Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00829505
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5805nts
Open reading frame: 1 to 5805
ATGCAGTTTGTATCCTGGGCCACACTGCTAACGCTCCTGGTGCGGGACCTGGCCGAGATG
GGGAGCCCAGACGCCGCGGCGGCCGTGCGCAAGGACAGGCTGCACCCGAGGCAAGTGAAA
TTATTAGAGACCCTGAGCGAATACGAAATCGTGTCTCCCATCCGAGTGAACGCTCTCGGA
GAACCCTTTCCCACGAACGTCCACTTCAAAAGAACGCGACGGAGCATTAACTCTGCCACT
GACCCCTGGCCTGCCTTCGCCTCCTCCTCTTCCTCCTCTACCTCCTCCCAGGCGCATTAC
CGCCTCTCTGCCTTCGGCCAGCAGTTTCTATTTAATCTCACCGCCAATGCCGGATTTATC
GCTCCACTGTTCACTGTCACCCTCCTCGGGACGCCCGGGGTGAATCAGACCAAGTTTTAT
TCCGAAGAGGAAGCGGAACTCAAGCACTGTTTCTACAAAGGCTATGTCAATACCAACTCC
GAGCACACGGCCGTCATCAGCCTCTGCTCAGGAATGCTGGGCACATTCCGGTCTCATGAT
GGGGATTATTTTATTGAACCACTACAGTCTATGGATGAACAAGAAGATGAAGAGGAACAA
AACAAACCCCACATCATTTATAGGCGCAGCGCCCCCCAGAGAGAGCCCTCAACAGGAAGG
CATGCATGTGACACCTCAGAACACAAAAATAGGCACAGTAAAGACAAGAAGAAAACCAGA
GCAAGAAAATGGGGAGAAAGGATTAACCTGGCTGGTGACGTAGCAGCATTAAACAGCGGC
TTAGCAACAGAGGCATTTTCTGCTTATGGTAATAAGACGGACAACACAAGAGAAAAGAGG
ACCCACAGAAGGACAAAACGTTTTTTATCCTATCCACGGTTTGTAGAAGTCTTGGTGGTG
GCAGACAACAGAATGGTTTCATACCATGGAGAAAACCTTCAACACTATATTTTAACTTTA
ATGTCAATTGTAGCCTCTATCTATAAAGACCCAAGTATTGGAAATTTAATTAATATTGTT
ATTGTGAACTTAATTGTGATTCATAATGAACAGGATGGGCCTTCCATATCTTTTAATGCT
CAGACAACATTAAAAAACTTTTGCCAGTGGCAGCATTCGAAGAACAGTCCAGGTGGAATC
CATCATGATACTGCTGTTCTCTTAACAAGACAGGATATCTGCAGAGCTCACGACAAATGT
GATACCTTAGGCCTGGCTGAACTGGGAACCATTTGTGATCCCTATAGAAGCTGTTCTATT
AGTGAAGATAGTGGATTGAGTACAGCTTTTACGATCGCCCATGAGCTGGGCCATGTGTTT
AACATGCCTCATGATGACAACAACAAATGTAAAGAAGAAGGAGTTAAGAGTCCCCAGCAT
GTCATGGCTCCAACACTGAACTTCTACACCAACCCCTGGATGTGGTCAAAGTGTAGTCGA
AAATATATCACTGAGTTTTTAGACACTGGTTATGGCGAGTGTTTGCTTAACGAACCTGAA
TCCAGACCCTACCCTTTGCCTGTCCAACTGCCAGGCATCCTTTACAACGTGAATAAACAA
TGTGAATTGATTTTTGGACCAGGTTCTCAGGTGTGCCCATATATGATGCAGTGCAGACGG
CTCTGGTGCAATAACGTCAATGGAGTACACAAAGGCTGCCGGACTCAGCACACACCCTGG
GCCGATGGGACGGAGTGCGAGCCTGGAAAGCACTGCAAGTATGGATTTTGTGTTCCCAAA
GAAATGGATGTCCCCGTGACAGATGGATCCTGGGGAAGTTGGAGTCCCTTTGGAACCTGC
TCCAGAACATGTGGAGGGGGCATCAAAACAGCCATTCGAGAGTGCAACAGACCAGAACCA
AAAAATGGTGGAAAATACTGTGTAGGACGTAGAATGAAATTTAAGTCCTGCAACACGGAG
CCATGTCTCAAGCAGAAGCGAGACTTCCGAGATGAACAGTGTGCTCACTTTGACGGGAAG
CATTTTAACATCAACGGTCTGCTTCCCAATGTGCGCTGGGTCCCTAAATACAGTGGAATT
CTGATGAAGGACCGGTGCAAGTTGTTCTGCAGAGTGGCAGGGAACACAGCCTACTATCAG
CTTCGAGACAGAGTGATAGATGGAACTCCTTGTGGCCAGGACACAAATGATATCTGTGTC
CAGGGCCTTTGCCGGCAAGCTGGATGCGATCATGTTTTAAACTCAAAAGCCCGGAGAGAT
AAATGTGGGGTTTGTGGTGGCGATAATTCTTCATGCAAAACAGTGGCAGGAACATTTAAT
ACAGTACATTATGGTTACAATACTGTGGTCCGAATTCCAGCTGGTGCTACCAATATTGAT
GTGCGGCAGCACAGTTTCTCAGGGGAAACAGACGATGACAACTACTTAGCTTTATCAAGC
AGTAAAGGTGAATTCTTGCTAAATGGAAACTTTGTTGTCACAATGGCCAAAAGGGAAATT
CGCATTGGGAATGCTGTGGTAGAGTACAGTGGGTCCGAGACTGCCGTAGAAAGAATTAAC
TCAACAGATCGCATTGAGCAAGAACTTTTGCTTCAGGTTTTGTCGGTGGGAAAGTTGTAC
AACCCCGATGTACGCTATTCTTTCAATATTCCAATTGAAGATAAACCTCAGCAGTTTTAC
TGGAACAGTCATGGGCCATGGCAAGCATGCAGTAAACCCTGCCAAGGGGAACGGAAACGA
AAACTTGTTTGCACCAGGGAATCTGATCAGCTTACTGTTTCTGATCAAAGATGCGATCGG
CTGCCCCAGCCTGGACACATTACTGAACCCTGTGGTACAGACTGTGACCTGAGGTGGCAT
GTTGCCAGCAGGAGTGAATGTAGTGCCCAGTGTGGCTTGGGTTACCGCACATTGGACATC
TACTGTGCCAAATATAGCAGGCTGGATGGGAAGACTGAGAAGGTTGATGATGGTTTTTGC
AGCAGCCATCCCAAACCAAGCAACCGTGAAAAATGCTCAGGGGAATGTAACACGGGTGGC
TGGCGCTATTCTGCCTGGACTGAATGTTCAAAAAGCTGTGACGGTGGGACCCAGAGGAGA
AGGGCTATTTGTGTCAATACCCGAAATGATGTACTGGATGACAGCAAATGCACACATCAA
GAGAAAGTTACCATTCAGAGGTGCAGTGAGTTCCCTTGTCCACAGTGGAAATCTGGAGAC
TGGTCAGAGTGCTTGGTCACCTGTGGAAAAGGGCATAAGCACCGCCAGGTCTGGTGTCAG
TTTGGTGAAGATCGATTAAATGATAGAATGTGTGACCCTGAGACCAAGCCAACATCTATG
CAGACTTGTCAGCAGCCGGAATGTGCATCCTGGCAGGCGGGTCCCTGGGGACAGTGCAGT
GTCACTTGTGGACAGGGATACCAGCTAAGAGCAGTGAAATGCATCATTGGGACTTATATG
TCAGTGGTAGATGACAATGACTGTAATGCAGCAACTAGACCAACTGATACCCAGGACTGT
GAATTACCATCATGTCATCCTCCCCCAGCTGCCCCGGAAACGAGGAGAAGCACATACAGT
GCACCAAGAACCCAGTGGCGATTTGGGTCTTGGACCCCATGCTCAGCCACTTGTGGGAAA
GGTACCCGGATGAGATACGTCAGCTGCCGAGATGAGAATGGCTCTGTGGCTGACGAGAGT
GCCTGTGCTACCCTGCCTAGACCAGTGGCAAAGGAAGAATGTTCTGTGACACCCTGTGGG
CAATGGAAGGCCTTGGACTGGAGCTCTTGCTCTGTGACCTGTGGGCAAGGTAGGGCAACC
CGGCAAGTGATGTGTGTCAACTACAGTGACCACGTGATCGATCGGAGTGAGTGTGACCAG
GATTATATCCCAGAAACTGACCAGGACTGTTCCATGTCACCATGCCCTCAAAGGACCCCA
GACAGTGGCTTAGCTCAGCACCCCTTCCAAAATGAGGACTATCGTCCCCGGAGCGCCAGC
CCCAGCCGCACCCATGTGCTCGGTGGAAACCAGTGGAGAACTGGCCCCTGGGGAGCATGT
TCCAGTACCTGTGCTGGCGGATCCCAGCGGCGTGTTGTTGTATGTCAGGATGAAAATGGA
TACACCGCAAACGACTGTGTGGAGAGAATAAAACCTGATGAGCAAAGAGCCTGTGAATCC
GGCCCTTGTCCTCAGTGGGCTTATGGCAACTGGGGAGAGTGCACTAAGCTGTGTGGTGGA
GGCATAAGAACAAGACTGGTGGTCTGTCAGCGGTCCAACGGTGAACGGTTTCCAGATTTG
AGCTGTGAAATTCTTGATAAACCTCCCGATCGTGAGCAGTGTAACACACATGCTTGTCCA
CACGACGCTGCATGGAGTACTGGCCCTTGGAGCTCGTGTTCTGTCTCTTGTGGTCGAGGG
CATAAACAACGAAATGTTTACTGCATGGCAAAAGATGGAAGCCATTTAGAAAGTGATTAC
TGTAAGCACCTGGCTAAGCCACATGGGCACAGAAAGTGCCGAGGAGGAAGATGCCCCAAA
TGGAAAGCTGGCGCTTGGAGTCAGTGCTCTGTGTCCTGTGGCCGAGGCGTACAGCAGAGG
CATGTGGGCTGTCAGATCGGAACACACAAAATAGCCAGAGAGACCGAGTGCAACCCATAC
ACCAGACCGGAGTCGGAACGCGACTGCCAAGGCCCACGGTGTCCCCTCTACACTTGGAGG
GCAGAGGAATGGCAAGAATGCACCAAGACCTGCGGCGAAGGCTCCAGGTACCGCAAGGTG
GTGTGTGTGGATGACAACAAAAACGAGGTGCATGGGGCACGCTGTGACGTGAGCAAGCGG
CCGGTGGACCGTGAAAGCTGTAGTTTGCAACCCTGCGAGTATGTCTGGATCACAGGAGAA
TGGTCAGAGTGCTCAGTGACCTGTGGAAAAGGCTACAAACAAAGGCTTGTCTCGTGCAGC
GAGATTTACACCGGGAAGGAGAATTATGAATACAGCTACCAAACCACCATCAACTGCCCA
GGCACGCAGCCCCCCAGTGTTCACCCCTGTTACCTGAGGGACTGCCCTGTCTCGGCCACC
TGGAGAGTTGGCAACTGGGGGAGCTGCTCAGTGTCTTGTGGTGTTGGAGTGATGCAGAGA
TCTGTGCAATGTTTAACCAATGAGGACCAACCCAGCCACTTATGCCACACTGATCTGAAG
CCAGAAGAACGAAAAACCTGCCGTAATGTCTATAACTGTGAGTTACCCCAGAATTGCAAG
GAGGTAAAAAGACTTAAAGGTGCCAGTGAAGATGGTGAATATTTCCTGATGATTAGAGGA
AAGCTTCTGAAGATATTCTGTGCGGGGATGCACTCTGACCACCCCAAAGAGTACGTGACA
CTGGTGCATGGAGACTCTGAGAATTTCTCCGAGGTTTATGGGCACAGGTTACACAACCCA
ACAGAATGTCCCTATAACGGGAGCCGGCGCGATGACTGCCAATGTCGGAAGGATTACACG
GCCGCTGGGTTTTCCAGTTTTCAGAAAATCAGAATAGACCTGACCAGCATGCAGATAATC
ACCACTGACTTACAGTTTGCAAGGACAAGCGAAGGACATCCCGTCCCTTTTGCCACAGCC
GGGGATTGCTACAGCGCTGCCAAGTGCCCACAGGGTCGTTTTAGCATCAACCTTTATGGA
ACCGGCTTGTCTTTAACTGAATCTGCCAGATGGATATCACAAGGGAATTATGCTGTCTCT
GACATCAAGAAGTCGCCGGATGGTACCCGAGTCGTAGGGAAATGCGGTGGTTACTGTGGA
AAATGCACTCCATCCTCTGGTACTGGCCTGGAGGTGCGAGTTTTATTG
Protein sequence predicted based on nucleotide sequence
MQFVSWATLLTLLVRDLAEMGSPDAAAAVRKDRLHPRQVKLLETLSEYEIVSPIRVNALG EPFPTNVHFKRTRRSINSATDPWPAFASSSSSSTSSQAHYRLSAFGQQFLFNLTANAGFI APLFTVTLLGTPGVNQTKFYSEEEAELKHCFYKGYVNTNSEHTAVISLCSGMLGTFRSHD GDYFIEPLQSMDEQEDEEEQNKPHIIYRRSAPQREPSTGRHACDTSEHKNRHSKDKKKTR ARKWGERINLAGDVAALNSGLATEAFSAYGNKTDNTREKRTHRRTKRFLSYPRFVEVLVV ADNRMVSYHGENLQHYILTLMSIVASIYKDPSIGNLINIVIVNLIVIHNEQDGPSISFNA QTTLKNFCQWQHSKNSPGGIHHDTAVLLTRQDICRAHDKCDTLGLAELGTICDPYRSCSI SEDSGLSTAFTIAHELGHVFNMPHDDNNKCKEEGVKSPQHVMAPTLNFYTNPWMWSKCSR KYITEFLDTGYGECLLNEPESRPYPLPVQLPGILYNVNKQCELIFGPGSQVCPYMMQCRR LWCNNVNGVHKGCRTQHTPWADGTECEPGKHCKYGFCVPKEMDVPVTDGSWGSWSPFGTC SRTCGGGIKTAIRECNRPEPKNGGKYCVGRRMKFKSCNTEPCLKQKRDFRDEQCAHFDGK HFNINGLLPNVRWVPKYSGILMKDRCKLFCRVAGNTAYYQLRDRVIDGTPCGQDTNDICV QGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCKTVAGTFNTVHYGYNTVVRIPAGATNID VRQHSFSGETDDDNYLALSSSKGEFLLNGNFVVTMAKREIRIGNAVVEYSGSETAVERIN STDRIEQELLLQVLSVGKLYNPDVRYSFNIPIEDKPQQFYWNSHGPWQACSKPCQGERKR KLVCTRESDQLTVSDQRCDRLPQPGHITEPCGTDCDLRWHVASRSECSAQCGLGYRTLDI YCAKYSRLDGKTEKVDDGFCSSHPKPSNREKCSGECNTGGWRYSAWTECSKSCDGGTQRR RAICVNTRNDVLDDSKCTHQEKVTIQRCSEFPCPQWKSGDWSECLVTCGKGHKHRQVWCQ FGEDRLNDRMCDPETKPTSMQTCQQPECASWQAGPWGQCSVTCGQGYQLRAVKCIIGTYM SVVDDNDCNAATRPTDTQDCELPSCHPPPAAPETRRSTYSAPRTQWRFGSWTPCSATCGK GTRMRYVSCRDENGSVADESACATLPRPVAKEECSVTPCGQWKALDWSSCSVTCGQGRAT RQVMCVNYSDHVIDRSECDQDYIPETDQDCSMSPCPQRTPDSGLAQHPFQNEDYRPRSAS PSRTHVLGGNQWRTGPWGACSSTCAGGSQRRVVVCQDENGYTANDCVERIKPDEQRACES GPCPQWAYGNWGECTKLCGGGIRTRLVVCQRSNGERFPDLSCEILDKPPDREQCNTHACP HDAAWSTGPWSSCSVSCGRGHKQRNVYCMAKDGSHLESDYCKHLAKPHGHRKCRGGRCPK WKAGAWSQCSVSCGRGVQQRHVGCQIGTHKIARETECNPYTRPESERDCQGPRCPLYTWR AEEWQECTKTCGEGSRYRKVVCVDDNKNEVHGARCDVSKRPVDRESCSLQPCEYVWITGE WSECSVTCGKGYKQRLVSCSEIYTGKENYEYSYQTTINCPGTQPPSVHPCYLRDCPVSAT WRVGNWGSCSVSCGVGVMQRSVQCLTNEDQPSHLCHTDLKPEERKTCRNVYNCELPQNCK EVKRLKGASEDGEYFLMIRGKLLKIFCAGMHSDHPKEYVTLVHGDSENFSEVYGHRLHNP TECPYNGSRRDDCQCRKDYTAAGFSSFQKIRIDLTSMQIITTDLQFARTSEGHPVPFATA GDCYSAAKCPQGRFSINLYGTGLSLTESARWISQGNYAVSDIKKSPDGTRVVGKCGGYCG KCTPSSGTGLEVRVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
