Gene:
Gene Symbol:
Tle2
Gene Name:
transducin-like enhancer of split 2, homolog of Drosophila E(spl)
Original Clone ID:
FLH270350.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083275
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2301nts
Open reading frame: 1 to 2301
ATGTACCCTCAGGGAAGGCACCCGACCCCGCTGCAGTCTGGCCAGCCTTTCAAGTTCTCA
GTACTGGAAATCTGTGACCGGATCAAAGAGGAATTCCAGTTTCTTCAAGCTCAGTACCAC
AGCCTCAAGCTAGAATGTGAGAAGCTGGCCAGCGAGAAGACAGAAATGCAAAGGCATTAT
GTGATGGCTGCACCCCATCAGTGTCCCCAGGGTGGCACCAGCTATCCACACTGGCCAAGA
CTGTCTCCTTTGCAGTACTACGAGATGTCCTACGGACTCAACATTGAGATGCATAAACAG
GCTGAGATTGTGAAACGCCTCAGTGCGATCTGTGCCCAGATGGTCCCGTTCCTCACTCAG
GAGCATCAGCAGCAGGTGCTCCAGGCTGTGGACCGAGCCAAGCAGGTGACCGTGGGGGAA
CTGAACAGCCTCCTGGGGCAGCAGAATCAGCTCCAGCCGCTGTCCCACGCACCCCCCGTG
CCTCTCACCCCGCGCCCAGCCGGCCTGGTGGGTGCCGGGGCCACTGGGCTGCTGGCCCTA
TCTGGGGCACTGGCTGCGCAGGCCCAGCTGGTGGCTGCCGTAAAGGAAGACCGTGTGGGT
GTGGACGCCGAGGGGTCCAGAGTGGACAGAGCTGCCAGCAGGAGTTCTTCCCCGTCTCCC
CCTGAGAGTCTGGTGGAAGAGGACCATCCCAGCAGCCGAGGCGGCAGTGGGAAACAGCAG
AGAGCTGAAGACAAGGATCTGTCAGGGCCTTATGACAGTGAGGAAGACAAGAGTGACTAT
AACCTGGTAGTGGATGAGGACCAACCGTCAGAGCCCCCCAGCCCTGTGACCACCCCTTGT
GGGAAGGCGCCCCTCTGCATTCCTGCCCGCAGGGACCTCACAGACAGTCCAGCCTCCTTG
GCCTCCAGTTTGGGCTCACCACTCCCCAGAAGCAAAGACATAGCCCTGAACGATCTTCCC
ACAGGCACTCCTGCCTCCAGGTCATGTGGTACCTCTCCACCCCAGGACTCGTCCACCCCG
GGGCCCAGCTCAGCCAGTCACCTCTGCCAGCTGGCGGCTCAGCCGGCAGCACCCACAGAC
AGCATCGCCCTGAGGAGTCCCCTGACCTTGTCCAGCCCTTTCACCTCATCCTTCAGCCTG
GGCTCCCACAGCACCCTCAATGGGGACCTCTCCATGCCTGGCTCCTATGTCGGCCTCCAC
CTGTCCCCCCAGGTCAGCAGCTCTGTCGTGTATGGACGCTCACCTCTGCAGATGGCATTT
GAATCGCACCCCCATCTCCGAGGCTCGTCTGTCTCCTTGCCTGGCATCCCTGTGGCTAAG
CCGGCTTACTCCTTCCACGTGTCTGCGGATGGCCAGATGCAGCCTGTGCCCTTCCCGTCT
GATGCTCTGGTAGGCACAGGCATCCCTCGCCACGCAAGGCAGCTACACACGCTGGCCCAC
GGTGAGGTGGTGTGTGCCGTCACCATCAGCAGCTCCACACAGCACGTGTACACAGGCGGC
AAAGGATGCGTGAAGGTGTGGGACGTGGGCCAGCCGGGTAGCAAGACCCCTGTGGCACAG
CTGGATTGCCTGAACCGAGACAACTACATCCGCTCCTGCAAGCTGCTGCCCGACGGGCAG
AGCCTGATTGTAGGTGGCGAGGCCAGTACCCTGTCCATTTGGGACCTGGCAGCACCCACA
CCACGGATCAAAGCAGAGCTGACTTCGTCTGCCCCGGCCTGCTATGCTCTGGCCGTCAGT
CCGGACGCCAAGGTCTGCTTCTCCTGCTGCAGCGACGGTAACATCGTGGTCTGGGACCTG
CAGAACCAGGCCATGGTCAGACAGTTCCAGGGCCACACGGACGGGGCCAGCTGCATCGAC
ATATCAGACTACGGGACCCGGCTGTGGACTGGGGGCCTGGACAACACTGTGCGCTGCTGG
GACCTGCGCGAGGGCCGCCAGCTTCAGCAGCATGACTTCAGTTCCCAGATTTTCTCATTG
GGCCACTGTCCCAATCAGGACTGGTTGGCTGTGGGGATGGAGAGCAGCCACGTGGAGGTC
CTGCATGTGCGCAAGCCTGAGAAGTACCAGCTCCGCCTCCATGAGAGCTGCGTGCTGTCA
CTCAAGTTCGCTTCCTGTGGACGCTGGTTTGTGAGCACAGGCAAGGACAACCTGCTCAAT
GCCTGGAGGACACCCTATGGGGCCAGCATTTTTCAGTCCAAAGAATCATCTTCTGTACTG
AGCTGCGACATCTCCAGGAATAATAAGTACATCGTGACAGGCTCAGGGGACAAGAAGGCC
ACTGTGTATGAGGTGGTGTAC
Protein sequence predicted based on nucleotide sequence
MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHY VMAAPHQCPQGGTSYPHWPRLSPLQYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQ EHQQQVLQAVDRAKQVTVGELNSLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLAL SGALAAQAQLVAAVKEDRVGVDAEGSRVDRAASRSSSPSPPESLVEEDHPSSRGGSGKQQ RAEDKDLSGPYDSEEDKSDYNLVVDEDQPSEPPSPVTTPCGKAPLCIPARRDLTDSPASL ASSLGSPLPRSKDIALNDLPTGTPASRSCGTSPPQDSSTPGPSSASHLCQLAAQPAAPTD SIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVGLHLSPQVSSSVVYGRSPLQMAF ESHPHLRGSSVSLPGIPVAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAH GEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQ SLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDL QNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSL GHCPNQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLN AWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
137
End on reference sequence
2440
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
