Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
antibiotic resistance: Kan
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00832970
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5265nts
Open reading frame: 1 to 5265
ATGCTCTCAGCAACCCCCCTGTATGGGAACGTTCACAGCTGGATGAACAGCGAGAGGGTC
CGCATGTGTGGGGCGAGCGAAGACAGGAAAATCCTTGTAAATGATGGTGACGCTTCAAAA
GCCAGACTGGAACTGAGGGAAGAGAATCCCTTGAACCACAACGTGGTGGATGCGAGCACG
GCCCATAGGATCGATGGCCTGGCAGCACTGAGCATGGACCGCACTGGCCTGATCCGGGAA
GGGCTGCGGGTCCCGGGAAACATCGTCTATTCTAGCTTGTGTGGACTGGGCTCAGAGAAA
GGTCGGGAGGCTGCCACAAGCACTCTAGGTGGCCTTGGGTTTTCTTCGGAAAGAAATCCA
GAGATGCAGTTCAAACCGAATACACCCGAGACAGTGGAGGCTTCTGCCGTCTCTGGAAAA
CCCCCAAATGGCTTCAGTGCTATATACAAAACACCGCCTGGAATACAAAAAAGTGCTGTA
GCCACAGCAGAAGCGCTGGGCTTGGACAGGCCTGCCAGCGACAAACAGAGCCCTCTCAAC
ATCAATGGTGCTAGTTATCTGCGGCTGCCCTGGGTCAATCCTTACATGGAGGGTGCCACG
CCAGCCATCTACCCTTTCCTCGACTCGCCAAATAAGTATTCACTGAACATGTACAAGGCC
TTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGCTGTATTCTCCAGTCTGCACCAAT
GGGGAGCGCTTTCTCTACCTGCCGCCACCTCACTACGTCGGTCCCCACATCCCATCGTCC
TTGGCATCACCCATGAGGCTCTCGACACCTTCGGCCTCCCCAGCCATCCCGCCTCTCGTC
CATTGCGCAGACAAAAGCCTCCCGTGGAAGATGGGCGTCAGCCCTGGGAATCCTGTTGAT
TCCCACGCCTATCCTCACATCCAGAACAGTAAGCAGCCCAGGGTTCCCTCTGCCAAGGCG
GTCACCAGTGGCCTGCCGGGGGACACAGCTCTCCTGTTGCCCCCCTCGCCTCGGCCGTCA
CCCCGAGTCCACCTTCCCACCCAGCCTGCTGCAGACACCTACTCGGAGTTCCACAAGCAC
TATGCCAGGATCTCCACCTCTCCTTCAGTTGCCCTGTCAAAGCCATACATGACAGTTAGC
AGCGAGTTCCCCGCGGCCAGGCTCTCCAATGGCAAGTATCCCAAGGCTCCGGAAGGGGGC
GAAGGTGCCCAGCCAGTGCCCGGGCATGCCCGGAAGACAGCGGTTCAAGACAGAAAAGAT
GGCAGCTCACCTCCTCTGTTGGAGAAGCAGACCGTTACCAAAGACGTCACAGATAAGCCA
CTAGACTTGTCTTCTAAAGTGGTGGATGTAGATGCTTCCAAAGCTGACCACATGAAAAAG
ATGGCTCCCACGGTCCTGGTTCACAGCAGGGCTGGAAGTGGCTTAGTGCTCTCCGGAAGT
GAGATTCCGAAAGAAACACTATCTCCTCCAGGAAATGGTTGTGCTATCTATAGATCTGAA
ATCATCAGCACTGCTCCCTCATCCTGGGTGGTGCCCGGGCCAAGTCCTAACGAAGAGAAC
AATGGCAAAAGCATGTCGCTGAAAAACAAGGCATTGGACTGGGCGATACCACAGCAGCGG
AGTTCATCATGCCCGCGCATGGGCGGCACCGATGCTGTCATCACTAACGTTTCAGGGTCA
GTGTCGAGTGCAGGCCGCCCAGCCTCCGCATCACCCGCCCCCAATGCCAATGCAGATGGC
ACCAAAACCAGCAGGAGCTCTGTAGAAACCACACCATCCGTTATTCAGCACGTGGGCCAG
CCCCCGGCCACTCCTGCCAAGCACAGTAGCAGCACCAGCAGCAAGGGCGCCAAAGCCAGC
AACCCAGAACCGAGTTTCAAAGCAAACGAGAACGGCCTTCCACCAAGCTCTATATTTCTG
TCTCCAAATGAGGCATTCAGGTCCCCACCAATTCCCTACCCCAGGAGTTACCTCCCTTAC
CCAGCCCCTGAGGGCATTGCTGTAAGTCCCCTCTCCTTACATGGCAAAGGACCTGTCTAC
CCTCACCCAGTTTTGTTACCCAATGGCAGTCTGTTTCCTGGGCACCTTGCCCCAAAGCCT
GGGCTGCCCTATGGGCTTCCCACCGGCCGTCCAGAGTTTGTGACCTACCAAGATGCCCTG
GGGTTGGGCATGGTGCATCCCATGTTGATACCACACACGCCCATAGAGATTACTAAAGAG
GAGAAACCAGAGAGGAGATCCCGGTCCCATGAGAGAGCCCGTTACGAGGACCCAACCCTC
CGGAATCGGTTTTCCGAGATTTTGGAAACTAGCAGCACCAAGTTACATCCAGATGTCCCC
ACCGACAAGAACCTAAAGCCGAACCCCAACTGGAATCAAGGGAAGACTGTTGTCAAAAGC
GACAAGCTTGTCTACGTAGACCTTCTCCGAGAAGAACCAGATGCTAAAACTGACACAAAC
GTGTCCAAACCCAGCTTTGCAGCAGAGAGTGTTGGCCAGAGCGCTGAGCCCCCCAAGCCC
TCAGTTGAGCCGGCCCTGCAGCAGCACCGTGATTTCATCGCCCTGAGAGAGGAGTTGGGG
CGCATCAGTGACTTCCACGAAACTTATACTTTCAAACAGCCAGTCTTCACCGTAAGCAAG
GACAGTGTTCTGGCAGGTACCAACAAAGAGAACCTAGGGTTGCCAGTCTCGACTCCATTC
CTGGAGCCACCTCTGGGGAGCGATGGCCCTGCTGTAACTTTTGGTAAAACCCAAGAGGAT
CCCAAACCATTTTGTGTGGGCAGTGCCCCACCAAGTGTGGATGTGACCCCCACCTATACC
AAAGATGGAGCTGATGAGGCTGAATCAAATGATGGCAAAGTTCTGAAACCGAAGCCATCT
AAGCTGGCAAAGAGAATCGCCAACTCAGCGGGTTACGTGGGTGACCGATTCAAATGTGTC
ACTACCGAACTGTATGCAGATTCCAGTCAGCTCAGCCGGGAGCAACGGGCATTGCAGATG
GAAGGATTACAAGAGGACAGTATTTTATGTCTACCCGCTGCTTACTGTGAGCGTGCAATG
ATGCGCTTCTCAGAGTTGGAGATGAAAGAAAGAGAAGGTGGCCACCCAGCAACCAAAGAC
TCCGAGATGTGCAAATTCAGCCCAGCCGACTGGGAAAGGTTGAAAGGAAATCAGGACAAA
AAGCCAAAGTCGGTCACCCTGGAGGAGGCCATTGCAGAACAGAACGAAAGTGAGAGATGC
GAGTATAGTGTTGGAAACAAGCACCGTGATCCCTTTGAAGCCCCAGAGGACAAAGATCTT
CCTGTGGAGAAGTACTTTGTGGAGAGGCAGCCTGTGAGCGAGCCTCCCGCAGACCAGGTG
GCCTCGGACATGCCTCACAGCCCCACCCTCCGGGTGGACAGGAAACGCAAAGTCTCAGGT
GACAGCAGCCACACTGAGACCACTGCGGAGGAGGTGCCAGAGGACCCTCTGCTGAAAGCC
AAACGCCGACGAGTCTCTAAAGATGACTGGCCTGAGAGGGAAATGACAAACAGTTCCTCT
AACCACTTAGAAGACCCACATTATAGTGAGCTGACCAACCTGAAGGTGTGCATTGAATTA
ACAGGGCTCCATCCTAAAAAACAACGCCACTTGCTGCACCTTAGAGAACGATGGGAGCAG
CAGGTGTCGGCAGCAGATGGCAAACCTGGCCGGCAAAGCAGGAAGGAAGTGACCCAGGCC
ACTCAGCCTGAGGCCATTCCTCAGGGGACTAACATCACTGAAGAGAAACCTGGCAGGAAA
AGGGCAGAGGCCAAAGGCAACAGAAGCTGGTCGGAAGAGTCTCTTAAACCCAGTGACAAT
GAACAAGGCTTGCCTGTGTTCTCCGGCTCTCCGCCCATGAAGAGTCTTTCATCCACCAGT
GCAGGCGGCAAAAAGCAGGCTCAGCCAAGCTGCGCACCAGCCTCCAGGCCGCCTGCCAAA
CAGCAGAAAATTAAAGAAAACCAGAAGACAGATGTGCTGTGTGCAGACGAAGAAGAGGAT
TGCCAGGCTGCCTCCCTGCTGCAGAAATACACCGACAACAGCGAGAAGCCATCCGGGAAG
AGACTGTGCAAAACCAAACACTTGATCCCTCAGGAGTCCAGGCGGGGATTGCCACTGACA
GGGGAATACTACGTGGAGAATGCCGATGGCAAGGTGACTGTCCGGAGATTCAGAAAGCGG
CCGGAGCCCAGTTCGGACTATGATCTGTCACCAGCCAAGCAGGAGCCAAAGCCCTTCGAC
CGCTTGCAGCAACTGCTACCAGCCTCCCAGTCCACACAGCTGCCATGCTCAAGTTCCCCT
CAGGAGACCACCCAGTCTCGCCCTATGCCGCCGGAAGCACGGAGACTTATTGTCAATAAG
AACGCTGGCGAGACCCTTCTGCAGCGGGCAGCCAGGCTTGGCTATGAGGAAGTGGTCCTG
TACTGCTTAGAGAACAAGATTTGTGATGTAAATCATCGGGACAACGCAGGTTACTGCGCC
CTGCATGAAGCTTGTGCTAGGGGCTGGCTCAACATTGTGCGACACCTCCTTGAATATGGC
GCTGATGTCAACTGTAGTGCCCAGGATGGAACCAGGCCTCTGCACGATGCTGTTGAGAAC
GATCACTTGGAAATTGTCCGACTACTTCTCTCTTATGGTGCTGACCCCACCTTGGCTACG
TACTCAGGTAGAACCATCATGAAAATGACCCACAGTGAACTTATGGAAAAGTTCTTAACA
GATTATTTAAATGACCTCCAGGGTCGCAATGATGATGACGCCAGTGGCACTTGGGACTTC
TATGGCAGCTCTGTTTGTGAACCAGATGATGAAAGTGGCTATGATGTTTTAGCCAACCCC
CCAGGACCAGAAGACCAGGATGATGATGACGATGCCTATAGCGATGTGTTTGAATTTGAA
TTTTCAGAGACCCCCCTCTTACCGTGTTATAACATCCAAGTATCTGTGGCTCAGGGGCCA
CGAAACTGGCTACTGCTTTCGGATGTCCTTAAGAAATTGAAAATGTCCTCCCGCATATTT
CGCTGCAATTTTCCAAACGTGGAAATTGTCACCATTGCAGAGGCAGAATTTTATCGGCAG
GTTTCTGCAAGTCTCTTGTTCTCTTGCTCCAAAGACCTGGAAGCCTTCAACCCTGAAAGT
AAGGAGCTGTTAGATCTGGTGGAATTCACGAACGAAATTCAGACTCTGCTGGGCTCCTCT
GTAGAGTGGCTCCACCCCAGTGATCTGGCCTCAGACAACTACTGGTTG
Protein sequence predicted based on nucleotide sequence
MLSATPLYGNVHSWMNSERVRMCGASEDRKILVNDGDASKARLELREENPLNHNVVDAST AHRIDGLAALSMDRTGLIREGLRVPGNIVYSSLCGLGSEKGREAATSTLGGLGFSSERNP EMQFKPNTPETVEASAVSGKPPNGFSAIYKTPPGIQKSAVATAEALGLDRPASDKQSPLN INGASYLRLPWVNPYMEGATPAIYPFLDSPNKYSLNMYKALLPQQSYSLAQPLYSPVCTN GERFLYLPPPHYVGPHIPSSLASPMRLSTPSASPAIPPLVHCADKSLPWKMGVSPGNPVD SHAYPHIQNSKQPRVPSAKAVTSGLPGDTALLLPPSPRPSPRVHLPTQPAADTYSEFHKH YARISTSPSVALSKPYMTVSSEFPAARLSNGKYPKAPEGGEGAQPVPGHARKTAVQDRKD GSSPPLLEKQTVTKDVTDKPLDLSSKVVDVDASKADHMKKMAPTVLVHSRAGSGLVLSGS EIPKETLSPPGNGCAIYRSEIISTAPSSWVVPGPSPNEENNGKSMSLKNKALDWAIPQQR SSSCPRMGGTDAVITNVSGSVSSAGRPASASPAPNANADGTKTSRSSVETTPSVIQHVGQ PPATPAKHSSSTSSKGAKASNPEPSFKANENGLPPSSIFLSPNEAFRSPPIPYPRSYLPY PAPEGIAVSPLSLHGKGPVYPHPVLLPNGSLFPGHLAPKPGLPYGLPTGRPEFVTYQDAL GLGMVHPMLIPHTPIEITKEEKPERRSRSHERARYEDPTLRNRFSEILETSSTKLHPDVP TDKNLKPNPNWNQGKTVVKSDKLVYVDLLREEPDAKTDTNVSKPSFAAESVGQSAEPPKP SVEPALQQHRDFIALREELGRISDFHETYTFKQPVFTVSKDSVLAGTNKENLGLPVSTPF LEPPLGSDGPAVTFGKTQEDPKPFCVGSAPPSVDVTPTYTKDGADEAESNDGKVLKPKPS KLAKRIANSAGYVGDRFKCVTTELYADSSQLSREQRALQMEGLQEDSILCLPAAYCERAM MRFSELEMKEREGGHPATKDSEMCKFSPADWERLKGNQDKKPKSVTLEEAIAEQNESERC EYSVGNKHRDPFEAPEDKDLPVEKYFVERQPVSEPPADQVASDMPHSPTLRVDRKRKVSG DSSHTETTAEEVPEDPLLKAKRRRVSKDDWPEREMTNSSSNHLEDPHYSELTNLKVCIEL TGLHPKKQRHLLHLRERWEQQVSAADGKPGRQSRKEVTQATQPEAIPQGTNITEEKPGRK RAEAKGNRSWSEESLKPSDNEQGLPVFSGSPPMKSLSSTSAGGKKQAQPSCAPASRPPAK QQKIKENQKTDVLCADEEEDCQAASLLQKYTDNSEKPSGKRLCKTKHLIPQESRRGLPLT GEYYVENADGKVTVRRFRKRPEPSSDYDLSPAKQEPKPFDRLQQLLPASQSTQLPCSSSP QETTQSRPMPPEARRLIVNKNAGETLLQRAARLGYEEVVLYCLENKICDVNHRDNAGYCA LHEACARGWLNIVRHLLEYGADVNCSAQDGTRPLHDAVENDHLEIVRLLLSYGADPTLAT YSGRTIMKMTHSELMEKFLTDYLNDLQGRNDDDASGTWDFYGSSVCEPDDESGYDVLANP PGPEDQDDDDDAYSDVFEFEFSETPLLPCYNIQVSVAQGPRNWLLLSDVLKKLKMSSRIF RCNFPNVEIVTIAEAEFYRQVSASLLFSCSKDLEAFNPESKELLDLVEFTNEIQTLLGSS VEWLHPSDLASDNYW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
