Gene:
Gene Symbol:
Taok1
Gene Name:
TAO kinase 1
Original Clone ID:
FLH270321.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083247
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3003nts
Open reading frame: 1 to 3003
ATGCCATCAACTAACAGAGCAGGCAGTCTAAAGGACCCTGAAATTGCAGAGCTCTTCTTC
AAAGAAGATCCGGAAAAGCTCTTCACAGATCTCAGAGAAATCGGCCATGGGAGCTTTGGA
GCAGTATATTTTGCACGAGATGTGCGTACTAATGAAGTGGTGGCCATCAAGAAAATGTCT
TATAGTGGAAAGCAGTCTACTGAGAAATGGCAGGATATTATTAAGGAAGTCAAGTTTCTA
CAAAGAATAAAACATCCCAACAGTATAGAATACAAAGGCTGCTATTTACGTGAACACACA
GCATGGCTTGTAATGGAATATTGTTTAGGATCTGCTTCAGATTTATTAGAAGTTCATAAA
AAGCCATTACAAGAAGTGGAAATAGCAGCAATTACACATGGTGCTCTCCAGGGACTAGCT
TATTTACATTCTCATACCATGATCCATAGAGATATCAAAGCAGGAAATATCCTTCTGACA
GAACCAGGCCAAGTGAAACTTGCTGACTTTGGATCTGCTTCCATGGCTTCCCCTGCCAAT
TCTTTTGTGGGAACACCATATTGGATGGCCCCAGAAGTAATTTTAGCCATGGATGAAGGA
CAGTATGATGGCAAAGTTGATGTATGGTCTCTTGGAATAACGTGTATTGAATTAGCCGAG
AGGAAGCCTCCTTTATTTAATATGAATGCAATGAGTGCCTTATATCACATAGCCCAAAAT
GAATCCCCTACACTACAATCTAATGAATGGTCTGATTATTTTCGAAACTTTGTAGATTCT
TGCCTCCAGAAAATCCCTCAAGATCGCCCTACATCAGAGGAACTTTTAAAGCACATGTTT
GTTCTTCGAGAGCGCCCTGAAACAGTGTTAATAGATCTTATTCAAAGGACAAAGGATGCA
GTAAGAGAGCTGGACAATCTGCAGTATCGAAAGATGAAGAAACTCCTTTTCCAGGAGGCA
CATAATGGGCCAGCGGTAGAAGCACAGGAAGAAGAGGAGGAGCAAGATCATGGTGTTGGC
CGAACAGGAACAGTGAATAGTGTTGGAAGCAATCAGTCTATCCCTAGTATGTCTATCAGT
GCCAGCAGTCAAAGCAGCAGTGTTAATAGTCTTCCAGATGCATCAGATGACAAGAGTGAG
CTAGACATGATGGAGGGAGACCATACAGTGATGTCTAACAGTTCTGTCATCCACTTAAAA
CCTGAGGAGGAAAATTACCAGGAAGAAGGAGATCCTAGAACAAGAGCATCAGACCCACAG
TCTCCCCCTCAGGTGTCTCGTCACAAGTCACATTATCGTAATAGAGAACACTTTGCAACC
ATACGAACAGCATCACTGGTTACAAGACAGATGCAAGAACATGAGCAGGACTCTGAACTT
AGAGAACAGATGTCTGGTTATAAGCGGATGAGGCGACAGCATCAAAAGCAGCTGATGACG
CTGGAAAATAAACTGAAGGCAGAGATGGACGAACATCGGCTCAGATTAGACAAAGATCTT
GAAACTCAGCGTAACAATTTCGCTGCAGAAATGGAGAAACTTATTAAGAAACACCAAGCT
GCTATGGAAAAAGAGGCTAAAGTGATGGCCAATGAGGAGAAAAAATTCCAGCAACACATT
CAGGCTCAACAGAAAAAAGAACTGAATAGCTTTTTGGAGTCTCAAAAAAGAGAATATAAA
CTTCGCAAAGAGCAGCTTAAGGAGGAGCTGAATGAAAACCAGAGCACACCTAAAAAAGAA
AAGCAGGAATGGCTTTCAAAGCAGAAGGAGAATATACAGCATTTTCAGGCAGAAGAAGAA
GCTAATCTTCTTCGACGTCAAAGGCAGTATCTAGAGCTAGAATGTCGTCGCTTCAAAAGA
AGAATGTTACTTGGGCGACATAACTTGGAACAGGACCTTGTCAGGGAGGAGTTAAACAAA
AGGCAGACTCAAAAGGACTTGGAACATGCAATGCTATTGCGACAGCATGAATCAATGCAA
GAACTGGAGTTTCGCCATCTCAACACTATTCAGAAGATGCGCTGTGAGTTGATCAGACTG
CAGCATCAAACTGAGCTCACTAACCAGCTAGAGTACAATAAGAGAAGGGAACGGGAACTG
AGGCGAAAACATGTCATGGAAGTTCGACAACAACCTAAGAGTCTGAAGTCTAAAGAACTC
CAAATAAAAAAGCAGTTTCAGGATACCTGCAAAATTCAAACCAGACAGTACAAAGCATTA
AGGAATCACCTACTGGAGACTACACCAAAGAGTGAGCACAAAGCTGTTTTGAAAAGACTC
AAGGAGGAACAGACTCGAAAGTTAGCCATCTTGGCTGAGCAGTATGATCATAGCATTAAT
GAAATGCTCTCCACACAAGCTCTGCGTTTGGATGAAGCACAGGAAGCAGAATGCCAGGTT
CTGAAGATGCAGCTGCAGCAGGAACTGGAGCTGCTGAATGCTTATCAGAGCAAAATAAAG
ATGCAAGCTGAGGCCCAACATGATCGAGAGCTTCGAGAGCTGGAACAGAGGGTCTCCCTT
CGCAGAGCGCTCTTAGAACAAAAGATTGAAGAAGAGATGTTGGCTTTGCAGAATGAACGC
ACAGAACGAATACGTAGCCTGCTCGAGCGTCAAGCCAGAGAAATTGAAGCATTTGACTCT
GAAAGCATGAGATTAGGTTTTAGTAACATGGTCCTTTCTAATCTCTCCCCTGAGGCATTC
AGCCACAGCTACCCAGGAGCTTCTAGTTGGTCTCACAATCCTACTGGGGGTCCAGGACCT
CACTGGGGTCATCCCATGGGTGGCACACCACAAGCTTGGGGTCATCCGATGCAAGGTGGA
CCCCAACCATGGGGTCATCCCTCAGGGCCAATGCAAGGGGTACCTCGAGGTAGCAGTATG
GGAGTCCGCAATAGCCCCCAGGCTTTGAGGCGGACAGCTTCTGGGGGACGGACGGAACAG
GGCATGAGCAGAAGCACGAGTGTCACTTCACAAATATCCAATGGGTCACACATGTCTTAC
ACA
Protein sequence predicted based on nucleotide sequence
MPSTNRAGSLKDPEIAELFFKEDPEKLFTDLREIGHGSFGAVYFARDVRTNEVVAIKKMS YSGKQSTEKWQDIIKEVKFLQRIKHPNSIEYKGCYLREHTAWLVMEYCLGSASDLLEVHK KPLQEVEIAAITHGALQGLAYLHSHTMIHRDIKAGNILLTEPGQVKLADFGSASMASPAN SFVGTPYWMAPEVILAMDEGQYDGKVDVWSLGITCIELAERKPPLFNMNAMSALYHIAQN ESPTLQSNEWSDYFRNFVDSCLQKIPQDRPTSEELLKHMFVLRERPETVLIDLIQRTKDA VRELDNLQYRKMKKLLFQEAHNGPAVEAQEEEEEQDHGVGRTGTVNSVGSNQSIPSMSIS ASSQSSSVNSLPDASDDKSELDMMEGDHTVMSNSSVIHLKPEEENYQEEGDPRTRASDPQ SPPQVSRHKSHYRNREHFATIRTASLVTRQMQEHEQDSELREQMSGYKRMRRQHQKQLMT LENKLKAEMDEHRLRLDKDLETQRNNFAAEMEKLIKKHQAAMEKEAKVMANEEKKFQQHI QAQQKKELNSFLESQKREYKLRKEQLKEELNENQSTPKKEKQEWLSKQKENIQHFQAEEE ANLLRRQRQYLELECRRFKRRMLLGRHNLEQDLVREELNKRQTQKDLEHAMLLRQHESMQ ELEFRHLNTIQKMRCELIRLQHQTELTNQLEYNKRRERELRRKHVMEVRQQPKSLKSKEL QIKKQFQDTCKIQTRQYKALRNHLLETTPKSEHKAVLKRLKEEQTRKLAILAEQYDHSIN EMLSTQALRLDEAQEAECQVLKMQLQQELELLNAYQSKIKMQAEAQHDRELRELEQRVSL RRALLEQKIEEEMLALQNERTERIRSLLERQAREIEAFDSESMRLGFSNMVLSNLSPEAF SHSYPGASSWSHNPTGGPGPHWGHPMGGTPQAWGHPMQGGPQPWGHPSGPMQGVPRGSSM GVRNSPQALRRTASGGRTEQGMSRSTSVTSQISNGSHMSYT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3704
Start on reference sequence
699
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
