Gene:
Gene Symbol:
4631416L12Rik
Gene Name:
RIKEN cDNA 4631416L12 gene
Original Clone ID:
FLH270325.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083251
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2607nts
Open reading frame: 1 to 2607
ATGGACGGCGAAAGCGAGGTGGATTTTTCCAGCAACGGCGTCACCCCTTTGTGGCGGAGA
CGGTCCACCCCTCCGCTCCAGTTGCTCAGCCGGAGCAAGCCGCGGCCACAGTCGTACCAG
AGCCCCAGCGGGCTGCTGATCACCGACTTCCCTGTGGAGGAGCTGGCTGCCCTTCCCACG
CCGCAGACTCCGGTCCGCGTACCCGCCGACCCGGACGACAGGACGGTGTCCAGGAGCCCC
CAGCTTCTGAGCGCGCATCGGAGGCCGGTGACCAATGGTCGCACGGTGTCCCCGGACTAC
AGAGCTGCTTCTCCTCGACTCCGACGGCCCAAGTCACCCCTGGTTTCTTACGCGGCGTCG
GGCGGCTCCCCGAAGTCCACTTCTAATGGCACCGTGGCCTCGCCCGCGGCGCTGTTGTCG
CGCGCCTCCCGGACTCCGGACACCCCGGCCTCTTGCGCCACCGATGAGGACTGCACCGGG
GTGACTGCCAGTAAGGTATCTTCGCCCATTGCCAATGGCCTTGCCCATAAAACCGACTCA
TCTGGCCCAGTATCACAAACTGGTCAGCCAGCTGGGGACTTGGAGCTGGCCCCCTCGAGA
CTCTCTCCTGCTCTCCCACACAAGTCTTCAGAACAAAAACTGCCACTCCAAAGGCTGCCC
TCCCAAGGGAATGAGCTCCCAAACCCTTCCGTGGTTTTGAGTACCAACAGCCCGGCGGCC
CTCAAGATGGGAAAGCAGCAGATTATCCCAAAAAGCCTTGCCTCTGAAATTAAACTGAGT
AAATCCAATAGTCAGAACGTGGAGTCCCATAGGAGGCTTCTCAAGGTGCGCAGCATGGTG
GAGGGACTGGGAGCACCCTTGGGCCACGAAGGGGAGGAAGGCGAAGTCGACAACGACATG
GACAGTCCCGGCTCCCTTCGGCGAGGCTTGAGGTCCACGTCTTACAGAAGGGCAGTGGTC
AGCGGCTTTGATTTTGACAGTCCCACCAGCTCAAAGAAGAAGAACAGAATGTCCCAGCCT
GTCCTGAAGGCCGTAATGGAAGACAAGGAGAAGTTCTCTAGCCTGGGAAGGATAAAGAAA
AAAATACTGAAAGGACAAGGAACATTTGATGGGGAAGAAAATGCAGTCCTGTATCAAAAC
TACAAAGAAAAGGCCCTTGATATCGACTCTGATGAGGAACCAGAGGCCAAGGAGCAGAAG
GCAGAGGAGAGAATTGTGATTCACCACAAGCCCCTGCGCTCCACGTGGAGCCAGCTCTCC
GCGGTGAAAAGAAATGGGCTGTCTCAGACAGTGAGCCAGGAGGAAAGGAAGAGACAAGAG
GCAATTTTTGAAGTCATATCATCAGAGCATTCTTATTTACTCAGCTTGGAGATCTTGATA
CGGATGTTTAAAAACTCTAAGGAACTGACTGATACAATGACTAAGACCGAGAGGCATCAC
CTTTTCTCCAACATTACCGATGTCTGGGAGGCCAGCAAAAAATTCTTCACAGAGTTGGAA
GCAAGACATCAGAATAACATCTTCATAGAGGATATCAGTGACATTGTAGAAAAGCACACA
GCATCCACCTTTGACCCCTACGTGAAATACTGCACCAATGAAGTCTACCAACAGCGCACG
CTGCAGAAGTTGCTAGCAACCAACCCGTCCTTTAAGGAAGTGTTGTCGAGGATCGAGTCC
CATGAAGACTGCAGGAACTTACCTATGATCTCTTTTCTCATTCTCCCCATGCAGAGAGTG
ACACGCCTTCCCCTGCTGATGGATACTATCTGTCAAAAAACACCCAAGGACTCTCCAAAG
TATGAAGTCTGCAAAAGGGCCTTGAAGGAAGTGAGCAAGTTGGTCCGACTGTGCAATGAA
GGAGCACGGAAGATGGAGCGGACAGAGATGATGTACACGATTAATTCCCAGCTGGAATTT
AAAATCAAGCCTTTCCCCCTGGTCTCCTCTTCCCGGTGGTTGGTGAAAAGAGGGGAGTTG
ACAGCCTATGTGGAAGACACAGTGCTGTTCTCCAAGAGGATGTCCAAACAGCAGGTCTAC
TTCTTCCTGTTCAATGATGTGCTTATTATCACGAAGAAGAAGAGTGAAGAAAGCTACAAT
GTCAATGATTACTCCTTAAGGGACCAGCTGTTGGTGGAATCCTGTGACAATGAAGAGCTT
AATTCCTCTCCAGGGAAGAACCCTTCCACGATGCTTTACTCAAGACAGAGCTCTGCCACT
CACCTCTTTACCCTGACAGTCCTCAGCAACCATGCCAGTGAGAAGGTGGACATGCTACTA
GGCGCTGAGACCCAGAGTGAGCGAGCCCGCTGGATCACAGCCCTGGGACACAGCAGTGGG
AAACAGCCACCAGACAGAACCACCCTGACCCAGGTGGAAATCATTAGGTCTTTTACTGCC
AAACAGCCTGATGAACTTTCCCTGCAGGTGGCAGACGTGGTCCTCATTTATCAGCGTGTT
GGCGATGGCTGGTATGAAGGGGAACGACTTCGAGATGGAGAGAGGGGCTGGTTTCCAATG
GAATGTGCCAAGGAGATAACATGTCAAGCTACTATTGATAAGAATGTGGAGAGGATGGGA
CGATTGCTAGGACTGGAAACCAACGTG
Protein sequence predicted based on nucleotide sequence
MDGESEVDFSSNGVTPLWRRRSTPPLQLLSRSKPRPQSYQSPSGLLITDFPVEELAALPT PQTPVRVPADPDDRTVSRSPQLLSAHRRPVTNGRTVSPDYRAASPRLRRPKSPLVSYAAS GGSPKSTSNGTVASPAALLSRASRTPDTPASCATDEDCTGVTASKVSSPIANGLAHKTDS SGPVSQTGQPAGDLELAPSRLSPALPHKSSEQKLPLQRLPSQGNELPNPSVVLSTNSPAA LKMGKQQIIPKSLASEIKLSKSNSQNVESHRRLLKVRSMVEGLGAPLGHEGEEGEVDNDM DSPGSLRRGLRSTSYRRAVVSGFDFDSPTSSKKKNRMSQPVLKAVMEDKEKFSSLGRIKK KILKGQGTFDGEENAVLYQNYKEKALDIDSDEEPEAKEQKAEERIVIHHKPLRSTWSQLS AVKRNGLSQTVSQEERKRQEAIFEVISSEHSYLLSLEILIRMFKNSKELTDTMTKTERHH LFSNITDVWEASKKFFTELEARHQNNIFIEDISDIVEKHTASTFDPYVKYCTNEVYQQRT LQKLLATNPSFKEVLSRIESHEDCRNLPMISFLILPMQRVTRLPLLMDTICQKTPKDSPK YEVCKRALKEVSKLVRLCNEGARKMERTEMMYTINSQLEFKIKPFPLVSSSRWLVKRGEL TAYVEDTVLFSKRMSKQQVYFFLFNDVLIITKKKSEESYNVNDYSLRDQLLVESCDNEEL NSSPGKNPSTMLYSRQSSATHLFTLTVLSNHASEKVDMLLGAETQSERARWITALGHSSG KQPPDRTTLTQVEIIRSFTAKQPDELSLQVADVVLIYQRVGDGWYEGERLRDGERGWFPM ECAKEITCQATIDKNVERMGRLLGLETNV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3330
Start on reference sequence
721
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
