Gene:
Gene Symbol:
Ptprt
Gene Name:
protein tyrosine phosphatase, receptor type, T
Original Clone ID:
FLH270342.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083268
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4305nts
Open reading frame: 1 to 4305
ATGGGGAGCCTTGGCGGGCTCGCCCTCTGCCTGCTCCGGCTCCTGCTCCTGGGGCTGCAG
CGGCCGCCGTTGCCCGGCGCCGGAGCGCAGAGCGCCGCAGGTGGCTGTTCTTTTGACGAA
CATTACAGCAACTGCGGGTATAGCGTGGCTCTGGGAACCAATGGGTTTACCTGGGAGCAG
ATTAACACATGGGAGAAGCCAATGCTGGACCCAGCTGTGCCCACAGGGTCCTTCATGATG
GTGAACAGCTCTGGAAGGGCTTCAGGCCAGAAAGCCCATCTTCTTCTGCCAACCTTGAAG
GAGAATGACACTCACTGCATTGACTTCCATTACTACTTCTCCAGTCGAGATCGCTCCAGC
CCGGGAGCCTTGAATGTCTACGTGAAGGTGAATGGTGGACCCCAAGGGAACCCTGTCTGG
AACGTATCTGGCGTCGTCACTGAGGGCTGGGTGAAGGCAGAGCTTGCCATCAGCACCTTC
TGGCCTCATTTCTATCAGGTGATATTTGAATCCGTCTCTTTGAAAGGTCATCCTGGTTAC
ATCGCTGTGGACGAAGTTCGGGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTCCTG
CGACTCCAAAACGTTGAGGTGAATGTGGGGCAGAACGCCACGTTTCAGTGCATTGCTGGT
GGAAAGTGGTCCCAGCATGACAAACTTTGGCTCCAGCAATGGAATGGCAGAGACACAGCC
CTCATGGTCACCCGGGTGGTCAACCACAGACGCTTCTCAGCCACAGTGAGTGTGGCAGAC
ACCTCTCAACGCAGTATCTCCAAGTATCGCTGCGTGATCCGCTCAGATGGTGGGTCTGGT
GTGTCCAACTATGCAGAGCTGATTGTGAAAGAGCCTCCCACGCCCATTGCTCCCCCGGAA
CTACTGGCCGTGGGTGCCACCTACCTGTGGATTAAACCAAATGCCAACTCCATTATTGGG
GATGGCCCCATCATACTGAAGGAGGTAGAATACCGCACAACCACAGGAACCTGGGCCGAG
ACCCACATCGTGGACTCTCCCAACTACAAGCTCTGGCATCTGGACCCTGATGTGGAGTAT
GAGATCCGGGTGCTGCTCACACGACCAGGAGAGGGGGGCACAGGACCACCAGGACCACCC
CTAACTACCAGGACCAAGTGTGCCGATCCCGTGCATGGCCCGCAGAATGTGGAGATTGTG
GACATTCGGGCTCGGCAGCTGACCCTGCAGTGGGAACCCTTTGGCTATGCAGTGACCCGC
TGCCACAGCTACAACCTCACAGTGCAGTACCAGTACGTGTTCAACCAGCAGCAGTATGAA
GCTGAAGAGGTGATCCAGACATCTTCCCACTATACCCTTCGGGGTCTACGGCCCTTCATG
ACCATCAGACTGCGGCTGCTACTGTCCAACCCTGAGGGCCGGATGGAGAGTGAGGAGTTG
GTGGTACAGACAGAAGAGGATGTTCCAGGAGCTGTTCCTCTCGAGTCCATCCAAGGGGGT
CCCTTTGAGGAGAAGATCTACATCCAGTGGAAACCTCCCAATGAGACCAATGGGGTCATC
ACACTCTATGAGATTAACTACAAGGCTGTGGGCTCACTGGATCCAAGTGCTGACCTCTCC
AGCCAGAGGGGAAAGGTGTTCAAACTCCGGAATGAAACCCACCACCTCTTTGTGGGTCTG
TACCCTGGGACTACCTACTCTTTCACCATCAAGGCCAGCACAGCCAAGGGCTTTGGACCC
CCAGTCACTACTCGGATTGCCACCAAGATTTCAGCTCCATCAATGCCTGAATATGACGCA
GATACCCCACTCAATGAAACAGATACAACCATCACGGTGATGCTCAAACCCGCCCAGTCC
AGGGGAGCCCCAGTCAGTGTTTACCAGCTGGTTGTCAAGGAGGAGCGACTCCAGAAGTCT
CGGAGAGCAGCTGACATCATTGAGTGTTTCTCAGTACCTGTGAGCTACCGGAACGCCTCC
AACCTCGATTCGCTACACTACTTTGCTGCTGAGCTGAAGCCCTCCAACCTGCCTGTCACC
CAGCCATTTACAGTGGGAGACAACAAGACCTACAATGGCTACTGGAACCCTCCTCTCTCC
CCATTGAAGAGCTACAGCATCTACTTCCAGGCCCTCAGCAAAGCAAATGGAGAGACAAAA
ATCAACTGTGTGCGTCTGGCTACAAAAGGTGCTTCCACTCAGAATTCTAACACTGTGGAG
CCGGAGAAGCAGGTCGACAACACGGTGAAGATGGCTGGCGTGATTGCTGGCCTCCTCATG
TTCATCATCATTCTCCTGGGGGTGATGCTGACCATCAAAAGGAGGAAGCTGGCTAAGAAG
CAGAAGGAGACCCAGAGTGGAGCCCAGAGGGAGATGGGTCCTGTGGCCTCGACTGACAAG
CCTACCGCCAAGCTCGGCACCAACCGCAATGATGAAGGCTTCTCCTCCAGCTCTCAGGAT
GTTAATGGATTCACAGATGGCAGCCGTGGGGAGCTGTCTCAGCCTACCCTCACCATTCAG
ACTCATCCCTACCGGACTTGTGACCCTGTAGAGATGAGCTATCCCCGGGACCAGTTCCAG
CCTGCCATCCGGGTGGCGGACCTGCTTCAACACATCACCCAGATGAAGAGAGGCCAGGGC
TACGGGTTCAAGGAGGAATACGAGGCCTTACCAGAAGGACAGACAGCTTCGTGGGACACA
GCCAAGGAAGATGAAAACCGCAATAAGAATCGATACGGAAACATCATATCTTATGACCAC
TCTCGAGTAAGGCTGTTGGTGCTGGATGGAGACCCTCACTCAGACTACATCAATGCCAAC
TACATTGACGGGTACCACCGACCCCGGCACTACATTGCAACCCAAGGTCCAATGCAAGAG
ACGGTGAAGGACTTTTGGAGAATGATCTGGCAGGAAAACTCTGCCAGCATCGTCATGGTC
ACAAACCTTGTGGAAGTGGGCAGGGTGAAGTGTGTCCGATACTGGCCAGATGACACAGAG
GTCTATGGAGACATTAAAGTCACCCTAATAGAAACAGAGCCCCTGGCAGAATACGTCATC
CGCACCTTCACAGTCCAGAAGAAAGGCTACCATGAGATCCGGGAGCTCCGCCTCTTCCAC
TTCACCAGCTGGCCTGACCACGGTGTTCCCTGCTATGCCACTGGCCTTCTGGGCTTTGTC
CGCCAGGTCAAGTTTCTCAATCCCCCAGAAGCTGGGCCCATAGTGGTCCATTGCAGTGCT
GGAGCCGGGAGGACTGGCTGCTTCATTGCGATTGACACCATGCTCGACATGGCTGAGAAT
GAAGGGGTTGTGGACATCTTCAACTGTGTGCGTGAGCTCCGGGCACAGAGGGTCAACCTG
GTGCAGACAGAGGAGCAGTACGTGTTTGTGCACGATGCCATCCTGGAAGCATGCCTCTGC
GGCAATACTGCCATCCCAGTGTGTGAGTTCCGCTCTCTCTACTACAACATCAGCAGGCTG
GACCCGCAGACCAACTCCAGCCAGATCAAAGACGAGTTTCAGACACTCAACATTGTGACA
CCTCGAGTGCGGCCTGAAGACTGCAGCATTGGGCTCTTACCCCGGAACCATGATAAGAAT
CGGAGCATGGATGTCCTGCCTCTGGACCGCTGTCTACCCTTCCTCATCTCAGTAGATGGA
GAGTCCAGCAACTACATCAATGCAGCACTGATGGATAGCCACAAGCAGCCTGCCGCCTTC
GTGGTCACCCAGCATCCTCTACCCAACACGGTGGCAGACTTCTGGAGGCTGGTGTTTGAT
TATAATTGTTCATCTGTGGTGATGCTGAACGAGATGGATACTGCTCAGCTCTGTATGCAG
TACTGGCCTGAGAAGACCTCCGGGTGTTATGGTCCCATCCAGGTGGAGTTTGTCTCTGCA
GACATCGATGAGGACATCATCCACAGAATCTTCCGGATCTGTAACATGGCTCGGCCACAG
GATGGTTATCGTATTGTCCAGCACCTCCAGTACATCGGCTGGCCTGCATACCGGGACACG
CCCCCCTCTAAGCGCTCTCTGCTCAAAGTGGTCCGACGGCTGGAGAAATGGCAGGAGCAA
TACGACGGAAGAGAGGGGCGCACTGTGGTCCACTGCCTAAATGGGGGAGGCCGCAGTGGA
ACCTTCTGTGCTATCTGCAGTGTGTGTGAGATGATCCAGCAGCAGAACATTATTGACGTG
TTCCACATTGTGAAAACCCTCCGCAACAACAAGTCCAACATGGTGGAGACGCTGGAACAG
TATAAATTTGTATATGAGGTGGCACTGGAATATTTAAGCTCCTTT
Protein sequence predicted based on nucleotide sequence
MGSLGGLALCLLRLLLLGLQRPPLPGAGAQSAAGGCSFDEHYSNCGYSVALGTNGFTWEQ INTWEKPMLDPAVPTGSFMMVNSSGRASGQKAHLLLPTLKENDTHCIDFHYYFSSRDRSS PGALNVYVKVNGGPQGNPVWNVSGVVTEGWVKAELAISTFWPHFYQVIFESVSLKGHPGY IAVDEVRVLAHPCRKAPHFLRLQNVEVNVGQNATFQCIAGGKWSQHDKLWLQQWNGRDTA LMVTRVVNHRRFSATVSVADTSQRSISKYRCVIRSDGGSGVSNYAELIVKEPPTPIAPPE LLAVGATYLWIKPNANSIIGDGPIILKEVEYRTTTGTWAETHIVDSPNYKLWHLDPDVEY EIRVLLTRPGEGGTGPPGPPLTTRTKCADPVHGPQNVEIVDIRARQLTLQWEPFGYAVTR CHSYNLTVQYQYVFNQQQYEAEEVIQTSSHYTLRGLRPFMTIRLRLLLSNPEGRMESEEL VVQTEEDVPGAVPLESIQGGPFEEKIYIQWKPPNETNGVITLYEINYKAVGSLDPSADLS SQRGKVFKLRNETHHLFVGLYPGTTYSFTIKASTAKGFGPPVTTRIATKISAPSMPEYDA DTPLNETDTTITVMLKPAQSRGAPVSVYQLVVKEERLQKSRRAADIIECFSVPVSYRNAS NLDSLHYFAAELKPSNLPVTQPFTVGDNKTYNGYWNPPLSPLKSYSIYFQALSKANGETK INCVRLATKGASTQNSNTVEPEKQVDNTVKMAGVIAGLLMFIIILLGVMLTIKRRKLAKK QKETQSGAQREMGPVASTDKPTAKLGTNRNDEGFSSSSQDVNGFTDGSRGELSQPTLTIQ THPYRTCDPVEMSYPRDQFQPAIRVADLLQHITQMKRGQGYGFKEEYEALPEGQTASWDT AKEDENRNKNRYGNIISYDHSRVRLLVLDGDPHSDYINANYIDGYHRPRHYIATQGPMQE TVKDFWRMIWQENSASIVMVTNLVEVGRVKCVRYWPDDTEVYGDIKVTLIETEPLAEYVI RTFTVQKKGYHEIRELRLFHFTSWPDHGVPCYATGLLGFVRQVKFLNPPEAGPIVVHCSA GAGRTGCFIAIDTMLDMAENEGVVDIFNCVRELRAQRVNLVQTEEQYVFVHDAILEACLC GNTAIPVCEFRSLYYNISRLDPQTNSSQIKDEFQTLNIVTPRVRPEDCSIGLLPRNHDKN RSMDVLPLDRCLPFLISVDGESSNYINAALMDSHKQPAAFVVTQHPLPNTVADFWRLVFD YNCSSVVMLNEMDTAQLCMQYWPEKTSGCYGPIQVEFVSADIDEDIIHRIFRICNMARPQ DGYRIVQHLQYIGWPAYRDTPPSKRSLLKVVRRLEKWQEQYDGREGRTVVHCLNGGGRSG TFCAICSVCEMIQQQNIIDVFHIVKTLRNNKSNMVETLEQYKFVYEVALEYLSSF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
249
End on reference sequence
4556
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
