGCTGAGTGTCAGCCGAAAGATGCAG
GCCTCTCCAGAATACCAGGACTATGTCTACCTGGAAGGGACTCAGAAAGCCAAGAAGCTG
TTTCTACAGCACATCCACCGCCTCAAGCATGAGCATATCGAGCGTAGGAGAAAGCTGTAC
CTGGCAGCCCTGCCATTAGCTTTTGAAGCTCTTATACCTAATCTAGATGAAATAGACCAC
CTAAGCTGCATAAAAGCCAAAAAGCTCTTAGAAACCAAGCCAGAATTCTTGAAGTGGTTT
GTTGTGCTTGAAGAGACCCCATGGGATGCCACCAGTCACATTGACAACATGGAAAACGAA
CGGATTCCCTTTGATTTAATGGATACCGTCCCTGCAGAGCAGCTATACGAGGCCCACTTA
GAGAAGCTGAGGAACGAAAGGAAAAGAGTTGAGATGCGAAGGGCGTTTAAAGAAAACCTG
GAGACTTCTCCTTTCATAACTCCCGGAAAGCCTTGGGAAGAGGCCCGTAGTTTTATTATG
AATGAGGATTTCTACCAGTGGCTGGAGGAATCTGTATACATGGATATTTATGGCAAACAC
CAAAAGCAAATTATAGATAAAGCAAAGGAAGAATTTCAGGAGTTGCTTTTGGAATATTCA
GAATTGTTTTATGAACTGGAGCTGGATGCTAAGCCCAGCAAGGAGAAGATGGGTGTTATT
CAGGATGTTCTGGGAGAGGAACAGCGATTTAAAGCATTACAAAAGCTCCAAGCAGAGCGT
GATGCCCTTATTCTGAAACACATTCATTTTGTGTACCACCCAACAAAGGAGACATGCCCC
AGCTGCCCAGCTTGTGTGGACGCTAAGATTGAGCACTTGATTAGTTCTCGGTTTATCCGG
CCGTCTGACCGGAATCAGAAAAATTCACTCTCTGACCCTAACATTGATAGAATCAACTTG
GTTATATTGGGCAAAGACGGCCTTGCCCGAGAGTTGGCCAATGAGATTCGAGCTCTTTGT
ACAAATGATGACAAGTATGTGATAGATGGTAAAATGTATGAGCTTTCCCTGAGGCCAATA
GAGGGGAATGTCAGGCTTCCTGTGAACTCTTTCCAGACGCCAACATTTCAGCCCCACGGC
TGTCTCTGCCTTTACAATTCAAAGGAATCGCTATCCTATGTAGTGGAAAGTATAGAGAAG
AGTAGAGAGTCCACGCTGGGCCGGCGGGATAATCATTTAGTCCATCTCCCCCTTACATTA
ATTTTGGTTAACAAGAGAGGAGACACCAGTGGAGAGACTCTGCATAGCTTAATACAGCAA
GGTCAACAAATTGCTAGCAAACTTCAGTGTGTCTTTCTCGACCCTGCTTCTGCTGGCATT
GGTTACGGACGCAACATTAATGAAAAGCAAATCAGTCAAGTTTTGAAGGGACTCCTGGAC
TCTAAGCGTAACTTAAACCTGGTCAGTTCTACTGCTAGCATCAAAGATTTGGCTGATGTT
GATCTGCGAATTGTTATGTGTCTGATGTGTGGAGATCCTTTTAGTGCAGATGACATACTT
TTTCCTGTCCTTCAGTCCCAAACCTGTAAATCTTCCCATTGTGGAAGCAACAACTCTGTT
TTACTTGAACTACCAATCGGACTGCACAAGAAGCGGATTGAACTGTCTGTTCTTTCATAC
CATTCCTCCTTTAGCATCAGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCA
GCCAAACGTAAGGCCTCTTTGGCTATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATT
ATCCCTATTCAGCTTGTAGCACTCACTGATGGCGCTGTAGATGTCCTGGACAATGACTTA
AGTAGGGAACAGCTAACTGAGGGGGAGGAGATTGCTCAAGAAATTGACGGAAGGTTCACA
AGCATCCCCTGTAGCCAACCCCAGCATAAACTTGAGATCTTTCACCCATTTTTTAAAGAT
GTGGTGGAAAAAAAGAACATAATCGAGGCTACTCATATGTACGATAATGCTGCCGAGGCC
TGTAGCACCACCGAAGAGGTGTTTAACTCCCCCCGGGCAGGATCACCGCTCTGCAACTCA
AACCTGCAGGATTCAGAAGAAGATATCGAGCCATCTTACAGCCTGTTTCGAGAAGACACA
TCACTGCCTTCTCTGTCCAAAGACCATTCTAAGCTCTCTATGGAACTGGAGGGAAATGAT
GGGCTGTCTTTCATTATGAGCAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCGCCA
GTCAAACCAAAGCCTCCTGTCCATTTTGAAATTACAAAGGGGGATCTATCTTATTTAGAC
CAAGGCCATAGGGATGGACAGAGGAAGTCTGTGTCTTCTAGCCCCTGGCTGCCTCAGGAT
GGGTTTGATCCTTCTGACTATGCTGAACCCATGGATGCTGTGGTGAAGCCAAGGAATGAA
GAAGAAAACATATACTCCGTGCCCCATGACAGCACCCAAGGCAAAATCATCACCATTCGG
AATATCAACAAAGCCCAGTCCAACGGCAGCGGGAATGGTTCTGACAGTGAAATGGACACC
AGCTCTCTAGAGCGAGGGCGCAAGGTTTCCATCGTGAGCAAGCCAGTGCTGTACAGGACG
AGATGCACCCGGCTGGGGCGGTTTGCTAGTTACCGGACCAGCTTCAGCGTGGGGAGTGAT
GATGAGCTGGGGCCCATCCGGAAGAAAGAGGAGGATCAGGCATCCCAGGGTTATAAAGGG
GACAATGCTGTCATTCCATACGAAACAGACGAAGACCCGCGGAGGAGGAATATTCTTCGC
AGCCTAAGGAGGAACACTAAGAAACCAAAGCCCAAACCCCGGCCATCCATCACAAAGGCA
ACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACTGTCGTGACTCCAGAGAAGCCG
ATCCCCATTTTTATTGAAAGATGTATTGAGTACATTGAAGCCACAGGACTGAGCACGGAA
GGCATCTACCGGGTCAGCGGGAACAAGTCTGAGATGGAGAGTCTGCAGAGACAGTTTGAT
CAAGACCACAACCTGGACCTGGCAGAGAAAGACTTTACGGTGAATACCGTGGCTGGTGCC
ATGAAGAGCTTTTTCTCAGAACTGCCTGACCCCCTGGTCCCGTATAACATGCAGATCGAC
TTGGTGGAAGCACACAAAATCAACGACCGGGAGCAGAAGTTGCATGCCCTTAAGGAGGTA
TTAAAGAAATTTCCAAAGGAAAACCACGAAGTCTTCAAGTATGTCATCTCTCACCTAAAC
AAGGTCAGCCACAACAACAAGGTGAATCTCATGACCAGCGAGAACCTCTCCATCTGCTTC
TGGCCCACCTTGATGAGACCTGATTTCAGCACTATGGACGCCCTCACAGCCACGCGCACC
TACCAGACAATCATTGAACTCTTTATCCAGCAGTGCCCCTTCTTCTTCTACAATCGGCCC
ATCACCGAGCCCCCCGGCGCCAGGCCCAGCTCCCCCTCTGCCGTGGCTTCCACCGTCCCC
TTCCTCACTTCCACGCCTGTCACAAGTCAGCCGTCGCCCCCACAGTCGCCTCCACCCACC
CCCCAGTCCCCAATGCAGCCACTGCTTCCCTCCCAGCTTCAAGCCGAACACACGCTGTTG
Protein sequence predicted based on nucleotide sequence
MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLST SDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRA AATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKF VSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAF STLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQ ASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDH LSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHL EKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKH QKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAER DALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINL VILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHG CLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQ GQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADV DLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSY HSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDL SREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEA CSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGND GLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQD GFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDT SSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKG DNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKP IPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGA MKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLN KVSHNNKVNLMTSENLSICFWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFFYNRP ITEPPGARPSSPSAVASTVPFLTSTPVTSQPSPPQSPPPTPQSPMQPLLPSQLQAEHTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
