Gene:
Gene Symbol:
Xylt1
Gene Name:
xylosyltransferase 1
Original Clone ID:
FLH270320.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083246
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2859nts
Open reading frame: 1 to 2859
ATGGTGGCGGCGCCGTGCGCCCGGAGGCTGGCGCGGCGCTCGCACTCGGCGCTGCTGGCG
GCGCTCATGGTGCTGCTGCTGCAGACGCTGGTGGTGTGGAATTTCAGCAGCCTGGACTCC
GGGGCTGGAGAGCAGCGGCGCGCGGGGGCAGCGGCGGGAGCAGCCGAGCAGCAGCAGCCC
GCGGCCCCGCGCCGGGAGCGCAGGGACCTGGCTGCCCATCTGCCCGCAGCCCGCGGAGGA
CCTGGAGGCCGAGCCGGCGGAGGAGGAGCACGGGGAGGCGGCCCCGGAGGAGCCCGCGCA
CAGCAGCCTGCCAGCCGCGGGGCACTAGCCTCCCGGGCGAGGGATCCACAACCAAGTCCA
CTGATCACCCTAGAGACCCAGGATGGCTACTTCTCTCACCGTCCCAAAGAGAAAGTTCGG
ACCGACAGCAACAATGAGAACTCAGTCCCCAAGGACTTTGAGAATGTGGATAACAGCAAC
TTCGCCCCCAGGACCCAGAAGCAGAAACACCAGCCAGAGCTGGCAAAGAAGCCCCCCAGC
AGGCAGAAAGAGCATTTGCAGAGAAAGCTGGATGCCCAGGACAAACGACAGGGCCAGTCA
GTCCTGGGGAAAGGCCCCAAGGAGGTGCTGCCTCCTCGGGAGAAAGCCACAGGCAACAGT
AGCCAAGGGAAGGATCTCTCAAGACACAGCCATGCCAGGAAGAGTGGTGGTGGTGGGTCC
CCCGAAACCAAGTCTGACCAGGCCCCCAAGTGTGATATCTCTGGCAAGGAGGCCATCTCA
GCACTGACCCGCGCTAAGTCCAAGCATTGTCGCCAGGAGATTGCAGAAACCTACTGTCGC
CACAAGCTGGGGCTGCTGATGCCAGAGAAGGTGGCTCGATTCTGTCCCCTAGAAGGCAAA
GCCAACAAGAATGTCCAGTGGGATGAGGATGCTGTGGAGTACATGCCTGCCAACCCAGTC
AGAATCGCCTTTGTCCTGGTGGTCCATGGCCGTGCCTCTCGACAGCTACAGCGCATGTTC
AAGGCCATCTACCACAAAGACCATTTCTACTATATCCATGTGGATAAGCGTTCCAATTAC
CTGCATCGGCAAGTGCTCCAGTTCTCCAGGCAGTACGACAATGTCCGAGTCACCTCCTGG
AGGATGGCCACCATTTGGGGTGGAGCCAGCCTCCTGTCCACCTACCTGCAGAGCATGCGG
GATCTACTAGAGATGACTGACTGGCCCTGGGACTTCTTCATCAACCTCAGTGCTGCTGAC
TACCCCATCAGGACAAATGACCAGCTGGTAGCATTTCTTTCCAGATATCGAGATATGAAC
TTCCTGAAGTCACATGGCCGGGACAATGCAAGGTTCATCCGGAAGCAGGGCCTGGACCGC
CTCTTCCTGGAGTGTGATACACACATGTGGCGCCTGGGGGACCGGCGGATCCCAGAGGGC
ATTGCTGTGGATGGTGGTTCTGATTGGTTCCTGCTAAACAGGAAGTTTGTAGAGTATGTG
GCATTCTCCACAGATGACCTGGTGACCAAGATGAAGCAGTTCTACTCTTACACCCTTCTC
CCTGCTGAGTCCTTTTTCCACACGGTTCTAGAGAACAGCCCCCACTGTGACACCATGGTG
GATAACAACCTGCGCATCACCAACTGGAACCGCAAGCTGGGCTGCAAGTGCCAGTACAAG
CATATCGTGGACTGGTGCGGCTGCTCTCCCAATGACTTCAAGCCTCAGGACTTCCATCGC
TTCCAGCAGACAGCCCGGCCCACCTTCTTTGCCCGAAAGTTCGAAGCCATAGTGAACCAG
GAGATCATTGGGCAGCTGGACTCTTACCTGTACGGGAACTATCCTGCGGGCACCCCGGGC
CTCCGCTCCTACTGGGAGAATGTCTACGATGAACCAGATGGCATCCACACCCTCAGCGAT
GTAGCCCTCACCCTGTACCATTCCTTCATCCGTCTGGGTCTTCGAAGAGCTGAGTCATCC
CTACACACGGATGGGGAGAACAGCTGCAGGTACTACCCAATGGGCCACCCAGCTTCTGTC
CATCTCTACTTCCTTGCTGACCGATTCCAGGGCTTTCTGATCAAGCATCACGTGACCAAC
CTTGCTGTGAGCAAACTGGAGACACTGGAGACATGGATGATGCCAAAGAAAGTCTTCAAG
GTCGCAAGTCCCCCCAGTGACTTTGGAAGGCTTCAGTTTTCTGAGGTTGGCACTGACTGG
GATGCCAAGGAGAGGCTGTTCCGGAACTTTGGTGGTCTTTTGGGGCCCATGGATGAGCCG
GTGGGGATGCAGAAATGGGGAAAGGGGCCCAATGTGACCGTGACTGTTATTTGGGTGGAT
CCTGTCAACGTCATTGCAGCCACCTATGATATCCTGATTGAGTCCACTGCGGAATTCACA
CACTACAAGCCCCCTTTGAATCTGCCTCTGAGGCCTGGGGTCTGGACAGTGAAGATTCTC
CATCACTGGGTGCCAGTGGCAGAGACCAAATTCCTTGTGGCACCTTTGACCTTTTCCAAC
AAGCAGCCCATCAAACCAGAGGAGGCTTTGAAGCTGCACAATGGGCCACCTCGCAGTGCC
TACATGGAGCAGAGTTTCCAGAGCCTGAACCCAGTCCTCAGCCTGCACATCAATCCTGCC
CAAGTCGAACAGGCCCGGAAGAATGCAGCCTTCACCGGGACAGCGCTAGAAGCCTGGCTG
GACTCGCTGGTGGGTGGGACTTGGACTGCCATGGACATCTGCACCACAGGCCCCACCGCC
TGCCCAGTCATGCAGACCTGCAGCCAAACAGCCTGGAGTTCCTTCAGCCCTGATCCCAAG
TCAGAGCTGGGTGCAGTCAAACCTGACGGACGGCTCAGG
Protein sequence predicted based on nucleotide sequence
MVAAPCARRLARRSHSALLAALMVLLLQTLVVWNFSSLDSGAGEQRRAGAAAGAAEQQQP AAPRRERRDLAAHLPAARGGPGGRAGGGGARGGGPGGARAQQPASRGALASRARDPQPSP LITLETQDGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPPS RQKEHLQRKLDAQDKRQGQSVLGKGPKEVLPPREKATGNSSQGKDLSRHSHARKSGGGGS PETKSDQAPKCDISGKEAISALTRAKSKHCRQEIAETYCRHKLGLLMPEKVARFCPLEGK ANKNVQWDEDAVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDKRSNY LHRQVLQFSRQYDNVRVTSWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAAD YPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDTHMWRLGDRRIPEG IAVDGGSDWFLLNRKFVEYVAFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMV DNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAIVNQ EIIGQLDSYLYGNYPAGTPGLRSYWENVYDEPDGIHTLSDVALTLYHSFIRLGLRRAESS LHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHVTNLAVSKLETLETWMMPKKVFK VASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVD PVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSN KQPIKPEEALKLHNGPPRSAYMEQSFQSLNPVLSLHINPAQVEQARKNAAFTGTALEAWL DSLVGGTWTAMDICTTGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
201
End on reference sequence
3062
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
