Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
antibiotic resistance: Kan
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00832974
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5490nts
Open reading frame: 1 to 5490
ATGGCCCCCTGGCGCAAAGCTGACAAGGAGCGGCACGGCGTGGCCATATACAACTTCCAA
GGCAGCGGAGCCCCCCAGCTCTCCCTGCAGATCGGCGATGTGGTGCGAATACAGGAGACG
TGTGGAGACTGGTATAGGGGATACCTCATAAAGCACAAAATGTTACAGGGCATTTTTCCT
AAGTCATTTATCCACATCAAGGAAGTGACAGTTGAGAAAAGAAGAAATACTGAGAACATC
ATTCCTGCAGAAATTCCTCTGGCACAAGAAGTGACAACGACACTTTGGGAATGGGGAAGC
ATCTGGAAACAACTCTATGTGGCCAGCAAAAAGGAGCGTTTTCTCCAGGTGCAGTCCATG
ATGTACGATCTGATGGAGTGGAGGTCCCAGCTTCTCTCAGGAACCTTACCCAAGGATGAG
CTGAAGGAACTGAAGCAGAAAGTCACGTCCAAAATTGACTATGGCAACAAAATCCTTGAG
CTTGATTTGATTGTCAGAGATGAAGACGGAAATATCTTGGACCCTGATAATACCAGTGTC
ATCAGCTTGTTCCATGCACATGAGGAAGCAACTGATAAAATCACAGAGCGTATCAAAGAA
GAAATGTCAAAAGACCAGCCAGATTATGCAATGTATTCCCGGATCTCCTCATCCCCCACC
CATAGCCTCTATGTGTTTGTGAGAAACTTTGTGTGCAGAATTGGGGAAGATGCTGAGCTC
TTCATGTCTCTCTACGACCCCAACAAGCAAACGGTCATAAGTGAGAACTACCTAGTGCGA
TGGGGCAGCCGGGGCTTCCCTAAGGAGATTGAGATGCTCAACAATCTGAAGGTGGTCTTC
ACGGATCTTGGAAACAAAGACCTCAACAGGGATAAAATTTACTTGATTTGTCAAATAGTC
CGGGTCGGCAAGATGGATCTTAAGGATACTGGTGCAAAGAAGTGCACGCAGGGACTGAGG
AGGCCCTTTGGGGTGGCAGTTATGGATATAACAGACATCATCAAGGGGAAAGCAGAGAGT
GATGAAGAAAAGCAGCACTTCATTCCTTTTCACCCGGTTACAGCTGAGAATGACTTCCTA
CACAGCCTGCTGGGCAAAGTCATAGCCTCCAAGGGGGACAGTGGAGGGCAAGGCCTCTGG
GTGACCATGAAGATGCTGGTGGGTGACATCATTCAGATTCGCAAGGACTATCCACACCTG
GTGGACAGGACCACCGTGGTGGCCAGGAAGCTGGGATTCCCAGAGATCATCATGCCAGGG
GATGTCAGGAACGACATCTACATTACTCTCTTACAAGGTGACTTTGACAAGTACAACAAG
ACCACACAGAGGAATGTGGAAGTCATCATGTGTGTGTGCGCGGAGGATGGCAAAACGCTG
CCTAATGCAATTTGCGTGGGAGCAGGGGACAAGCCCATGAATGAGTATCGCTCCGTTGTG
TACTATCAAGTCAAACAGCCACGCTGGATGGAAACAGTCAAGGTGGCTGTCCCTATTGAA
GACATGCAGAGGATCCATCTGCGATTCATGTTTCGACATCGGTCATCTCTGGAATCTAAA
GATAAAGGAGAAAAGAACTTTGCCATGTCCTATGTGAAGCTGATGAAAGAAGATGGGACT
ACTCTACACGATGGATTCCATGACTTAGTTGTCCTCAAGGGGGACAGCAAGAAGATGGAG
GATGCCAGCGCATACCTGACCCTTCCTTCTTATCGACACCATGTGGAAAACAAGGGGGCC
ACGCTGAGCAGGAGCTCCAGCAGTGTTGGGGGGCTTTCTGTCAGCTCCCGGGATGTGTTC
TCCATTTCCACCCTGGTGTGCTCCACAAAGCTCACTCAGAATGTGGGCTTGCTGGGTTTG
CTGAAGTGGCGTATGAAGCCTCAACTGCTACAGGAGAATTTAGAAAAGTTGAAGATTGTG
GATGGAGAGGAAGTGGTGAAGTTTCTCCAGGATACTCTGGATGCCCTCTTCAACATCATG
ATGGAGCATTCTCAAAGTGATGAATATGACATCCTCGTCTTTGATGCCTTGATTTACATA
ATAGGACTCATTGCAGACCGGAAATTTCAGCATTTCAACACCGTTCTGGAGGCTTACATC
CAACAGCATTTCAGTGCGACCTTGGCTTACAAGAAATTGATGACAGTGCTGAAGACTTAC
TTGGATACCTCCAGCAGAGGGGAGCAATGTGAGCCAATCCTAAGAACGCTGAAGGCTTTG
GAATATGTGTTCAAGTTCATTGTTCGGTCGAGGACATTATTTTCACAGCTTTATGAAGGC
AAAGAACAGATGGAGTTTGAAGAATCCATGAGACGGCTCTTTGAATCCATCAACAATCTG
ATGAAAAGTCAATACAAAACTACCATCCTTTTGCAGGTGGCGGCTTTGAAATACATCCCA
TCTGTCCTGCATGATGTAGAAATGGTCTTTGATGCGAAGTTACTCAGCCAACTCCTGTAT
GAGTTCTACACCTGCATCCCTCCTGTGAAACTCCAGAAGCAGAAAGTACAGTCTATGAAT
GAGATAGTCCAGAGCAACCTCTTTAAAAAGCAAGAATGCCGGGACATTCTGCTTCCTGTC
ATCACCAAAGAGCTGAAGGAGCTGCTGGAGCAGAAGGATGACATGCAACACCAGGTCCTG
GAGAGGAAGTACTGCGTTGAATTGCTCAACAGCATCTTGGAAGTCCTTAGCTACCAGGAT
GCGGCCTTCACCTACCACCATATCCAGGAGATCATGGTCCAGCTGCTGCGGACAGTGAAC
CGGACAGTCATCACCATGGGCCGGGATCACATTCTGATTAGTCACTTTGTGGCATGTATG
ACAGCCATCTTAAACCAGATGGGTGACCAGCACTACTCCTTCTACATTGAGACCTTCCAG
ACCAGCTCTGAACTTGTGGACTTCTTGATGGAGACCTTCATCATGTTCAAGGACCTCATT
GGAAAGAACGTGTACCCTGGAGACTGGATGGCCATGAGCATGGTTCAAAACAGGGTCTTC
CTGAGAGCTATCAACAAGTTTGCAGAAACCATGAACCAGAAGTTCCTAGAACACACGAAC
TTTGAGTTCCAGCTGTGGAACAACTATTTTCATCTGGCAGTGGCTTTTATCACCCAGGAT
TCTCTGCAGCTGGAGCAGTTCTCACACGCCAAATACAACAAAATCCTGAATAAGTATGGG
GACATGAGACGGCTAATTGGCTTCTCCATCCGTGATATGTGGTACAAGCTTGGTCAGAAC
AAAATCTGCTTCATCCCAGGCATGGTAGGACCTATATTAGAGATGACACTTATCCCTGAG
GCTGAGCTCCGGAAAGCCACCATACCAATCTTCTTCGACATGATGCTGTGTGAATATCAA
AGAAGTGGGGATTTCAAAAAGTTTGAAAACGAAATCATCCTGAAGCTGGACCACGAGGTA
GAAGGGGGCCGAGGCGACGAGCAGTACATGCAGCTCCTGGAGTCAATCCTGATGGAATGT
GCTGCAGAGCACCCAACCATTGCCAAGTCGGTGGAGAACTTCGTGAACCTGGTCAAAGGC
CTCCTGGAGAAGCTGCTGGATTACCGGGGTGTGATGACAGATGAGAGCAAAGACAACCGC
ATGAGCTGCACCGTGAACCTGCTGAATTTCTACAAAGATAACAACAGGGAGGAGATGTAC
ATAAGGTACCTGTACAAACTCCGCGATCTTCACCTGGACTGTGACAATTACACAGAGGCT
GCCTACACGCTCCTTCTCCACACCTGGCTTCTCAAGTGGTCGGATGAGCAGTGTGCATCA
CAGGTCATGCAGACAGGCCAGCAGCACCCCCAGACACACCGGCAGCTGAAGGAGACGCTC
TACGAGACCATCATAGGCTACTTTGACAAAGGAAAGATGTGGGAAGAGGCCATAAGTCTG
TGCAAGGAGCTGGCGGAACAGTACGAGATGGAGATCTTTGACTATGAGCTGCTCAGCCAG
AACCTGATCCAGCAGGCAAAATTCTATGAAAGCATCATGAAAATCCTCAGGCCCAAACCA
GACTACTTTGCTGTTGGATACTACGGCCAGGGATTCCCCTCCTTCCTGCGGAACAAAGTG
TTCATCTACCGCGGGAAGGAATATGAGCGAAGAGAAGATTTCCAGATGCAGCTGATGACC
CAGTTCCCCAATGCAGAGAAGATGAACACCACCTCTGCCCCGGGAGATGATGTGAAGAAT
GCCCCAGGCCAGTATATCCAGTGCTTCACTGTCCAGCCTGTCTTGGATGAACATCCCAGG
TTCAAGAATAAGCCAGTGCCTGACCAGATTATAAACTTCTACAAATCCAACTACGTGCAA
AGGTTCCACTACTCCCGGCCCGTGCGCAGGGGGACCGTAGACCCAGAGAATGAGTTTGCT
TCCATGTGGATTGAGAGAACCTCCTTCGTGACTGCATACAAGCTGCCGGGGATCCTGCGC
TGGTTTGAGGTGGTGCACATGTCGCAGACCACAATTAGTCCTCTGGAGAATGCCATAGAA
ACCATGTCCACGGCCAATGAGAAGATCCTGATGATGATAAACCAGTACCAGAGTGATGAG
ACCCTCCCCATCAACCCACTCTCCATGCTCCTGAACGGGATTGTGGACCCTGCTGTCATG
GGAGGCTTCGCCAAGTATGAGAAGGCCTTCTTCACTGAAGAGTATGTCAGGGACCACCCT
GAGGACCAGGACAAGCTGACCCACCTCAAGGACCTGATTGCATGGCAGATCCCCTTCTTG
GGAGCTGGGATTAAGATCCATGAGAAAAGGGTGTCAGATAACTTGCGACCCTTCCATGAC
CGGATGGAGGAATGTTTCAAGAACCTGAAAATGAAGGTGGAGAAGGAGTACGGTGTCCGA
GAGATGCCTGACTTTGACGACAGGAGAGTGGGCCGTCCCAGGTCTATGCTGCGCTCATAC
AGACAGATGTCCATCATCTCTCTGGCTTCCATGAATTCTGACTGCAGCACCCCCAGCAAG
CCTACCTCAGAGAGCTTTGACCTGGAATTAGCATCACCCAAGACGCCGAGAGTGGAGCAG
GAGGAACCGATCTCCCCGGGGAGCACCCTGCCTGAGGTCAAGCTGCGGAGGTCCAAGAAG
AGGACAAAGAGAAGCAGCGTAGTTTTTGCGGATGAGAAAGCAGCTGCAGAGTCGGACCTG
AAGCGGCTTTCCAGGAAGCATGAGTTCATGAGTGACACCAACCTCTCGGAGCATGCGGCC
ATCCCCCTCAAGGCGTCTGTCCTCTCTCAAATGAGCTTTGCCAGCCAGTCCATGCCTACC
ATCCCAGCCCTGGCGCTCTCAGTGGCAGGCATCCCTGGGTTGGATGAGGCCAACACATCT
CCCCGCCTCAGCCAGACCTTCCTCCAACTCTCAGATGGTGACAAGAAGACACTCACACGG
AAGAAGGTCAATCAGTTCTTCAAGACAATGCTGGCCAGCAAATCGGCTGAAGAAGGCAAA
CAGATCCCAGACTCGCTGTCCACGGACCTGTTG
Protein sequence predicted based on nucleotide sequence
MAPWRKADKERHGVAIYNFQGSGAPQLSLQIGDVVRIQETCGDWYRGYLIKHKMLQGIFP KSFIHIKEVTVEKRRNTENIIPAEIPLAQEVTTTLWEWGSIWKQLYVASKKERFLQVQSM MYDLMEWRSQLLSGTLPKDELKELKQKVTSKIDYGNKILELDLIVRDEDGNILDPDNTSV ISLFHAHEEATDKITERIKEEMSKDQPDYAMYSRISSSPTHSLYVFVRNFVCRIGEDAEL FMSLYDPNKQTVISENYLVRWGSRGFPKEIEMLNNLKVVFTDLGNKDLNRDKIYLICQIV RVGKMDLKDTGAKKCTQGLRRPFGVAVMDITDIIKGKAESDEEKQHFIPFHPVTAENDFL HSLLGKVIASKGDSGGQGLWVTMKMLVGDIIQIRKDYPHLVDRTTVVARKLGFPEIIMPG DVRNDIYITLLQGDFDKYNKTTQRNVEVIMCVCAEDGKTLPNAICVGAGDKPMNEYRSVV YYQVKQPRWMETVKVAVPIEDMQRIHLRFMFRHRSSLESKDKGEKNFAMSYVKLMKEDGT TLHDGFHDLVVLKGDSKKMEDASAYLTLPSYRHHVENKGATLSRSSSSVGGLSVSSRDVF SISTLVCSTKLTQNVGLLGLLKWRMKPQLLQENLEKLKIVDGEEVVKFLQDTLDALFNIM MEHSQSDEYDILVFDALIYIIGLIADRKFQHFNTVLEAYIQQHFSATLAYKKLMTVLKTY LDTSSRGEQCEPILRTLKALEYVFKFIVRSRTLFSQLYEGKEQMEFEESMRRLFESINNL MKSQYKTTILLQVAALKYIPSVLHDVEMVFDAKLLSQLLYEFYTCIPPVKLQKQKVQSMN EIVQSNLFKKQECRDILLPVITKELKELLEQKDDMQHQVLERKYCVELLNSILEVLSYQD AAFTYHHIQEIMVQLLRTVNRTVITMGRDHILISHFVACMTAILNQMGDQHYSFYIETFQ TSSELVDFLMETFIMFKDLIGKNVYPGDWMAMSMVQNRVFLRAINKFAETMNQKFLEHTN FEFQLWNNYFHLAVAFITQDSLQLEQFSHAKYNKILNKYGDMRRLIGFSIRDMWYKLGQN KICFIPGMVGPILEMTLIPEAELRKATIPIFFDMMLCEYQRSGDFKKFENEIILKLDHEV EGGRGDEQYMQLLESILMECAAEHPTIAKSVENFVNLVKGLLEKLLDYRGVMTDESKDNR MSCTVNLLNFYKDNNREEMYIRYLYKLRDLHLDCDNYTEAAYTLLLHTWLLKWSDEQCAS QVMQTGQQHPQTHRQLKETLYETIIGYFDKGKMWEEAISLCKELAEQYEMEIFDYELLSQ NLIQQAKFYESIMKILRPKPDYFAVGYYGQGFPSFLRNKVFIYRGKEYERREDFQMQLMT QFPNAEKMNTTSAPGDDVKNAPGQYIQCFTVQPVLDEHPRFKNKPVPDQIINFYKSNYVQ RFHYSRPVRRGTVDPENEFASMWIERTSFVTAYKLPGILRWFEVVHMSQTTISPLENAIE TMSTANEKILMMINQYQSDETLPINPLSMLLNGIVDPAVMGGFAKYEKAFFTEEYVRDHP EDQDKLTHLKDLIAWQIPFLGAGIKIHEKRVSDNLRPFHDRMEECFKNLKMKVEKEYGVR EMPDFDDRRVGRPRSMLRSYRQMSIISLASMNSDCSTPSKPTSESFDLELASPKTPRVEQ EEPISPGSTLPEVKLRRSKKRTKRSSVVFADEKAAAESDLKRLSRKHEFMSDTNLSEHAA IPLKASVLSQMSFASQSMPTIPALALSVAGIPGLDEANTSPRLSQTFLQLSDGDKKTLTR KKVNQFFKTMLASKSAEEGKQIPDSLSTDL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
